FastQCFastQC Report
Fri 10 Feb 2017
SRR2031722_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031722_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3244817
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118540.3653210643312088No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113980.3512678835200876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50500.15563281380737343No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33070.10191637925960077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68550.062.094331
GTATCAA121600.041.0992161
TGGTATC28250.033.464632
TCAACGC149450.033.05744
ATCAACG149550.033.0035323
GTGGTAT29800.032.9993551
TATCAAC151850.032.6928562
CAACGCA152950.032.362565
AACGCAG158650.031.4050646
ACGCAGA180500.027.5244567
CGCAGAG181550.027.2631448
GAGTACT114150.024.17880612-13
GCAGAGT197500.024.0031329
CAGAGTA187750.022.80921710-11
GTACTTT125750.021.62745914-15
GTACATG113050.020.0446821
TACATGG114050.019.4523892
AGAGTAC178000.019.0947610-11
ACATGGG117200.018.1991043
AGTACTT126200.018.04944612-13
Produced by FastQC (version 0.11.5)