FastQCFastQC Report
Fri 10 Feb 2017
SRR2031718_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031718_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258406
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37740.2999032108874243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36040.2863940572438466No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18210.14470687520561726No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13330.10592765768758254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23800.057.682581
GTATCAA40250.038.2386861
GTGGTAT9700.035.749911
TATCAAC49350.031.6688692
TCAACGC49050.031.5682544
ATCAACG49050.031.471423
CAACGCA50900.030.5141945
AACGCAG52450.029.7006366
GCGTCAG3900.029.2328011
TGGTATC11750.029.1084082
CGTCAGA4300.026.5134722
ACGCAGA61300.025.568677
CGCAGAG61300.025.2607568
GAGTACT41200.022.94638312-13
GCAGAGT67300.022.4440569
CAGAGTA64650.021.16198710-11
GTACTTT47500.019.80295414-15
GTACATG32100.019.6820551
AGAGTAC59050.019.1867310-11
TATGCCG1250.006033185418.9990545
Produced by FastQC (version 0.11.5)