##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031723_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 110203 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.38311116757257 33.0 31.0 34.0 31.0 34.0 2 32.5801021750769 34.0 31.0 34.0 31.0 34.0 3 32.58442147672931 34.0 31.0 34.0 31.0 34.0 4 36.11214758218923 37.0 35.0 37.0 35.0 37.0 5 36.05811094071849 37.0 35.0 37.0 35.0 37.0 6 36.11335444588623 37.0 36.0 37.0 35.0 37.0 7 36.05649573968041 37.0 35.0 37.0 35.0 37.0 8 36.049889748917906 37.0 35.0 37.0 35.0 37.0 9 37.82727330471947 39.0 38.0 39.0 35.0 39.0 10-11 37.796089943104995 39.0 38.0 39.0 35.0 39.0 12-13 37.72809723873216 39.0 38.0 39.0 35.0 39.0 14-15 38.976393564603505 40.0 38.0 41.0 35.5 41.0 16-17 38.917779007831 40.0 38.0 41.0 35.5 41.0 18-19 39.01762656189033 40.0 38.5 41.0 35.0 41.0 20-21 39.07679464261409 40.0 39.0 41.0 35.0 41.0 22-23 38.96080415233705 40.0 38.5 41.0 35.0 41.0 24-25 38.83529486493108 40.0 38.0 41.0 35.0 41.0 26-27 38.60819124706224 40.0 38.0 41.0 34.5 41.0 28-29 38.456879576781034 40.0 38.0 41.0 34.0 41.0 30-31 38.174251154687255 40.0 37.5 41.0 34.0 41.0 32-33 37.76656261626272 40.0 36.5 41.0 33.0 41.0 34-35 37.50254530275946 40.0 36.0 41.0 33.0 41.0 36-37 37.35748573087847 40.0 36.0 41.0 33.0 41.0 38-39 37.294874005244864 40.0 36.0 41.0 33.0 41.0 40-41 37.16539023438564 40.0 36.0 41.0 32.5 41.0 42-43 36.94326379499651 40.0 36.0 41.0 31.5 41.0 44-45 36.559521973086035 40.0 35.5 41.0 30.5 41.0 46-47 36.04530275945301 40.0 35.0 41.0 29.0 41.0 48-49 35.52816166529042 40.0 35.0 41.0 26.5 41.0 50-51 35.09639483498634 39.5 35.0 41.0 23.0 41.0 52-53 35.163117156520244 40.0 35.0 41.0 23.0 41.0 54-55 35.04241717557598 39.5 35.0 41.0 22.5 41.0 56-57 34.62182517717304 39.0 35.0 41.0 18.5 41.0 58-59 34.25373174958939 39.0 35.0 41.0 10.5 41.0 60-61 33.73974846419789 39.0 34.5 41.0 2.0 41.0 62-63 33.23115069462719 38.0 34.0 40.5 2.0 41.0 64-65 32.64774098708746 38.0 33.5 40.0 2.0 41.0 66-67 31.981996860339557 37.0 33.0 40.0 2.0 41.0 68-69 31.3266381133 37.0 32.0 39.5 2.0 41.0 70-71 30.908958921263483 36.0 31.5 39.0 2.0 41.0 72-73 30.52275346406178 36.0 31.0 39.0 2.0 40.5 74-75 30.179641207589633 35.0 31.0 38.5 2.0 40.0 76-77 29.325698937415495 35.0 29.5 37.0 2.0 39.0 78-79 29.50873388201773 35.0 31.0 37.0 2.0 39.0 80-81 29.287097447437908 35.0 31.0 36.5 2.0 39.0 82-83 29.00610237470849 35.0 30.5 36.0 2.0 37.5 84-85 28.693946625772437 35.0 30.0 36.0 2.0 37.0 86-87 28.42195312287324 35.0 30.0 35.0 2.0 37.0 88-89 28.166306724862302 35.0 29.5 35.0 2.0 36.0 90-91 27.933078046877128 35.0 29.0 35.0 2.0 36.0 92-93 27.727811402593396 35.0 29.0 35.0 2.0 36.0 94-95 27.512431603495365 34.0 29.0 35.0 2.0 35.5 96-97 27.297655236245838 34.0 29.0 35.0 2.0 35.0 98-99 27.064748691051967 34.0 27.5 35.0 2.0 35.0 100-101 26.340657695344046 33.5 25.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29617716707575426 1101 2 0.24906035843965668 1101 3 0.48457174626047106 1101 4 0.04337231736337799 1101 5 0.05718223577622439 1101 6 0.2411198483514596 1101 7 0.2701491856900162 1101 8 0.12273602791290017 1101 9 0.1847082510785114 1101 10-11 0.11078689904962147 1101 12-13 0.20740629068039595 1101 14-15 0.6192721132778942 1101 16-17 0.12017631257589301 1101 18-19 0.23995275057092158 1101 20-21 0.4980991563008388 1101 22-23 0.3225231221240463 1101 24-25 -0.13602612582035079 1101 26-27 -0.4373552194249726 1101 28-29 -0.05526097056333157 1101 30-31 0.21815220536024071 1101 32-33 0.12654438098708454 1101 34-35 -0.07986461652419763 1101 36-37 0.6419122305649765 1101 38-39 0.420481349157086 1101 40-41 0.4076025636119951 1101 42-43 0.732933683891325 1101 44-45 0.6905858003787273 1101 46-47 0.8281578855727361 1101 48-49 -0.09169619245258076 1101 50-51 0.2474069269946071 1101 52-53 1.3008121263547494 1101 54-55 0.6025604097691186 1101 56-57 0.8749669433612368 1101 58-59 1.0980987283619186 1101 60-61 0.6290928608472512 1101 62-63 1.0560307642261932 1101 64-65 1.455927042126092 1101 66-67 0.7716976604755992 1101 68-69 0.3564508464439271 1101 70-71 0.6457767716916152 1101 72-73 -0.09380202055191589 1101 74-75 0.3431383503698804 1101 76-77 0.5611698341810794 1101 78-79 -0.20717196989653175 1101 80-81 -0.2506765486825202 1101 82-83 -0.2562222054641943 1101 84-85 0.29833169770600065 1101 86-87 0.058432705249085615 1101 88-89 0.32349508719482145 1101 90-91 0.06905096722985604 1101 92-93 0.5184886230653518 1101 94-95 0.5301986701993684 1101 96-97 0.43835318470711826 1101 98-99 0.1312101748263892 1101 100-101 0.5795313792115238 1102 1 0.13377707440807995 1102 2 0.16925032716431332 1102 3 -0.30542014531835804 1102 4 -0.12609368518497632 1102 5 0.12760966540671603 1102 6 0.240540675410422 1102 7 0.36420687131494134 1102 8 0.31224141422124774 1102 9 0.02914239911511629 1102 10-11 0.21474844196632858 1102 12-13 0.33204430759224834 1102 14-15 0.25578317548106355 1102 16-17 0.0743637329396094 1102 18-19 0.43050064817315103 1102 20-21 0.06991660098982067 1102 22-23 -0.05486596625774354 1102 24-25 -0.3175968428364513 1102 26-27 0.15328534584781295 1102 28-29 0.22778084572301083 1102 30-31 0.14175929443703694 1102 32-33 0.11108046346130607 1102 34-35 -0.15295624168346933 1102 36-37 0.06986311669957956 1102 38-39 0.18649548097685198 1102 40-41 0.556334174871111 1102 42-43 1.0288910567628662 1102 44-45 0.6623221608559646 1102 46-47 0.9883571210644533 1102 48-49 0.8895386042577158 1102 50-51 -0.539978686349535 1102 52-53 -0.5051649383967813 1102 54-55 -0.23139070803465245 1102 56-57 -0.5635828075943934 1102 58-59 -0.4249523546914844 1102 60-61 -0.11844565415332653 1102 62-63 0.3810626187379569 1102 64-65 0.3027357992209687 1102 66-67 -0.022406358984614627 1102 68-69 0.10022473732774273 1102 70-71 0.5492286424202142 1102 72-73 0.4332453557946607 1102 74-75 0.5478759850275914 1102 76-77 0.3243460185897433 1102 78-79 0.24713128505539927 1102 80-81 -0.17804826187607858 1102 82-83 0.44544581260600324 1102 84-85 0.9177571581484933 1102 86-87 0.8235780776572881 1102 88-89 0.5792578579581757 1102 90-91 0.25739800765612486 1102 92-93 0.7553124386566843 1102 94-95 1.0657598887792368 1102 96-97 1.0640337129128383 1102 98-99 1.1139508211833906 1102 100-101 1.5170386188732863 1103 1 0.28359778261068413 1103 2 0.26505938140008567 1103 3 0.2537461478237333 1103 4 0.059249210377551265 1103 5 -0.09975474517150218 1103 6 0.3156191155717849 1103 7 0.3453812325879113 1103 8 0.23570622820601272 1103 9 0.1408636003701602 1103 10-11 0.2544727808278324 1103 12-13 -0.08570558035037834 1103 14-15 0.15218613379571622 1103 16-17 0.17611182796426306 1103 18-19 -0.17248007795863174 1103 20-21 0.2547552383721623 1103 22-23 0.3499412950600558 1103 24-25 -0.10115802616230951 1103 26-27 0.34372685189890717 1103 28-29 0.5097732258216254 1103 30-31 0.14243163867728725 1103 32-33 -0.036499097273782866 1103 34-35 -0.07479622375429074 1103 36-37 -0.13385718809647784 1103 38-39 -0.43833643296308367 1103 40-41 0.009886393607111188 1103 42-43 0.669242916915266 1103 44-45 0.7283239537739377 1103 46-47 0.2977475289532947 1103 48-49 0.7622974567902148 1103 50-51 -0.0632305913248814 1103 52-53 -0.2379641315836949 1103 54-55 -0.2618142849060092 1103 56-57 0.3836381695459039 1103 58-59 0.6881695637307459 1103 60-61 -0.1549203291869432 1103 62-63 -0.8399145215578727 1103 64-65 -0.8661906911421013 1103 66-67 -0.9356179233055464 1103 68-69 -1.1524646396332585 1103 70-71 -0.7938910348545534 1103 72-73 -0.8609612780018416 1103 74-75 -0.9271352207097472 1103 76-77 -0.7967319733421228 1103 78-79 -1.0193971775174404 1103 80-81 -1.1472324841979074 1103 82-83 -0.7838235733195908 1103 84-85 -0.7916780726896988 1103 86-87 -0.9603753064753882 1103 88-89 -0.9014682738212976 1103 90-91 -0.7015889936885635 1103 92-93 -0.8736095694114461 1103 94-95 -0.8810128588675603 1103 96-97 -1.0486399760158918 1103 98-99 -0.5997986185297428 1103 100-101 -0.5885194268461227 1104 1 -0.05928759288338625 1104 2 0.09323241609485677 1104 3 -0.02842337347622248 1104 4 0.09592026878773652 1104 5 0.12323603216709955 1104 6 0.12172363906761063 1104 7 0.2035279168215567 1104 8 0.2639231803360076 1104 9 0.4057955283455641 1104 10-11 0.20907313216731183 1104 12-13 -0.2005496734553418 1104 14-15 -0.08853630801367984 1104 16-17 -0.12911434682713008 1104 18-19 -0.18576510211322272 1104 20-21 -0.08060927634017645 1104 22-23 -0.03362260480819401 1104 24-25 0.009019839450331801 1104 26-27 0.3491616345076096 1104 28-29 0.1613432741308003 1104 30-31 0.15363058465884194 1104 32-33 -0.014869760427060896 1104 34-35 0.272535792055983 1104 36-37 0.20518541181568395 1104 38-39 -0.03275891868992176 1104 40-41 0.15541779457328886 1104 42-43 0.46156834511025835 1104 44-45 0.23172755500362285 1104 46-47 0.42082614116233685 1104 48-49 -0.17528488840083156 1104 50-51 0.42320990055039687 1104 52-53 0.6874311253332976 1104 54-55 0.327393005388231 1104 56-57 0.014621920094867846 1104 58-59 0.1430992958343893 1104 60-61 0.808243922020786 1104 62-63 0.42886018199943976 1104 64-65 0.6938115047296591 1104 66-67 -0.42941458558047074 1104 68-69 -0.6442301205291763 1104 70-71 -0.38787434622916805 1104 72-73 -0.6048677338648183 1104 74-75 -0.3745440920316625 1104 76-77 -0.3185260005709374 1104 78-79 -0.21642177128116558 1104 80-81 -0.13394746004331992 1104 82-83 -0.10266415302973186 1104 84-85 0.13399166819743513 1104 86-87 0.4008455982237784 1104 88-89 0.17714393522569338 1104 90-91 0.20881943845899897 1104 92-93 0.15070971034262115 1104 94-95 -0.20150034239852133 1104 96-97 -0.2183259663540582 1104 98-99 -0.6146756497111241 1104 100-101 -0.7861478853441461 1105 1 0.2770760434802497 1105 2 0.23636372922617 1105 3 0.43592006086721113 1105 4 0.18359703646450498 1105 5 -0.02888517995410922 1105 6 0.18909737644134594 1105 7 0.24059862389226083 1105 8 0.08048883690165809 1105 9 0.10347229602233199 1105 10-11 0.2588206069147887 1105 12-13 0.35864224573657566 1105 14-15 0.7819687424913724 1105 16-17 0.7271987844859993 1105 18-19 -0.014653991002106181 1105 20-21 0.48888567315477616 1105 22-23 0.7566804254948352 1105 24-25 0.779928930359425 1105 26-27 0.6541616345076093 1105 28-29 0.7791210519085823 1105 30-31 0.9543881604164213 1105 32-33 0.996544380987082 1105 34-35 0.8738994284196266 1105 36-37 0.8883167249470034 1105 38-39 1.1934026974716971 1105 40-41 0.960973350128846 1105 42-43 0.7414168299587445 1105 44-45 0.8278891711652463 1105 46-47 1.7705736159098109 1105 48-49 2.225775717659779 1105 50-51 2.102856365196864 1105 52-53 2.0333402162423866 1105 54-55 2.16840310639833 1105 56-57 2.672551213024157 1105 58-59 2.258604346339439 1105 60-61 1.8626883664652283 1105 62-63 1.8220420001812556 1105 64-65 2.165983221901371 1105 66-67 2.015686424520542 1105 68-69 1.7897092734102173 1105 70-71 1.7826307042758884 1105 72-73 2.0366474176503253 1105 74-75 2.3030821705945996 1105 76-77 2.2071810701361336 1105 78-79 2.0562549963956016 1105 80-81 1.524941428845569 1105 82-83 1.4740025136369326 1105 84-85 1.726365405571169 1105 86-87 2.0433203456985254 1105 88-89 1.834618682700441 1105 90-91 2.077758832398395 1105 92-93 2.4174773871102957 1105 94-95 2.3263784454802696 1105 96-97 1.9422295892014994 1105 98-99 1.7119405119050377 1105 100-101 2.254306660110398 1106 1 0.4520760434802469 1106 2 0.0890560369184783 1106 3 0.3674585224056699 1106 4 0.2001354980029646 1106 5 0.4111148200458885 1106 6 0.26294353028750095 1106 7 0.006752470046109238 1106 8 -0.098741932329105 1106 9 0.10616460371463887 1106 10-11 0.21266676076093916 1106 12-13 0.1176807072750421 1106 14-15 0.4619687424913721 1106 16-17 0.47816032294754507 1106 18-19 0.23784600899789154 1106 20-21 0.2390779808470782 1106 22-23 0.30918042549483715 1106 24-25 0.08550585343634509 1106 26-27 -0.22295375010777718 1106 28-29 -0.40222510193757444 1106 30-31 -0.6071503011220472 1106 32-33 -0.6234556190129155 1106 34-35 -0.14879287927268336 1106 36-37 -0.08264481351454123 1106 38-39 -0.02717422560522209 1106 40-41 -0.2313343421788474 1106 42-43 -0.625698554656644 1106 44-45 -0.041533905757837886 1106 46-47 0.19845823129442408 1106 48-49 0.1711603330443907 1106 50-51 0.5705486728891671 1106 52-53 0.18218637008854444 1106 54-55 0.4284031063983349 1106 56-57 -0.0914872485142979 1106 58-59 -0.14774180750671206 1106 60-61 -0.19442701815015795 1106 62-63 -0.19526569212644063 1106 64-65 0.4782909142090688 1106 66-67 -0.22219819086407355 1106 68-69 -0.13971380351285845 1106 70-71 -0.3469846803395029 1106 72-73 0.008185879188790324 1106 74-75 0.23423601674844718 1106 76-77 0.20891183936690183 1106 78-79 0.3012549963956026 1106 80-81 0.41359527499941606 1106 82-83 0.12169482132923903 1106 84-85 -0.15748074827498115 1106 86-87 -0.01994888507070769 1106 88-89 0.19865714423890068 1106 90-91 0.5498742170137767 1106 92-93 0.4332466178795258 1106 94-95 0.24022459932642093 1106 96-97 0.7603065122784187 1106 98-99 0.5709789734434985 1106 100-101 -0.0628087245049862 1107 1 -0.13292395651975397 1107 2 -0.0936362707738283 1107 3 0.01592006086720943 1107 4 0.0335970364645064 1107 5 0.29111482004589107 1107 6 0.029097376441349354 1107 7 -0.14940137610773974 1107 8 0.02048883690166292 1107 9 -0.11652770397766687 1107 10-11 0.1438206069147796 1107 12-13 0.08864224573657964 1107 14-15 -0.7280312575086256 1107 16-17 -0.16280121551400129 1107 18-19 -0.13465399100211073 1107 20-21 -0.19611432684522612 1107 22-23 0.07168042549484 1107 24-25 0.3399289303594273 1107 26-27 -0.17583836549238896 1107 28-29 -0.2558789480914214 1107 30-31 0.20438816041642127 1107 32-33 -0.17345561901291262 1107 34-35 -0.5061005715803759 1107 36-37 -0.7066832750530025 1107 38-39 -1.126597302528296 1107 40-41 -0.8240266498711506 1107 42-43 -0.4785831700412544 1107 44-45 -0.7471108288347565 1107 46-47 -0.7644263840901857 1107 48-49 -0.7742242823402208 1107 50-51 -0.6871436348031423 1107 52-53 -1.106659783757614 1107 54-55 -1.321596893601665 1107 56-57 -1.5324487869758414 1107 58-59 -1.6313956536605616 1107 60-61 -1.8823116335347692 1107 62-63 -1.9129579998187474 1107 64-65 -1.9040167780986224 1107 66-67 -1.1143135754794606 1107 68-69 -0.6952907265897821 1107 70-71 -0.2323692957241157 1107 72-73 -0.3583525823496707 1107 74-75 -1.381917829405399 1107 76-77 -1.7778189298638658 1107 78-79 -1.3787450036043971 1107 80-81 -0.8400585711544295 1107 82-83 -0.6859974863630676 1107 84-85 -0.7136345944288287 1107 86-87 -0.6766796543014735 1107 88-89 -0.9003813172995621 1107 90-91 -0.4322411676016067 1107 92-93 -0.9275226128897067 1107 94-95 -0.7586215545197312 1107 96-97 -0.7427704107984994 1107 98-99 -0.1630594880949623 1107 100-101 -0.7556933398896 1108 1 0.13847955225217845 1108 2 0.05548653624371269 1108 3 0.1252183064812442 1108 4 0.08289528207853891 1108 5 0.05146569723886785 1108 6 -0.149674553383214 1108 7 -0.16834874452879234 1108 8 -5.637946772836244E-4 1108 9 -0.15986103731100343 1108 10-11 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>>Per sequence quality scores pass #Quality Count 5 3.0 6 9.0 7 25.0 8 31.0 9 80.0 10 151.0 11 275.0 12 373.0 13 509.0 14 706.0 15 945.0 16 1354.0 17 1985.0 18 2039.0 19 1304.0 20 1054.0 21 1129.0 22 1361.0 23 1356.0 24 1503.0 25 1625.0 26 1543.0 27 983.0 28 1049.0 29 1270.0 30 1568.0 31 1927.0 32 2261.0 33 3085.0 34 4238.0 35 6546.0 36 10077.0 37 19889.0 38 29765.0 39 8178.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.422963467211254 21.743589743589745 15.453596550941684 22.37985023825732 2 15.63296824950319 22.362367630645263 40.676751086631036 21.32791303322051 3 17.544146204243116 28.86517486071033 34.0347725086659 19.555906426380645 4 12.049341030388847 22.13084994372436 36.33409577751153 29.485713248375266 5 11.522372349210094 37.84742702104298 36.047113054998505 14.583087574748419 6 24.763391196246925 39.42451657395897 19.219984936889194 16.592107292904913 7 21.38235801203234 36.371968095242416 22.967614311770095 19.278059580955144 8 23.257080115786323 34.20142827327749 22.75164922914984 19.78984238178634 9 24.216219159188043 16.44238360117238 21.56656352367903 37.77483371596054 10-11 22.004845603114255 27.801874722103754 28.775532426521966 21.41774724826003 12-13 24.676279230147998 26.73792909448926 28.241971634166042 20.3438200411967 14-15 22.197217861582715 27.870838361932073 27.330471947224666 22.601471829260547 16-17 19.69873641705043 31.250198498219188 28.27385948594633 20.777205598784057 18-19 18.955559084412783 27.51208003448198 32.32367686758467 21.208684013520564 20-21 20.148725533787644 25.69666887471303 32.94874005244866 21.205865539050663 22-23 19.362903006270248 27.545983321688155 32.76181229186138 20.329301380180212 24-25 19.40282932406559 28.285073909058738 33.6492654464942 18.66283132038148 26-27 18.631694524600277 27.697319467886246 34.698099852997224 18.972886154516253 28-29 17.22280704938563 26.443817267727827 34.78410802766031 21.549267655226238 30-31 18.136076150377033 26.097293177136738 35.15648394326833 20.6101467292179 32-33 17.976370879195667 27.054163679754634 35.21410487917752 19.75536056187218 34-35 18.443046934552978 28.20519801081709 35.40600384768957 17.94575120694036 36-37 18.709788655069463 27.352335320647192 33.43814372181236 20.499732302470985 38-39 18.846129415714636 26.769234957306065 34.41058773354627 19.974047893433028 40-41 18.675535148770905 26.594557316951445 35.03625128172555 19.69365625255211 42-43 19.56298830340372 26.469787573841003 34.683266335762184 19.283957786993096 44-45 18.87335190512055 27.24835077085016 34.11250147455151 19.765795849477783 46-47 20.46496011905302 26.438481711024203 32.608458934874726 20.48809923504805 48-49 19.633313067702332 26.61043710243823 32.99093491102783 20.76531491883161 50-51 19.22134606135949 27.976552362458374 32.720071141438986 20.082030434743157 52-53 19.143761966552635 28.99739571518017 33.18920537553424 18.669636942732957 54-55 19.027612678420734 29.071803852889666 31.431539976225693 20.469043492463907 56-57 20.05027086376959 30.301352957723477 29.904358320553886 19.74401785795305 58-59 18.69050751794416 31.17247261871274 31.431086268068924 18.70593359527418 60-61 19.82141803877443 32.46779762525918 29.44560645726238 18.265177878704012 62-63 18.391821622063105 33.244398464512265 29.549971413792164 18.81380849963246 64-65 18.50119327410822 33.07048030417147 29.407174163573835 19.02115225814648 66-67 18.754594045209945 32.56077751662931 29.150521339056418 19.534107099104332 68-69 18.657156598517226 31.898656067659413 29.73348215501048 19.710705178812876 70-71 19.227244267397438 31.259584584811666 29.743291924902227 19.76987922288867 72-73 19.239442626967527 30.847448398528073 29.85375990852621 20.059349065978193 74-75 19.12527394742932 31.064163819428376 29.438855841262495 20.371706391879812 76-77 19.118701282202522 30.830482482010147 29.575502944619377 20.475313291167957 78-79 19.062548206491655 30.82583958694409 29.4329555456748 20.678656660889448 80-81 19.32149711656692 30.434171064034448 29.40012795092492 20.84420386847371 82-83 19.109759970960567 30.281319479105225 29.67330641136168 20.935614138572532 84-85 19.40944542497153 30.181077390053673 29.410670453668963 20.998806731305837 86-87 19.248743217001508 30.225857969909804 29.314803724070345 21.210595089018348 88-89 19.53131734761008 30.12862684603344 29.217123023524877 21.122932782831604 90-91 19.36154188179995 29.661624456684482 29.337223124597333 21.639610536918234 92-93 19.431412937941797 29.551827082747295 29.699282233695996 21.31747774561491 94-95 19.29030970118781 29.43567779461539 29.51280818126548 21.76120432293132 96-97 19.042131339437223 29.563623494823187 29.458363202453654 21.935881963285937 98-99 19.2186238124189 29.70381931526365 29.094942968884695 21.982613903432757 100-101 18.936503255370795 29.383180962319365 29.279281323018985 22.40103445929085 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 521.0 1 306.0 2 103.0 3 97.0 4 66.5 5 67.0 6 98.0 7 193.0 8 293.5 9 329.0 10 285.0 11 219.0 12 221.5 13 236.5 14 248.0 15 278.5 16 325.0 17 344.5 18 360.0 19 378.0 20 405.0 21 446.0 22 502.5 23 584.5 24 651.5 25 756.5 26 944.5 27 1184.0 28 1378.0 29 1594.0 30 1915.5 31 2209.5 32 2536.5 33 2988.0 34 3455.5 35 3826.5 36 4162.0 37 4474.5 38 4446.0 39 4354.0 40 4399.5 41 4429.5 42 4404.0 43 4357.5 44 4294.0 45 4131.5 46 3985.5 47 3992.5 48 4099.5 49 3847.0 50 3313.5 51 2844.5 52 2494.0 53 2203.5 54 1920.5 55 1716.0 56 1441.5 57 1125.5 58 904.0 59 737.0 60 568.5 61 418.0 62 314.0 63 218.5 64 155.0 65 110.5 66 74.0 67 45.5 68 37.5 69 30.0 70 17.0 71 12.0 72 10.5 73 9.0 74 6.0 75 4.0 76 2.0 77 2.5 78 2.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02540765677885357 2 0.0 3 9.074163135304848E-4 4 0.028129905719445025 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.537081567652424E-4 18-19 4.537081567652424E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.074163135304848E-4 28-29 0.008166746821774361 30-31 0.0 32-33 0.0 34-35 0.006351914194713392 36-37 0.003629665254121939 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0013611244702957269 62-63 0.009074163135304845 64-65 0.0018148326270609695 66-67 0.005444497881182907 68-69 0.003629665254121939 70-71 0.0 72-73 0.006805622351478635 74-75 0.007713038665009119 76-77 0.0018148326270609695 78-79 0.0 80-81 0.004083373410887181 82-83 0.007259330508243878 84-85 0.0013611244702957269 86-87 9.074163135304848E-4 88-89 4.537081567652424E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 4.537081567652424E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 110203.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.868524450332565 #Duplication Level Percentage of deduplicated Percentage of total 1 77.77809852344731 48.897942887217226 2 14.837694672574802 18.656479406186765 3 3.9288137061039508 7.409961616289938 4 1.411601691612661 3.549812618531256 5 0.639406492213097 2.0099271344700234 6 0.38681927745623024 1.4591254321570193 7 0.2179466824473536 0.9591390434017223 8 0.15155232885412007 0.7622297033656071 9 0.1327887071864671 0.7513407076032412 >10 0.4604304086139457 4.62328611743782 >50 0.02742375474503125 1.1633077139460812 >100 0.01732026615475658 2.5834142446212898 >500 0.004330066538689145 2.014464216037676 >1k 0.005773422051585526 5.159569158734336 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 2237 2.029890293367694 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1212 1.0997885719989473 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1152 1.0453435931871182 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 1085 0.9845467001805759 RNA PCR Primer, Index 39 (95% over 23bp) TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 871 0.7903596090850522 RNA PCR Primer, Index 39 (96% over 30bp) ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT 770 0.6987105614184732 RNA PCR Primer, Index 39 (96% over 25bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 579 0.5253940455341506 No Hit CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT 390 0.353892362276889 RNA PCR Primer, Index 39 (96% over 28bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 370 0.33574403600627933 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 344 0.31215121185448674 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 330 0.2994473834650599 No Hit CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA 302 0.27403972668620635 RNA PCR Primer, Index 39 (96% over 30bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 243 0.22050216418790777 No Hit CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG 228 0.20689091948495048 Illumina PCR Primer Index 11 (96% over 25bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 189 0.17150168325726162 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 126 0.11433445550484106 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 115 0.10435287605600574 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 9.074163135304846E-4 0.0 4 0.0 0.0 0.0 9.074163135304846E-4 0.0 5 0.0 0.0 0.0 0.0018148326270609693 0.0 6 0.0 0.0 0.0 0.0018148326270609693 0.0 7 0.0 0.0 0.0 0.0018148326270609693 0.0 8 0.0 0.0 0.0 0.0018148326270609693 0.0 9 0.0 0.0 0.0 0.0018148326270609693 0.0 10-11 0.0 0.0 0.0 0.0018148326270609693 0.0 12-13 0.0 0.0 0.0 0.0018148326270609693 0.0 14-15 0.0 0.0 0.0 0.0018148326270609693 0.0 16-17 0.0 0.0 0.0 0.0018148326270609693 0.0 18-19 0.0 0.0 0.0 0.0018148326270609693 0.0 20-21 0.0 0.0 0.0 0.0018148326270609693 0.0 22-23 0.0 0.0 0.0 0.0018148326270609693 0.0 24-25 0.0 0.0 0.0 0.0036296652541219386 0.0 26-27 0.0 0.0 0.0 0.005444497881182908 0.0 28-29 0.0 0.0 0.0 0.010888995762365815 0.0 30-31 0.0 0.0 0.0 0.023139115995027357 0.0 32-33 0.0 0.0 0.0 0.05126902171447238 0.0 34-35 0.0 0.0 0.0 0.10707512499659719 0.0 36-37 0.0 0.0 0.0 0.16787201800313967 0.0 38-39 0.0 0.0 0.0 0.23638194967469126 0.0 40-41 0.0 0.0 0.0 0.2853824306053374 0.0 42-43 0.0 0.0 0.0 0.338919993103636 0.0 44-45 0.0 0.0 0.0 0.40697621661842237 0.0 46-47 0.0 0.0 0.0 0.49454189087411415 0.0 48-49 0.0 0.0 0.0 0.5739408183080316 0.0 50-51 0.0 0.0 0.0 0.6374599602551655 0.0 52-53 0.0 0.0 0.0 0.7105069734943694 0.0 54-55 0.0 0.0 0.0 0.7939892743391741 0.0 56-57 0.0 0.0 0.0 0.9160367685090243 0.0 58-59 0.0 0.0 0.0 1.0340008892679873 0.0 60-61 0.0 0.0 0.0 1.1283721858751576 0.0 62-63 0.0 0.0 0.0 1.2408918087529377 0.0 64-65 0.0 0.0 0.0 1.3697449252742666 0.0 66-67 0.0 0.0 0.0 1.518107492536501 0.0 68-69 0.0 0.0 0.0 1.6927851328911192 0.0 70-71 0.0 0.0 0.0 1.8470459061913016 0.0 72-73 0.0 0.0 0.0 2.010380842626789 0.0 74-75 0.0 0.0 0.0 2.1832436503543464 0.0 76-77 0.0 0.0 0.0 2.3692639946280956 0.0 78-79 0.0 0.0 0.0 2.5997477382648384 0.0 80-81 0.0 0.0 0.0 2.829324065588051 0.0 82-83 0.0 0.0 0.0 3.0697893886736294 0.0 84-85 0.0 0.0 0.0 3.3488199050842535 0.0 86-87 0.0 0.0 0.0 3.6478135803925484 0.0 88-89 0.0 0.0 0.0 4.0012522345126715 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTACTAT 55 4.9376464E-5 43.176456 1 CGTCAGA 45 9.5930416E-4 42.21698 2 GCCTGCG 25 0.0016139675 37.99528 88-89 GGTATCA 830 0.0 37.194176 1 ACTATAC 60 0.0039379974 31.662735 3 TATACAC 705 0.0 30.989058 3 TTATACA 735 0.0 30.370377 2 GTATCAA 1220 0.0 30.365084 1 CTTATAC 725 0.0 30.134188 1 CATGGGG 270 0.0 28.144653 4 TTCCGAG 35 0.008345144 27.164137 70-71 GAGTAGT 35 0.008382197 27.139486 6 GACTTGT 35 0.008382197 27.139486 46-47 GTACATG 735 0.0 27.139484 1 TACATGG 760 0.0 26.246742 2 ACATGGG 755 0.0 25.791498 3 TATCAAC 1460 0.0 25.373562 2 TACACAT 880 0.0 25.366169 5 ATACACA 900 0.0 25.330187 4 ATCAACG 1465 0.0 25.286963 3 >>END_MODULE