##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031721_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3066226 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02712422371997 33.0 31.0 34.0 30.0 34.0 2 32.33942899186166 34.0 31.0 34.0 31.0 34.0 3 32.448558586353386 34.0 31.0 34.0 31.0 34.0 4 35.87997655750098 37.0 37.0 37.0 35.0 37.0 5 35.88267988073938 37.0 37.0 37.0 35.0 37.0 6 35.908092554169194 37.0 37.0 37.0 35.0 37.0 7 35.89008703207135 37.0 37.0 37.0 35.0 37.0 8 35.88130490055202 37.0 37.0 37.0 35.0 37.0 9 37.665954499113894 39.0 38.0 39.0 35.0 39.0 10-11 37.592629179975646 39.0 38.0 39.0 35.0 39.0 12-13 37.54415036595476 39.0 38.0 39.0 35.0 39.0 14-15 38.94958085281385 41.0 39.0 41.0 36.0 41.0 16-17 38.881083781821694 41.0 39.0 41.0 36.0 41.0 18-19 38.88864992339117 41.0 39.0 41.0 36.0 41.0 20-21 38.89166128002306 41.0 39.0 41.0 35.5 41.0 22-23 38.79007043185988 41.0 39.0 41.0 35.0 41.0 24-25 38.724958956058686 41.0 39.0 41.0 35.0 41.0 26-27 38.6133460155905 40.5 39.0 41.0 35.0 41.0 28-29 38.50135590135886 40.0 38.0 41.0 34.0 41.0 30-31 38.34347451883847 40.0 38.0 41.0 34.0 41.0 32-33 38.14936146259278 40.0 38.0 41.0 34.0 41.0 34-35 37.98720381341754 40.0 38.0 41.0 34.0 41.0 36-37 37.82448537713789 40.0 38.0 41.0 33.0 41.0 38-39 37.63633665620212 40.0 38.0 41.0 33.0 41.0 40-41 37.54187297348597 40.0 38.0 41.0 33.0 41.0 42-43 37.51101060391504 40.0 38.0 41.0 33.0 41.0 44-45 37.37395107209971 40.0 38.0 41.0 32.5 41.0 46-47 37.29622115264824 40.0 37.5 41.0 32.0 41.0 48-49 37.33241026590995 40.0 38.0 41.0 32.0 41.0 50-51 36.776274971251304 39.5 37.0 40.5 31.5 41.0 52-53 36.798130340033644 39.5 37.0 40.5 31.0 41.0 54-55 37.02624708680965 40.0 37.0 41.0 32.0 41.0 56-57 36.89607370754798 40.0 37.0 41.0 31.0 41.0 58-59 36.70681564242166 40.0 36.0 41.0 31.0 41.0 60-61 36.46930346947681 39.5 36.0 41.0 31.0 41.0 62-63 36.18968481123048 39.0 35.0 41.0 30.0 41.0 64-65 35.91806474799966 39.0 35.0 41.0 30.0 41.0 66-67 35.628857266228906 38.5 35.0 41.0 29.5 41.0 68-69 35.29566215927984 37.5 35.0 40.0 29.0 41.0 70-71 34.93845153618814 37.0 35.0 40.0 29.0 41.0 72-73 34.56964979098083 36.5 35.0 39.0 29.0 41.0 74-75 34.16585633283391 36.0 34.5 39.0 28.0 41.0 76-77 33.7876549217181 35.5 34.0 38.5 28.0 40.0 78-79 33.39633983274553 35.0 34.0 37.0 27.0 39.0 80-81 33.040463586180536 35.0 34.0 37.0 26.0 39.0 82-83 32.70862780499546 35.0 34.0 36.5 26.0 39.0 84-85 32.379609983086695 35.0 34.0 36.0 26.0 37.0 86-87 32.095146606936346 35.0 34.0 36.0 25.0 37.0 88-89 31.820303200090272 35.0 33.0 35.0 25.0 36.5 90-91 31.578204607227256 35.0 33.0 35.0 24.0 36.0 92-93 31.357660198563316 35.0 33.0 35.0 23.5 36.0 94-95 31.10218261798054 35.0 33.0 35.0 21.0 36.0 96-97 30.838518426234728 35.0 33.0 35.0 18.5 35.5 98-99 30.58793008082248 35.0 32.0 35.0 9.5 35.0 100-101 30.007992235406 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.10623441395190625 1101 2 0.07143166785289878 1101 3 0.1772242645681672 1101 4 0.28231381500584973 1101 5 0.12608341767143827 1101 6 0.21814168734191952 1101 7 0.19467260559919453 1101 8 0.2305919574312867 1101 9 0.24077525777692443 1101 10-11 0.12959709715122614 1101 12-13 0.15576090369930284 1101 14-15 0.18522645328998522 1101 16-17 0.25212827825207285 1101 18-19 0.2283422268874702 1101 20-21 0.25399650488790115 1101 22-23 0.281280884708508 1101 24-25 0.06552650262604942 1101 26-27 0.05549281062528877 1101 28-29 0.01626220267029055 1101 30-31 0.08704754157851369 1101 32-33 -0.06842511216321867 1101 34-35 -0.03790266382629426 1101 36-37 -0.2142860490104539 1101 38-39 0.05046153073985238 1101 40-41 -0.05976398342452427 1101 42-43 -0.0939424344990627 1101 44-45 -0.32524859166173314 1101 46-47 -0.2944739574141124 1101 48-49 -0.042572109060955654 1101 50-51 0.03006023575257899 1101 52-53 0.07976328040233938 1101 54-55 0.15951732170214683 1101 56-57 0.02097187777885523 1101 58-59 -0.16746907328651872 1101 60-61 -0.21487304970765564 1101 62-63 -0.14162452602852937 1101 64-65 0.10936071529479108 1101 66-67 -0.19299533196024754 1101 68-69 -0.21195736526806996 1101 70-71 -0.3240968099587107 1101 72-73 0.03491215307516171 1101 74-75 0.045540063114501095 1101 76-77 -0.11840365264909991 1101 78-79 -0.2825783144373588 1101 80-81 -0.29018562528212755 1101 82-83 -0.21396303092777202 1101 84-85 -0.13372906039086985 1101 86-87 -0.23366755278667029 1101 88-89 -0.4428063409123517 1101 90-91 -0.7397907972616835 1101 92-93 -0.5043388743756374 1101 94-95 -0.5826803383815324 1101 96-97 -0.622752271015834 1101 98-99 -0.5609030606423495 1101 100-101 -0.6450366133495784 1102 1 -0.031014591363472732 1102 2 0.0839813029383194 1102 3 0.18889927604710977 1102 4 0.22584429387676153 1102 5 0.17285753645487745 1102 6 0.12226524401722116 1102 7 0.1700256027471525 1102 8 0.02268758378080804 1102 9 0.1705241116050047 1102 10-11 0.2378190445190853 1102 12-13 0.2524627192205884 1102 14-15 0.3710989251814425 1102 16-17 0.23843904854099662 1102 18-19 0.2636536868527273 1102 20-21 0.2352107330173041 1102 22-23 0.26991469288076075 1102 24-25 0.10639867384117707 1102 26-27 0.1417393896489827 1102 28-29 0.24418003158670842 1102 30-31 0.067754769900219 1102 32-33 0.17191307667530253 1102 34-35 0.27907972343104603 1102 36-37 0.20759228857514955 1102 38-39 0.11052836231913687 1102 40-41 0.3018670384803741 1102 42-43 0.27300844438411787 1102 44-45 0.07432989770278198 1102 46-47 0.1481574678982014 1102 48-49 0.26043297863318315 1102 50-51 0.22912613460881914 1102 52-53 0.1302941307338088 1102 54-55 0.1498864394864654 1102 56-57 0.18082605915308392 1102 58-59 0.095347255442924 1102 60-61 0.10598171986465132 1102 62-63 0.09560917966320659 1102 64-65 0.1867845580510945 1102 66-67 0.09743318506022547 1102 68-69 0.06902138004716818 1102 70-71 0.04765698185174472 1102 72-73 0.1951005236662624 1102 74-75 -0.18433866096548002 1102 76-77 -0.045632384653153224 1102 78-79 -0.12066896334073363 1102 80-81 -0.007904262391477346 1102 82-83 0.14161821087801485 1102 84-85 0.12685427675311445 1102 86-87 0.20021340819995004 1102 88-89 0.14106082341540827 1102 90-91 0.15052318086787153 1102 92-93 0.24865645699786754 1102 94-95 0.011365424556476711 1102 96-97 -0.2488931184595522 1102 98-99 -0.145874897292412 1102 100-101 -0.1878269273120452 1103 1 0.003781004582741332 1103 2 0.2066458332633303 1103 3 0.2019872168738246 1103 4 0.14297555985365307 1103 5 0.18873410438547467 1103 6 0.13545365732979064 1103 7 0.08657406937791734 1103 8 0.23420721097779307 1103 9 0.2511600582443947 1103 10-11 0.19125343569118058 1103 12-13 0.30744503488451613 1103 14-15 0.43760552336575387 1103 16-17 0.4280530220539731 1103 18-19 0.34369674270129025 1103 20-21 0.20604866765656737 1103 22-23 0.22005389588940716 1103 24-25 0.2594351379875306 1103 26-27 0.3653666879939266 1103 28-29 0.18614229164604268 1103 30-31 0.3868843334430281 1103 32-33 0.25151070941388554 1103 34-35 0.2403147276915476 1103 36-37 0.2451813731971484 1103 38-39 0.4019175756401978 1103 40-41 0.4520006285832707 1103 42-43 0.3975574231004515 1103 44-45 0.28907628275577935 1103 46-47 0.2779145896590691 1103 48-49 0.371442553278051 1103 50-51 0.4244629148457335 1103 52-53 0.36751291089765203 1103 54-55 0.2388905509030863 1103 56-57 0.2973616941804451 1103 58-59 0.2565811400686826 1103 60-61 0.32428125103233896 1103 62-63 0.3303053932942319 1103 64-65 0.476450123154315 1103 66-67 0.392993995369352 1103 68-69 0.37943458714070033 1103 70-71 0.41855698255888285 1103 72-73 0.36517200939798755 1103 74-75 0.3300638930981492 1103 76-77 0.2963889950668417 1103 78-79 0.3397216006133874 1103 80-81 0.3565080442800195 1103 82-83 0.265062360550786 1103 84-85 0.19089738386916366 1103 86-87 0.3627068819079753 1103 88-89 0.4118144318803161 1103 90-91 0.34642287220225043 1103 92-93 -0.17533514986655163 1103 94-95 0.2713450530970256 1103 96-97 0.29173931016962484 1103 98-99 0.2325069889032001 1103 100-101 0.2711570218400361 1104 1 -0.017593836947355612 1104 2 0.050949575928200375 1104 3 0.1649191375400747 1104 4 0.10811912081377528 1104 5 0.019958113657210674 1104 6 0.1376544739354415 1104 7 0.08129904778604669 1104 8 0.06401664745773417 1104 9 0.08714260490836523 1104 10-11 0.23556117479477479 1104 12-13 0.2022948464968337 1104 14-15 0.2864152506976154 1104 16-17 0.24837036149149583 1104 18-19 0.20613724016910595 1104 20-21 0.1978609638104345 1104 22-23 0.22656156905176061 1104 24-25 0.25427154487827863 1104 26-27 0.2780655940798411 1104 28-29 0.2285606033282903 1104 30-31 0.3003504725057766 1104 32-33 0.23053356904342337 1104 34-35 0.2596902397352565 1104 36-37 0.3380902477333976 1104 38-39 0.24413290153866996 1104 40-41 0.30787683667339394 1104 42-43 0.3054915473586135 1104 44-45 0.3174407291047032 1104 46-47 0.32129926242844675 1104 48-49 0.44981632465066923 1104 50-51 0.4407328609604235 1104 52-53 0.3980368218125889 1104 54-55 0.3539684204015998 1104 56-57 0.30561253620788165 1104 58-59 0.25170326457551795 1104 60-61 0.3505737936776683 1104 62-63 0.22733354154434693 1104 64-65 0.2116929956168505 1104 66-67 0.2921914605170741 1104 68-69 0.2963853987508003 1104 70-71 0.3287477070466167 1104 72-73 0.19889323781443835 1104 74-75 0.26917579005219494 1104 76-77 0.16842927139837371 1104 78-79 0.10166262649920554 1104 80-81 0.13449879585472502 1104 82-83 0.0301189434624618 1104 84-85 0.14153081940771273 1104 86-87 0.08061825421842883 1104 88-89 0.10047298281603645 1104 90-91 0.13037123155314134 1104 92-93 -0.04474665761187069 1104 94-95 0.17582822475960924 1104 96-97 0.05838725278076495 1104 98-99 -0.047371108614346014 1104 100-101 -0.13920119228470895 1105 1 -0.05561706704860825 1105 2 0.1818216846106182 1105 3 0.08337640981098815 1105 4 0.14225765463240236 1105 5 0.24903650765087804 1105 6 0.2797378889259079 1105 7 0.20578067781267606 1105 8 0.3339028235248378 1105 9 0.31777152379584095 1105 10-11 0.26466128871303596 1105 12-13 0.19645863202030966 1105 14-15 0.262171727636769 1105 16-17 0.31904644588951925 1105 18-19 0.2610632352246043 1105 20-21 0.2999443473607286 1105 22-23 0.09878487712465756 1105 24-25 0.18236117140037322 1105 26-27 0.20871235526966103 1105 28-29 0.10907023293152918 1105 30-31 0.33340366509888497 1105 32-33 0.3446459655921288 1105 34-35 0.23165843694483357 1105 36-37 0.2723124124317309 1105 38-39 0.3185980516707758 1105 40-41 0.3272263026619342 1105 42-43 0.2772036380991665 1105 44-45 0.3124150162772139 1105 46-47 0.29575319889622165 1105 48-49 0.2972317584255961 1105 50-51 0.20944893831584466 1105 52-53 0.245012224063494 1105 54-55 0.2523511375248617 1105 56-57 0.2510350031511521 1105 58-59 0.19253821620479528 1105 60-61 0.2458903072131946 1105 62-63 0.3112948051461544 1105 64-65 0.32738748824046127 1105 66-67 0.26706486139089947 1105 68-69 0.31820304989684445 1105 70-71 0.2240845256986006 1105 72-73 0.07472129356417412 1105 74-75 0.19878017826297167 1105 76-77 0.36938992610868837 1105 78-79 0.35364361986218285 1105 80-81 0.29740242226992564 1105 82-83 0.3343115963115153 1105 84-85 0.3651352160025354 1105 86-87 0.34499637269131966 1105 88-89 0.286470469697619 1105 90-91 0.11290961739332062 1105 92-93 0.25996371020680087 1105 94-95 0.4383346044962053 1105 96-97 0.3619621060861249 1105 98-99 0.32001892673489607 1105 100-101 0.3113275529481747 1106 1 0.0587151448767429 1106 2 0.15396960208862964 1106 3 0.2092367734996543 1106 4 0.15169082619465968 1106 5 0.21867822261000214 1106 6 0.23495213220847688 1106 7 0.2422959029138454 1106 8 0.2785387271833457 1106 9 0.29430374473567156 1106 10-11 0.30070382338205803 1106 12-13 0.3086783285564678 1106 14-15 0.3828773790569784 1106 16-17 0.30358933582019176 1106 18-19 0.3693754967903615 1106 20-21 0.29170356859073365 1106 22-23 0.30380876549502034 1106 24-25 0.3326783114200538 1106 26-27 0.2656836750451461 1106 28-29 0.2518700314731035 1106 30-31 0.26847238277905916 1106 32-33 0.34167941690710535 1106 34-35 0.41239406404114476 1106 36-37 0.3424007492653516 1106 38-39 0.4022009542318514 1106 40-41 0.30002226245330377 1106 42-43 0.21671755762012168 1106 44-45 0.22551619469249573 1106 46-47 0.20756025510656428 1106 48-49 0.19294341105820223 1106 50-51 0.24981962586233664 1106 52-53 0.21833265690030856 1106 54-55 0.22635208759022163 1106 56-57 0.09960896822055787 1106 58-59 0.22083171701837756 1106 60-61 0.03766191069156122 1106 62-63 0.1143471686362929 1106 64-65 0.19118712421755646 1106 66-67 0.15193356397942637 1106 68-69 0.2772440156413225 1106 70-71 0.14868353329558204 1106 72-73 0.08879312377189308 1106 74-75 0.18754866870736464 1106 76-77 0.2354708457476491 1106 78-79 0.16860357886150013 1106 80-81 0.2150964272866389 1106 82-83 0.3070230453586902 1106 84-85 0.30373597635868066 1106 86-87 0.23667676786988778 1106 88-89 0.20787357847757448 1106 90-91 0.2910497834319301 1106 92-93 0.28621407673654176 1106 94-95 0.4501306584838183 1106 96-97 0.4123394987847 1106 98-99 0.4172383143478875 1106 100-101 0.4691612747988181 1107 1 0.10489951827423027 1107 2 0.31391058994274346 1107 3 0.30332902317432087 1107 4 0.32031944869950735 1107 5 0.32987610937082223 1107 6 0.3889363384816207 1107 7 0.33689248919536396 1107 8 0.31775738118144403 1107 9 0.3538626962870737 1107 10-11 0.360379928675691 1107 12-13 0.3919424898044781 1107 14-15 0.4132620719911415 1107 16-17 0.44564813786992374 1107 18-19 0.44924550707290223 1107 20-21 0.34964062547704344 1107 22-23 0.5281125375412188 1107 24-25 0.47499139861101725 1107 26-27 0.4245796726869955 1107 28-29 0.5154402186703635 1107 30-31 0.5311925934467681 1107 32-33 0.4802324047971993 1107 34-35 0.5208648021983677 1107 36-37 0.46640510783438316 1107 38-39 0.5206500027397212 1107 40-41 0.4660384425030628 1107 42-43 0.5106314901702618 1107 44-45 0.48776170448260814 1107 46-47 0.420478945778882 1107 48-49 0.3221057095418658 1107 50-51 0.3563803148083622 1107 52-53 0.36965811489140066 1107 54-55 0.4510945742713375 1107 56-57 0.3614697427397502 1107 58-59 0.450816089463288 1107 60-61 0.4481377308471366 1107 62-63 0.5385713211205925 1107 64-65 0.49715823414441473 1107 66-67 0.45657457210226227 1107 68-69 0.5151415247053563 1107 70-71 0.5415368110337013 1107 72-73 0.48283923961266595 1107 74-75 0.4683392652904743 1107 76-77 0.3990178668011808 1107 78-79 0.5129698928806334 1107 80-81 0.5193393375999165 1107 82-83 0.5460766786197198 1107 84-85 0.41886489707304264 1107 86-87 0.469178721975787 1107 88-89 0.3705309322927235 1107 90-91 0.47368314024451763 1107 92-93 0.5975526273694491 1107 94-95 0.6544958029500627 1107 96-97 0.7932772949766154 1107 98-99 0.8322777722045558 1107 100-101 0.7979413120657455 1108 1 -0.015279028815861295 1108 2 0.07864186067101286 1108 3 0.13930876057835206 1108 4 0.15492487325995086 1108 5 0.16352746551093134 1108 6 0.1946130455826136 1108 7 0.23259236652942405 1108 8 0.1508408394523073 1108 9 0.15977792049314843 1108 10-11 0.11270263096091071 1108 12-13 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-0.5241354934000597 2316 58-59 -0.429845085902798 2316 60-61 -0.5149056861555437 2316 62-63 -0.5221771541631313 2316 64-65 -0.5922906644508856 2316 66-67 -0.5086225514933105 2316 68-69 -0.5831912549706502 2316 70-71 -0.5597298420044865 2316 72-73 -0.41842258277989686 2316 74-75 -0.46240852915225616 2316 76-77 -0.3958948857882021 2316 78-79 -0.44250945068363734 2316 80-81 -0.4674351073046381 2316 82-83 -0.4055444492940339 2316 84-85 -0.3005915520313778 2316 86-87 -0.2923414927009027 2316 88-89 -0.3371794344477905 2316 90-91 -0.339884218032914 2316 92-93 -0.4122333680288328 2316 94-95 -0.4430686677914544 2316 96-97 -0.3605058706434683 2316 98-99 -0.29311996845348176 2316 100-101 -0.3762684398175935 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14202.0 3 2036.0 4 1736.0 5 1852.0 6 2162.0 7 2557.0 8 2947.0 9 3697.0 10 4234.0 11 5057.0 12 6217.0 13 7957.0 14 9037.0 15 10561.0 16 12360.0 17 16561.0 18 15762.0 19 10184.0 20 9412.0 21 9534.0 22 10247.0 23 11345.0 24 12882.0 25 14913.0 26 17588.0 27 21083.0 28 25064.0 29 30993.0 30 37866.0 31 48006.0 32 62174.0 33 82655.0 34 117822.0 35 184147.0 36 322039.0 37 639322.0 38 931087.0 39 348526.0 40 402.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.6256620215603 20.358206127751586 14.378969475559709 23.637162375128405 2 15.762393753131631 22.027063839906035 39.304726895491164 22.905815511471168 3 18.088946213745142 26.25978502743417 31.234744959733064 24.416523799087628 4 13.341795231004253 18.97172265052504 38.01974609734165 29.666736021129047 5 13.86442701740222 37.012052615558666 35.086325258629586 14.037195108409527 6 29.376192634368508 37.245816228839516 19.096451712756828 14.28153942403515 7 25.010792421728002 33.067457697298856 24.12045844770688 17.80129143326626 8 23.755340969867234 34.591591715137824 22.522289549624734 19.130777765370205 9 25.175870208993818 16.816803090013288 22.344838169369556 35.66248853162333 10-11 23.387576338142573 26.729404882017345 29.84304944813234 20.039969331707745 12-13 24.380476432259993 25.19925356442578 29.111444529159474 21.308825474154748 14-15 22.07373265869383 26.948648603632748 26.995660652368514 23.981958085304907 16-17 21.336741067965526 29.13985457247492 28.021377274601626 21.502027084957927 18-19 21.25237281514839 28.266531973071736 29.664827204186867 20.81626800759301 20-21 22.11595817345147 27.61297134430417 29.358596341344533 20.912474140899825 22-23 22.056717937145997 27.541033500657285 28.966647927559823 21.435600634636888 24-25 21.654385745805754 27.72299727934136 29.23954611610266 21.383070858750227 26-27 21.533116336188733 27.773452238234064 29.379184306460004 21.314247119117198 28-29 21.543424162046428 27.680901218672354 29.439602067769037 21.33607255151218 30-31 21.893200650138077 27.54202617661214 29.18661888365101 21.37815428959877 32-33 21.687710370375935 27.789230799871877 28.9262960480326 21.596762781719583 34-35 21.880687163668508 27.848010739748858 28.92662271015381 21.344679386428822 36-37 21.92047706511298 27.663215960559377 28.957879788983398 21.458427185344245 38-39 21.696529908300977 27.847423846448216 29.07463786003393 21.38140838521688 40-41 21.894162432860313 27.678946786690773 28.99012473749421 21.436766042954705 42-43 21.844095542011658 27.73599114903098 28.717162922902894 21.70275038605447 44-45 21.909837165717512 27.87350438068404 28.588629129516 21.628029324082444 46-47 21.90129543234334 27.896754014478525 28.535073529855975 21.666877023322158 48-49 21.845802423996385 27.895423820510807 28.565314143157828 21.693459612334983 50-51 21.81256560762148 28.12097159611454 28.35962046730832 21.706842328955656 52-53 21.975002852866066 28.127496257807017 28.15004165429336 21.747459235033553 54-55 21.990642342372343 28.105000871709784 28.133731381486974 21.7706254044309 56-57 21.968169739618837 28.202884234673675 28.106666884365296 21.72227914134219 58-59 21.837230962376566 28.108902332210395 28.26450274721014 21.789363958202898 60-61 21.947315302678412 28.09834742396593 28.286075594750077 21.668261678605585 62-63 21.82959673760558 28.091150221286505 28.318322137450192 21.760930903657723 64-65 21.89953240048569 28.09004964693265 28.177655157278426 21.832762795303235 66-67 21.86369675325322 28.113730428795375 28.12899667457083 21.89357614338058 68-69 21.738943722536572 28.128363149894657 28.23660442752382 21.896088700044952 70-71 21.865608236866063 28.007111127945443 28.186930126914923 21.940350508273568 72-73 21.83134924457468 28.042542450530572 28.165418046418434 21.96069025847631 74-75 21.867238963923008 28.13571567791727 28.01607716936823 21.98096818879149 76-77 21.83170733698536 28.142869971997484 28.043628407448836 21.98179428356832 78-79 21.814516003937694 28.17803020345343 27.996200116942088 22.01125367566679 80-81 21.837794660775884 28.207916140966592 27.959776453850864 21.994512744406652 82-83 21.871819893115752 28.215573852940757 27.96854608942379 21.94406016451971 84-85 21.889357055364577 28.109206116577045 27.91226270702978 22.0891741210286 86-87 21.81485871566764 28.135541908492538 28.009090034746503 22.040509341093315 88-89 21.828579657312133 28.121117267065408 27.937619614705543 22.11268346091691 90-91 21.763282732680192 28.149793913193555 28.028899668063328 22.05802368606292 92-93 21.890212854628434 28.13519735864426 27.993285563438818 21.981304223288493 94-95 21.828287175481687 28.035417255789625 28.030747810095274 22.105547758633413 96-97 21.797224378390865 28.074589015211803 27.99726078159141 22.130925824805924 98-99 21.752335895533413 28.030762725906627 27.945530189312223 22.271371189247738 100-101 21.77362495902719 28.09595615717423 27.9863033632573 22.14411552054128 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5510.0 1 3145.0 2 757.5 3 721.0 4 598.5 5 551.0 6 781.0 7 1383.0 8 2089.5 9 2344.0 10 1967.0 11 1625.0 12 1675.5 13 1817.0 14 2027.0 15 2252.5 16 2404.0 17 2549.5 18 2876.0 19 3156.0 20 3544.0 21 4296.5 22 5149.0 23 6213.5 24 7877.5 25 10260.0 26 13913.5 27 19966.0 28 28575.0 29 39202.5 30 52062.5 31 65415.0 32 76657.0 33 85860.0 34 92170.5 35 95707.5 36 98481.5 37 99958.5 38 99701.5 39 98632.0 40 98641.5 41 98906.0 42 98982.5 43 100559.5 44 102861.0 45 105253.5 46 106468.5 47 106497.5 48 106800.5 49 106090.0 50 102447.0 51 98322.0 52 94808.5 53 91033.5 54 86558.0 55 80667.0 56 73569.5 57 66141.0 58 60043.5 59 52686.0 60 43513.5 61 35136.0 62 27651.0 63 20885.5 64 15406.0 65 11344.5 66 8283.0 67 6229.0 68 4875.0 69 3691.0 70 2697.5 71 2040.0 72 1499.0 73 1136.5 74 855.0 75 626.5 76 463.0 77 328.0 78 252.0 79 196.5 80 144.5 81 112.0 82 95.5 83 69.5 84 52.0 85 39.0 86 28.5 87 25.5 88 23.0 89 15.5 90 9.5 91 8.0 92 7.0 93 7.5 94 6.5 95 6.0 96 5.0 97 3.5 98 2.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1494019031865231 2 0.15406561682015613 3 0.14783646084796098 4 0.15194574698668656 5 0.1408245837064848 6 0.11995201919232307 7 0.12693128295174588 8 0.11675590775109206 9 0.11271184837647323 10-11 0.10199835237193866 12-13 0.11972372551794944 14-15 0.1384438068165882 16-17 0.14883116900058901 18-19 0.14552091072217116 20-21 0.13695989793315952 22-23 0.13053506166864412 24-25 0.11572858621641066 26-27 0.11957696529870923 28-29 0.12376778489256825 30-31 0.11388593013039483 32-33 0.11326627587138063 34-35 0.12905115278521545 36-37 0.13053506166864412 38-39 0.11734294862805286 40-41 0.11983787235513624 42-43 0.11448927769838232 44-45 0.13152976982127212 46-47 0.1293609799147225 48-49 0.14289553346687425 50-51 0.13001324755579008 52-53 0.11468495799070258 54-55 0.10697189313507877 56-57 0.12681713611455905 58-59 0.11654392076774511 60-61 0.11587534643565085 62-63 0.11634824047542483 64-65 0.13710665815239972 66-67 0.12766508404794688 68-69 0.13324197237907445 70-71 0.12073474036160413 72-73 0.1232459707797142 74-75 0.10596087829142405 76-77 0.13048614159556407 78-79 0.1313177828379252 80-81 0.1317417568046191 82-83 0.13769369902936052 84-85 0.12800752455950734 86-87 0.13669899087673248 88-89 0.123572104600248 90-91 0.13193743709693936 92-93 0.1456187508683313 94-95 0.12275677004891355 96-97 0.10790137452360003 98-99 0.11181498037000535 100-101 0.15473419115225034 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3066226.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.38729106413608 #Duplication Level Percentage of deduplicated Percentage of total 1 59.12480931580766 29.20014166791337 2 20.698318038506343 20.44467715011542 3 9.037181333285483 13.389657147190427 4 4.426632713447376 8.744775930122083 5 2.357794695590507 5.822254645030226 6 1.3495079220103028 3.998912432260817 7 0.8210670452567287 2.8385194003084977 8 0.5448395969832482 2.152652140758262 9 0.36438754850333493 1.6196502526273082 >10 1.238826655776203 9.450851725338488 >50 0.02803120255321375 0.9246209561696028 >100 0.008074922215806976 0.6674438912112379 >500 1.3225251621398475E-4 0.044153572112851466 >1k 2.645050316073728E-4 0.2691669678037094 >5k 1.322525158036864E-4 0.432522121037652 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7045 0.2297612765660457 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6199 0.20217035534888816 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3108 0.1013623914218978 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.261338205337767E-5 2 0.0 0.0 0.0 0.0 3.261338205337767E-5 3 0.0 0.0 0.0 0.0 3.261338205337767E-5 4 0.0 0.0 0.0 0.0 3.261338205337767E-5 5 0.0 0.0 0.0 0.0 3.261338205337767E-5 6 0.0 0.0 0.0 0.0 3.261338205337767E-5 7 0.0 0.0 0.0 0.0 3.261338205337767E-5 8 0.0 0.0 0.0 0.0 3.261338205337767E-5 9 0.0 0.0 0.0 3.261338205337767E-5 3.261338205337767E-5 10-11 0.0 0.0 0.0 4.8920073080066506E-5 3.261338205337767E-5 12-13 0.0 0.0 0.0 6.522676410675534E-5 3.261338205337767E-5 14-15 0.0 0.0 0.0 6.522676410675534E-5 3.261338205337767E-5 16-17 0.0 0.0 0.0 1.1414683718682185E-4 3.261338205337767E-5 18-19 0.0 0.0 0.0 1.3045352821351068E-4 3.261338205337767E-5 20-21 0.0 0.0 0.0 1.6306691026688835E-4 3.261338205337767E-5 22-23 0.0 0.0 0.0 2.282936743736437E-4 6.522676410675534E-5 24-25 0.0 0.0 0.0 3.261338205337767E-4 9.784014616013301E-5 26-27 0.0 0.0 0.0 6.522676410675534E-4 1.3045352821351068E-4 28-29 0.0 0.0 0.0 0.0019241895411492827 1.3045352821351068E-4 30-31 0.0 0.0 0.0 0.00544643480291407 1.3045352821351068E-4 32-33 0.0 0.0 0.0 0.01386068737268551 1.3045352821351068E-4 34-35 0.0 0.0 0.0 0.028341029004385194 1.3045352821351068E-4 36-37 0.0 0.0 0.0 0.04924620690060028 1.3045352821351068E-4 38-39 0.0 0.0 0.0 0.07324965609188624 1.3045352821351068E-4 40-41 0.0 0.0 0.0 0.09567135625358339 1.3045352821351068E-4 42-43 0.0 0.0 0.0 0.12110979425521798 1.3045352821351068E-4 44-45 0.0 0.0 0.0 0.15727803495241383 1.3045352821351068E-4 46-47 0.0 0.0 0.0 0.1966097737087873 1.3045352821351068E-4 48-49 0.0 0.0 0.0 0.23329982851883718 1.3045352821351068E-4 50-51 0.0 0.0 0.0 0.26963113612629985 1.3045352821351068E-4 52-53 0.0 0.0 0.0 0.3068593117402305 1.3045352821351068E-4 54-55 0.0 0.0 0.0 0.3525343533059859 1.6306691026688835E-4 56-57 0.0 0.0 0.0 0.4064768872222726 1.6306691026688835E-4 58-59 0.0 0.0 0.0 0.4622620772245751 1.6306691026688835E-4 60-61 0.0 0.0 0.0 0.5196616296385198 1.6306691026688835E-4 62-63 0.0 0.0 0.0 0.5782189571153594 1.6306691026688835E-4 64-65 0.0 0.0 0.0 0.6462015520056251 1.6306691026688835E-4 66-67 0.0 0.0 0.0 0.7266750722223345 1.6306691026688835E-4 68-69 0.0 0.0 0.0 0.8153997780985485 1.6306691026688835E-4 70-71 0.0 0.0 0.0 0.9010099059886649 1.793736012935772E-4 72-73 0.0 0.0 0.0 0.9957354741627003 1.9568029232026603E-4 74-75 1.6306691026688835E-5 0.0 0.0 1.0991198952719075 1.9568029232026603E-4 76-77 3.261338205337767E-5 0.0 0.0 1.2202786096002056 1.9568029232026603E-4 78-79 3.261338205337767E-5 0.0 0.0 1.351955139640718 1.9568029232026603E-4 80-81 3.261338205337767E-5 0.0 0.0 1.4881812364776765 1.9568029232026603E-4 82-83 3.261338205337767E-5 0.0 0.0 1.6382191006142404 1.9568029232026603E-4 84-85 3.261338205337767E-5 0.0 0.0 1.823446803986399 1.9568029232026603E-4 86-87 3.261338205337767E-5 0.0 0.0 2.0398039805285064 1.9568029232026603E-4 88-89 3.261338205337767E-5 0.0 0.0 2.269989231061246 1.9568029232026603E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4715 0.0 53.199696 1 GTATCAA 8235 0.0 38.53628 1 ATCAACG 9160 0.0 33.866837 3 TCAACGC 9155 0.0 33.833443 4 TATCAAC 9370 0.0 33.564125 2 CAACGCA 9335 0.0 33.12908 5 AACGCAG 9635 0.0 32.385983 6 ACGCAGA 11005 0.0 28.095346 7 CGCAGAG 11350 0.0 26.910538 8 GCAGAGT 12650 0.0 23.093613 9 GAGTACT 7835 0.0 22.102081 12-13 GTGGTAT 2060 0.0 21.677917 1 CAGAGTA 11980 0.0 21.116219 10-11 TGGTATC 2310 0.0 19.53748 2 GTACTTT 8885 0.0 19.383347 14-15 GTACATG 6785 0.0 19.114798 1 AGAGTAC 10950 0.0 18.677227 10-11 TACATGG 6845 0.0 18.392017 2 CTATACG 190 1.8648764E-4 17.502552 4 ACATGGG 6955 0.0 17.349762 3 >>END_MODULE