##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031722_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3244817
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71221212166973	34.0	31.0	34.0	31.0	34.0
2	32.874124796560174	34.0	31.0	34.0	31.0	34.0
3	32.918393240666575	34.0	31.0	34.0	31.0	34.0
4	36.33278363618041	37.0	37.0	37.0	35.0	37.0
5	36.30101296929842	37.0	37.0	37.0	35.0	37.0
6	36.33418124966678	37.0	37.0	37.0	35.0	37.0
7	36.28144299046757	37.0	37.0	37.0	35.0	37.0
8	36.27454460451853	37.0	37.0	37.0	35.0	37.0
9	38.11656589570383	39.0	39.0	39.0	37.0	39.0
10-11	38.08111936666998	39.0	39.0	39.0	36.0	39.0
12-13	38.024343899825475	39.0	39.0	39.0	35.0	39.0
14-15	39.47436958694435	41.0	39.0	41.0	36.0	41.0
16-17	39.443933664055635	41.0	39.0	41.0	36.0	41.0
18-19	39.480964720044305	41.0	39.0	41.0	36.5	41.0
20-21	39.48986553016703	41.0	39.0	41.0	36.5	41.0
22-23	39.39842462610372	41.0	39.0	41.0	36.0	41.0
24-25	39.32473125603077	41.0	39.0	41.0	36.0	41.0
26-27	39.18285437976934	41.0	39.0	41.0	36.0	41.0
28-29	39.074563064727535	40.5	39.0	41.0	35.0	41.0
30-31	38.884818774063376	40.0	38.0	41.0	35.0	41.0
32-33	38.645326685603536	40.0	38.0	41.0	35.0	41.0
34-35	38.481174747297	40.0	38.0	41.0	35.0	41.0
36-37	38.365122902154425	40.0	38.0	41.0	34.5	41.0
38-39	38.422832319973665	40.0	38.0	41.0	35.0	41.0
40-41	38.32810756353902	40.0	38.0	41.0	34.0	41.0
42-43	38.197798674008425	40.0	38.0	41.0	34.0	41.0
44-45	37.96192204367766	40.0	38.0	41.0	33.5	41.0
46-47	37.66791609511415	40.0	38.0	41.0	33.0	41.0
48-49	37.342034697180154	40.0	37.5	41.0	32.5	41.0
50-51	37.14837015461889	40.0	37.0	41.0	32.0	41.0
52-53	37.188060528529036	40.0	37.0	41.0	32.0	41.0
54-55	37.10189419002674	40.0	37.0	41.0	32.0	41.0
56-57	36.91015456341606	40.0	37.0	41.0	32.0	41.0
58-59	36.71866872615621	40.0	36.0	41.0	31.0	41.0
60-61	36.45678970493559	39.0	36.0	41.0	31.0	41.0
62-63	36.17918899586633	39.0	35.0	41.0	31.0	41.0
64-65	35.85525408674819	39.0	35.0	41.0	30.0	41.0
66-67	35.50709223355277	38.0	35.0	40.5	30.0	41.0
68-69	35.11661967377513	37.0	35.0	40.0	29.0	41.0
70-71	34.759000738716544	37.0	35.0	39.5	29.0	41.0
72-73	34.37077853697142	36.0	35.0	39.0	29.0	41.0
74-75	33.983367474960836	36.0	34.0	39.0	28.0	40.5
76-77	33.060517742603054	35.0	33.5	37.0	26.5	39.0
78-79	33.25310626146251	35.0	34.0	37.0	28.0	39.0
80-81	33.02967933168496	35.0	34.0	37.0	28.0	39.0
82-83	32.76033779408823	35.0	34.0	36.0	27.0	38.0
84-85	32.47013051891678	35.0	34.0	36.0	27.0	37.0
86-87	32.21943841517103	35.0	34.0	35.5	26.0	37.0
88-89	31.99255674511074	35.0	34.0	35.0	26.0	36.0
90-91	31.791205328374453	35.0	33.5	35.0	25.5	36.0
92-93	31.620080423641767	35.0	33.0	35.0	25.0	36.0
94-95	31.441165403164494	35.0	33.0	35.0	25.0	36.0
96-97	31.267691059310895	35.0	33.0	35.0	24.0	35.0
98-99	31.055488029062964	35.0	33.0	35.0	21.5	35.0
100-101	30.340766829069253	34.5	32.0	35.0	10.5	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18476430142948175
1101	2	0.1702972508542402
1101	3	0.17351561768212065
1101	4	0.120831652872468
1101	5	0.1208572484667556
1101	6	0.0932646837611415
1101	7	0.15915519236354925
1101	8	0.16893671384205788
1101	9	0.07232416633373617
1101	10-11	0.16995882307053733
1101	12-13	0.21627714061557413
1101	14-15	0.2585634532326111
1101	16-17	0.20410330662495824
1101	18-19	0.2117089633612963
1101	20-21	0.1707355300265121
1101	22-23	0.07294689607240201
1101	24-25	0.07816770515707816
1101	26-27	0.11349073835504697
1101	28-29	0.0790630820181164
1101	30-31	0.09682914223042616
1101	32-33	0.20416882169406847
1101	34-35	-0.12819157741832754
1101	36-37	-0.006712834607576212
1101	38-39	0.06286698012588943
1101	40-41	-0.20212590442969258
1101	42-43	-0.21979486777847512
1101	44-45	-0.04594871521489097
1101	46-47	0.06556069045497281
1101	48-49	-0.10751976306616484
1101	50-51	0.055856068399613434
1101	52-53	0.14326386287751092
1101	54-55	0.1621300340408638
1101	56-57	-0.017497953514272524
1101	58-59	0.09657763610078263
1101	60-61	0.028569863843522114
1101	62-63	0.15472198415424288
1101	64-65	0.1703449472263472
1101	66-67	-0.04000456643080241
1101	68-69	-0.08664421150036361
1101	70-71	-0.08230991450760428
1101	72-73	-0.06912734858402558
1101	74-75	0.05820880378166038
1101	76-77	-0.0717166758874157
1101	78-79	-0.09276690629366868
1101	80-81	-0.017564904962384276
1101	82-83	0.07560747262364487
1101	84-85	0.06567012229918845
1101	86-87	0.12334263250841815
1101	88-89	0.07150626072680666
1101	90-91	-0.0761049836206702
1101	92-93	-0.18337747053400122
1101	94-95	-0.10347858924136588
1101	96-97	0.037013056894224405
1101	98-99	-0.35673944134434166
1101	100-101	-0.47339736555116474
1102	1	0.17256222848111236
1102	2	0.13371892671221275
1102	3	0.11999777018618119
1102	4	0.09361479108235216
1102	5	0.09906218713095427
1102	6	0.1288769213533456
1102	7	0.15225683229314058
1102	8	0.11375448239598285
1102	9	0.07999706932618267
1102	10-11	0.20689652397052072
1102	12-13	0.1366772808329486
1102	14-15	0.2342969212043755
1102	16-17	0.14005055960108592
1102	18-19	0.1329022429694291
1102	20-21	0.20178372928395305
1102	22-23	0.16061343907255576
1102	24-25	0.24478695082851942
1102	26-27	0.26930776026075876
1102	28-29	0.2765798955503911
1102	30-31	0.07899559364297204
1102	32-33	0.26896728711346896
1102	34-35	0.25363669721060234
1102	36-37	0.2253083318931246
1102	38-39	0.06352668985361731
1102	40-41	0.2078272045744285
1102	42-43	0.33498660976675865
1102	44-45	0.3400781771385226
1102	46-47	0.27920715112855277
1102	48-49	0.3211492969660199
1102	50-51	0.28198959290464387
1102	52-53	0.29138892041033415
1102	54-55	0.3684518105987138
1102	56-57	0.4746929243599354
1102	58-59	0.43147260789458386
1102	60-61	0.5060123472903371
1102	62-63	0.45096977466130284
1102	64-65	0.4426953456092022
1102	66-67	0.38622045270032146
1102	68-69	0.4253793353483246
1102	70-71	0.36135626772105667
1102	72-73	0.41563795933165437
1102	74-75	0.34505236005876583
1102	76-77	0.3898523267746654
1102	78-79	0.43089245103097795
1102	80-81	0.3425120100322232
1102	82-83	0.423051806419096
1102	84-85	0.49888229963883646
1102	86-87	0.5407594342319371
1102	88-89	0.4271762775473107
1102	90-91	0.29863385222179417
1102	92-93	0.4087926722775812
1102	94-95	0.46428508311489125
1102	96-97	0.3411857325448473
1102	98-99	0.22605064264660868
1102	100-101	0.23217822769943552
1103	1	0.19876475000454974
1103	2	0.06200959274320894
1103	3	0.15526655571801484
1103	4	0.06811006379527385
1103	5	0.07797627505884464
1103	6	0.13480810909909025
1103	7	0.16968852943500679
1103	8	0.1353952136387946
1103	9	0.1653953168820692
1103	10-11	0.15278096772665606
1103	12-13	0.07120691058066342
1103	14-15	0.15622291933482302
1103	16-17	0.17029604905408746
1103	18-19	0.20312938100337874
1103	20-21	0.22084451975133845
1103	22-23	0.11682080909556447
1103	24-25	-0.018968072910404032
1103	26-27	0.10423362073025544
1103	28-29	0.15562223084690885
1103	30-31	0.09909946945659698
1103	32-33	0.07726062538640122
1103	34-35	0.048363599652013534
1103	36-37	0.14889657332005157
1103	38-39	-0.00788347195367578
1103	40-41	-0.03622496943103215
1103	42-43	0.03672056834396642
1103	44-45	0.065093474205284
1103	46-47	0.06145517718696425
1103	48-49	0.10607089348886944
1103	50-51	-0.1661406981217297
1103	52-53	-0.19984525300905176
1103	54-55	-0.023939840872110096
1103	56-57	-0.04621610351590988
1103	58-59	-0.06351693847715723
1103	60-61	-0.13271098153358452
1103	62-63	-0.28658652335237633
1103	64-65	-0.09598598903394162
1103	66-67	-0.13211723820935362
1103	68-69	-0.13370662885274243
1103	70-71	-0.11730577920614849
1103	72-73	-0.16194442340727733
1103	74-75	-0.12229067988973696
1103	76-77	-0.09261513437786562
1103	78-79	-0.06428005281416915
1103	80-81	-0.08277358582458305
1103	82-83	0.029691024403689426
1103	84-85	-0.09408451648106109
1103	86-87	-0.10313579617048418
1103	88-89	-0.03826146149307874
1103	90-91	0.012710544553119263
1103	92-93	-0.026322459342988935
1103	94-95	-0.07483403358768825
1103	96-97	-0.1719358990005624
1103	98-99	-0.010308650403690223
1103	100-101	0.06844394167136159
1104	1	0.1714335092952055
1104	2	0.12571188769972963
1104	3	0.1575216899182621
1104	4	0.09319227053754275
1104	5	0.12864773992492218
1104	6	0.12390406430288436
1104	7	0.17528732344330678
1104	8	0.15264447697463623
1104	9	0.12497053568523597
1104	10-11	0.17062268570848715
1104	12-13	0.20405107841613557
1104	14-15	0.2188115557228585
1104	16-17	0.1757675921983477
1104	18-19	0.2596559716757838
1104	20-21	0.32369701318504696
1104	22-23	0.21020366656190959
1104	24-25	0.22605377080454758
1104	26-27	0.290345512671216
1104	28-29	0.2406253200049946
1104	30-31	0.2617776816685833
1104	32-33	0.3504063741094967
1104	34-35	0.3069300350954407
1104	36-37	0.3449557494860116
1104	38-39	0.3270519046161624
1104	40-41	0.3680407772228733
1104	42-43	0.426844998460183
1104	44-45	0.3744463029078062
1104	46-47	0.3240129951970019
1104	48-49	0.38302221167605666
1104	50-51	0.4645406930437659
1104	52-53	0.37404674943885396
1104	54-55	0.3213260365707882
1104	56-57	0.27375381501425977
1104	58-59	0.2059359730909307
1104	60-61	0.4193951361706638
1104	62-63	0.42369522445714836
1104	64-65	0.45574984451656775
1104	66-67	0.3867745739112749
1104	68-69	0.41029008287757307
1104	70-71	0.5087035653750362
1104	72-73	0.37208987551976946
1104	74-75	0.4286976310874664
1104	76-77	0.4611828219952159
1104	78-79	0.511598307560277
1104	80-81	0.46377790355954573
1104	82-83	0.3908735082429544
1104	84-85	0.49814320083120833
1104	86-87	0.49010541388152973
1104	88-89	0.4070267672041794
1104	90-91	0.4359578987599839
1104	92-93	0.5057464664596374
1104	94-95	0.5396588027864873
1104	96-97	0.5356780948367366
1104	98-99	0.5903024953567488
1104	100-101	0.6941943923565894
1105	1	0.08941196343337054
1105	2	0.028590508899476674
1105	3	-0.022918637853145185
1105	4	0.010328507583707847
1105	5	0.019635548621721455
1105	6	-0.026413886701909917
1105	7	0.031671860656025785
1105	8	0.0351569492120376
1105	9	-0.018548784133123775
1105	10-11	0.002932484820036052
1105	12-13	0.10747411942410423
1105	14-15	0.043781524219355106
1105	16-17	0.04428142566092674
1105	18-19	0.08438634393058919
1105	20-21	0.09211284171377088
1105	22-23	0.13205606169288586
1105	24-25	0.13282688487414163
1105	26-27	0.01851912649687648
1105	28-29	0.1622432715016302
1105	30-31	0.10413787562209365
1105	32-33	-0.03180806663469582
1105	34-35	-0.03794714899527918
1105	36-37	0.0751098967611128
1105	38-39	-0.026585473147008543
1105	40-41	-0.04376824934889356
1105	42-43	-0.10091736703626708
1105	44-45	0.046309413119971055
1105	46-47	0.0534290314694843
1105	48-49	0.0878609549738627
1105	50-51	0.04468961744868949
1105	52-53	0.04219564948599697
1105	54-55	-0.02882735889205179
1105	56-57	-0.11375750608382162
1105	58-59	-0.06431154879769707
1105	60-61	0.012116539609749566
1105	62-63	-0.08731917637776121
1105	64-65	-0.06905100135419673
1105	66-67	0.04193820081285082
1105	68-69	0.014387623608342892
1105	70-71	-0.05501193762600565
1105	72-73	-0.11097514359288851
1105	74-75	-0.05163106076155799
1105	76-77	-0.10389513578291343
1105	78-79	0.04469192364371821
1105	80-81	-0.06773099300257002
1105	82-83	-0.12160991265737664
1105	84-85	0.09266374252013776
1105	86-87	0.013942546521121812
1105	88-89	0.04375143739631682
1105	90-91	-0.012716511026408028
1105	92-93	0.02889180301037797
1105	94-95	0.06418966374267754
1105	96-97	0.04605866641546896
1105	98-99	0.13905008387795448
1105	100-101	0.09379718488240485
1106	1	0.029073524354700453
1106	2	0.01650862712801171
1106	3	0.005792753419264329
1106	4	0.06166134894890263
1106	5	0.06221352249460921
1106	6	0.08433767880464416
1106	7	0.014671595242397473
1106	8	0.013958788018982204
1106	9	0.003391000123201593
1106	10-11	0.0056481912329715556
1106	12-13	-0.01620757244202764
1106	14-15	0.06373849295104606
1106	16-17	0.08487908686187495
1106	18-19	0.11481302358390622
1106	20-21	0.05888157656733739
1106	22-23	0.047159298248509174
1106	24-25	0.09763204835936534
1106	26-27	0.0023458036139700766
1106	28-29	0.009245999722303111
1106	30-31	0.045109040311800186
1106	32-33	0.15983486371858646
1106	34-35	0.06910926041002341
1106	36-37	0.09656062360065931
1106	38-39	0.03658547213706953
1106	40-41	0.08571616744526978
1106	42-43	0.04996810887617187
1106	44-45	0.1578008775023747
1106	46-47	0.01941765494721892
1106	48-49	0.02478166971740592
1106	50-51	-0.0133329042210093
1106	52-53	0.08502084958628586
1106	54-55	0.0035094979387579883
1106	56-57	0.049619930109564336
1106	58-59	0.09001518271472975
1106	60-61	0.12613844368664218
1106	62-63	0.17144247662629652
1106	64-65	0.22784834236446017
1106	66-67	0.17662732759757915
1106	68-69	0.15913866318211234
1106	70-71	0.11713543879481136
1106	72-73	0.1124241486226083
1106	74-75	0.1561343567554303
1106	76-77	0.049187288607022595
1106	78-79	-0.0171781500469379
1106	80-81	-0.029771950351502596
1106	82-83	-0.04804806850211918
1106	84-85	-0.010530987651215185
1106	86-87	-0.05942919206714947
1106	88-89	0.016980257533759868
1106	90-91	1.8397043345785846E-4
1106	92-93	-0.017550797900746318
1106	94-95	0.0597545531466217
1106	96-97	0.10252608227679261
1106	98-99	0.0726862772192085
1106	100-101	0.03385725557186703
1107	1	0.23736991999339807
1107	2	0.15629459686562086
1107	3	0.15832956798824682
1107	4	0.11797002305614512
1107	5	0.10011301786887827
1107	6	0.0640799578793434
1107	7	0.12917371300355995
1107	8	0.14535114021137474
1107	9	0.1661728157908442
1107	10-11	0.1716841941197842
1107	12-13	0.16274111447028616
1107	14-15	0.2787669018266996
1107	16-17	0.20846397063127142
1107	18-19	0.2177753083034446
1107	20-21	0.19079259555521588
1107	22-23	0.2161216663146348
1107	24-25	0.2462204364741396
1107	26-27	0.23862498912833274
1107	28-29	0.29546518516090003
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2316	92-93	-0.2776247881485361
2316	94-95	-0.2283493010080555
2316	96-97	-0.2337882572506338
2316	98-99	-0.31364291015422907
2316	100-101	-0.10087305562259274
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	5.0
4	12.0
5	43.0
6	80.0
7	187.0
8	512.0
9	1046.0
10	1899.0
11	3269.0
12	5273.0
13	7414.0
14	9277.0
15	12245.0
16	16819.0
17	26075.0
18	27113.0
19	16121.0
20	10867.0
21	10293.0
22	10514.0
23	10888.0
24	11872.0
25	13988.0
26	16288.0
27	18556.0
28	23354.0
29	29650.0
30	37758.0
31	48645.0
32	62595.0
33	82913.0
34	117625.0
35	185568.0
36	326440.0
37	696672.0
38	1077518.0
39	324995.0
40	426.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.329423383624075	19.802219467221832	14.482791145557522	23.38556600359657
2	16.448169496153405	21.70849696608468	38.692998711483575	23.15033482627834
3	18.4989051595416	26.059452687636853	31.28571714746677	24.155925005354778
4	13.659041800623264	19.263580347758005	36.89468314319973	30.182694708419
5	13.799607188941627	36.67485716451806	34.777646936637716	14.747888709902593
6	29.294595041877553	36.733257992669536	18.690607205275366	15.28153976017754
7	25.15768994060374	33.41849478722529	23.237828204179156	18.185987067991817
8	24.40054400602561	33.961514624707654	21.728498094037352	19.90944327522939
9	25.569300210150526	16.363295680465185	21.66005047434108	36.40735363504321
10-11	23.708501897025318	26.526087603707698	28.912354687490847	20.853055811776134
12-13	25.015293620564734	25.12751874759039	28.41514637035001	21.44204126149487
14-15	22.500580926980714	26.681128718009724	26.92029496914153	23.897995385868036
16-17	21.153121023897945	28.98381444762066	28.01205260208215	21.851011926399245
18-19	20.926685571719652	27.271522552234543	30.63928366871234	21.16250820733346
20-21	21.97355043443128	26.368898461762253	30.6069186644424	21.05063243936407
22-23	21.82668544944137	26.58601394161828	30.13370245533107	21.45359815360928
24-25	21.437649642491394	26.884459123580772	30.520688840079423	21.157202393848404
26-27	21.06360864798397	27.033787110309486	30.81884028380772	21.083763957898825
28-29	20.973821440899453	26.76753045021944	30.930796636915037	21.32785147196607
30-31	21.351900793157334	26.616327918697408	30.726529527940947	21.305241760204314
32-33	21.2027804358646	26.77388387319462	30.530199718628026	21.493135972312754
34-35	21.366753717967963	27.097776601066375	30.469491411017042	21.065978269948623
36-37	21.338713224172956	27.046017390645073	30.305002765299644	21.310266619882327
38-39	21.152425545107782	27.04431405530728	30.633576562252973	21.169683837331966
40-41	21.310616284369814	26.945849334492518	30.489546868128464	21.25398751300921
42-43	21.450238950301355	26.96078083910433	30.13123698501333	21.45774322558098
44-45	21.444075274507007	27.143564644785823	29.986868288720135	21.425491791987035
46-47	21.58403694260724	27.18171779795286	29.65503139314174	21.57921386629816
48-49	21.485387311518647	27.394688205837188	29.53104905453836	21.58887542810581
50-51	21.555360440974024	27.607951388321744	29.205838110438894	21.63085006026534
52-53	21.66524337119782	27.78213686627012	29.009478808820344	21.54314095371172
54-55	21.668094071252707	27.771073068219255	28.8792403392857	21.681592521242337
56-57	21.638804536404447	28.025498514322766	28.73119327394939	21.604503675323397
58-59	21.456037292818955	28.052176909135117	29.035702277516044	21.45608352052988
60-61	21.63255488060169	28.01660899142106	28.9612122480371	21.389623879940157
62-63	21.545064443822877	28.074668072125302	28.857887635739644	21.522379848312177
64-65	21.583651732530807	28.05597353877305	28.767569741559246	21.592804987136905
66-67	21.50744806326932	28.12878601792664	28.62345897224031	21.740306946563734
68-69	21.426066262050718	28.053689020603606	28.77199378806758	21.748250929278093
70-71	21.49361696947545	27.89171769295286	28.814035440238573	21.800629897333117
72-73	21.581420392317735	27.92589016268468	28.57999448004131	21.912694964956277
74-75	21.596502269296415	28.07481497051425	28.405793840754136	21.9228889194352
76-77	21.56706172920929	28.090979287993672	28.41083340357478	21.931125579222257
78-79	21.513764775930998	28.07340597459816	28.37943370385392	22.033395545616933
80-81	21.46677589524905	28.195583269534474	28.310159244424227	22.027481590792252
82-83	21.590315778053526	28.15995344728639	28.261140257926197	21.98859051673389
84-85	21.608528117275018	28.082726891972232	28.163133727755213	22.145611262997537
86-87	21.547494102406176	28.11943765870803	28.212896164287265	22.120172074598532
88-89	21.58594293056139	28.034426759233195	28.23058667841469	22.149043631790725
90-91	21.54299018141704	27.92635577389353	28.30383474689555	22.22681929779388
92-93	21.573635739704272	27.94065119851135	28.353232246995745	22.13248081478863
94-95	21.53697727791737	27.91504112558582	28.255969442960883	22.292012153535932
96-97	21.599988782108326	27.887293472527645	28.186123930274636	22.32659381508939
98-99	21.457469166592315	27.806610943947064	28.239331869768293	22.496588019692332
100-101	21.450504448450538	27.921587324138773	28.10656125843913	22.52134696897156
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3131.0
1	1902.5
2	727.0
3	726.0
4	560.5
5	524.5
6	911.5
7	1917.5
8	3260.0
9	3996.0
10	3518.5
11	2902.0
12	2971.5
13	3292.0
14	3699.5
15	4017.0
16	4275.5
17	4612.0
18	4951.5
19	5353.5
20	5932.5
21	6876.5
22	8061.0
23	9663.0
24	11770.5
25	14678.0
26	19279.5
27	25093.0
28	30791.0
29	37636.0
30	46237.5
31	54361.0
32	62434.0
33	70391.0
34	77184.0
35	83333.0
36	91700.0
37	100378.0
38	105508.0
39	109146.0
40	111563.0
41	113862.0
42	116004.5
43	119050.0
44	123382.5
45	126853.0
46	128177.5
47	126734.5
48	124817.0
49	122063.5
50	116782.5
51	110542.5
52	104161.5
53	98249.5
54	90847.0
55	80881.5
56	70966.5
57	61882.5
58	55013.0
59	48109.0
60	39804.5
61	32396.0
62	25725.0
63	19260.0
64	13908.5
65	9897.5
66	7147.5
67	5528.0
68	4337.5
69	3205.5
70	2303.0
71	1651.5
72	1138.0
73	748.5
74	540.5
75	431.0
76	287.5
77	151.0
78	87.0
79	63.5
80	40.0
81	25.0
82	18.0
83	10.5
84	8.5
85	7.0
86	4.5
87	3.5
88	2.5
89	2.5
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.018953303067630625
2	0.0
3	0.0016025557065313697
4	0.018706756035856566
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	9.245513691527134E-5
16-17	0.001248144348356163
18-19	1.6950108434466414E-4
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.4686649509047814E-4
28-29	0.005454853078001009
30-31	1.5409189485878557E-5
32-33	6.163675794351423E-5
34-35	0.011402800219550133
36-37	0.004360800624503632
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	1.5409189485878557E-5
58-59	3.0818378971757114E-4
60-61	0.0021110589595653624
62-63	0.010000563976335183
64-65	0.0021418773385371193
66-67	0.0045611200878200526
68-69	0.0028969276233451685
70-71	7.704594742939278E-5
72-73	0.007011181216074743
74-75	0.006132857415379666
76-77	0.0017412384119042767
78-79	4.0063892663284243E-4
80-81	0.007550502848080493
82-83	0.009954336407877547
84-85	0.0020956497700794835
86-87	0.0018953303067630626
88-89	4.776848740622352E-4
90-91	1.5409189485878557E-5
92-93	0.0
94-95	0.0
96-97	1.5409189485878557E-5
98-99	1.5409189485878557E-5
100-101	0.0010940524534973775
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3244817.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.17341853704729
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.66958019388423	25.032890842232142
2	21.038379240286165	18.58674263044576
3	9.461595787281915	12.538530922199026
4	4.773963395012228	8.435291325136737
5	2.6338172024873527	5.817235481777443
6	1.5057311567439702	3.9907975554674255
7	0.998885276748058	3.08869241802004
8	0.6568381070152417	2.3211827689812905
9	0.4616014092963706	1.8351461025125506
>10	1.7041443242578365	12.180405520574435
>50	0.06799447554888968	2.0657648717163872
>100	0.025444632396123466	1.8672835351432635
>500	0.0011869511634222897	0.37340616283985495
>1k	5.585652521560738E-4	0.5380725122945451
>5k	1.3964131303901845E-4	0.37704553839280147
>10k+	1.3964131303901845E-4	0.9515118122663655
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15545	0.47907170111596437	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15306	0.47170610854171435	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7117	0.21933440314199537	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	5108	0.15742027978773535	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4528	0.13954561998411621	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.0818378971757115E-5
7	0.0	0.0	0.0	0.0	3.0818378971757115E-5
8	0.0	0.0	0.0	0.0	3.0818378971757115E-5
9	0.0	0.0	0.0	0.0	3.0818378971757115E-5
10-11	0.0	0.0	0.0	3.0818378971757115E-5	3.0818378971757115E-5
12-13	0.0	0.0	0.0	3.0818378971757115E-5	3.0818378971757115E-5
14-15	0.0	0.0	0.0	3.0818378971757115E-5	3.0818378971757115E-5
16-17	0.0	0.0	0.0	3.0818378971757115E-5	3.0818378971757115E-5
18-19	0.0	0.0	0.0	3.0818378971757115E-5	3.0818378971757115E-5
20-21	0.0	0.0	0.0	6.163675794351423E-5	3.0818378971757115E-5
22-23	0.0	0.0	0.0	3.0818378971757114E-4	3.0818378971757115E-5
24-25	0.0	0.0	0.0	5.54730821491628E-4	3.0818378971757115E-5
26-27	0.0	0.0	0.0	0.0011248708324691345	3.0818378971757115E-5
28-29	0.0	0.0	0.0	0.0023113784228817836	3.0818378971757115E-5
30-31	0.0	0.0	0.0	0.005840082815147973	3.0818378971757115E-5
32-33	0.0	0.0	0.0	0.013822042968833066	3.0818378971757115E-5
34-35	0.0	0.0	0.0	0.02676576213697105	3.0818378971757115E-5
36-37	0.0	0.0	0.0	0.04422437382447146	3.0818378971757115E-5
38-39	0.0	0.0	0.0	0.06286949310238452	3.0818378971757115E-5
40-41	0.0	0.0	0.0	0.07921864314690166	3.0818378971757115E-5
42-43	0.0	0.0	0.0	0.1003908695004988	3.0818378971757115E-5
44-45	0.0	0.0	0.0	0.1288362332914306	3.0818378971757115E-5
46-47	0.0	0.0	0.0	0.15933101928398427	3.0818378971757115E-5
48-49	0.0	0.0	0.0	0.1857731884417519	3.0818378971757115E-5
50-51	0.0	0.0	0.0	0.21048952837710108	3.0818378971757115E-5
52-53	0.0	0.0	0.0	0.23953585055798216	3.0818378971757115E-5
54-55	0.0	3.0818378971757115E-5	0.0	0.27679527073483656	3.0818378971757115E-5
56-57	0.0	3.0818378971757115E-5	0.0	0.3186928569469403	3.0818378971757115E-5
58-59	0.0	3.0818378971757115E-5	0.0	0.3604517604536712	3.0818378971757115E-5
60-61	0.0	3.0818378971757115E-5	0.0	0.40160970557045284	3.0818378971757115E-5
62-63	0.0	3.0818378971757115E-5	0.0	0.44863855188135415	3.0818378971757115E-5
64-65	0.0	3.0818378971757115E-5	0.0	0.5038342686197712	3.0818378971757115E-5
66-67	0.0	3.0818378971757115E-5	0.0	0.5672893109226191	3.0818378971757115E-5
68-69	0.0	3.0818378971757115E-5	0.0	0.6350126987130553	3.0818378971757115E-5
70-71	0.0	3.0818378971757115E-5	0.0	0.703198362188068	3.0818378971757115E-5
72-73	0.0	3.0818378971757115E-5	0.0	0.7705211110518713	3.0818378971757115E-5
74-75	0.0	3.0818378971757115E-5	0.0	0.852282270463943	3.0818378971757115E-5
76-77	0.0	3.0818378971757115E-5	0.0	0.944013175473378	3.0818378971757115E-5
78-79	0.0	3.0818378971757115E-5	0.0	1.0415225265400174	3.0818378971757115E-5
80-81	0.0	3.0818378971757115E-5	0.0	1.144132319326483	3.0818378971757115E-5
82-83	0.0	3.0818378971757115E-5	0.0	1.254123113876684	6.163675794351423E-5
84-85	0.0	3.0818378971757115E-5	0.0	1.3942851014402353	6.163675794351423E-5
86-87	0.0	3.0818378971757115E-5	0.0	1.5662054285341824	6.163675794351423E-5
88-89	0.0	3.0818378971757115E-5	0.0	1.755045045683624	6.163675794351423E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7080	0.0	64.963066	1
GTATCAA	12860	0.0	41.52884	1
GTGGTAT	2745	0.0	33.926434	1
TCAACGC	16160	0.0	32.983925	4
ATCAACG	16245	0.0	32.89958	3
TATCAAC	16560	0.0	32.331146	2
CAACGCA	16620	0.0	32.035515	5
TGGTATC	2840	0.0	31.782845	2
AACGCAG	17080	0.0	31.256157	6
ACGCAGA	19310	0.0	27.52358	7
CGCAGAG	19620	0.0	27.064493	8
GCAGAGT	21275	0.0	24.445656	9
GAGTACT	13990	0.0	23.849854	12-13
CAGAGTA	20275	0.0	23.261917	10-11
GTACATG	10675	0.0	22.744556	1
TACATGG	10710	0.0	21.646507	2
GTACTTT	15615	0.0	21.200588	14-15
ACATGGG	10655	0.0	20.598995	3
AGAGTAC	19530	0.0	20.136555	10-11
CATGGGG	6080	0.0	19.377552	4
>>END_MODULE