FastQCFastQC Report
Fri 10 Feb 2017
SRR2031717_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031717_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519106
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60290.3968781638674325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59510.3917435649651834No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT44570.2933962475297971No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28030.18451641952569472No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT18160.11954399495492744RNA PCR Primer, Index 39 (95% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15430.10157289879705562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28350.057.9921341
GTATCAA50250.039.9045561
TCAACGC60500.033.140594
ATCAACG60850.032.949973
TATCAAC61100.032.8929142
CAACGCA61950.032.506525
GTGGTAT12900.032.0461351
AACGCAG64900.031.2484936
TGGTATC12100.031.0202242
CGCAGAG70050.028.951148
ACGCAGA70850.028.6912737
GCAGAGT81050.024.9633279
TAAGACG1151.2798248E-424.7888624
GAGTACT61750.023.07438912-13
CAGAGTA78500.022.0533110-11
AGAGTAC74500.020.62357310-11
GTACTTT69900.020.18018714-15
GTACATG29550.019.2961141
AGTACTT68300.018.56677412-13
TACATGG30400.017.8169942
Produced by FastQC (version 0.11.5)