FastQCFastQC Report
Fri 10 Feb 2017
SRR2031722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3244817
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155450.47907170111596437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153060.47170610854171435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71170.21933440314199537No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT51080.15742027978773535No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45280.13954561998411621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70800.064.9630661
GTATCAA128600.041.528841
GTGGTAT27450.033.9264341
TCAACGC161600.032.9839254
ATCAACG162450.032.899583
TATCAAC165600.032.3311462
CAACGCA166200.032.0355155
TGGTATC28400.031.7828452
AACGCAG170800.031.2561576
ACGCAGA193100.027.523587
CGCAGAG196200.027.0644938
GCAGAGT212750.024.4456569
GAGTACT139900.023.84985412-13
CAGAGTA202750.023.26191710-11
GTACATG106750.022.7445561
TACATGG107100.021.6465072
GTACTTT156150.021.20058814-15
ACATGGG106550.020.5989953
AGAGTAC195300.020.13655510-11
CATGGGG60800.019.3775524
Produced by FastQC (version 0.11.5)