##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031722_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3244817
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.904418954905623	33.0	31.0	34.0	30.0	34.0
2	32.216223595968586	34.0	31.0	34.0	31.0	34.0
3	32.313977953148054	34.0	31.0	34.0	31.0	34.0
4	35.76125833906812	37.0	35.0	37.0	35.0	37.0
5	35.76111657452485	37.0	35.0	37.0	35.0	37.0
6	35.79401426952583	37.0	37.0	37.0	35.0	37.0
7	35.77461872271996	37.0	37.0	37.0	35.0	37.0
8	35.76623581545585	37.0	37.0	37.0	35.0	37.0
9	37.52622628641307	39.0	38.0	39.0	35.0	39.0
10-11	37.45242458973803	39.0	38.0	39.0	35.0	39.0
12-13	37.39630570845752	39.0	38.0	39.0	35.0	39.0
14-15	38.75323215454061	41.0	39.0	41.0	35.0	41.0
16-17	38.67444173276952	41.0	38.5	41.0	35.0	41.0
18-19	38.70153971703181	41.0	39.0	41.0	35.0	41.0
20-21	38.71268502969505	41.0	39.0	41.0	35.0	41.0
22-23	38.61144156974029	41.0	39.0	41.0	34.5	41.0
24-25	38.53164939656073	40.5	38.5	41.0	34.0	41.0
26-27	38.407747648018365	40.0	38.0	41.0	34.0	41.0
28-29	38.290123140996855	40.0	38.0	41.0	34.0	41.0
30-31	38.10530470593565	40.0	38.0	41.0	34.0	41.0
32-33	37.872494042036884	40.0	38.0	41.0	33.0	41.0
34-35	37.69124976847692	40.0	38.0	41.0	33.0	41.0
36-37	37.51424625795538	40.0	37.5	41.0	33.0	41.0
38-39	37.316264985051546	40.0	37.0	41.0	32.0	41.0
40-41	37.19859086043989	40.0	37.0	41.0	31.5	41.0
42-43	37.13244521956092	40.0	37.0	41.0	31.0	41.0
44-45	36.95027377506959	40.0	37.0	41.0	31.0	41.0
46-47	36.80351264801682	40.0	37.0	41.0	31.0	41.0
48-49	36.779723170829044	40.0	37.0	41.0	30.5	41.0
50-51	36.2022383696831	39.5	36.0	40.5	29.5	41.0
52-53	36.20438980071911	39.5	36.0	40.5	30.0	41.0
54-55	36.41888556427065	40.0	36.5	41.0	30.0	41.0
56-57	36.27929325444239	40.0	36.0	41.0	30.0	41.0
58-59	36.07693900765436	39.5	35.5	41.0	29.0	41.0
60-61	35.8258602873444	39.0	35.0	41.0	28.5	41.0
62-63	35.526088528259066	39.0	35.0	41.0	28.0	41.0
64-65	35.23808476718409	38.5	35.0	41.0	28.0	41.0
66-67	34.93296601934716	37.5	35.0	40.5	28.0	41.0
68-69	34.58026354028594	37.0	35.0	40.0	26.5	41.0
70-71	34.20480215062976	36.5	34.5	39.0	26.0	41.0
72-73	33.82699763962036	36.0	34.0	39.0	26.0	41.0
74-75	33.41920391812543	35.5	34.0	39.0	26.0	40.5
76-77	33.03993245227697	35.0	34.0	37.5	25.5	39.5
78-79	32.65426802189461	35.0	34.0	37.0	24.5	39.0
80-81	32.31073924970191	35.0	34.0	36.5	24.0	39.0
82-83	31.99497336829781	35.0	33.0	36.0	23.5	37.5
84-85	31.683593096313288	35.0	33.0	36.0	22.5	37.0
86-87	31.415034345542445	35.0	33.0	35.0	20.5	37.0
88-89	31.14703957110678	35.0	33.0	35.0	19.5	36.0
90-91	30.912500150239598	35.0	33.0	35.0	18.0	36.0
92-93	30.696182250031356	35.0	32.0	35.0	15.5	36.0
94-95	30.446111907081352	35.0	32.0	35.0	6.0	36.0
96-97	30.17528045495324	35.0	32.0	35.0	2.0	35.0
98-99	29.912880911311795	35.0	32.0	35.0	2.0	35.0
100-101	29.324815082021573	34.5	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.06708310753835534
1101	2	0.092828828131168
1101	3	0.18351905795255163
1101	4	0.2780713925064191
1101	5	0.1526193167388783
1101	6	0.2851876606556871
1101	7	0.25602116810762965
1101	8	0.270196060034813
1101	9	0.2976629212977855
1101	10-11	0.17078681554781383
1101	12-13	0.16515430483030968
1101	14-15	0.1653613083128178
1101	16-17	0.31684688212667567
1101	18-19	0.2912251618278674
1101	20-21	0.24637538426279804
1101	22-23	0.19768438236224029
1101	24-25	0.08246432763365164
1101	26-27	0.04540091049728545
1101	28-29	0.125780884834235
1101	30-31	0.1378652912715168
1101	32-33	0.0475348458341287
1101	34-35	0.14383491248110403
1101	36-37	-0.1348801395074446
1101	38-39	0.12969543771185243
1101	40-41	-0.1327974708265458
1101	42-43	-0.060262335265839795
1101	44-45	-0.19553254056719993
1101	46-47	-0.10872725612605905
1101	48-49	-0.0018117038563119081
1101	50-51	0.03369502250242107
1101	52-53	0.021261185496776136
1101	54-55	0.12876916297614116
1101	56-57	-0.016244804272751878
1101	58-59	-0.18771801856728132
1101	60-61	-0.4537054840246526
1101	62-63	-0.22698566118965857
1101	64-65	-0.03753664977065796
1101	66-67	-0.3064531138329869
1101	68-69	-0.4679236171922341
1101	70-71	-0.3942095488391857
1101	72-73	-0.021012787484032458
1101	74-75	0.09091364017620407
1101	76-77	-0.24029429724780016
1101	78-79	-0.4166413195476437
1101	80-81	-0.48458447140230376
1101	82-83	-0.3970998681642044
1101	84-85	-0.4422540233659582
1101	86-87	-0.43089791748512596
1101	88-89	-0.5929447783631794
1101	90-91	-0.8789637820762266
1101	92-93	-0.6785709772518587
1101	94-95	-0.7200652733274815
1101	96-97	-0.8003579237780976
1101	98-99	-0.7607860974693814
1101	100-101	-0.7886115182856734
1102	1	0.10691568183153422
1102	2	0.16001786960408282
1102	3	0.2505788539678875
1102	4	0.2753646764847275
1102	5	0.22838318993699858
1102	6	0.20018971091636217
1102	7	0.24422821747102574
1102	8	0.05356021701503977
1102	9	0.23343722711485526
1102	10-11	0.2919311864432217
1102	12-13	0.3583432551805288
1102	14-15	0.5493393347253885
1102	16-17	0.4448022096895343
1102	18-19	0.32730463569657076
1102	20-21	0.3072723105454642
1102	22-23	0.37465628216818203
1102	24-25	0.18823918204655143
1102	26-27	0.24862591010210622
1102	28-29	0.278686164650658
1102	30-31	0.13412693614777282
1102	32-33	0.29601993100131097
1102	34-35	0.34922407613282047
1102	36-37	0.2792911135133167
1102	38-39	0.10582919467275076
1102	40-41	0.2541662186384599
1102	42-43	0.28567150035811295
1102	44-45	0.12682190254759718
1102	46-47	0.2562251880543158
1102	48-49	0.3559969068665936
1102	50-51	0.3714464118910925
1102	52-53	0.3843874564491827
1102	54-55	0.3938502017376919
1102	56-57	0.40715262179001144
1102	58-59	0.28405440156785744
1102	60-61	0.20757750494856708
1102	62-63	0.14988897908490628
1102	64-65	0.16622113866289112
1102	66-67	0.1622239240842802
1102	68-69	0.13569683631985185
1102	70-71	0.10407306727483956
1102	72-73	0.16871164074429856
1102	74-75	-0.08352030656165255
1102	76-77	0.16466984411139407
1102	78-79	0.012612134513965145
1102	80-81	0.10448564007423045
1102	82-83	0.1965686851142152
1102	84-85	0.2483328160990972
1102	86-87	0.18733657096786516
1102	88-89	0.16255861409762318
1102	90-91	0.2149483849423781
1102	92-93	0.3295496357249448
1102	94-95	-0.022528468591332285
1102	96-97	-0.24672849746985648
1102	98-99	0.026389870686024608
1102	100-101	-0.044189761533061755
1103	1	-0.0943924514505845
1103	2	0.1093898927493413
1103	3	0.11014899753509155
1103	4	0.0904058052235257
1103	5	0.08502816488219622
1103	6	0.07389945584378665
1103	7	0.06956696715533894
1103	8	0.15529659225044412
1103	9	0.20071580175720527
1103	10-11	0.1430423304512516
1103	12-13	0.21937990538355479
1103	14-15	0.394063921729888
1103	16-17	0.4013448752204951
1103	18-19	0.3206518034490884
1103	20-21	0.15476093453825257
1103	22-23	0.17915496097735684
1103	24-25	0.23352431987704136
1103	26-27	0.34672654329185804
1103	28-29	0.1386511344943031
1103	30-31	0.12101276967216279
1103	32-33	0.12219280734078097
1103	34-35	0.2016374319777654
1103	36-37	0.13851527007430775
1103	38-39	0.2459990389286162
1103	40-41	0.23137890328506927
1103	42-43	0.3084576137087538
1103	44-45	0.12938008074665674
1103	46-47	0.3221793327875915
1103	48-49	0.32614573758360876
1103	50-51	0.2654360311421655
1103	52-53	0.2538800695728298
1103	54-55	0.11935901583417774
1103	56-57	0.253352319239589
1103	58-59	0.2151351058048263
1103	60-61	0.28853498492320995
1103	62-63	0.27347985226526816
1103	64-65	0.2334488369566543
1103	66-67	0.19115448577638006
1103	68-69	0.1750579351578878
1103	70-71	0.20535192230014587
1103	72-73	0.05657142547698868
1103	74-75	0.01239489581259079
1103	76-77	0.013916934885124022
1103	78-79	-0.014348914017084269
1103	80-81	0.010786677744235362
1103	82-83	-0.010239496501064593
1103	84-85	-0.07689079437305324
1103	86-87	0.011499319105517714
1103	88-89	-0.028594114144990357
1103	90-91	-0.01249021296062125
1103	92-93	-0.5404402269633195
1103	94-95	-0.04929776604981839
1103	96-97	-0.03646961897133494
1103	98-99	-0.008508761257694175
1103	100-101	0.054986474340875446
1104	1	-0.03861143127686617
1104	2	0.015265002919548465
1104	3	0.17222301825245978
1104	4	0.01326372054447944
1104	5	0.03334088918776956
1104	6	0.0742864546364288
1104	7	0.10358406342482596
1104	8	0.0606324418079609
1104	9	0.17517558950812884
1104	10-11	0.200700148363687
1104	12-13	0.2500327230568544
1104	14-15	0.25613142739822337
1104	16-17	0.29542921070013506
1104	18-19	0.22806624645875928
1104	20-21	0.11697731029699554
1104	22-23	0.24242389536910736
1104	24-25	0.19632900212322113
1104	26-27	0.06496506924127488
1104	28-29	0.05498338637829647
1104	30-31	0.19748273065995647
1104	32-33	0.10209729721000116
1104	34-35	0.19672393816437506
1104	36-37	0.20022876701101922
1104	38-39	0.17424980438588022
1104	40-41	0.2933370195720997
1104	42-43	0.1754423953350468
1104	44-45	0.19390871328911174
1104	46-47	0.1725684300604513
1104	48-49	0.3065608169696006
1104	50-51	0.22759421067642194
1104	52-53	0.19461118007880174
1104	54-55	0.28424014425043254
1104	56-57	0.20492110400481778
1104	58-59	0.12152214754311075
1104	60-61	0.1707122752679524
1104	62-63	0.20977645926677013
1104	64-65	0.218391875419357
1104	66-67	0.2234564404862951
1104	68-69	0.25004651552138313
1104	70-71	0.2342594632619921
1104	72-73	0.20522764171219876
1104	74-75	0.2505939947349489
1104	76-77	0.2535165253398688
1104	78-79	0.11789023269206211
1104	80-81	0.07154951434290524
1104	82-83	0.12701904406938525
1104	84-85	0.18010080487383462
1104	86-87	0.19417019151694603
1104	88-89	0.35926895953314286
1104	90-91	0.36082441461770287
1104	92-93	0.1622519767434767
1104	94-95	0.37245320095914636
1104	96-97	0.2486916370325183
1104	98-99	0.03935135014720714
1104	100-101	0.15993073773039868
1105	1	-0.03999748265351499
1105	2	0.17366610670173088
1105	3	0.10321063489753612
1105	4	0.1412478121035008
1105	5	0.23075027531784542
1105	6	0.2520592541627664
1105	7	0.2257514468113726
1105	8	0.2929342309571794
1105	9	0.17959371609973118
1105	10-11	0.2639920250674166
1105	12-13	0.21272439126735065
1105	14-15	0.3171188590178602
1105	16-17	0.3231113594685553
1105	18-19	0.3138001288102146
1105	20-21	0.35179011724308396
1105	22-23	0.13137343799323276
1105	24-25	0.23552769673356977
1105	26-27	0.23111335238079533
1105	28-29	0.13402368047038493
1105	30-31	0.2490567585210357
1105	32-33	0.3628724531173617
1105	34-35	0.4189481056259936
1105	36-37	0.4262760520202278
1105	38-39	0.3864508038511758
1105	40-41	0.39502911240428773
1105	42-43	0.3218452430405989
1105	44-45	0.5193174731431753
1105	46-47	0.5039940300405874
1105	48-49	0.45828150677257184
1105	50-51	0.5130943871532239
1105	52-53	0.641185433951982
1105	54-55	0.5542871828713345
1105	56-57	0.5557230253652605
1105	58-59	0.4039656568153447
1105	60-61	0.5234565539415641
1105	62-63	0.596147239017867
1105	64-65	0.44430460711396336
1105	66-67	0.5158273261000517
1105	68-69	0.6109065020824431
1105	70-71	0.5689154552876303
1105	72-73	0.3387963984559619
1105	74-75	0.4093457685785822
1105	76-77	0.5291450673182823
1105	78-79	0.49628196575515204
1105	80-81	0.3960700935053296
1105	82-83	0.41118539787129293
1105	84-85	0.4502138905525648
1105	86-87	0.41788808813831224
1105	88-89	0.4598355004215371
1105	90-91	0.29600080827503206
1105	92-93	0.2748067027032235
1105	94-95	0.32054759110473796
1105	96-97	0.36878140716299157
1105	98-99	0.419666879426547
1105	100-101	0.37271632392028664
1106	1	-0.0703962415945405
1106	2	0.11554030064411336
1106	3	0.07042804781105616
1106	4	0.04504738721922763
1106	5	0.07190567221354627
1106	6	0.13572432486223107
1106	7	0.1820212707951967
1106	8	0.15591939782848385
1106	9	0.26893120765399203
1106	10-11	0.1665588618019953
1106	12-13	0.1749524507600526
1106	14-15	0.1746811783814124
1106	16-17	0.20136309350052528
1106	18-19	0.07587333402567253
1106	20-21	0.1557622445855671
1106	22-23	0.21959838371469687
1106	24-25	0.3403191356869897
1106	26-27	0.25157728783077715
1106	28-29	0.18787583774030736
1106	30-31	0.1933371394262977
1106	32-33	0.2368677246168147
1106	34-35	0.15341612484166944
1106	36-37	0.10828833891344658
1106	38-39	0.1901630238459191
1106	40-41	0.09486488077227762
1106	42-43	0.14736687647568658
1106	44-45	0.07709187868228895
1106	46-47	0.1419245179233357
1106	48-49	0.18797213638129762
1106	50-51	0.07746614554184106
1106	52-53	0.13470910793505908
1106	54-55	0.1392789434802566
1106	56-57	-0.03567003129866464
1106	58-59	0.15516057431131003
1106	60-61	-0.010352703320769763
1106	62-63	0.07424916897313949
1106	64-65	0.16687968212723803
1106	66-67	0.16477715135643933
1106	68-69	0.11163850306756018
1106	70-71	0.052437507422162355
1106	72-73	-0.0768708698594125
1106	74-75	-0.05749666138214593
1106	76-77	-0.020812762394740503
1106	78-79	0.02786140374124102
1106	80-81	0.0684967882870069
1106	82-83	0.08102469915491639
1106	84-85	0.07101591134470553
1106	86-87	0.026261109299539953
1106	88-89	0.04477664778706014
1106	90-91	0.08390523311836873
1106	92-93	0.2183912349400785
1106	94-95	0.32164206077917257
1106	96-97	0.39107609593159154
1106	98-99	0.4135954593568698
1106	100-101	0.4342702038870314
1107	1	0.08819551353316868
1107	2	0.2505354987438082
1107	3	0.26424534071371397
1107	4	0.22783972539424013
1107	5	0.2136367904217451
1107	6	0.26445337455953677
1107	7	0.3080893857983398
1107	8	0.26110161431746803
1107	9	0.2690235518905766
1107	10-11	0.25641394764898706
1107	12-13	0.2707213562298634
1107	14-15	0.3269254511445041
1107	16-17	0.3813049545162883
1107	18-19	0.26791524978499837
1107	20-21	0.27017178133004194
1107	22-23	0.3968986076139558
1107	24-25	0.3413473532443234
1107	26-27	0.3739988709993156
1107	28-29	0.41876519797943956
1107	30-31	0.36101971108542585
1107	32-33	0.39875356440950327
1107	34-35	0.3657957931979965
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2314	94-95	0.0018815040936921434
2314	96-97	2.316346390784929E-4
2314	98-99	0.06030357344458537
2314	100-101	0.14515293786596217
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2315	3	-0.34208478749141236
2315	4	-0.8324415522361761
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2316	1	-0.33264169136737465
2316	2	-0.455335087669571
2316	3	-0.6351769685139814
2316	4	-0.4792964002324922
2316	5	-0.5343696833680696
2316	6	-1.0651538235111673
2316	7	-0.7617148055537015
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2316	9	-0.6981127987503157
2316	10-11	-1.122367696622156
2316	12-13	-0.7914600667332294
2316	14-15	-1.0211348109572853
2316	16-17	-0.9294929622496468
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2316	54-55	-0.7088020103769566
2316	56-57	-0.6055047799343427
2316	58-59	-0.6034013040110509
2316	60-61	-0.7241043744625344
2316	62-63	-0.6498127219501555
2316	64-65	-0.6763941677188399
2316	66-67	-0.5318560008777027
2316	68-69	-0.6912515588045309
2316	70-71	-0.6681803056814104
2316	72-73	-0.5912626532010989
2316	74-75	-0.47833987936279954
2316	76-77	-0.459470435133035
2316	78-79	-0.4440526354285268
2316	80-81	-0.5249141536342279
2316	82-83	-0.4113430829017801
2316	84-85	-0.3529618266026553
2316	86-87	-0.32298596219815323
2316	88-89	-0.2357238339329335
2316	90-91	-0.3379081048626347
2316	92-93	-0.2850699224571116
2316	94-95	-0.3036223690346951
2316	96-97	-0.28481842606341345
2316	98-99	-0.31030155671189874
2316	100-101	-0.3781321958163737
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	17050.0
3	2722.0
4	2132.0
5	2216.0
6	2514.0
7	3068.0
8	3807.0
9	4432.0
10	5358.0
11	6408.0
12	7898.0
13	10061.0
14	11934.0
15	14463.0
16	17654.0
17	25174.0
18	24180.0
19	14565.0
20	12698.0
21	12469.0
22	12891.0
23	14149.0
24	15858.0
25	18143.0
26	21062.0
27	24614.0
28	29507.0
29	35588.0
30	43560.0
31	54441.0
32	69199.0
33	91975.0
34	131163.0
35	206068.0
36	355954.0
37	690802.0
38	945247.0
39	283403.0
40	390.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.9111836927193	19.47835690109319	14.0563626161232	23.554096790064317
2	16.58848683834848	21.566119392435166	38.46867338343386	23.376720385782495
3	18.69408680058828	25.981410235635988	30.709222813279812	24.615280150495916
4	13.889513925349348	18.930085736482777	36.77399700167695	30.40640333649092
5	14.28595236750517	36.599506879704016	34.324913129906555	14.789627622884263
6	29.70185733227064	36.30911331075271	18.801011222121733	15.18801813485492
7	25.59548606289518	33.182591039396655	23.15358433391038	18.06833856379778
8	24.671310736663212	33.69219609260041	21.68560795981708	19.950885210919292
9	25.961683237233917	16.465057412819398	21.6117242111291	35.96153513881758
10-11	24.02525645787649	26.45852126258843	28.70818098466497	20.8080412948701
12-13	25.28685641959311	25.186420224116198	28.00844589721227	21.518277459078423
14-15	22.887707977907866	26.675396451135843	26.598874530591488	23.83802104036481
16-17	21.679948887626853	28.92543643052915	27.522855502605005	21.871759179238996
18-19	21.46325933518487	27.55738517756176	29.808409748909998	21.170945738343374
20-21	22.669983833364373	26.67786516467301	29.506432321173666	21.145718680788956
22-23	22.467127768206215	26.821130436312163	29.073544492039964	21.638197303441657
24-25	22.002240951844527	27.102944168889337	29.473036918624878	21.421777960641258
26-27	21.68687542995659	27.154989872347922	29.74516639696107	21.412968300734413
28-29	21.685229956969373	27.06103437050157	29.785433152869047	21.468302519660014
30-31	21.996562836986218	27.02091455070818	29.52390355579759	21.458619056508006
32-33	21.878973851392555	27.108541072123604	29.30555107749368	21.70693399899016
34-35	22.083456640324485	27.2981620135057	29.197850863292945	21.420530482876863
36-37	21.93965170071554	27.2436968618008	29.30995908333588	21.506692354147784
38-39	21.742772709355577	27.38145176033713	29.42541911227573	21.450356418031564
40-41	21.950076700994792	27.200179953817404	29.36567359333554	21.484069751852264
42-43	21.905327570739743	27.31868926965066	29.05957774063238	21.71640541897722
44-45	22.021882628137277	27.47052125154684	28.848778109348334	21.658818010967547
46-47	22.022254994628966	27.53599641540229	28.63686537792726	21.804883212041485
48-49	21.9052060428559	27.64455190371997	28.670279425837027	21.7799626275871
50-51	22.004190698377723	27.826977852251648	28.368620988671537	21.800210460699088
52-53	22.160797895727434	27.931626568755252	28.07306330559005	21.83451222992726
54-55	22.15449114044645	27.905677034268173	28.078676926442114	21.861154898843267
56-57	22.002678792256898	28.077189702834886	28.08137097631501	21.8387605285932
58-59	21.960470378856648	28.001207666118887	28.261763019745374	21.77655893527909
60-61	22.053996614643918	27.977536687546937	28.290463058211955	21.678003639597186
62-63	21.9659334064588	27.947272528120138	28.27372990785266	21.81306415756841
64-65	22.00670370234352	27.916112062497938	28.1644996341387	21.912684601019844
66-67	21.924054375027097	28.099668314371385	28.026116405192486	21.950160905409035
68-69	21.821972014408637	28.11143934384998	28.17129274237039	21.895295899370993
70-71	21.930592450265042	27.938160267018098	28.16675918414702	21.96448809856984
72-73	21.906536173088362	27.985441742670925	28.045566296307438	22.062455787933278
74-75	21.971222475580383	28.163088886756714	27.860637654260618	22.00505098340228
76-77	21.941514209319468	28.125890584976716	27.891867804825587	22.04072740087823
78-79	21.823358930827208	28.126949456509077	27.922720863055595	22.12697074960812
80-81	21.784941083310407	28.275906656003592	27.832961035999304	22.1061912246867
82-83	21.86983791964603	28.287332358004747	27.8316303659982	22.011199356351028
84-85	21.91523975984516	28.17583972725836	27.711940716712093	22.196979796184383
86-87	21.846474805823256	28.184934492594394	27.85014886819282	22.118441833389525
88-89	21.83336496196079	28.16822418371141	27.839348172559617	22.15906268176818
90-91	21.828809420428723	28.13975973067913	27.905517356093156	22.125913492798986
92-93	21.82536277688203	28.11131947822351	27.972122291056074	22.091195453838385
94-95	21.778076264761452	28.067501463378207	27.952082039281173	22.202340232579164
96-97	21.858708200901003	28.064238858869867	27.84127125164827	22.235781688580865
98-99	21.68937229658611	28.0674814684203	27.90076628512076	22.342379949872832
100-101	21.71537707675806	28.057967883076497	27.899497642546823	22.327157397618624
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6446.0
1	3708.0
2	921.0
3	818.5
4	671.0
5	665.5
6	921.0
7	1643.5
8	2719.0
9	3324.0
10	2956.0
11	2442.5
12	2571.0
13	2884.5
14	3114.0
15	3416.5
16	3716.5
17	4045.0
18	4412.0
19	4679.5
20	5153.0
21	5995.0
22	7026.5
23	8457.0
24	10399.5
25	13275.0
26	17621.5
27	23445.5
28	29474.0
29	36217.5
30	44894.5
31	53023.0
32	60624.0
33	68333.5
34	75606.0
35	81805.0
36	89780.0
37	98155.0
38	102546.0
39	105513.5
40	107719.5
41	109677.0
42	111409.5
43	113724.0
44	117235.5
45	121152.0
46	123715.0
47	123071.5
48	121542.5
49	119710.5
50	116053.5
51	111877.5
52	107271.5
53	102336.0
54	96715.5
55	88100.0
56	78643.5
57	69919.5
58	62556.0
59	54950.5
60	45859.0
61	37549.5
62	29547.5
63	21822.0
64	15917.5
65	11713.5
66	8597.5
67	6529.5
68	5023.0
69	3820.0
70	2851.0
71	2119.0
72	1591.5
73	1129.5
74	803.5
75	622.5
76	458.0
77	312.5
78	218.0
79	155.5
80	105.0
81	83.0
82	77.5
83	68.0
84	49.5
85	37.0
86	30.0
87	26.0
88	26.5
89	19.5
90	16.0
91	14.0
92	12.5
93	10.0
94	7.0
95	7.5
96	4.0
97	3.5
98	2.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15221197374150838
2	0.15606427111297802
3	0.14983895856068308
4	0.1542768051326161
5	0.14281236815512247
6	0.12284205858142386
7	0.129036552754747
8	0.11828093849360381
9	0.11492173518568227
10-11	0.10321075117641458
12-13	0.12184046126484174
14-15	0.14080917352195824
16-17	0.15091760182469457
18-19	0.1480360833908353
20-21	0.13872893294136465
22-23	0.13301212364210369
24-25	0.11660133683964304
26-27	0.12134736720129363
28-29	0.1264169905421477
30-31	0.11624692548146782
32-33	0.1142591400377895
34-35	0.1312862944196853
36-37	0.1333819441897648
38-39	0.1198680850106493
40-41	0.12282664939193798
42-43	0.11709443090319116
44-45	0.13396749339022818
46-47	0.13208757227295098
48-49	0.14566306821001
50-51	0.13273475823135789
52-53	0.11686329306090297
54-55	0.10741745990605941
56-57	0.12874377815451535
58-59	0.11880485093612367
60-61	0.11707902171370528
62-63	0.11747966064033812
64-65	0.13923743619439863
66-67	0.13093188306151007
68-69	0.1355854582862454
70-71	0.12290369533936736
72-73	0.12464493375127166
74-75	0.10687813827405368
76-77	0.1331508063474766
78-79	0.13345899013719417
80-81	0.13410617609560108
82-83	0.13996166810023494
84-85	0.13070074521922193
86-87	0.13974593944743263
88-89	0.12580062296271255
90-91	0.1346763161065786
92-93	0.14820558447517995
94-95	0.12413643049823767
96-97	0.1090662431810484
98-99	0.11281067622611692
100-101	0.15692718572418723
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3244817.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.66151402262726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.46303458297272	27.746352220213517
2	19.950820392546447	18.61870971019448
3	8.784929067382526	12.297542725963668
4	4.437600166157139	8.282605695198177
5	2.4012145796479563	5.602215388979006
6	1.4705426716706067	4.117064849701786
7	0.8812490080368597	2.87842890621568
8	0.6068287730073967	2.265243944081467
9	0.4055431589381039	1.70309320178138
>10	1.5143621491889119	11.503879701530256
>50	0.059604711913140956	1.9048457903002962
>100	0.023344833972555767	1.7816418140210337
>500	4.629522829043272E-4	0.1514921217787671
>1k	2.645441615936719E-4	0.2734820091491606
>5k	6.613604039841797E-5	0.1558433927108171
>10k+	1.3227208079683595E-4	0.717558528180578
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11854	0.3653210643312088	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11398	0.3512678835200876	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5050	0.15563281380737343	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3307	0.10191637925960077	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	3.0818378971757115E-5	0.0
12-13	0.0	0.0	0.0	3.0818378971757115E-5	0.0
14-15	0.0	0.0	0.0	6.163675794351423E-5	0.0
16-17	0.0	0.0	0.0	6.163675794351423E-5	0.0
18-19	0.0	0.0	0.0	6.163675794351423E-5	0.0
20-21	0.0	0.0	0.0	1.6950108434466414E-4	0.0
22-23	0.0	0.0	0.0	4.4686649509047814E-4	0.0
24-25	0.0	0.0	0.0	6.780043373786565E-4	0.0
26-27	0.0	0.0	0.0	0.0012327351588702845	0.0
28-29	0.0	0.0	0.0	0.002434651938768812	0.0
30-31	0.0	0.0	0.0	0.005963356331035001	0.0
32-33	0.0	0.0	0.0	0.013806633779347186	0.0
34-35	0.0	0.0	0.0	0.026488396726225237	0.0
36-37	0.0	0.0	0.0	0.04373127976092334	0.0
38-39	0.0	0.0	0.0	0.0623763990388364	0.0
40-41	0.0	0.0	0.0	0.07877177665181118	0.0
42-43	0.0	0.0	0.0	0.0999594121948942	0.0
44-45	0.0	0.0	0.0	0.12832773003839662	0.0
46-47	0.0	0.0	0.0	0.15886874359940792	0.0
48-49	0.0	0.0	0.0	0.18537254951511906	0.0
50-51	0.0	0.0	0.0	0.21001184350303886	0.0
52-53	0.0	0.0	0.0	0.23881161865214587	0.0
54-55	0.0	0.0	0.0	0.2758244917972261	0.0
56-57	0.0	0.0	0.0	0.3173060298932112	0.0
58-59	0.0	0.0	0.0	0.358448565820507	0.0
60-61	0.0	0.0	0.0	0.3988822790314523	0.0
62-63	0.0	0.0	0.0	0.44551048641572083	0.0
64-65	0.0	0.0	0.0	0.5005212928803073	0.0
66-67	0.0	0.0	0.0	0.563991744372641	0.0
68-69	0.0	0.0	0.0	0.631622677026162	0.0
70-71	0.0	0.0	0.0	0.6991919729217395	0.0
72-73	0.0	0.0	0.0	0.7663606298906842	0.0
74-75	0.0	0.0	0.0	0.8473975573969195	0.0
76-77	0.0	0.0	0.0	0.9387740510481792	0.0
78-79	0.0	0.0	0.0	1.0358365356197283	0.0
80-81	0.0	0.0	0.0	1.137783733258301	0.0
82-83	0.0	0.0	0.0	1.247527980776728	0.0
84-85	0.0	0.0	0.0	1.3862260953391208	0.0
86-87	0.0	0.0	0.0	1.5564360024001354	0.0
88-89	0.0	0.0	0.0	1.7437038822220174	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6855	0.0	62.09433	1
GTATCAA	12160	0.0	41.099216	1
TGGTATC	2825	0.0	33.46463	2
TCAACGC	14945	0.0	33.0574	4
ATCAACG	14955	0.0	33.003532	3
GTGGTAT	2980	0.0	32.999355	1
TATCAAC	15185	0.0	32.692856	2
CAACGCA	15295	0.0	32.36256	5
AACGCAG	15865	0.0	31.405064	6
ACGCAGA	18050	0.0	27.524456	7
CGCAGAG	18155	0.0	27.263144	8
GAGTACT	11415	0.0	24.178806	12-13
GCAGAGT	19750	0.0	24.003132	9
CAGAGTA	18775	0.0	22.809217	10-11
GTACTTT	12575	0.0	21.627459	14-15
GTACATG	11305	0.0	20.044682	1
TACATGG	11405	0.0	19.452389	2
AGAGTAC	17800	0.0	19.09476	10-11
ACATGGG	11720	0.0	18.199104	3
AGTACTT	12620	0.0	18.049446	12-13
>>END_MODULE