##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031721_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3066226
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76225170616908	34.0	31.0	34.0	31.0	34.0
2	32.92132217259915	34.0	31.0	34.0	31.0	34.0
3	32.97844646806856	34.0	31.0	34.0	31.0	34.0
4	36.36953473096895	37.0	37.0	37.0	35.0	37.0
5	36.335003029783195	37.0	37.0	37.0	35.0	37.0
6	36.36360887944985	37.0	37.0	37.0	35.0	37.0
7	36.311273206867334	37.0	37.0	37.0	35.0	37.0
8	36.30411880924628	37.0	37.0	37.0	35.0	37.0
9	38.15681035905377	39.0	39.0	39.0	37.0	39.0
10-11	38.12178097765788	39.0	39.0	39.0	37.0	39.0
12-13	38.07092872475806	39.0	39.0	39.0	35.0	39.0
14-15	39.56498412054428	41.0	39.5	41.0	37.0	41.0
16-17	39.54430919312536	41.0	39.0	41.0	37.0	41.0
18-19	39.55419675522939	41.0	39.5	41.0	37.0	41.0
20-21	39.54826128276259	41.0	40.0	41.0	37.0	41.0
22-23	39.45629594817864	41.0	39.0	41.0	36.0	41.0
24-25	39.39030798773476	41.0	39.0	41.0	36.0	41.0
26-27	39.25928812814189	41.0	39.0	41.0	36.0	41.0
28-29	39.15729955978457	41.0	39.0	41.0	36.0	41.0
30-31	38.991866711716625	40.0	39.0	41.0	35.0	41.0
32-33	38.79279364926134	40.0	38.0	41.0	35.0	41.0
34-35	38.652368742551914	40.0	38.0	41.0	35.0	41.0
36-37	38.554027165642715	40.0	38.0	41.0	35.0	41.0
38-39	38.629744513287676	40.0	38.0	41.0	35.0	41.0
40-41	38.552328334571556	40.0	38.0	41.0	35.0	41.0
42-43	38.4555535045362	40.0	38.0	41.0	35.0	41.0
44-45	38.2714830870262	40.0	38.0	41.0	34.0	41.0
46-47	38.06150068520716	40.0	38.0	41.0	33.5	41.0
48-49	37.822911455319996	40.0	38.0	41.0	33.0	41.0
50-51	37.66943190097534	40.0	38.0	41.0	33.0	41.0
52-53	37.72834128991144	40.0	38.0	41.0	33.0	41.0
54-55	37.65317576069083	40.0	37.5	41.0	33.0	41.0
56-57	37.47290056245039	40.0	37.0	41.0	33.0	41.0
58-59	37.29537662911997	40.0	37.0	41.0	33.0	41.0
60-61	37.04097382254276	40.0	36.0	41.0	32.5	41.0
62-63	36.77435991345713	39.0	36.0	41.0	32.0	41.0
64-65	36.46799518365574	39.0	35.0	41.0	31.5	41.0
66-67	36.1405421518179	38.5	35.0	41.0	31.0	41.0
68-69	35.77506224263965	37.5	35.0	40.0	31.0	41.0
70-71	35.43518057703509	37.0	35.0	40.0	31.0	41.0
72-73	35.05570121054352	36.5	35.0	39.0	30.0	41.0
74-75	34.67867583798454	36.0	35.0	39.0	30.0	41.0
76-77	33.74503526485002	35.0	33.5	37.0	28.5	39.0
78-79	33.95064453826952	35.0	34.0	37.0	29.0	39.0
80-81	33.72040107284981	35.0	34.0	37.0	29.0	39.0
82-83	33.43398774258649	35.0	34.0	36.5	29.0	39.0
84-85	33.130021074767484	35.0	34.0	36.0	29.0	37.0
86-87	32.87056498770802	35.0	34.0	36.0	29.0	37.0
88-89	32.63238668643473	35.0	34.0	35.0	29.0	37.0
90-91	32.42593158495166	35.0	34.0	35.0	29.0	36.0
92-93	32.24888445926686	35.0	34.0	35.0	28.0	36.0
94-95	32.064403928477546	35.0	34.0	35.0	27.0	36.0
96-97	31.898406053565523	35.0	34.0	35.0	27.0	36.0
98-99	31.69269437412637	35.0	33.5	35.0	26.0	35.0
100-101	30.986352930279764	34.5	32.0	35.0	22.5	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.21615489270435262
1101	2	0.2500481318663077
1101	3	0.24789994654231862
1101	4	0.18298643120703417
1101	5	0.1812376597091756
1101	6	0.13641720458186057
1101	7	0.21460480051692343
1101	8	0.17042250768371048
1101	9	0.15184473454208813
1101	10-11	0.19159771147933213
1101	12-13	0.20273525974414497
1101	14-15	0.10913468218884503
1101	16-17	0.19580293446325214
1101	18-19	0.21528223336612484
1101	20-21	0.18793158279465416
1101	22-23	0.12188042155404588
1101	24-25	0.16095437094715237
1101	26-27	0.23057887857563486
1101	28-29	0.12888344462261614
1101	30-31	0.21385693881981638
1101	32-33	0.1805144235198881
1101	34-35	-0.006204936678393835
1101	36-37	0.21678797606575273
1101	38-39	0.14682910006857952
1101	40-41	-0.07596824589246864
1101	42-43	0.046497408550294494
1101	44-45	0.11391318126897687
1101	46-47	0.21118993798012298
1101	48-49	0.057555350507023206
1101	50-51	0.10592511962497042
1101	52-53	0.10057495953962103
1101	54-55	0.185588592371694
1101	56-57	-0.017520582484301883
1101	58-59	0.1320427992176576
1101	60-61	0.11052537003344298
1101	62-63	0.2102786399274592
1101	64-65	0.15678570532253389
1101	66-67	-0.14223658901113367
1101	68-69	-0.10534540911794466
1101	70-71	0.02693276999959693
1101	72-73	-0.0431623375341772
1101	74-75	-0.06612035480973333
1101	76-77	-0.07250618285231525
1101	78-79	-0.051297946491693835
1101	80-81	-0.082838082440297
1101	82-83	-0.04812128388102366
1101	84-85	-0.13147164517348386
1101	86-87	-0.045399017126108276
1101	88-89	-0.06093345244715209
1101	90-91	-0.12372153083556015
1101	92-93	-0.1917461818102737
1101	94-95	-0.09913825395746656
1101	96-97	0.036752263252850526
1101	98-99	-0.19937239832585973
1101	100-101	-0.4970949213689586
1102	1	0.15524695040164005
1102	2	0.12136147982837997
1102	3	0.18051274245011228
1102	4	0.07185285637213923
1102	5	0.09485287919350327
1102	6	0.15215447861262987
1102	7	0.14387633066505146
1102	8	0.09204887223748415
1102	9	0.12163788175259782
1102	10-11	0.23362537244923232
1102	12-13	0.22261881343773382
1102	14-15	0.2350608279379358
1102	16-17	0.2323412948125636
1102	18-19	0.23355027817441965
1102	20-21	0.24165985971254145
1102	22-23	0.16457982790620207
1102	24-25	0.20406446671883316
1102	26-27	0.22416679915448157
1102	28-29	0.1958385830872018
1102	30-31	0.13351183755766982
1102	32-33	0.25736964348028835
1102	34-35	0.273001071085325
1102	36-37	0.19254156178764248
1102	38-39	0.04372420975593627
1102	40-41	0.15743105353166698
1102	42-43	0.18704895230402485
1102	44-45	0.12559547475287758
1102	46-47	0.2066770524331929
1102	48-49	0.1438547827925234
1102	50-51	0.19329281420797884
1102	52-53	0.20274501267790157
1102	54-55	0.31690514441594786
1102	56-57	0.36326978597575277
1102	58-59	0.27782454515742927
1102	60-61	0.3892136507348525
1102	62-63	0.36673463062152933
1102	64-65	0.3706051028297992
1102	66-67	0.4030336782963886
1102	68-69	0.3556495100784218
1102	70-71	0.3778179016175258
1102	72-73	0.3803317438401166
1102	74-75	0.297214969460299
1102	76-77	0.31297481471658983
1102	78-79	0.35781777693869543
1102	80-81	0.23698146791078045
1102	82-83	0.2527552659332102
1102	84-85	0.3180973384340149
1102	86-87	0.41848889386868393
1102	88-89	0.2652395297797412
1102	90-91	0.23979537375169713
1102	92-93	0.2475189996823488
1102	94-95	0.34519395016918963
1102	96-97	0.24165676406436987
1102	98-99	0.19395648645935282
1102	100-101	0.25069941413947916
1103	1	0.21477262598335045
1103	2	0.16470298256677296
1103	3	0.1882255526036687
1103	4	0.1488778336553409
1103	5	0.07790278779595639
1103	6	0.11327958188420695
1103	7	0.13541233399331531
1103	8	0.11896621726530299
1103	9	0.14867264110773704
1103	10-11	0.16940165057120993
1103	12-13	0.12682905255636
1103	14-15	0.19056934246548707
1103	16-17	0.16747628740529308
1103	18-19	0.23236538051492772
1103	20-21	0.2640897528993875
1103	22-23	0.16672245522929785
1103	24-25	0.13365306871931892
1103	26-27	0.20204568944324564
1103	28-29	0.24763163392686494
1103	30-31	0.19336876296996053
1103	32-33	0.15308391740894223
1103	34-35	0.15520569965533326
1103	36-37	0.16055175576730818
1103	38-39	0.011734624716140729
1103	40-41	0.09373599076729988
1103	42-43	0.15266924173550223
1103	44-45	0.21881353776119994
1103	46-47	0.1093103744130417
1103	48-49	0.1354441160912856
1103	50-51	0.021874157439668807
1103	52-53	0.028467530653934148
1103	54-55	0.15963763951607035
1103	56-57	0.12262941677914085
1103	58-59	0.23235779889848374
1103	60-61	0.08624608832110425
1103	62-63	-0.05130950596919348
1103	64-65	0.13207001524489925
1103	66-67	0.21209846213027816
1103	68-69	0.11694971833048129
1103	70-71	0.11486660042517371
1103	72-73	0.1422492244026614
1103	74-75	0.23664331931905025
1103	76-77	0.22421068816722567
1103	78-79	0.17573629345802289
1103	80-81	0.21238051457126517
1103	82-83	0.1807380760042676
1103	84-85	0.06539364151796434
1103	86-87	0.08228357466082059
1103	88-89	0.1484839454541529
1103	90-91	0.08184430237910334
1103	92-93	0.1127563740328128
1103	94-95	0.15178949744075965
1103	96-97	0.060692116236634064
1103	98-99	0.28565744439526597
1103	100-101	0.2114349287177113
1104	1	0.1008330476964403
1104	2	0.07359833359841161
1104	3	0.13884011607044755
1104	4	0.05293000050221508
1104	5	0.11828680115294077
1104	6	0.1160127019261239
1104	7	0.08951850826912278
1104	8	0.1609091192570844
1104	9	0.08528523743856198
1104	10-11	0.09899772162788878
1104	12-13	0.16295630360598068
1104	14-15	0.24170627377780818
1104	16-17	0.10718640128159507
1104	18-19	0.14613028112572124
1104	20-21	0.19129698045883003
1104	22-23	0.14154045074885602
1104	24-25	0.1889189595588192
1104	26-27	0.22428795244887567
1104	28-29	0.2829305952968397
1104	30-31	0.19400500156623224
1104	32-33	0.250634766541161
1104	34-35	0.12942464566122425
1104	36-37	0.15288067488604185
1104	38-39	0.1938009944622081
1104	40-41	0.2821804269475052
1104	42-43	0.2095244107959573
1104	44-45	0.13602273023635547
1104	46-47	0.02449761866871114
1104	48-49	-0.052788980638069916
1104	50-51	0.12736085151894372
1104	52-53	0.12035093011352416
1104	54-55	0.17947482349212862
1104	56-57	0.12364365187187332
1104	58-59	0.03384885908707247
1104	60-61	0.14990325160449203
1104	62-63	0.09559639011007448
1104	64-65	0.09888375455719967
1104	66-67	0.07245498331917588
1104	68-69	0.09897545761614168
1104	70-71	0.12954782337983062
1104	72-73	0.025182155279821927
1104	74-75	0.010957208497806903
1104	76-77	0.06740083983206802
1104	78-79	0.1590539370689399
1104	80-81	0.21114175733049478
1104	82-83	0.22019583142665766
1104	84-85	0.27962093564031676
1104	86-87	0.2274088352619259
1104	88-89	0.25322269492406946
1104	90-91	0.282826177419345
1104	92-93	0.2980720704899724
1104	94-95	0.3718113501897591
1104	96-97	0.37756002949614853
1104	98-99	0.473565969243122
1104	100-101	0.5268015409143949
1105	1	0.12782051249654103
1105	2	0.1265700594442265
1105	3	0.07066680102830958
1105	4	0.06155613798173931
1105	5	0.04387942235221942
1105	6	0.007365394725169949
1105	7	0.06364743187263144
1105	8	0.03273746006444611
1105	9	0.06694591824422247
1105	10-11	0.0401127528637133
1105	12-13	0.10783917845414948
1105	14-15	0.14488505941132246
1105	16-17	0.19396955206974553
1105	18-19	0.13401454562985293
1105	20-21	0.11472376623486724
1105	22-23	0.13678302746027526
1105	24-25	0.13092780892157663
1105	26-27	0.1424516520358523
1105	28-29	0.22809092973652412
1105	30-31	0.22162244897991457
1105	32-33	0.09644190409090214
1105	34-35	0.10683867510810074
1105	36-37	0.17427453860124587
1105	38-39	0.19018013369294806
1105	40-41	0.27117059979514124
1105	42-43	0.13038855987272058
1105	44-45	0.18482260615244428
1105	46-47	0.23690944206292386
1105	48-49	0.2507068792141851
1105	50-51	0.2677098165606111
1105	52-53	0.3297451511987788
1105	54-55	0.33422352213066375
1105	56-57	0.2072819986214327
1105	58-59	0.20971789187184697
1105	60-61	0.20905257988503223
1105	62-63	0.21045797995615345
1105	64-65	0.23476153295213464
1105	66-67	0.3811564200306137
1105	68-69	0.27401669665268713
1105	70-71	0.29948789315607627
1105	72-73	0.20056307926479633
1105	74-75	0.30807189074761965
1105	76-77	0.21082151049304088
1105	78-79	0.3050895137290084
1105	80-81	0.2781844048871207
1105	82-83	0.21881403550067802
1105	84-85	0.34101718880921794
1105	86-87	0.32193282045150795
1105	88-89	0.22560642651715312
1105	90-91	0.26853045841910017
1105	92-93	0.23863454055629774
1105	94-95	0.22058329674563737
1105	96-97	0.31222550073729494
1105	98-99	0.36633674517629444
1105	100-101	0.24723833933987294
1106	1	0.1294098209188519
1106	2	0.08754179499991466
1106	3	0.052914353825052274
1106	4	0.08550831870078213
1106	5	0.10679694341089885
1106	6	0.08342303877579127
1106	7	0.06687669185983935
1106	8	0.05810258446172867
1106	9	0.08848077515962416
1106	10-11	0.05712498723409709
1106	12-13	0.041524452086640906
1106	14-15	0.08156460884941197
1106	16-17	0.1041181719681532
1106	18-19	0.11864017379473779
1106	20-21	0.14395365875493127
1106	22-23	0.13698661289348024
1106	24-25	0.14134016564580065
1106	26-27	0.11990981953399427
1106	28-29	0.07610155314854694
1106	30-31	0.14073819692089273
1106	32-33	0.15307372489925797
1106	34-35	0.18807540343954798
1106	36-37	0.15341988239475057
1106	38-39	0.15199710329845573
1106	40-41	0.269253756848137
1106	42-43	0.2893520036266679
1106	44-45	0.29334807534849716
1106	46-47	0.21102706126802673
1106	48-49	0.20514545946407026
1106	50-51	0.15373096716267298
1106	52-53	0.1948137144173714
1106	54-55	0.1805990050763313
1106	56-57	0.27346682532819244
1106	58-59	0.25796232201241764
1106	60-61	0.14809450531706148
1106	62-63	0.15256596784839616
1106	64-65	0.20462740712114424
1106	66-67	0.2729326573565416
1106	68-69	0.3089925601309247
1106	70-71	0.21689570111322354
1106	72-73	0.25634270259122616
1106	74-75	0.2639490527666837
1106	76-77	0.20588582981628178
1106	78-79	0.17524866233674885
1106	80-81	0.20725482215116386
1106	82-83	0.14962765303657477
1106	84-85	0.20140993397647122
1106	86-87	0.19336804964463994
1106	88-89	0.24650229358093156
1106	90-91	0.2933707299473767
1106	92-93	0.14041146916111558
1106	94-95	0.2720812955818559
1106	96-97	0.34088863318785556
1106	98-99	0.29581635903246095
1106	100-101	0.3082162280373879
1107	1	0.15842769814331348
1107	2	0.1421293529681975
1107	3	0.17178392539295828
1107	4	0.15665206204266724
1107	5	0.06874665157835125
1107	6	0.13170691836793935
1107	7	0.15795178615173455
1107	8	0.14894495889752335
1107	9	0.14952138087159028
1107	10-11	0.17228850501633275
1107	12-13	0.1820466944952699
1107	14-15	0.2485325720968703
1107	16-17	0.23393602865928642
1107	18-19	0.26175627017647685
1107	20-21	0.2911397727223033
1107	22-23	0.3138677275779287
1107	24-25	0.2936607358583956
1107	26-27	0.2897390198297174
1107	28-29	0.2734666457522579
1107	30-31	0.31109779738892485
1107	32-33	0.31751841748413057
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2316	86-87	-0.4241167998316797
2316	88-89	-0.5278581900259738
2316	90-91	-0.5467243141276654
2316	92-93	-0.4931480214460251
2316	94-95	-0.402441701822319
2316	96-97	-0.44273354625741135
2316	98-99	-0.39714658503613975
2316	100-101	-0.21251579858688885
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	5.0
4	4.0
5	25.0
6	54.0
7	141.0
8	403.0
9	854.0
10	1617.0
11	2576.0
12	3987.0
13	5546.0
14	7058.0
15	8657.0
16	11023.0
17	16044.0
18	16438.0
19	10061.0
20	7666.0
21	7707.0
22	8217.0
23	8795.0
24	9937.0
25	11819.0
26	14114.0
27	16021.0
28	20430.0
29	26021.0
30	33975.0
31	43805.0
32	56620.0
33	75085.0
34	106941.0
35	168723.0
36	300662.0
37	648663.0
38	1031471.0
39	384643.0
40	416.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.0481290888262	20.630387073372493	14.752882891581356	23.56860094621995
2	15.670436556209491	22.182644071245893	39.399183230459855	22.747736142084765
3	17.932556536905985	26.31173677500658	31.642794966901842	24.112911721185593
4	13.22763991080364	19.350527597243733	37.85133461812972	29.570497873822905
5	13.524345563569026	37.11177845338211	35.36621240573917	13.997663577309696
6	29.07352556530406	37.60652345913185	19.091482493462646	14.22846848210145
7	24.819403396879423	33.203977788982286	24.173658432222545	17.80296038191575
8	23.677119690459868	34.754809332384504	22.50988674676948	19.05818423038615
9	24.84565716943239	16.582828532534784	22.41361204294791	36.15790225508492
10-11	23.193727923794256	26.751033151345194	29.953357964050586	20.101880960809957
12-13	24.22010778726284	25.13564725354467	29.300361193324036	21.343883765868455
14-15	21.811700940913145	26.898789341351016	27.21928130809773	24.070228409638112
16-17	21.052291708521082	29.21424480722618	28.196416131582446	21.537047352670292
18-19	20.981445890560398	28.06035507551478	30.103244351923752	20.854954682001072
20-21	21.788951629788542	27.332900445042213	29.91865244114426	20.959495484024988
22-23	21.614894009769664	27.440638752655545	29.49604823649659	21.448419001078197
24-25	21.367032306163996	27.63377520117565	29.764684664470266	21.234507828190093
26-27	21.221583070740568	27.6633787943323	29.912109967393057	21.202928167534075
28-29	21.178115928392057	27.47780414353095	30.03338327261745	21.310696655459545
30-31	21.512416980557035	27.336533903502502	29.85208682534498	21.298962290595487
32-33	21.367331540572508	27.571989685673294	29.56338267257688	21.49729610117733
34-35	21.536583042877822	27.67445759349536	29.64802206483371	21.1409372987931
36-37	21.566034262075146	27.587426574103958	29.48335206776423	21.36318709605667
38-39	21.33825099650189	27.591231044287017	29.750090176001375	21.320427783209716
40-41	21.633842384742678	27.465115095886606	29.558535476510865	21.342507042859854
42-43	21.61500815660685	27.536244882145024	29.334823982315722	21.513922978932406
44-45	21.59260276313618	27.71142766384474	29.243767419622692	21.452202153396392
46-47	21.734829722270963	27.6668614772688	28.95769913894149	21.640609661518752
48-49	21.65288859986185	27.69799095043875	29.02957903298713	21.61954141671227
50-51	21.60758861218971	27.88709964627526	28.841383511848118	21.663928229686917
52-53	21.72173544937653	28.022494101869853	28.640517691781366	21.615252756972254
54-55	21.71862087139043	27.990174240254955	28.56642008775609	21.72478480059852
56-57	21.746548564855576	28.131257186158415	28.468936309131966	21.653257939854047
58-59	21.54834217430937	28.13398603816106	28.75326746493641	21.564404322593163
60-61	21.759734807451494	28.16015657423097	28.546553258410917	21.53355535990662
62-63	21.55060223515874	28.253974673767544	28.550248669890067	21.645174421183647
64-65	21.63138510860871	28.143325503254466	28.49125170102416	21.734037687112657
66-67	21.56496690893009	28.204380571439007	28.450574087337465	21.78007843229344
68-69	21.506505925388634	28.167529209478538	28.550618239029813	21.77534662610302
70-71	21.585325524374948	27.988596389954807	28.52270644460838	21.903371641061867
72-73	21.596786576969315	28.020419998082204	28.44189556864385	21.940897856304638
74-75	21.597913792099682	28.159224198367834	28.252194238075383	21.990667771457105
76-77	21.53287653205874	28.098822752049664	28.31095992027856	22.057340795613037
78-79	21.55101296696204	28.07964736986034	28.269686197855304	22.099653465322316
80-81	21.52666024358567	28.146010503498665	28.247917662798685	22.07941159011698
82-83	21.58823529411765	28.023500057078554	28.257391673054027	22.130872975749767
84-85	21.634618285376405	28.010028140956887	28.17559291774775	22.179760655918955
86-87	21.521745688653564	28.04488969590036	28.213356510905662	22.22000810454042
88-89	21.570875865440527	27.97728011719995	28.15412693116884	22.297717086190687
90-91	21.554118972313198	27.946896282270128	28.20984167507548	22.289143070341193
92-93	21.52913712166031	27.886137551504685	28.296658498101575	22.288066828733434
94-95	21.487147392266586	27.830189294592117	28.259087881976086	22.42357543116522
96-97	21.526160017611232	27.849750099878516	28.195142235158865	22.428947647351386
98-99	21.404417713053952	27.81526597286011	28.19159197247299	22.588724341612952
100-101	21.380897170597436	27.779430205841443	28.178931868325403	22.66074075523572
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2588.0
1	1555.5
2	576.0
3	615.5
4	476.0
5	468.0
6	741.5
7	1407.5
8	2269.0
9	2561.5
10	2123.0
11	1733.0
12	1747.5
13	1907.5
14	2136.0
15	2351.0
16	2561.5
17	2788.5
18	3133.5
19	3458.5
20	3846.5
21	4537.0
22	5408.0
23	6566.5
24	8313.0
25	10728.0
26	14562.0
27	20730.0
28	29438.5
29	40734.5
30	53702.0
31	66262.0
32	77490.0
33	86490.5
34	92894.5
35	97105.0
36	99307.5
37	99872.5
38	99654.5
39	100100.0
40	100819.5
41	101219.0
42	101645.5
43	103228.5
44	105555.0
45	107918.0
46	108993.5
47	108931.0
48	109170.5
49	107712.0
50	103773.5
51	98535.5
52	93308.5
53	88882.0
54	84122.5
55	77842.0
56	69895.5
57	62338.0
58	56611.5
59	49882.5
60	40916.5
61	32470.5
62	25502.0
63	19206.0
64	14125.5
65	10384.0
66	7669.0
67	5689.5
68	4332.5
69	3310.5
70	2437.5
71	1813.0
72	1299.5
73	918.0
74	680.0
75	471.0
76	311.0
77	219.0
78	159.0
79	102.5
80	68.5
81	38.0
82	18.0
83	10.5
84	9.5
85	8.0
86	6.0
87	5.0
88	4.5
89	3.5
90	2.0
91	1.5
92	0.5
93	1.0
94	1.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01806781365757123
2	0.0
3	0.0014023754282952397
4	0.020546430693627934
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.6306691026688835E-5
12-13	3.261338205337767E-5
14-15	8.153345513344418E-5
16-17	0.0013697620462418622
18-19	1.6306691026688835E-4
20-21	0.0
22-23	0.0
24-25	0.0
26-27	2.6090705642702137E-4
28-29	0.005527968258047515
30-31	4.89200730800665E-5
32-33	1.793736012935772E-4
34-35	0.01147991048278894
36-37	0.0040603660656455196
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	4.89200730800665E-5
58-59	3.587472025871544E-4
60-61	0.0024949237270833918
62-63	0.010240601964760588
64-65	0.0019078828501225937
66-67	0.0046311002515796295
68-69	0.003196111441231012
70-71	1.3045352821351068E-4
72-73	0.006914036995316066
74-75	0.00601716898884818
76-77	0.0016143624116421946
78-79	3.4244051156046555E-4
80-81	0.006522676410675534
82-83	0.009001293446732237
84-85	0.001777429321909083
86-87	0.0014186821193219285
88-89	4.2397396669390967E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	3.261338205337767E-5
98-99	4.89200730800665E-5
100-101	0.0010110148436547077
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3066226.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.48859927291354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.187942062306306	27.157752638394612
2	21.389177392898244	20.314841479772127
3	9.564461406102735	13.626086249269797
4	4.587359663798148	8.713891391793506
5	2.5410134123530175	6.033458384316552
6	1.44747259861272	4.124306771642536
7	0.8817366127501651	2.931070566700412
8	0.5765669367798962	2.1904284971801413
9	0.4033512601274037	1.7239127722573486
>10	1.3792673155075958	10.119735181780483
>50	0.031610156320934904	1.0081978571039056
>100	0.008872188270834965	0.7429574015558107
>500	4.8135054177569735E-4	0.1494630006825062
>1k	5.501149042519596E-4	0.6131019958687801
>5k	1.375287260629899E-4	0.5507958116814228
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8965	0.2923789701085308	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7902	0.2577109449857904	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	4354	0.14199866546040638	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4069	0.13270385157519374	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.261338205337767E-5
7	0.0	0.0	0.0	0.0	3.261338205337767E-5
8	0.0	0.0	0.0	0.0	3.261338205337767E-5
9	0.0	0.0	0.0	3.261338205337767E-5	3.261338205337767E-5
10-11	0.0	0.0	0.0	3.261338205337767E-5	3.261338205337767E-5
12-13	0.0	0.0	0.0	3.261338205337767E-5	3.261338205337767E-5
14-15	0.0	0.0	0.0	3.261338205337767E-5	3.261338205337767E-5
16-17	0.0	0.0	0.0	6.522676410675534E-5	3.261338205337767E-5
18-19	0.0	0.0	0.0	6.522676410675534E-5	8.153345513344418E-5
20-21	0.0	0.0	0.0	9.784014616013301E-5	9.784014616013301E-5
22-23	0.0	0.0	0.0	1.6306691026688835E-4	9.784014616013301E-5
24-25	0.0	0.0	0.0	2.6090705642702137E-4	9.784014616013301E-5
26-27	0.0	0.0	0.0	5.544274949074204E-4	9.784014616013301E-5
28-29	0.0	0.0	0.0	0.0018263493949891496	9.784014616013301E-5
30-31	0.0	0.0	0.0	0.005479048184967449	9.784014616013301E-5
32-33	0.0	0.0	0.0	0.013730233844471999	9.784014616013301E-5
34-35	0.0	0.0	0.0	0.028014895183851418	9.784014616013301E-5
36-37	0.0	0.0	0.0	0.04877331286082631	1.1414683718682185E-4
38-39	0.0	0.0	0.0	0.072581081759792	1.3045352821351068E-4
40-41	0.0	0.0	0.0	0.09485602170224895	1.3045352821351068E-4
42-43	0.0	0.0	0.0	0.12039229985004368	1.3045352821351068E-4
44-45	0.0	0.0	0.0	0.15664207400237296	1.6306691026688835E-4
46-47	0.0	0.0	0.0	0.19589227930361297	1.9568029232026603E-4
48-49	0.0	0.0	0.0	0.23295738800727672	2.282936743736437E-4
50-51	0.0	0.0	0.0	0.26935392237884614	2.6090705642702137E-4
52-53	0.0	0.0	0.0	0.306728858212017	2.6090705642702137E-4
54-55	0.0	0.0	0.0	0.3524854332329058	2.6090705642702137E-4
56-57	0.0	0.0	0.0	0.4068682478069131	2.6090705642702137E-4
58-59	0.0	0.0	0.0	0.46302849170282945	2.6090705642702137E-4
60-61	0.0	0.0	0.0	0.5207052578642279	2.6090705642702137E-4
62-63	0.0	0.0	0.0	0.5798985462911084	2.6090705642702137E-4
64-65	0.0	0.0	0.0	0.6482561950749879	2.6090705642702137E-4
66-67	0.0	0.0	0.0	0.7295124364609784	2.6090705642702137E-4
68-69	0.0	0.0	0.0	0.8190524768885268	2.6090705642702137E-4
70-71	0.0	0.0	0.0	0.9049724319081502	2.6090705642702137E-4
72-73	0.0	0.0	0.0	1.0000078272116928	2.6090705642702137E-4
74-75	0.0	0.0	0.0	1.1043706497825014	2.6090705642702137E-4
76-77	0.0	0.0	0.0	1.2260348715326268	2.6090705642702137E-4
78-79	0.0	0.0	0.0	1.3580049220116195	2.6090705642702137E-4
80-81	0.0	0.0	0.0	1.4953398738383927	2.6090705642702137E-4
82-83	0.0	0.0	0.0	1.6469268736224922	2.6090705642702137E-4
84-85	0.0	0.0	0.0	1.8339156996255332	2.6090705642702137E-4
86-87	0.0	0.0	3.261338205337767E-5	2.0510719040279484	2.6090705642702137E-4
88-89	0.0	0.0	3.261338205337767E-5	2.2824475430056363	2.6090705642702137E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5200	0.0	52.817234	1
GTATCAA	8765	0.0	38.49086	1
ATCAACG	10270	0.0	32.38179	3
TCAACGC	10315	0.0	32.286575	4
CAACGCA	10625	0.0	31.425797	5
TATCAAC	10795	0.0	30.938974	2
AACGCAG	11010	0.0	30.326893	6
GTGGTAT	2235	0.0	29.764711	1
TGGTATC	2410	0.0	27.204155	2
ACGCAGA	12365	0.0	26.773096	7
CGCAGAG	12495	0.0	26.380507	8
GCAGAGT	14080	0.0	22.837362	9
GAGTACT	9210	0.0	21.736914	12-13
CAGAGTA	13420	0.0	21.005262	10-11
GTACATG	6665	0.0	20.104816	1
AGAGTAC	12295	0.0	19.489134	10-11
GTACTTT	10285	0.0	19.257135	14-15
TACATGG	6760	0.0	18.272581	2
ACATGGG	6725	0.0	17.94381	3
AGTACTT	10160	0.0	17.60075	12-13
>>END_MODULE