FastQCFastQC Report
Fri 10 Feb 2017
SRR2031721_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031721_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3066226
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70450.2297612765660457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61990.20217035534888816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31080.1013623914218978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47150.053.1996961
GTATCAA82350.038.536281
ATCAACG91600.033.8668373
TCAACGC91550.033.8334434
TATCAAC93700.033.5641252
CAACGCA93350.033.129085
AACGCAG96350.032.3859836
ACGCAGA110050.028.0953467
CGCAGAG113500.026.9105388
GCAGAGT126500.023.0936139
GAGTACT78350.022.10208112-13
GTGGTAT20600.021.6779171
CAGAGTA119800.021.11621910-11
TGGTATC23100.019.537482
GTACTTT88850.019.38334714-15
GTACATG67850.019.1147981
AGAGTAC109500.018.67722710-11
TACATGG68450.018.3920172
CTATACG1901.8648764E-417.5025524
ACATGGG69550.017.3497623
Produced by FastQC (version 0.11.5)