FastQCFastQC Report
Fri 10 Feb 2017
SRR2031721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3066226
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89650.2923789701085308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79020.2577109449857904No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT43540.14199866546040638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40690.13270385157519374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52000.052.8172341
GTATCAA87650.038.490861
ATCAACG102700.032.381793
TCAACGC103150.032.2865754
CAACGCA106250.031.4257975
TATCAAC107950.030.9389742
AACGCAG110100.030.3268936
GTGGTAT22350.029.7647111
TGGTATC24100.027.2041552
ACGCAGA123650.026.7730967
CGCAGAG124950.026.3805078
GCAGAGT140800.022.8373629
GAGTACT92100.021.73691412-13
CAGAGTA134200.021.00526210-11
GTACATG66650.020.1048161
AGAGTAC122950.019.48913410-11
GTACTTT102850.019.25713514-15
TACATGG67600.018.2725812
ACATGGG67250.017.943813
AGTACTT101600.017.6007512-13
Produced by FastQC (version 0.11.5)