FastQCFastQC Report
Fri 10 Feb 2017
SRR2031718_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031718_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258406
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51740.4111550644227697No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT51580.4098836146680801No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48780.38763324396101095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22690.18030746833692782No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT19650.1561499229978242RNA PCR Primer, Index 39 (95% over 23bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG18390.14613725617964313RNA PCR Primer, Index 39 (96% over 30bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14890.11832429279580675No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT14580.11586085889609553RNA PCR Primer, Index 39 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29350.059.0948871
GTGGTAT12450.040.8394171
TGGTATC13000.038.0044362
GTATCAA48900.036.4407731
TCAACGC58600.030.562444
ATCAACG58550.030.5074043
TATCAAC60150.029.9328372
CAACGCA60200.029.665345
AACGCAG62200.028.6351136
ACGCAGA69600.025.5905747
CGCAGAG70850.024.8038948
CAGAGTA73250.022.82497610-11
GCAGAGT76100.022.6558289
GAGTACT51000.022.5848312-13
GTACATG35150.021.2245961
GTACTTT55150.020.4547114-15
TACATGG36300.019.8921282
TACGCGA600.004767306619.79004544-45
AGAGTAC69250.018.31330710-11
AGTACTT53200.017.9903112-13
Produced by FastQC (version 0.11.5)