##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031718_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1258406
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.822685206523172	33.0	31.0	34.0	30.0	34.0
2	32.13652032809761	34.0	31.0	34.0	31.0	34.0
3	32.23762283396614	34.0	31.0	34.0	31.0	34.0
4	35.65382475925894	37.0	35.0	37.0	35.0	37.0
5	35.64801661784829	37.0	37.0	37.0	35.0	37.0
6	35.67339634426409	37.0	37.0	37.0	35.0	37.0
7	35.65282984982589	37.0	37.0	37.0	35.0	37.0
8	35.64657113840843	37.0	37.0	37.0	35.0	37.0
9	37.40242974048121	39.0	38.0	39.0	35.0	39.0
10-11	37.33206055915181	39.0	38.0	39.0	35.0	39.0
12-13	37.28385195239056	39.0	38.0	39.0	35.0	39.0
14-15	38.6527674693223	41.0	39.0	41.0	35.0	41.0
16-17	38.572652625623206	41.0	39.0	41.0	35.0	41.0
18-19	38.58379886936331	41.0	39.0	41.0	35.0	41.0
20-21	38.591447831621906	41.0	39.0	41.0	35.0	41.0
22-23	38.485441502980756	41.0	39.0	41.0	34.5	41.0
24-25	38.41021856221283	41.0	38.5	41.0	34.0	41.0
26-27	38.2856983358312	40.0	38.0	41.0	34.0	41.0
28-29	38.1660072345491	40.0	38.0	41.0	34.0	41.0
30-31	37.97942277770449	40.0	38.0	41.0	34.0	41.0
32-33	37.75381275995188	40.0	38.0	41.0	33.0	41.0
34-35	37.56968259846187	40.0	38.0	41.0	33.0	41.0
36-37	37.394375503613304	40.0	38.0	41.0	32.5	41.0
38-39	37.19235723605895	40.0	37.0	41.0	31.5	41.0
40-41	37.07190366225208	40.0	37.0	41.0	31.0	41.0
42-43	37.01236842481679	40.0	37.0	41.0	31.0	41.0
44-45	36.84292986524222	40.0	37.0	41.0	31.0	41.0
46-47	36.71551748799672	40.0	37.0	41.0	30.5	41.0
48-49	36.71577098329156	40.0	37.0	41.0	30.0	41.0
50-51	36.1493671358846	39.5	36.0	40.5	29.5	41.0
52-53	36.15175428279903	39.5	36.0	40.5	30.0	41.0
54-55	36.35684349883901	40.0	36.0	41.0	30.0	41.0
56-57	36.221142461177074	40.0	36.0	41.0	29.5	41.0
58-59	36.01897161965216	39.5	35.5	41.0	29.0	41.0
60-61	35.770796150050145	39.0	35.0	41.0	28.0	41.0
62-63	35.477527920241954	39.0	35.0	41.0	28.0	41.0
64-65	35.193017992603345	38.5	35.0	41.0	28.0	41.0
66-67	34.89719255947604	38.0	35.0	40.5	27.5	41.0
68-69	34.55268848050629	37.0	35.0	40.0	26.5	41.0
70-71	34.1824681382638	37.0	34.5	39.5	26.0	41.0
72-73	33.817311741997415	36.0	34.0	39.0	26.0	41.0
74-75	33.418937131577565	36.0	34.0	39.0	26.0	40.5
76-77	33.03853843672074	35.0	34.0	37.5	25.0	39.5
78-79	32.651399866179915	35.0	34.0	37.0	24.5	39.0
80-81	32.30930995243189	35.0	34.0	37.0	24.0	39.0
82-83	31.991851993712682	35.0	33.5	36.0	23.0	38.0
84-85	31.67971823084124	35.0	33.0	36.0	21.0	37.0
86-87	31.410655623066006	35.0	33.0	35.5	20.0	37.0
88-89	31.140683134060072	35.0	33.0	35.0	18.5	36.5
90-91	30.907426935345192	35.0	33.0	35.0	17.5	36.0
92-93	30.69643501381907	35.0	33.0	35.0	12.5	36.0
94-95	30.446127084581605	35.0	32.0	35.0	4.5	36.0
96-97	30.186999664655126	35.0	32.0	35.0	2.0	35.0
98-99	29.92726473014274	35.0	32.0	35.0	2.0	35.0
100-101	29.3572881089251	34.5	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.004063324570033444
1101	2	0.0663313677020625
1101	3	0.26778276433309145
1101	4	0.251583473915467
1101	5	0.2595740967385183
1101	6	0.3155055855444573
1101	7	0.18777151726661145
1101	8	0.1535575341736859
1101	9	0.24960092868977313
1101	10-11	0.21817625627068082
1101	12-13	0.21940844900248635
1101	14-15	0.25739293101776894
1101	16-17	0.16645730852345508
1101	18-19	0.1759749693985384
1101	20-21	0.248241592902275
1101	22-23	0.25360679758485105
1101	24-25	0.018593824109807144
1101	26-27	-0.01706867106291554
1101	28-29	-0.06971331667482872
1101	30-31	-0.050843549600735116
1101	32-33	0.05424915661813401
1101	34-35	0.1492753394488986
1101	36-37	-0.08149118997821603
1101	38-39	0.14887373484972244
1101	40-41	-0.14671363345537003
1101	42-43	0.02800005257902427
1101	44-45	-0.07850764210412109
1101	46-47	-0.3745626067602288
1101	48-49	-0.34746754442694794
1101	50-51	-0.25277615624434446
1101	52-53	-0.22782642830264166
1101	54-55	-0.1219090112838046
1101	56-57	-0.19849804776109892
1101	58-59	-0.4204546862701193
1101	60-61	-0.5728868358684736
1101	62-63	-0.43085485408943214
1101	64-65	-0.1044401780833013
1101	66-67	-0.592226167136559
1101	68-69	-0.627012872571072
1101	70-71	-0.600550587193375
1101	72-73	-0.21817625326608692
1101	74-75	-0.10309167348703596
1101	76-77	-0.35286996888171274
1101	78-79	-0.520700170779719
1101	80-81	-0.6982231901600002
1101	82-83	-0.6206593011834727
1101	84-85	-0.6709456170890888
1101	86-87	-0.6849389006441697
1101	88-89	-0.8809911337896601
1101	90-91	-1.197076223351175
1101	92-93	-0.8845798836904244
1101	94-95	-0.9187321691176749
1101	96-97	-0.9298235883214687
1101	98-99	-0.9652906301685213
1101	100-101	-1.0282801817884284
1102	1	0.13869813699052713
1102	2	0.23948039864136916
1102	3	0.2096958517306362
1102	4	0.30535257194147647
1102	5	0.3177945971621554
1102	6	0.21960782963685688
1102	7	0.3309062927384261
1102	8	0.13305454857737686
1102	9	0.3352334670230732
1102	10-11	0.3877248209335846
1102	12-13	0.4023423303508835
1102	14-15	0.45802674118032627
1102	16-17	0.47923717958376244
1102	18-19	0.4322915688748026
1102	20-21	0.42244148420205363
1102	22-23	0.4643758974408314
1102	24-25	0.3463350736500246
1102	26-27	0.26915833307465675
1102	28-29	0.2898465389039302
1102	30-31	0.2707769072696067
1102	32-33	0.22432932761054758
1102	34-35	0.3646866160909781
1102	36-37	0.1939001033897867
1102	38-39	0.056433707546560186
1102	40-41	0.1369395176617374
1102	42-43	0.17109502619999972
1102	44-45	-0.1458272116219419
1102	46-47	-0.13363239633195434
1102	48-49	-0.054663170066298505
1102	50-51	-0.1716503769058093
1102	52-53	-0.0495036142477403
1102	54-55	-0.09703925940945624
1102	56-57	-0.01591506318957414
1102	58-59	0.016880273296692394
1102	60-61	-0.00800295662454431
1102	62-63	-0.017953198515442637
1102	64-65	-0.03837779806071495
1102	66-67	-0.010771908155525978
1102	68-69	-0.126764911615318
1102	70-71	-0.09967494647506925
1102	72-73	-0.02738448007990968
1102	74-75	-0.34600557156135636
1102	76-77	-0.11060845516700368
1102	78-79	-0.20489718902635445
1102	80-81	-0.06959230062980737
1102	82-83	0.04623031684685941
1102	84-85	-0.02235844554585853
1102	86-87	0.006625577439194785
1102	88-89	0.06406035242060071
1102	90-91	-0.05018268918471591
1102	92-93	0.07328459603991178
1102	94-95	-0.16345407770334575
1102	96-97	-0.2924431539963521
1102	98-99	-0.12440470501796241
1102	100-101	-0.1384420975475784
1103	1	0.09051197345989337
1103	2	0.2764280941341539
1103	3	0.27079575627006847
1103	4	0.3443174503363693
1103	5	0.292640652001424
1103	6	0.18650512126460228
1103	7	0.13756522429987683
1103	8	0.3222447104832966
1103	9	0.22718735802664725
1103	10-11	0.27445066058383105
1103	12-13	0.36430720703193487
1103	14-15	0.5851876719294253
1103	16-17	0.481216229673997
1103	18-19	0.4507821265050467
1103	20-21	0.2851860697712283
1103	22-23	0.32104322679006714
1103	24-25	0.4017313378341427
1103	26-27	0.3954597759516929
1103	28-29	0.13052250940541654
1103	30-31	0.4728812386428842
1103	32-33	0.4346167890278494
1103	34-35	0.39273351345115515
1103	36-37	0.3658516635787876
1103	38-39	0.4032306370411405
1103	40-41	0.428711656544003
1103	42-43	0.3390649209877168
1103	44-45	0.21276389666325457
1103	46-47	0.4619121487907236
1103	48-49	0.6406590907552783
1103	50-51	0.665380696622826
1103	52-53	0.6170180906060097
1103	54-55	0.46999865231779125
1103	56-57	0.3199823089883793
1103	58-59	0.44750640549843723
1103	60-61	0.534739640896909
1103	62-63	0.508927434643482
1103	64-65	0.49701330748233374
1103	66-67	0.33188996777316504
1103	68-69	0.3604164651762858
1103	70-71	0.3309036268264762
1103	72-73	0.16469006485424842
1103	74-75	0.36272144377917215
1103	76-77	0.33940157076330735
1103	78-79	0.3058952536501778
1103	80-81	0.3539582669064032
1103	82-83	0.2522271942408665
1103	84-85	0.22739947536754812
1103	86-87	0.29456301220637826
1103	88-89	0.40107642989962144
1103	90-91	0.18003837829407843
1103	92-93	-0.21778230005202914
1103	94-95	0.23225225337172262
1103	96-97	0.3842977159801855
1103	98-99	0.1946735263736521
1103	100-101	0.11692048979369929
1104	1	0.0045043835402047705
1104	2	0.06143198537205308
1104	3	0.12777471149274788
1104	4	0.027976658128473275
1104	5	-0.017242289934884525
1104	6	0.0935727325259279
1104	7	0.07125291434874015
1104	8	0.12056144922478751
1104	9	0.1553398624594493
1104	10-11	0.1569228402386713
1104	12-13	0.1528517235957736
1104	14-15	0.2179502903714905
1104	16-17	0.2293191509105199
1104	18-19	0.13718726454371222
1104	20-21	0.2675491343015892
1104	22-23	0.2813197052598895
1104	24-25	0.33046191173156103
1104	26-27	0.3601289404989103
1104	28-29	0.302434992657048
1104	30-31	0.2836740081612561
1104	32-33	0.17620128505237176
1104	34-35	0.28680800056995537
1104	36-37	0.30218338960354885
1104	38-39	0.12097107141561025
1104	40-41	0.18766471297096388
1104	42-43	0.0030713716171320016
1104	44-45	-0.00817978870008318
1104	46-47	0.14602867732513403
1104	48-49	0.2289693307283187
1104	50-51	-0.015462378177197422
1104	52-53	0.020476478430040856
1104	54-55	-0.004553855324267886
1104	56-57	0.10407757611985602
1104	58-59	-0.022439197402746913
1104	60-61	-0.06107186272554799
1104	62-63	0.010488342545500018
1104	64-65	-0.08629087499667065
1104	66-67	-0.019860688121063674
1104	68-69	0.01144543684646493
1104	70-71	0.1322587403944553
1104	72-73	-0.062098500522559164
1104	74-75	-0.10966801698350537
1104	76-77	0.07760189329478351
1104	78-79	0.00319706127058339
1104	80-81	-0.001024122233438618
1104	82-83	-0.04320040065318409
1104	84-85	-0.07362147313171974
1104	86-87	-0.03890363948369924
1104	88-89	0.13880900156659237
1104	90-91	0.14397141690960424
1104	92-93	-0.01713563247607297
1104	94-95	0.13746851794380888
1104	96-97	0.05048807373337638
1104	98-99	-0.04262659638086319
1104	100-101	-0.18546520007865297
1105	1	-0.09217916527972392
1105	2	0.12321833952498196
1105	3	0.017888181279381854
1105	4	0.06416444558396961
1105	5	0.15343938194120454
1105	6	0.2085937124147037
1105	7	0.20779437386647714
1105	8	0.18213761669160533
1105	9	0.07591876778236895
1105	10-11	0.25441805092949465
1105	12-13	0.13428161353861157
1105	14-15	0.2718441732408863
1105	16-17	0.1852295850119603
1105	18-19	0.07977023399296002
1105	20-21	0.17442130822137614
1105	22-23	-0.0577579552304428
1105	24-25	0.1252906212163296
1105	26-27	0.12434911686958117
1105	28-29	0.14077738754431124
1105	30-31	0.25620780478431016
1105	32-33	0.3645398019565036
1105	34-35	0.16593630752007726
1105	36-37	0.22747675902797226
1105	38-39	0.23490117464751847
1105	40-41	0.2604850925479596
1105	42-43	0.2939107998473034
1105	44-45	0.49974991122158485
1105	46-47	0.5387116987393554
1105	48-49	0.5244693190943721
1105	50-51	0.49590941957822565
1105	52-53	0.4455961142098701
1105	54-55	0.4280022009219877
1105	56-57	0.4567223606203328
1105	58-59	0.20889296736500285
1105	60-61	0.33864310551376775
1105	62-63	0.39697318350941657
1105	64-65	0.5624503316765654
1105	66-67	0.4352861711003939
1105	68-69	0.49725822333247294
1105	70-71	0.39044167261506857
1105	72-73	0.14664867824470917
1105	74-75	0.2580892678078115
1105	76-77	0.373186809491564
1105	78-79	0.3535999837186736
1105	80-81	0.4605105508138081
1105	82-83	0.39243361081013717
1105	84-85	0.5375614668448598
1105	86-87	0.5407551755597702
1105	88-89	0.6159541079396682
1105	90-91	0.2855755834146869
1105	92-93	0.49967697562270885
1105	94-95	0.43173569547012036
1105	96-97	0.3625563572604804
1105	98-99	0.4143584268482563
1105	100-101	0.38179725406419607
1106	1	-0.0017813047460748521
1106	2	0.13305576922142137
1106	3	0.12286081836992224
1106	4	0.10172752071387237
1106	5	0.1615119471604558
1106	6	0.20885205853391398
1106	7	0.1024417276587215
1106	8	0.16734504834816732
1106	9	0.22195041367995572
1106	10-11	0.17674486327634042
1106	12-13	0.2115901877008426
1106	14-15	0.33735031008772864
1106	16-17	0.361022103037989
1106	18-19	0.18120262804288245
1106	20-21	0.35853009296573646
1106	22-23	0.2548461060606755
1106	24-25	0.12229673515748374
1106	26-27	0.12293046623238979
1106	28-29	0.32828222214647695
1106	30-31	0.30447008917983
1106	32-33	0.15291602900669687
1106	34-35	0.1506905069587745
1106	36-37	0.14861247560425994
1106	38-39	0.17926250774354457
1106	40-41	0.21124950416842125
1106	42-43	0.23812380922661447
1106	44-45	0.30476467975773147
1106	46-47	0.437405098221312
1106	48-49	0.4552389426975907
1106	50-51	0.5922406042829351
1106	52-53	0.6368413349944149
1106	54-55	0.5550176198294778
1106	56-57	0.4606332252011569
1106	58-59	0.6216482061962267
1106	60-61	0.38465274693085405
1106	62-63	0.5364079912931459
1106	64-65	0.49767792784853526
1106	66-67	0.49921970104714575
1106	68-69	0.5011672103978952
1106	70-71	0.5018707096674646
1106	72-73	0.2873740901152644
1106	74-75	0.3675547992535755
1106	76-77	0.40378467798588247
1106	78-79	0.38634422781970557
1106	80-81	0.3939364832067298
1106	82-83	0.4509696594813377
1106	84-85	0.4156525143257248
1106	86-87	0.4349575582019156
1106	88-89	0.5063898191838128
1106	90-91	0.46477091035395546
1106	92-93	0.5168892858152887
1106	94-95	0.5207806184056238
1106	96-97	0.5793177633542932
1106	98-99	0.7419443100352581
1106	100-101	0.7780480060091115
1107	1	0.09724889673237058
1107	2	0.14969009335801786
1107	3	0.2690060900352407
1107	4	0.13303413505963846
1107	5	0.22225861802807145
1107	6	0.24697327976875982
1107	7	0.21102908709999468
1107	8	0.2181645913518011
1107	9	0.2703758933877083
1107	10-11	0.20868042645406604
1107	12-13	0.16724273106489562
1107	14-15	0.37228262946484136
1107	16-17	0.42879820202761465
1107	18-19	0.3424020943314616
1107	20-21	0.2629250967012382
1107	22-23	0.4013959577146764
1107	24-25	0.4060779707614799
1107	26-27	0.2986208181334433
1107	28-29	0.32357286334011093
1107	30-31	0.42488813129911307
1107	32-33	0.457167341206393
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2313	1	-0.007036323857480653
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2314	1	0.11285343780905777
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2314	4	-0.04270320129061389
2314	5	-0.00158093813610094
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2314	10-11	-0.40071120938264926
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2314	90-91	-0.17826715264109794
2314	92-93	-0.23459164594016002
2314	94-95	-0.04745780907731856
2314	96-97	-5.324579000820506E-4
2314	98-99	-0.013379881277213457
2314	100-101	0.04503112314744939
2315	1	-0.396023196431905
2315	2	-0.6471280659543233
2315	3	-0.5910066052832335
2315	4	-1.090523636149804
2315	5	-1.1720406165254431
2315	6	-0.8394961922183697
2315	7	-0.7892045690682821
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2315	9	-1.0390004504829662
2315	10-11	-1.085821477009219
2315	12-13	-0.96113047124296
2315	14-15	-0.8562539652635408
2315	16-17	-0.6781125685759974
2315	18-19	-0.6022547732405599
2315	20-21	-0.8535914458773135
2315	22-23	-0.9990834824896524
2315	24-25	-0.8794971544253727
2315	26-27	-0.728974809825857
2315	28-29	-0.7401743807688277
2315	30-31	-0.5725919981909158
2315	32-33	-0.5234283485048934
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2315	92-93	-0.43356696489387403
2315	94-95	-0.35882649205104755
2315	96-97	-0.30545820324875805
2315	98-99	-0.6411432748009851
2315	100-101	-0.6590562434144438
2316	1	-0.3653077787726424
2316	2	-0.4621070266807834
2316	3	-0.7143270837611304
2316	4	-0.39913968364894004
2316	5	-0.48409446279016066
2316	6	-1.0476755315213566
2316	7	-0.850022516725069
2316	8	-0.5887909010271173
2316	9	-0.5718596023095799
2316	10-11	-0.9979717861640296
2316	12-13	-0.7924837587710059
2316	14-15	-0.9392069033152168
2316	16-17	-0.785656165615741
2316	18-19	-0.7355037916251348
2316	20-21	-0.7052432913692286
2316	22-23	-0.5990962138067033
2316	24-25	-0.6126221539964192
2316	26-27	-0.5418685455374401
2316	28-29	-0.7297954404948328
2316	30-31	-0.7922025846070397
2316	32-33	-0.6797105843026188
2316	34-35	-1.089605343711284
2316	36-37	-0.8418342892375961
2316	38-39	-0.6690186902646929
2316	40-41	-0.6104847867705416
2316	42-43	-0.5839984415588759
2316	44-45	-0.5538979409367997
2316	46-47	-0.5356011415275646
2316	48-49	-0.7680139128930037
2316	50-51	-0.7719844919340559
2316	52-53	-0.762774238759178
2316	54-55	-0.7175539871046297
2316	56-57	-0.7269126011754068
2316	58-59	-0.6643635960281742
2316	60-61	-0.8152599436995089
2316	62-63	-0.6705910399114927
2316	64-65	-0.7151751008884659
2316	66-67	-0.6214851343901628
2316	68-69	-0.7740678476267604
2316	70-71	-0.6321418110556323
2316	72-73	-0.6258959034359179
2316	74-75	-0.5147542317449378
2316	76-77	-0.5311072674671138
2316	78-79	-0.6621264594949636
2316	80-81	-0.5661905202709221
2316	82-83	-0.4536982262773641
2316	84-85	-0.4827594268869504
2316	86-87	-0.38805619044721595
2316	88-89	-0.2588635546681388
2316	90-91	-0.3248488448264517
2316	92-93	-0.4001491450306851
2316	94-95	-0.4936909464197434
2316	96-97	-0.5621035389540303
2316	98-99	-0.4526981632492557
2316	100-101	-0.42985135705946576
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	11450.0
3	1613.0
4	1145.0
5	1152.0
6	1248.0
7	1389.0
8	1634.0
9	1890.0
10	2201.0
11	2552.0
12	3207.0
13	3890.0
14	4556.0
15	5495.0
16	6487.0
17	8480.0
18	8170.0
19	5072.0
20	4707.0
21	4559.0
22	4857.0
23	5233.0
24	5804.0
25	6578.0
26	7890.0
27	9006.0
28	10876.0
29	13360.0
30	16233.0
31	20559.0
32	26072.0
33	34767.0
34	49675.0
35	77262.0
36	136223.0
37	264214.0
38	367224.0
39	121540.0
40	136.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.10794394605986	19.71493922820779	14.672058346014019	23.50505847971833
2	16.504702917894328	21.50265747890492	38.62107168268795	23.37156792051281
3	18.794129256941304	25.333695174670684	30.977783366334112	24.8943922020539
4	13.982428647718978	19.0054414048168	36.807438576273185	30.20469137119104
5	14.52518812309808	36.285714740447425	34.35355364850584	14.835543487948662
6	29.689860743248996	36.270484140579065	18.838049880379064	15.201605235792876
7	25.66277819815992	33.04243419560739	23.196262232523573	18.098525373709116
8	24.44477152392962	33.713099135581956	21.825819566160003	20.016309774328413
9	25.442868087482907	16.976921614317526	22.248060227036774	35.3321500711628
10-11	24.07549487180405	26.501280507764385	28.788614597589646	20.63461002284192
12-13	24.951189045978566	25.085886593098838	28.250483335852778	21.712441025069815
14-15	22.56892290907615	26.957651737033572	26.59259079473469	23.880834559155588
16-17	21.554180315604835	29.118437765617088	27.727837785354332	21.59954413342374
18-19	21.377652549579185	27.705014641273866	29.81957350699395	21.097759302152998
20-21	22.358066301442026	26.94439548345846	29.571816615267576	21.125721599831937
22-23	22.218013853137645	26.981909759658805	29.17032357142005	21.629752815783508
24-25	21.92169674683847	27.16300972237084	29.507540694850576	21.407752835940112
26-27	21.670195236947272	27.244142851800845	29.65176551834976	21.433896392902124
28-29	21.538924849931	27.210695725823165	29.767468135325263	21.482911288920572
30-31	22.040705759555504	27.07053140404082	29.419589298727416	21.469173537676255
32-33	21.812167433779123	27.200292127242413	29.218134005416925	21.76940643356154
34-35	22.069586977754525	27.387056925577223	29.12352872138752	21.41982737528073
36-37	21.941024795334947	27.206159386703067	29.27391598239425	21.578899835567736
38-39	21.80186430207215	27.333709116208798	29.310030021524643	21.554396560194412
40-41	22.090897771533115	27.2896593233089	29.090684149955187	21.528758755202798
42-43	21.949796214190304	27.385463464541747	28.86685861171066	21.797881709557288
44-45	22.082835848507866	27.52493856120426	28.714127879427583	21.678097710860296
46-47	22.084381165400117	27.5147273368893	28.643143508135793	21.757747989574792
48-49	22.000829228968264	27.685384799644115	28.524720493335533	21.78906547805209
50-51	22.07312100035608	27.91193970173125	28.18359223474789	21.83134706316478
52-53	22.17674632484562	27.897321620361602	28.0151063602964	21.910825694496378
54-55	22.158237097106507	27.930183292325044	28.022501656639324	21.88907795392912
56-57	22.034584742006864	27.996327171372943	28.07971374852104	21.889374338099152
58-59	22.020558748287996	27.933197791929494	28.20540768035248	21.840835779430034
60-61	22.070691098872466	27.84893561584712	28.245173326650473	21.835199958629936
62-63	21.89287325339853	27.92617155459166	28.277779147947278	21.90317604406253
64-65	22.052199418857025	27.754968223344157	28.137648841792316	22.055183516006498
66-67	21.92002196084048	27.962555175617105	28.004885491324078	22.112537372218345
68-69	21.910011776688524	27.951373416512826	28.13964128843338	21.99897351836527
70-71	21.99078033764587	27.932839994939844	28.059105132634087	22.0172745347802
72-73	21.916533563007594	27.824346930129035	28.037100614431687	22.222018892431688
74-75	21.992362401313212	28.040312774820503	27.853370196818716	22.11395462704757
76-77	21.968818989487637	27.97685474178213	27.889606475707147	22.16471979302308
78-79	21.881243818346313	28.117721761728532	27.881834233411283	22.11920018651387
80-81	21.967249247951735	28.12937020508309	27.729046986455767	22.1743335605094
82-83	22.056266566719266	28.15715614632745	27.726570650318582	22.060006636634704
84-85	22.060274478351094	28.095321073470064	27.728511820646982	22.115892627531856
86-87	21.953083720071554	28.12764589420879	27.72001903452315	22.1992513511965
88-89	21.94496979321901	28.08458853455142	27.7074115418308	22.26303013039877
90-91	22.001609735117807	27.99594343567445	27.828763332732564	22.173683496475174
92-93	21.92471939084097	28.037677708523006	27.909270766351096	22.128332134284932
94-95	22.06131340633041	27.93001414643228	27.776495917584697	22.23217652965261
96-97	21.963728498724194	27.80423969659062	27.919668906704004	22.312362897981185
98-99	21.838864655570873	27.88054507861221	27.829709473322445	22.45088079249447
100-101	21.851238564366067	27.92541115354601	27.865002115112247	22.358348166975674
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2716.0
1	1557.5
2	409.5
3	398.0
4	328.5
5	303.0
6	415.0
7	743.0
8	1158.0
9	1325.0
10	1132.5
11	890.5
12	859.5
13	937.5
14	1064.5
15	1167.0
16	1241.0
17	1323.0
18	1420.5
19	1615.0
20	1795.0
21	2018.0
22	2413.5
23	2952.5
24	3587.5
25	4460.5
26	5995.5
27	8236.0
28	10973.0
29	14463.5
30	18487.0
31	22451.0
32	26181.0
33	29382.0
34	31858.5
35	33674.5
36	35785.5
37	38061.5
38	39497.0
39	39897.0
40	40490.5
41	41860.0
42	42554.0
43	43445.5
44	45005.5
45	46111.0
46	45961.0
47	45453.0
48	45873.5
49	45674.0
50	44278.0
51	42679.5
52	41075.0
53	39449.5
54	36972.0
55	33548.5
56	30232.5
57	27041.5
58	24318.5
59	21662.5
60	18424.0
61	15173.0
62	12179.5
63	9270.5
64	6740.0
65	4884.5
66	3616.0
67	2807.0
68	2245.5
69	1803.0
70	1388.5
71	1033.5
72	795.0
73	598.5
74	445.0
75	389.5
76	308.0
77	206.5
78	141.5
79	97.5
80	75.5
81	62.0
82	49.5
83	39.0
84	30.0
85	23.5
86	18.0
87	12.5
88	10.0
89	10.0
90	6.5
91	3.5
92	1.5
93	1.0
94	2.5
95	3.0
96	4.5
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15114358958873367
2	0.15511687007213887
3	0.14860069007935434
4	0.15328916104977247
5	0.1416077164285612
6	0.12070826108584987
7	0.12817802839465164
8	0.11848322401514295
9	0.11355635621572051
10-11	0.10231197244768382
12-13	0.12046986425684555
14-15	0.1403759994787056
16-17	0.15082572715006126
18-19	0.14744843873916685
20-21	0.1379920311886625
22-23	0.1311977215620396
24-25	0.11598005731059768
26-27	0.12102612352452229
28-29	0.12456234315475292
30-31	0.11395368426406105
32-33	0.11280143292387354
34-35	0.12980707339284778
36-37	0.13171424802488227
38-39	0.11717204145561926
40-41	0.12003280340367099
42-43	0.11514566840908261
44-45	0.1325089041215633
46-47	0.13068119509919693
48-49	0.14438901276694485
50-51	0.13139638558620986
52-53	0.1153840652380869
54-55	0.1067223137842636
56-57	0.12786016595597924
58-59	0.11693364462661494
60-61	0.11590059170092958
62-63	0.11641711816377227
64-65	0.1386277560660073
66-67	0.12901241729616675
68-69	0.13397901790042324
70-71	0.12134398596319472
72-73	0.12344982461939946
74-75	0.10612632171175279
76-77	0.1309195919282012
78-79	0.13215130887805684
80-81	0.13282676656023573
82-83	0.13866748887084135
84-85	0.1288932188816646
86-87	0.13842909204183704
88-89	0.12468154156925508
90-91	0.13330356021824435
92-93	0.14732924032466468
94-95	0.1233306262048973
96-97	0.10795403073411919
98-99	0.11228490646103086
100-101	0.1566664494606669
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1258406.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.14098459857352
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.84327364862452	34.65239984382746
2	20.317045539038453	22.406037903132724
3	8.376149738950991	13.856074312525266
4	3.715998194982643	8.196151969514597
5	1.8662389698851485	5.145312714784734
6	0.9669022230381096	3.1989564353322537
7	0.5883010022894173	2.270764755459629
8	0.3724569927880821	1.6430116242366912
9	0.22565436497119284	1.119852347712969
>10	0.7134979660593389	5.7754809341915685
>50	0.009712100842490861	0.373720196597759
>100	0.003902126944526126	0.37136136120112173
>500	1.445219308383916E-4	0.0644698698407533
>1k	7.22609654191958E-4	0.9264057316424686
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3774	0.2999032108874243	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3604	0.2863940572438466	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1821	0.14470687520561726	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1333	0.10592765768758254	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5893121933620787E-4
2	0.0	0.0	0.0	0.0	1.5893121933620787E-4
3	0.0	0.0	0.0	0.0	1.5893121933620787E-4
4	0.0	0.0	0.0	0.0	1.5893121933620787E-4
5	0.0	0.0	0.0	0.0	1.5893121933620787E-4
6	0.0	0.0	0.0	0.0	1.5893121933620787E-4
7	0.0	0.0	0.0	0.0	1.5893121933620787E-4
8	0.0	0.0	0.0	0.0	1.5893121933620787E-4
9	0.0	0.0	0.0	0.0	1.5893121933620787E-4
10-11	0.0	0.0	0.0	1.191984145021559E-4	1.5893121933620787E-4
12-13	0.0	0.0	0.0	1.5893121933620787E-4	3.1786243867241575E-4
14-15	0.0	0.0	0.0	1.5893121933620787E-4	3.1786243867241575E-4
16-17	0.0	0.0	0.0	1.9866402417025985E-4	5.165264628426755E-4
18-19	0.0	0.0	0.0	2.383968290043118E-4	5.562592676767275E-4
20-21	0.0	0.0	0.0	2.383968290043118E-4	5.562592676767275E-4
22-23	0.0	0.0	0.0	3.1786243867241575E-4	5.562592676767275E-4
24-25	0.0	0.0	0.0	9.138545111831952E-4	5.562592676767275E-4
26-27	0.0	0.0	0.0	0.0015495793885280267	5.562592676767275E-4
28-29	0.0	0.0	0.0	0.0029004947528857936	5.562592676767275E-4
30-31	0.0	0.0	0.0	0.0061585847492780545	5.562592676767275E-4
32-33	0.0	0.0	0.0	0.013946214496752238	5.562592676767275E-4
34-35	0.0	0.0	0.0	0.03051479411255191	5.562592676767275E-4
36-37	0.0	0.0	0.0	0.05236783677128049	5.562592676767275E-4
38-39	0.0	0.0	0.0	0.07481687150251987	5.959920725107795E-4
40-41	0.0	0.0	0.0	0.09591499086940145	6.357248773448315E-4
42-43	0.0	0.0	0.0	0.12058906267134772	7.151904870129355E-4
44-45	0.0	0.0	0.0	0.1542824811706238	7.946560966810394E-4
46-47	0.0	0.0	0.0	0.19119425686145808	7.946560966810394E-4
48-49	0.0	0.0	0.0	0.2242519504833893	8.741217063491433E-4
50-51	0.0	0.0	0.0	0.2541707525234304	8.741217063491433E-4
52-53	0.0	0.0	0.0	0.2900494752885794	9.535873160172472E-4
54-55	0.0	0.0	0.0	0.33538460560423267	9.535873160172472E-4
56-57	0.0	0.0	0.0	0.3923217149314291	9.535873160172472E-4
58-59	0.0	0.0	0.0	0.4475105808459273	9.535873160172472E-4
60-61	0.0	0.0	0.0	0.49534887786612586	9.535873160172472E-4
62-63	0.0	0.0	0.0	0.548193508295415	0.001033052925685351
64-65	0.0	0.0	0.0	0.6151432844407926	0.001033052925685351
66-67	0.0	0.0	0.0	0.7013237381258512	0.001033052925685351
68-69	0.0	0.0	0.0	0.7883783135172591	0.001072785730519403
70-71	0.0	0.0	0.0	0.8683207168433718	0.001112518535353455
72-73	0.0	0.0	0.0	0.9574016652813162	0.001112518535353455
74-75	0.0	0.0	0.0	1.0636869182124054	0.001112518535353455
76-77	0.0	0.0	0.0	1.1822496078372162	0.001231716949855611
78-79	0.0	0.0	0.0	1.3092753848916805	0.0013509153643577669
80-81	0.0	0.0	0.0	1.4371355508476595	0.0013509153643577669
82-83	0.0	0.0	0.0	1.5744123915493091	0.0015098465836939747
84-85	0.0	0.0	0.0	1.7473692909919376	0.0015495793885280267
86-87	0.0	0.0	0.0	1.9537017464951694	0.0015893121933620788
88-89	0.0	0.0	0.0	2.174059882104822	0.0015893121933620788
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2380	0.0	57.68258	1
GTATCAA	4025	0.0	38.238686	1
GTGGTAT	970	0.0	35.74991	1
TATCAAC	4935	0.0	31.668869	2
TCAACGC	4905	0.0	31.568254	4
ATCAACG	4905	0.0	31.47142	3
CAACGCA	5090	0.0	30.514194	5
AACGCAG	5245	0.0	29.700636	6
GCGTCAG	390	0.0	29.232801	1
TGGTATC	1175	0.0	29.108408	2
CGTCAGA	430	0.0	26.513472	2
ACGCAGA	6130	0.0	25.56867	7
CGCAGAG	6130	0.0	25.260756	8
GAGTACT	4120	0.0	22.946383	12-13
GCAGAGT	6730	0.0	22.444056	9
CAGAGTA	6465	0.0	21.161987	10-11
GTACTTT	4750	0.0	19.802954	14-15
GTACATG	3210	0.0	19.682055	1
AGAGTAC	5905	0.0	19.18673	10-11
TATGCCG	125	0.0060331854	18.999054	5
>>END_MODULE