FastQCFastQC Report
Fri 10 Feb 2017
SRR2031717_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031717_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519106
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41730.27470104127032613No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41230.27140963171760235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18930.12461276566612205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26050.055.62481
GTATCAA44300.037.642611
GTGGTAT12000.034.0481531
TGGTATC12300.032.8314552
TCAACGC49350.032.825774
ATCAACG49450.032.7593883
TATCAAC50750.032.203182
CAACGCA50900.031.8251135
AACGCAG52200.031.2052886
ACGCAGA58400.027.4867087
CGCAGAG58650.027.2129758
GCGTCAG3550.022.7508131
GAGTACT47950.022.741212-13
GCAGAGT69850.022.1014989
CAGAGTA65850.022.07709710-11
GTACATG32000.020.042881
GTACTTT53950.019.99254214-15
ACATGGG31550.018.6711043
TACATGG32650.018.6252942
AGAGTAC61500.017.92211310-11
Produced by FastQC (version 0.11.5)