FastQCFastQC Report
Fri 10 Feb 2017
SRR2031723_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031723_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110203
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT22372.029890293367694No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12121.0997885719989473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11521.0453435931871182No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT10850.9845467001805759RNA PCR Primer, Index 39 (95% over 23bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG8710.7903596090850522RNA PCR Primer, Index 39 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT7700.6987105614184732RNA PCR Primer, Index 39 (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5790.5253940455341506No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT3900.353892362276889RNA PCR Primer, Index 39 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3700.33574403600627933No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.31215121185448674No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.2994473834650599No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA3020.27403972668620635RNA PCR Primer, Index 39 (96% over 30bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2430.22050216418790777No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG2280.20689091948495048Illumina PCR Primer Index 11 (96% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.17150168325726162No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1260.11433445550484106No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.10435287605600574No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTAT554.9376464E-543.1764561
CGTCAGA459.5930416E-442.216982
GCCTGCG250.001613967537.9952888-89
GGTATCA8300.037.1941761
ACTATAC600.003937997431.6627353
TATACAC7050.030.9890583
TTATACA7350.030.3703772
GTATCAA12200.030.3650841
CTTATAC7250.030.1341881
CATGGGG2700.028.1446534
TTCCGAG350.00834514427.16413770-71
GAGTAGT350.00838219727.1394866
GACTTGT350.00838219727.13948646-47
GTACATG7350.027.1394841
TACATGG7600.026.2467422
ACATGGG7550.025.7914983
TATCAAC14600.025.3735622
TACACAT8800.025.3661695
ATACACA9000.025.3301874
ATCAACG14650.025.2869633
Produced by FastQC (version 0.11.5)