##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031717_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1519106 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.969198989405612 33.0 31.0 34.0 30.0 34.0 2 32.281882238632456 34.0 31.0 34.0 31.0 34.0 3 32.388897812265895 34.0 31.0 34.0 31.0 34.0 4 35.80673501388316 37.0 37.0 37.0 35.0 37.0 5 35.8079916740504 37.0 37.0 37.0 35.0 37.0 6 35.83115661448247 37.0 37.0 37.0 35.0 37.0 7 35.814591608485514 37.0 37.0 37.0 35.0 37.0 8 35.805215040951715 37.0 37.0 37.0 35.0 37.0 9 37.58602098866044 39.0 38.0 39.0 35.0 39.0 10-11 37.515359033536825 39.0 38.0 39.0 35.0 39.0 12-13 37.467509838023155 39.0 38.0 39.0 35.0 39.0 14-15 38.865627875869095 41.0 39.0 41.0 36.0 41.0 16-17 38.790851658804584 41.0 39.0 41.0 36.0 41.0 18-19 38.80613663562649 41.0 39.0 41.0 36.0 41.0 20-21 38.81417425775423 41.0 39.0 41.0 35.5 41.0 22-23 38.715831548292215 41.0 39.0 41.0 35.0 41.0 24-25 38.647283336383374 41.0 39.0 41.0 35.0 41.0 26-27 38.528805758123525 40.5 39.0 41.0 35.0 41.0 28-29 38.41677539289556 40.0 38.0 41.0 34.0 41.0 30-31 38.24813508734742 40.0 38.0 41.0 34.0 41.0 32-33 38.0358269929814 40.0 38.0 41.0 34.0 41.0 34-35 37.86182531041283 40.0 38.0 41.0 33.5 41.0 36-37 37.69065917717394 40.0 38.0 41.0 33.0 41.0 38-39 37.49689389680509 40.0 38.0 41.0 33.0 41.0 40-41 37.39224353007624 40.0 38.0 41.0 33.0 41.0 42-43 37.35276438905514 40.0 38.0 41.0 33.0 41.0 44-45 37.20322018345 40.0 37.5 41.0 32.0 41.0 46-47 37.0995628349832 40.0 37.5 41.0 31.5 41.0 48-49 37.11826330749796 40.0 38.0 41.0 32.0 41.0 50-51 36.56903994849603 39.5 37.0 40.5 30.5 41.0 52-53 36.58869657548584 39.5 37.0 40.5 31.0 41.0 54-55 36.8150714959983 40.0 37.0 41.0 31.0 41.0 56-57 36.69357964487008 40.0 37.0 41.0 31.0 41.0 58-59 36.51018131716944 40.0 36.5 41.0 31.0 41.0 60-61 36.28051630366808 39.5 36.0 41.0 30.0 41.0 62-63 36.00550784474552 39.0 35.0 41.0 29.5 41.0 64-65 35.736935737203325 39.0 35.0 41.0 29.0 41.0 66-67 35.44649846686143 38.5 35.0 41.0 29.0 41.0 68-69 35.1086267186095 37.5 35.0 40.0 28.5 41.0 70-71 34.746259642184285 37.0 35.0 40.0 28.0 41.0 72-73 34.370807237941264 36.5 35.0 39.0 28.0 41.0 74-75 33.95942580702071 36.0 34.5 39.0 27.0 41.0 76-77 33.568710478399794 36.0 34.0 38.5 26.0 40.0 78-79 33.17366727535801 35.0 34.0 37.0 26.0 39.0 80-81 32.81289883655255 35.0 34.0 37.0 26.0 39.0 82-83 32.47908901682963 35.0 34.0 36.5 25.5 38.5 84-85 32.15177150244946 35.0 34.0 36.0 25.0 37.0 86-87 31.876165323552144 35.0 34.0 36.0 24.5 37.0 88-89 31.60887785315837 35.0 33.0 35.0 24.0 36.5 90-91 31.37458314298015 35.0 33.0 35.0 23.0 36.0 92-93 31.16337273369995 35.0 33.0 35.0 20.0 36.0 94-95 30.919714292485185 35.0 33.0 35.0 18.5 36.0 96-97 30.668376992783912 35.0 33.0 35.0 13.5 35.5 98-99 30.428922339849883 35.0 32.0 35.0 2.0 35.0 100-101 29.860901082610432 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0076944281863582376 1101 2 0.0849049691603696 1101 3 0.22276592087771974 1101 4 0.20284569367912297 1101 5 0.1968150126926318 1101 6 0.26945279972752445 1101 7 0.2820440254486769 1101 8 0.21965733458969794 1101 9 0.3532547997241764 1101 10-11 0.2916266116437285 1101 12-13 0.21088990341954883 1101 14-15 0.1892254390731054 1101 16-17 0.3184949963975896 1101 18-19 0.33818830463030736 1101 20-21 0.335678711324384 1101 22-23 0.3643832373475888 1101 24-25 0.19966553439028445 1101 26-27 0.05656567688355807 1101 28-29 0.15791430846010712 1101 30-31 0.15530063434100327 1101 32-33 0.015885978633470188 1101 34-35 0.11173971134534355 1101 36-37 -0.014725002642506979 1101 38-39 0.13567310114061826 1101 40-41 -0.013809769428981156 1101 42-43 -0.0517215576465091 1101 44-45 -0.3001744976556111 1101 46-47 -0.34820473319856404 1101 48-49 -0.08827562221786422 1101 50-51 -0.14515956165505628 1101 52-53 -0.3220237225007594 1101 54-55 -0.036761053569499325 1101 56-57 -0.26204110322880325 1101 58-59 -0.394427346312213 1101 60-61 -0.603985955090522 1101 62-63 -0.3939481267432967 1101 64-65 -0.22243117426021541 1101 66-67 -0.5862822236120309 1101 68-69 -0.6849381393668352 1101 70-71 -0.6395338107492705 1101 72-73 -0.4519791841260954 1101 74-75 -0.3348279096343276 1101 76-77 -0.33974475707533003 1101 78-79 -0.46826650697794037 1101 80-81 -0.5763641363998957 1101 82-83 -0.4790722329222987 1101 84-85 -0.596977607543991 1101 86-87 -0.774751484077175 1101 88-89 -0.8277456593862205 1101 90-91 -1.0452814691843564 1101 92-93 -0.8667274398062048 1101 94-95 -0.7587804782887986 1101 96-97 -1.03054252504716 1101 98-99 -0.7432341475292006 1101 100-101 -0.8997888694579608 1102 1 0.16169811444479976 1102 2 0.26705777970983746 1102 3 0.19633408252698104 1102 4 0.3555618606826556 1102 5 0.2763046350737355 1102 6 0.19374144385518122 1102 7 0.0860300520953956 1102 8 0.015147158794938775 1102 9 0.14421664012284197 1102 10-11 0.16286103334338264 1102 12-13 0.13484108572313147 1102 14-15 0.3284561923223208 1102 16-17 0.3004922836437913 1102 18-19 0.2030982109304773 1102 20-21 0.20459096828212608 1102 22-23 0.24080216488975026 1102 24-25 0.08185870976654286 1102 26-27 0.25062700174028407 1102 28-29 0.22480031047241766 1102 30-31 0.04779337015618523 1102 32-33 0.24161143251517103 1102 34-35 0.3087366869513417 1102 36-37 0.17271916182042446 1102 38-39 0.15021512359651723 1102 40-41 0.22626775478249073 1102 42-43 0.29414178960676196 1102 44-45 0.05304741688373582 1102 46-47 0.19243789858814608 1102 48-49 0.28143057526705206 1102 50-51 0.2086939437036719 1102 52-53 0.1884925020820134 1102 54-55 0.21910683472678016 1102 56-57 0.22158799150091824 1102 58-59 0.08540508029651761 1102 60-61 0.010844557156929113 1102 62-63 0.1376572358426671 1102 64-65 0.158316584156303 1102 66-67 0.1004902820049125 1102 68-69 0.1087518884433436 1102 70-71 0.1028172029717922 1102 72-73 0.04991024592848703 1102 74-75 -0.18775895998054182 1102 76-77 0.02601488818907427 1102 78-79 -0.09677386910682628 1102 80-81 0.10918733320627894 1102 82-83 0.13564846325224522 1102 84-85 0.1835873219511157 1102 86-87 0.10585499744553317 1102 88-89 0.09722384439353249 1102 90-91 0.03258821772111631 1102 92-93 0.08034269704836561 1102 94-95 -0.06604199191044202 1102 96-97 -0.32881930310294294 1102 98-99 -0.06091661287035777 1102 100-101 -0.11756037485823967 1103 1 0.09592819186718415 1103 2 0.23874947745993325 1103 3 0.1929973603351698 1103 4 0.09597246792302627 1103 5 0.18875307965570443 1103 6 0.17090688475246907 1103 7 0.10226823784337569 1103 8 0.24149173656470424 1103 9 0.18849337951962752 1103 10-11 0.170026655584266 1103 12-13 0.18250951708967733 1103 14-15 0.23172808417806579 1103 16-17 0.31296022266246837 1103 18-19 0.2558706145151888 1103 20-21 0.17008213174864295 1103 22-23 0.26960377839440497 1103 24-25 0.21947838237334594 1103 26-27 0.24918135324991653 1103 28-29 0.18830179465642516 1103 30-31 0.15838774646454823 1103 32-33 0.2035785798557086 1103 34-35 0.24221305504710955 1103 36-37 0.04817067904296124 1103 38-39 0.1841626450262268 1103 40-41 0.3208353239735757 1103 42-43 0.384057895525018 1103 44-45 0.22238550141932478 1103 46-47 0.23443990923527736 1103 48-49 0.25564294902073925 1103 50-51 0.420562768812502 1103 52-53 0.26347156256350246 1103 54-55 0.2405566654538731 1103 56-57 0.2116645298329871 1103 58-59 0.25708079714208765 1103 60-61 0.3420539036059438 1103 62-63 0.26304159926430515 1103 64-65 0.32551084735159463 1103 66-67 0.14988431104028876 1103 68-69 0.08258344064253009 1103 70-71 0.021171781840962467 1103 72-73 0.037965369848500075 1103 74-75 0.05472378120927601 1103 76-77 -0.03777697243023681 1103 78-79 -0.017925389616223697 1103 80-81 0.08437527884400708 1103 82-83 0.052697934919002876 1103 84-85 0.09883214147456698 1103 86-87 0.18468619262450758 1103 88-89 0.17576148962425364 1103 90-91 0.21667180351873938 1103 92-93 -0.18211645813981647 1103 94-95 0.19022767961963183 1103 96-97 0.2623197387001568 1103 98-99 0.23818131607242066 1103 100-101 0.16399256103792936 1104 1 -0.2520445756136773 1104 2 -0.12383339750856948 1104 3 -0.05293492332097571 1104 4 -0.12063315584416756 1104 5 -0.1193538953101907 1104 6 -0.04662850275046537 1104 7 -0.10208970381611948 1104 8 -0.24638983586562802 1104 9 -0.10870733648534525 1104 10-11 5.962227662124064E-4 1104 12-13 0.02112382154820125 1104 14-15 0.08478088085524149 1104 16-17 0.11052915117493711 1104 18-19 0.006905736992010247 1104 20-21 0.015136353484258791 1104 22-23 0.08432174282008731 1104 24-25 0.015274677221420063 1104 26-27 0.03949682121650966 1104 28-29 -0.06319549425679583 1104 30-31 0.05586608818313721 1104 32-33 0.0443065761714152 1104 34-35 0.011476325903792883 1104 36-37 0.11627370034062778 1104 38-39 0.06252760770534138 1104 40-41 0.19726635635889522 1104 42-43 0.19954161162556971 1104 44-45 0.15276455392925214 1104 46-47 0.14087102851440392 1104 48-49 0.16377229914195368 1104 50-51 0.1542348793761903 1104 52-53 0.23972565743415686 1104 54-55 0.19560378362075426 1104 56-57 0.06939818728022118 1104 58-59 0.12020675840483364 1104 60-61 0.10155139671961422 1104 62-63 0.14129631542568433 1104 64-65 0.12575556203577776 1104 66-67 0.1949474393911359 1104 68-69 0.16073192912475776 1104 70-71 0.17116515618816663 1104 72-73 0.21411378012630422 1104 74-75 0.2179586855826301 1104 76-77 0.1367763934013837 1104 78-79 0.060262744165484605 1104 80-81 0.05161359814398736 1104 82-83 -0.024995447248649327 1104 84-85 0.057363701305298775 1104 86-87 0.019724816921755206 1104 88-89 0.205871823035789 1104 90-91 0.2990514773193844 1104 92-93 0.16456593630593375 1104 94-95 0.12222078459604191 1104 96-97 0.11068298522657471 1104 98-99 -0.035490729320905245 1104 100-101 -0.030127117802560832 1105 1 0.12544411310481607 1105 2 0.17500673086517082 1105 3 0.14453378971574438 1105 4 0.13006268441655067 1105 5 0.2923488351132235 1105 6 0.28560368663389113 1105 7 0.18462026417315514 1105 8 0.2822306013660665 1105 9 0.34598343334351966 1105 10-11 0.3448660350857935 1105 12-13 0.31216288786084334 1105 14-15 0.36302496466212375 1105 16-17 0.3804686451243313 1105 18-19 0.2438894917584662 1105 20-21 0.4169422295605827 1105 22-23 0.25161239105784006 1105 24-25 0.20720355055987483 1105 26-27 0.27310787029134787 1105 28-29 0.22760731722735983 1105 30-31 0.3771747228059965 1105 32-33 0.4276328787264774 1105 34-35 0.31740099731830185 1105 36-37 0.3876079766254037 1105 38-39 0.30750783881116206 1105 40-41 0.35825722048289776 1105 42-43 0.3046750103037894 1105 44-45 0.26650858810710787 1105 46-47 0.26365581639169733 1105 48-49 0.542187396290764 1105 50-51 0.3929851171819223 1105 52-53 0.5289036353823349 1105 54-55 0.37271891054697903 1105 56-57 0.4233404894051702 1105 58-59 0.483531530731689 1105 60-61 0.5016750557020515 1105 62-63 0.512198302240904 1105 64-65 0.5334626391344628 1105 66-67 0.5305244181416384 1105 68-69 0.4961646735270122 1105 70-71 0.42887988442791425 1105 72-73 0.31522619399927265 1105 74-75 0.530574486636425 1105 76-77 0.5911497442703038 1105 78-79 0.5998698063754659 1105 80-81 0.6142511118953564 1105 82-83 0.6386774519961591 1105 84-85 0.6718821550303673 1105 86-87 0.5968834726369572 1105 88-89 0.667274401263537 1105 90-91 0.3569664793459033 1105 92-93 0.5267295962809513 1105 94-95 0.5870942926235365 1105 96-97 0.6288859803381186 1105 98-99 0.6947176298608788 1105 100-101 0.6970776515465538 1106 1 0.11117179865533089 1106 2 0.18461689488908917 1106 3 0.3170358369129431 1106 4 0.21530251667044809 1106 5 0.22471043217518627 1106 6 0.351880269179361 1106 7 0.2665945067101916 1106 8 0.31650490097647577 1106 9 0.3701815524035439 1106 10-11 0.38569856194749974 1106 12-13 0.36913551745463025 1106 14-15 0.36761713816518693 1106 16-17 0.3596227184264009 1106 18-19 0.2169349767580968 1106 20-21 0.36680302015092536 1106 22-23 0.46000887732301265 1106 24-25 0.39296473570095003 1106 26-27 0.33402898495919686 1106 28-29 0.35535713732214447 1106 30-31 0.3625912343819664 1106 32-33 0.5371720732474401 1106 34-35 0.5358915305821554 1106 36-37 0.4885544020950192 1106 38-39 0.5660656194012503 1106 40-41 0.5425148943705977 1106 42-43 0.47707085139165173 1106 44-45 0.5238025656251537 1106 46-47 0.6414996834851721 1106 48-49 0.6646494921244113 1106 50-51 0.5820272770369996 1106 52-53 0.6254566515862123 1106 54-55 0.629770450287424 1106 56-57 0.7209625147655956 1106 58-59 0.7781892145451863 1106 60-61 0.6067560750821812 1106 62-63 0.8095565493438102 1106 64-65 0.8038695971235015 1106 66-67 0.8372866206776806 1106 68-69 0.9355711104113027 1106 70-71 0.8603610626209175 1106 72-73 0.8907950666760129 1106 74-75 0.8329733054656785 1106 76-77 0.7530629431089793 1106 78-79 0.7566662529374142 1106 80-81 0.8196983934656217 1106 82-83 0.7545805761431623 1106 84-85 0.7907116545216795 1106 86-87 0.6383797876819948 1106 88-89 0.7247753318077237 1106 90-91 0.8294608340445357 1106 92-93 0.8662618427386803 1106 94-95 0.8438435916135845 1106 96-97 0.7820426343493807 1106 98-99 0.874918627404238 1106 100-101 0.8913641102676131 1107 1 0.11045995854066959 1107 2 0.22370068606142013 1107 3 0.33343363928574377 1107 4 0.3532821859647157 1107 5 0.2769035473382857 1107 6 0.3438583990729285 1107 7 0.38776816703347095 1107 8 0.3266288492738454 1107 9 0.3883178159064755 1107 10-11 0.458767543023491 1107 12-13 0.42730442686886505 1107 14-15 0.5023776021087016 1107 16-17 0.48141081195826274 1107 18-19 0.5109942171434767 1107 20-21 0.41252401704558395 1107 22-23 0.45077486187592086 1107 24-25 0.3568878187489659 1107 26-27 0.3440913572287556 1107 28-29 0.3178974138834434 1107 30-31 0.4928324956288819 1107 32-33 0.3990543887184117 1107 34-35 0.45657656389160195 1107 36-37 0.4951188844051799 1107 38-39 0.3039027614242329 1107 40-41 0.3401248420840517 1107 42-43 0.49367275706354974 1107 44-45 0.38360111646519357 1107 46-47 0.39910273043688704 1107 48-49 0.3444992678496561 1107 50-51 0.4000042921921363 1107 52-53 0.38163362958339775 1107 54-55 0.514105455728405 1107 56-57 0.5013388716942231 1107 58-59 0.48954017136234285 1107 60-61 0.5325083908185775 1107 62-63 0.2994238423873057 1107 64-65 0.4464833933386956 1107 66-67 0.382537569532424 1107 68-69 0.5844823825401946 1107 70-71 0.5573916117623057 1107 72-73 0.5386425598415983 1107 74-75 0.5250398181922762 1107 76-77 0.48134226471102437 1107 78-79 0.6098055687003381 1107 80-81 0.6714551681557523 1107 82-83 0.5276267316226324 1107 84-85 0.5608212704677413 1107 86-87 0.5094356042278925 1107 88-89 0.484674209106867 1107 90-91 0.5922498034423072 1107 92-93 0.7605573015066298 1107 94-95 0.7713651564940172 1107 96-97 0.6978697968092398 1107 98-99 0.9202470771215765 1107 100-101 0.8361606705033005 1108 1 -0.15716241716591384 1108 2 -0.17317476736513981 1108 3 -0.16417987843176007 1108 4 -0.03743072488413901 1108 5 -0.0012078847438701246 1108 6 -0.15630851263432533 1108 7 -0.053010138706163445 1108 8 -0.059227387491169736 1108 9 -0.11587019668749576 1108 10-11 -0.10563399479647018 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52-53 -0.4051075874695371 2316 54-55 -0.3514681444642065 2316 56-57 -0.319158909270449 2316 58-59 -0.28552326447090337 2316 60-61 -0.3105125799465611 2316 62-63 -0.371291679309131 2316 64-65 -0.24753799970695667 2316 66-67 -0.11029716277698753 2316 68-69 -0.24963394486446333 2316 70-71 -0.11761178223291324 2316 72-73 -0.04291402012129453 2316 74-75 0.014224599292710138 2316 76-77 -0.015062725549519485 2316 78-79 -0.06027953244821305 2316 80-81 -0.13225752508633093 2316 82-83 -0.02584875698142497 2316 84-85 0.0878112204916377 2316 86-87 -0.051480609468544714 2316 88-89 -0.08349802883732593 2316 90-91 -0.15902997652179707 2316 92-93 -0.15138952415152573 2316 94-95 -0.34337157073452573 2316 96-97 -0.2611764942852943 2316 98-99 -0.324383097408532 2316 100-101 -0.3511038593589788 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11026.0 3 1579.0 4 1129.0 5 1059.0 6 1148.0 7 1402.0 8 1650.0 9 1992.0 10 2104.0 11 2677.0 12 3433.0 13 4260.0 14 5110.0 15 6073.0 16 6868.0 17 9429.0 18 9033.0 19 5553.0 20 4828.0 21 4747.0 22 4999.0 23 5407.0 24 6142.0 25 7046.0 26 8234.0 27 9788.0 28 11693.0 29 14216.0 30 17676.0 31 22409.0 32 29106.0 33 39258.0 34 55979.0 35 88998.0 36 155103.0 37 314241.0 38 480089.0 39 163427.0 40 195.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.89227501031836 20.090142241714652 14.357420054143367 23.660162693823622 2 16.049967988680322 21.93622659398899 39.002644642702904 23.011160774627783 3 18.168865880689946 25.970592462587867 31.357886644769028 24.50265501195316 4 13.490412028282606 19.391544229151336 37.59543796631089 29.52260577625517 5 14.048921534889425 36.913054312871566 34.902411400257634 14.135612751981371 6 29.331965900297785 37.209711586754004 19.041351227723737 14.416971285224472 7 25.127974931019438 33.32621446001369 23.78494674687264 17.76086386209424 8 24.02071375044241 34.468411003306635 22.150557619592778 19.360317626658176 9 25.18959502752563 16.9741510156441 22.254274497087945 35.58197945974232 10-11 23.451057274891472 26.83529247015989 29.56998937370775 20.143660881240887 12-13 24.578820767355094 25.437706404270767 28.75685020780076 21.22662262057338 14-15 22.12002908565435 27.25811013126121 26.94134546861722 23.68051531446721 16-17 21.30000339545253 29.331830982943952 27.954958498010036 21.413207123593484 18-19 21.107471068473753 28.256836397847795 29.90187707966463 20.733815454013822 20-21 21.9843676299915 27.6322394805515 29.60822167864876 20.775171210808242 22-23 21.839488847038414 27.706370493612237 29.131743967368056 21.3223966919813 24-25 21.613461528321885 27.769707738625637 29.48311179538013 21.13371893767235 26-27 21.41589537322803 27.954047004272663 29.582504642590557 21.047552979908744 28-29 21.39041433812055 27.93085367409066 29.6732219146606 21.005510073128185 30-31 21.7557446861797 27.741501561827032 29.456825591093615 21.045928160899656 32-33 21.51613960914317 27.89556500577503 29.220807252320313 21.367488132761487 34-35 21.757620181707082 27.99222441872652 29.203187487352157 21.04696791221424 36-37 21.69088674456247 27.89740324246149 29.34582883028082 21.065881182695225 38-39 21.50793794643225 27.988597698119115 29.36821031644683 21.135254039001804 40-41 21.813626785604825 27.898310245893263 29.183372297576526 21.10469067092538 42-43 21.633009365123147 28.003593275723603 28.94086352637304 21.422533832780204 44-45 21.771545360970514 28.196500428967553 28.72665356857169 21.305300641490245 46-47 21.726955784611064 28.2016227536187 28.680215760709427 21.39120570106081 48-49 21.685536075589273 28.255261469816308 28.719980960279635 21.339221494314785 50-51 21.76672568518389 28.438806410913898 28.425458030715117 21.369009873187093 52-53 21.890190674007457 28.454950996045213 28.18917856381389 21.46567976613344 54-55 21.819613683842864 28.446431748835188 28.26539959536315 21.4685549719588 56-57 21.80731119063423 28.596255089483286 28.154095414999286 21.4423383048832 58-59 21.624692580171367 28.70822542264356 28.285062831785208 21.38201916539986 60-61 21.762116482740076 28.473998070220762 28.427994453202853 21.33589099383631 62-63 21.627589006868646 28.51795329267003 28.435534795863454 21.41892290459787 64-65 21.78697882366281 28.451430488836326 28.273571142749592 21.48801954475127 66-67 21.692662019978037 28.612169806719024 28.176888810374763 21.518279362928176 68-69 21.55371454092101 28.64993053621428 28.249298358083756 21.547056564780952 70-71 21.644697793841157 28.532884431656136 28.26271293965895 21.55970483484375 72-73 21.624795218181823 28.570454008417684 28.2087593064294 21.595991466971096 74-75 21.68722576699569 28.622281065022463 28.057010150163368 21.633483017818474 76-77 21.614181727693556 28.609661359670014 28.102516607367395 21.67364030526904 78-79 21.569618817970518 28.6430681771739 28.20451042769433 21.582802577161246 80-81 21.514861572039592 28.76827641624201 28.080940451208015 21.635921560510383 82-83 21.582192196230753 28.77920084804088 28.072961577102895 21.56564537862547 84-85 21.7102333793318 28.649520344846792 27.969200032035236 21.67104624378617 86-87 21.550003609274153 28.789317603270565 27.9929699909455 21.667708796509782 88-89 21.65664564471138 28.61023739341816 28.02891485580802 21.704202106062443 90-91 21.5259292951266 28.66968576344256 28.136729487610495 21.667655453820345 92-93 21.569662280946098 28.652238939304585 28.150089152766867 21.62800962698245 94-95 21.556462525846346 28.62671119785333 28.14626741518081 21.670558861119517 96-97 21.517194202004568 28.500780783274397 28.187610737277552 21.794414277443487 98-99 21.42189843271923 28.5997682081342 28.146729457635374 21.831603901511194 100-101 21.443072611884944 28.61145635869117 28.130355229539273 21.815115799884612 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2935.0 1 1672.0 2 391.0 3 336.5 4 272.0 5 273.5 6 372.5 7 638.5 8 1054.5 9 1297.5 10 1150.5 11 982.0 12 1012.0 13 1068.0 14 1141.5 15 1245.0 16 1367.0 17 1495.0 18 1648.0 19 1804.5 20 2067.0 21 2425.0 22 2836.5 23 3525.0 24 4417.5 25 5750.5 26 7944.5 27 10961.0 28 14598.0 29 19047.0 30 23790.0 31 28431.0 32 32940.5 33 37389.0 34 41312.5 35 43762.0 36 46158.0 37 48948.0 38 50511.0 39 51349.0 40 52895.5 41 54945.5 42 56561.0 43 57666.5 44 58958.0 45 59978.0 46 60106.5 47 59382.0 48 58398.0 49 56844.0 50 53696.0 51 50422.0 52 47710.5 53 44368.5 54 40405.0 55 35932.5 56 31344.0 57 26824.0 58 23235.0 59 20038.5 60 16203.0 61 13014.5 62 10218.0 63 7654.5 64 5659.5 65 4143.5 66 3100.0 67 2368.5 68 1872.0 69 1498.5 70 1184.0 71 897.5 72 644.0 73 471.5 74 375.5 75 293.5 76 230.0 77 170.0 78 117.0 79 96.5 80 74.5 81 53.5 82 39.5 83 32.0 84 30.5 85 25.5 86 17.0 87 8.5 88 5.5 89 5.5 90 5.5 91 4.0 92 5.5 93 5.5 94 4.5 95 3.5 96 2.0 97 2.5 98 3.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15746103300230532 2 0.16160820903873724 3 0.15482790536012628 4 0.15890925320550378 5 0.14666520966937133 6 0.1256001885319392 7 0.13244632040160464 8 0.12197963802394302 9 0.1180299465606745 10-11 0.10818863199803042 12-13 0.12556727443641194 14-15 0.14528281765722734 16-17 0.15611155508568855 18-19 0.15370882611220021 20-21 0.14413082431377403 22-23 0.13593521452749183 24-25 0.12072890239390799 26-27 0.12530396167219404 28-29 0.13004359142811628 30-31 0.11931359628623676 32-33 0.11839200161147412 34-35 0.1348819634706202 36-37 0.1368897232977817 38-39 0.1229999749852874 40-41 0.1252710475766668 42-43 0.1203010191520539 44-45 0.13767966159043543 46-47 0.13527693261694707 48-49 0.14989079103104064 50-51 0.13613269910065526 52-53 0.12049850372521734 54-55 0.11164461202839038 56-57 0.13211717944633225 58-59 0.12115678563576208 60-61 0.12072890239390799 62-63 0.12174923935525236 64-65 0.14330797192559308 66-67 0.13428950975112994 68-69 0.14011530465945102 70-71 0.1264888691111746 72-73 0.1278712611233186 74-75 0.11138129926417248 76-77 0.13715303606199963 78-79 0.13744926292174475 80-81 0.13754800520832647 82-83 0.14469036393773707 84-85 0.13340082917189452 86-87 0.14353837059428376 88-89 0.12915491084888087 90-91 0.13797588845018055 92-93 0.15321511467929164 94-95 0.12948405180415323 96-97 0.11319157451817055 98-99 0.11826034522936517 100-101 0.16331974200615362 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1519106.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.29400657093859 #Duplication Level Percentage of deduplicated Percentage of total 1 64.74247637894594 37.74098343453111 2 20.45146204119439 23.843953252293744 3 7.944396010270643 13.893320196745657 4 3.2212008650481385 7.5110681757371704 5 1.5112425363907345 4.40481911733217 6 0.7884659241069008 2.7577702656509304 7 0.4140037609469662 1.689375657272502 8 0.25896009078376897 1.2076656987007908 9 0.17273129728290515 0.9062279440934777 >10 0.4765923330119789 4.241693626904543 >50 0.013601562629586773 0.5283724413850973 >100 0.004188135938872125 0.4005741702446517 >500 1.1317724152594061E-4 0.046908615063088535 >1k 5.65886207629703E-4 0.8272674040451015 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4173 0.27470104127032613 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4123 0.27140963171760235 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1893 0.12461276566612205 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.582819105447546E-5 0.0 10-11 0.0 0.0 0.0 6.582819105447546E-5 0.0 12-13 0.0 0.0 0.0 6.582819105447546E-5 0.0 14-15 0.0 0.0 0.0 6.582819105447546E-5 0.0 16-17 0.0 0.0 0.0 9.874228658171319E-5 0.0 18-19 0.0 0.0 0.0 1.9748457316342638E-4 0.0 20-21 0.0 0.0 0.0 2.303986686906641E-4 0.0 22-23 0.0 0.0 0.0 3.291409552723773E-4 0.0 24-25 6.582819105447546E-5 0.0 0.0 5.595396239630415E-4 0.0 26-27 6.582819105447546E-5 0.0 0.0 0.0010861651523988451 0.0 28-29 6.582819105447546E-5 0.0 0.0 0.0021065021137432146 3.291409552723773E-5 30-31 6.582819105447546E-5 0.0 0.0 0.005430825761994226 6.582819105447546E-5 32-33 6.582819105447546E-5 0.0 0.0 0.012704840873513763 6.582819105447546E-5 34-35 6.582819105447546E-5 0.0 0.0 0.02524511126939134 6.582819105447546E-5 36-37 6.582819105447546E-5 0.0 0.0 0.043446606095953806 6.582819105447546E-5 38-39 6.582819105447546E-5 0.0 0.0 0.05911371556691897 6.582819105447546E-5 40-41 6.582819105447546E-5 0.0 0.0 0.07474791094235689 6.582819105447546E-5 42-43 6.582819105447546E-5 0.0 0.0 0.09528630655135323 6.582819105447546E-5 44-45 6.582819105447546E-5 0.0 0.0 0.12411905423321348 6.582819105447546E-5 46-47 6.582819105447546E-5 0.0 0.0 0.15614446918121577 6.582819105447546E-5 48-49 6.582819105447546E-5 0.0 0.0 0.1846809900033309 6.582819105447546E-5 50-51 6.582819105447546E-5 0.0 0.0 0.21315168263439155 6.582819105447546E-5 52-53 6.582819105447546E-5 0.0 0.0 0.2439592760478861 6.582819105447546E-5 54-55 6.582819105447546E-5 0.0 0.0 0.2822712832415908 6.582819105447546E-5 56-57 6.582819105447546E-5 0.0 0.0 0.33147785605481117 6.582819105447546E-5 58-59 6.582819105447546E-5 0.0 0.0 0.3764055964494907 6.582819105447546E-5 60-61 6.582819105447546E-5 0.0 0.0 0.4188318655841001 6.582819105447546E-5 62-63 6.582819105447546E-5 0.0 0.0 0.4669193591493944 6.582819105447546E-5 64-65 6.582819105447546E-5 0.0 0.0 0.5223137819217356 6.582819105447546E-5 66-67 6.582819105447546E-5 0.0 0.0 0.5881090588806838 6.582819105447546E-5 68-69 6.582819105447546E-5 0.0 0.0 0.6631531966827857 6.582819105447546E-5 70-71 6.582819105447546E-5 0.0 0.0 0.7346755262634734 6.582819105447546E-5 72-73 6.582819105447546E-5 0.0 0.0 0.8122540494211727 6.582819105447546E-5 74-75 6.582819105447546E-5 0.0 0.0 0.905335111572201 6.582819105447546E-5 76-77 6.582819105447546E-5 0.0 0.0 1.0123388361312509 6.582819105447546E-5 78-79 6.582819105447546E-5 0.0 0.0 1.1209553513711352 6.582819105447546E-5 80-81 6.582819105447546E-5 0.0 0.0 1.2319416814889812 6.582819105447546E-5 82-83 6.582819105447546E-5 0.0 0.0 1.3567519317282666 6.582819105447546E-5 84-85 6.582819105447546E-5 0.0 0.0 1.520894526122601 6.582819105447546E-5 86-87 6.582819105447546E-5 0.0 0.0 1.7061021416543678 6.582819105447546E-5 88-89 6.582819105447546E-5 0.0 0.0 1.9016776972772145 6.582819105447546E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2605 0.0 55.6248 1 GTATCAA 4430 0.0 37.64261 1 GTGGTAT 1200 0.0 34.048153 1 TGGTATC 1230 0.0 32.831455 2 TCAACGC 4935 0.0 32.82577 4 ATCAACG 4945 0.0 32.759388 3 TATCAAC 5075 0.0 32.20318 2 CAACGCA 5090 0.0 31.825113 5 AACGCAG 5220 0.0 31.205288 6 ACGCAGA 5840 0.0 27.486708 7 CGCAGAG 5865 0.0 27.212975 8 GCGTCAG 355 0.0 22.750813 1 GAGTACT 4795 0.0 22.7412 12-13 GCAGAGT 6985 0.0 22.101498 9 CAGAGTA 6585 0.0 22.077097 10-11 GTACATG 3200 0.0 20.04288 1 GTACTTT 5395 0.0 19.992542 14-15 ACATGGG 3155 0.0 18.671104 3 TACATGG 3265 0.0 18.625294 2 AGAGTAC 6150 0.0 17.922113 10-11 >>END_MODULE