##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031717_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1519106 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.7318429392024 34.0 31.0 34.0 31.0 34.0 2 32.89945731239295 34.0 31.0 34.0 31.0 34.0 3 32.94955190750349 34.0 31.0 34.0 31.0 34.0 4 36.35576319229863 37.0 37.0 37.0 35.0 37.0 5 36.32640711049788 37.0 37.0 37.0 35.0 37.0 6 36.35361324357879 37.0 37.0 37.0 35.0 37.0 7 36.30239561952885 37.0 37.0 37.0 35.0 37.0 8 36.29359504866678 37.0 37.0 37.0 35.0 37.0 9 38.139208850468634 39.0 39.0 39.0 37.0 39.0 10-11 38.10699319204848 39.0 39.0 39.0 36.0 39.0 12-13 38.05679030956365 39.0 39.0 39.0 35.0 39.0 14-15 39.525896810360834 41.0 39.0 41.0 37.0 41.0 16-17 39.50039200687773 41.0 39.0 41.0 36.5 41.0 18-19 39.52331733269436 41.0 39.5 41.0 37.0 41.0 20-21 39.52795394133128 41.0 39.5 41.0 37.0 41.0 22-23 39.439882733660454 41.0 39.0 41.0 36.0 41.0 24-25 39.371115972157305 41.0 39.0 41.0 36.0 41.0 26-27 39.23006228663438 41.0 39.0 41.0 36.0 41.0 28-29 39.124436016973135 40.5 39.0 41.0 36.0 41.0 30-31 38.93363827145703 40.0 38.5 41.0 35.0 41.0 32-33 38.70174530282942 40.0 38.0 41.0 35.0 41.0 34-35 38.544478792131684 40.0 38.0 41.0 35.0 41.0 36-37 38.42357379932671 40.0 38.0 41.0 34.5 41.0 38-39 38.4788243875016 40.0 38.0 41.0 35.0 41.0 40-41 38.38656979828926 40.0 38.0 41.0 35.0 41.0 42-43 38.274497632159964 40.0 38.0 41.0 34.0 41.0 44-45 38.06919793615455 40.0 38.0 41.0 34.0 41.0 46-47 37.814768686319454 40.0 38.0 41.0 33.0 41.0 48-49 37.54072724352349 40.0 38.0 41.0 33.0 41.0 50-51 37.374893193760016 40.0 38.0 41.0 33.0 41.0 52-53 37.43588893730918 40.0 38.0 41.0 33.0 41.0 54-55 37.37394526780883 40.0 38.0 41.0 33.0 41.0 56-57 37.193652055880236 40.0 37.0 41.0 32.5 41.0 58-59 37.01955854298515 40.0 37.0 41.0 32.0 41.0 60-61 36.76577967567766 40.0 36.5 41.0 31.5 41.0 62-63 36.49806267633727 39.5 36.0 41.0 31.0 41.0 64-65 36.18759487488036 39.0 35.5 41.0 31.0 41.0 66-67 35.83246264579299 39.0 35.0 41.0 30.5 41.0 68-69 35.444016414917726 38.0 35.0 40.0 29.5 41.0 70-71 35.091981073078514 37.0 35.0 40.0 29.0 41.0 72-73 34.69763235745235 37.0 35.0 39.0 29.0 41.0 74-75 34.30543227398219 36.0 35.0 39.0 29.0 41.0 76-77 33.35967371598822 35.0 33.5 38.0 27.0 39.0 78-79 33.53068811524673 35.0 34.0 37.0 28.0 39.0 80-81 33.27595803057851 35.0 34.0 37.0 28.0 39.0 82-83 32.97396495043795 35.0 34.0 36.5 28.0 39.0 84-85 32.65480223236562 35.0 34.0 36.0 27.0 37.0 86-87 32.38561858092852 35.0 34.0 36.0 27.0 37.0 88-89 32.13443630661718 35.0 34.0 35.5 26.0 36.5 90-91 31.918781177876987 35.0 34.0 35.0 26.0 36.0 92-93 31.7319584676777 35.0 34.0 35.0 25.5 36.0 94-95 31.547884084454935 35.0 33.0 35.0 25.0 36.0 96-97 31.37189340309366 35.0 33.0 35.0 24.5 36.0 98-99 31.163391165593445 35.0 33.0 35.0 23.5 35.0 100-101 30.467513129432703 34.5 32.0 35.0 17.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21123036145881002 1101 2 0.14011497870094303 1101 3 0.16370007946431286 1101 4 0.11706233551642953 1101 5 0.10920519316702126 1101 6 0.09124190911740726 1101 7 0.16040743101758181 1101 8 0.108222647033962 1101 9 0.051149210149368685 1101 10-11 0.12377421233654928 1101 12-13 0.0672904669088581 1101 14-15 -0.019809712993847484 1101 16-17 0.014848384954177618 1101 18-19 0.07740024684949987 1101 20-21 0.02723736433463131 1101 22-23 -0.03602657458544911 1101 24-25 -0.03449757319234692 1101 26-27 0.054696727544218504 1101 28-29 0.05093574244036603 1101 30-31 0.12434058873841991 1101 32-33 0.17979870417654809 1101 34-35 -0.1669042409372139 1101 36-37 -0.011255593922463447 1101 38-39 0.03103060322129636 1101 40-41 -0.23410475253347585 1101 42-43 -0.280182763725044 1101 44-45 -0.3040145795107563 1101 46-47 -0.32005447637318696 1101 48-49 -0.5074981440712989 1101 50-51 -0.32364997178253674 1101 52-53 -0.3288701574279784 1101 54-55 -0.3109585751721653 1101 56-57 -0.4404845986308814 1101 58-59 -0.26832150538646715 1101 60-61 -0.34231411740278617 1101 62-63 -0.42487543927096283 1101 64-65 -0.25922860204050124 1101 66-67 -0.5821297121089586 1101 68-69 -0.6004014565953284 1101 70-71 -0.5413529255097345 1101 72-73 -0.4740401612556724 1101 74-75 -0.5220385248647688 1101 76-77 -0.4867197227383997 1101 78-79 -0.4781294036952488 1101 80-81 -0.3889096427802201 1101 82-83 -0.3139355388649392 1101 84-85 -0.26416078933701925 1101 86-87 -0.3625291013749177 1101 88-89 -0.43556322892309396 1101 90-91 -0.4312355212914447 1101 92-93 -0.49247175573495383 1101 94-95 -0.5369566611643819 1101 96-97 -0.3944369493542865 1101 98-99 -0.4795662534517575 1101 100-101 -0.7331377831597585 1102 1 0.10806380015621642 1102 2 0.11289958782992215 1102 3 0.12907061967035105 1102 4 0.11274033104662351 1102 5 0.17047543865046322 1102 6 0.1316085167208314 1102 7 0.13706231471642383 1102 8 0.16167339452525908 1102 9 0.1171575739789219 1102 10-11 0.24928567327616946 1102 12-13 0.19096354742777777 1102 14-15 0.21971635645147103 1102 16-17 0.1866372294219616 1102 18-19 0.21017707981332023 1102 20-21 0.22502887406820093 1102 22-23 0.15214237595873215 1102 24-25 0.14235112103518333 1102 26-27 0.19590446690615693 1102 28-29 0.22827860899602825 1102 30-31 0.12273104126967382 1102 32-33 0.20979956489712492 1102 34-35 0.09370206250537194 1102 36-37 0.16930428996929692 1102 38-39 0.05990688799514743 1102 40-41 0.1990119166559836 1102 42-43 0.14877425070241657 1102 44-45 0.1569244369527425 1102 46-47 0.1860311148676459 1102 48-49 0.17599353216717617 1102 50-51 0.0825525587359408 1102 52-53 0.22876873764425198 1102 54-55 0.30323500385236457 1102 56-57 0.2317975277644564 1102 58-59 0.23468597101049937 1102 60-61 0.2602264922841755 1102 62-63 0.28297759714345005 1102 64-65 0.299236287980186 1102 66-67 0.0921982580448173 1102 68-69 0.021762098121264728 1102 70-71 0.07675158971166951 1102 72-73 0.19610470692052218 1102 74-75 0.09932159171835053 1102 76-77 0.22728776256256822 1102 78-79 0.1363033962881275 1102 80-81 0.036806907095574104 1102 82-83 0.14447948872263794 1102 84-85 0.09963967782648808 1102 86-87 0.20842662493968334 1102 88-89 0.2624473193559069 1102 90-91 0.10483757466108656 1102 92-93 0.18317786569639694 1102 94-95 0.21874531582173162 1102 96-97 0.0461546327985225 1102 98-99 -0.14379737770122603 1102 100-101 0.03611401322343255 1103 1 0.21104939580759918 1103 2 0.12010006204342716 1103 3 0.21275275607927568 1103 4 0.15887754241278174 1103 5 0.0947990553671687 1103 6 0.11624928072140506 1103 7 0.11391833810068164 1103 8 0.10403673229017585 1103 9 0.10477690656647809 1103 10-11 0.17195906325670052 1103 12-13 0.11107432569783526 1103 14-15 0.20990623453189983 1103 16-17 0.23437706482998522 1103 18-19 0.2865040332522142 1103 20-21 0.26300959044341 1103 22-23 0.2555512955702497 1103 24-25 0.13850125313265949 1103 26-27 0.25666335585927413 1103 28-29 0.2809416397235722 1103 30-31 0.1350213202328021 1103 32-33 -0.04917892661883627 1103 34-35 0.05750559711876235 1103 36-37 0.07741323893581153 1103 38-39 -0.1251225180025628 1103 40-41 -0.007499621897089526 1103 42-43 -0.04540960677059047 1103 44-45 -0.07079495196466468 1103 46-47 -0.04434665784762615 1103 48-49 0.018833803593203413 1103 50-51 -0.0883538645676154 1103 52-53 -0.1971711038841164 1103 54-55 -0.0320217050106919 1103 56-57 -0.16256500982184008 1103 58-59 -0.1143321667494277 1103 60-61 -0.21216281161709105 1103 62-63 -0.33724499078835635 1103 64-65 -0.1533451947984048 1103 66-67 -0.07766685632066128 1103 68-69 -0.10010023261998668 1103 70-71 -0.14096613311144068 1103 72-73 0.011634065882240918 1103 74-75 -0.04224683772142157 1103 76-77 -0.04032891787487358 1103 78-79 -0.011053010751176373 1103 80-81 -0.08618995452679457 1103 82-83 -0.026248141127879876 1103 84-85 -0.2511168083239568 1103 86-87 -0.1905122883361443 1103 88-89 0.02404934269709713 1103 90-91 -0.08774479216754116 1103 92-93 -0.12430465448091255 1103 94-95 -0.18702401738919505 1103 96-97 -0.17185295643102805 1103 98-99 -0.06323945145593513 1103 100-101 -0.09602681594749995 1104 1 0.05946858022232249 1104 2 0.04701476708240193 1104 3 0.0439256921341169 1104 4 0.022446662091624603 1104 5 0.058569768678431444 1104 6 0.02065631738190632 1104 7 0.11783399028215058 1104 8 0.1053868448176587 1104 9 -0.005458749438318478 1104 10-11 0.06741115001148046 1104 12-13 0.09299443987629985 1104 14-15 0.16064885073064517 1104 16-17 0.15731800745400193 1104 18-19 0.10850532796994372 1104 20-21 0.16940975380376955 1104 22-23 0.190869174839996 1104 24-25 0.12390146428461435 1104 26-27 0.1586873298606477 1104 28-29 0.15176259068939402 1104 30-31 0.1961006064870645 1104 32-33 0.32259514079338203 1104 34-35 0.22591665696252505 1104 36-37 0.2730101124966353 1104 38-39 0.18223717022245722 1104 40-41 0.21073960544317316 1104 42-43 0.1927451380657459 1104 44-45 0.0910078338507887 1104 46-47 -0.010304379665790009 1104 48-49 -0.009106900072843871 1104 50-51 0.0905458208768053 1104 52-53 0.13855452457534767 1104 54-55 0.1277010602978237 1104 56-57 0.08445453259537317 1104 58-59 0.060534559873957505 1104 60-61 0.2914367643411353 1104 62-63 0.3257884285698438 1104 64-65 0.2660396187709253 1104 66-67 0.19213787157138285 1104 68-69 0.3813794385738518 1104 70-71 0.36989613585964776 1104 72-73 0.24697236519665466 1104 74-75 0.3629721129489667 1104 76-77 0.47679513606879453 1104 78-79 0.501466757589931 1104 80-81 0.586784659447467 1104 82-83 0.5219426211914922 1104 84-85 0.6066000134481513 1104 86-87 0.5123136068873038 1104 88-89 0.5170239783948105 1104 90-91 0.4779306333948803 1104 92-93 0.4479089869778541 1104 94-95 0.583241247680359 1104 96-97 0.49209284790149965 1104 98-99 0.47764299860185133 1104 100-101 0.6880018850769822 1105 1 0.176374913712813 1105 2 0.11724914014376253 1105 3 0.08175851676996615 1105 4 0.10232147288097337 1105 5 0.0894565979628652 1105 6 0.0927042176606534 1105 7 0.15224448020332204 1105 8 0.11231733109936926 1105 9 0.11260249277613354 1105 10-11 0.19447850900301944 1105 12-13 0.1757244703981442 1105 14-15 0.21048104259644163 1105 16-17 0.2200289308019734 1105 18-19 0.1979688795882666 1105 20-21 0.20411698392528166 1105 22-23 0.15695962787025763 1105 24-25 0.25377286307863045 1105 26-27 0.194681812767584 1105 28-29 0.25970106310806074 1105 30-31 0.2658185275272942 1105 32-33 0.20779390503071227 1105 34-35 0.24589027086527437 1105 36-37 0.3402460654152364 1105 38-39 0.3176840795750451 1105 40-41 0.22859674830031906 1105 42-43 0.26354972792323395 1105 44-45 0.43060476346856547 1105 46-47 0.3036586425762806 1105 48-49 0.3071147596871171 1105 50-51 0.3162033020384527 1105 52-53 0.2920452025905149 1105 54-55 0.3846894471064317 1105 56-57 0.29579765938925817 1105 58-59 0.3515544983339751 1105 60-61 0.4204991066655879 1105 62-63 0.35418553519780005 1105 64-65 0.3912045318787847 1105 66-67 0.4656118825063089 1105 68-69 0.5029653753630186 1105 70-71 0.4842894830891069 1105 72-73 0.38739303320330265 1105 74-75 0.4035898495346544 1105 76-77 0.3019929284044238 1105 78-79 0.4102739446996253 1105 80-81 0.3154684770773528 1105 82-83 0.3070711810399587 1105 84-85 0.38798726871502964 1105 86-87 0.2907039929183881 1105 88-89 0.3905940421681109 1105 90-91 0.4022060496583073 1105 92-93 0.36230977856077473 1105 94-95 0.27292699257064257 1105 96-97 0.3383018109030651 1105 98-99 0.2907225208397861 1105 100-101 0.23907945524001306 1106 1 0.11441010069015789 1106 2 0.07692704778415305 1106 3 0.06103095529201141 1106 4 0.06920104805653438 1106 5 0.015967429497145247 1106 6 0.0013873115339535502 1106 7 0.0383895458377026 1106 8 0.08307690329719009 1106 9 0.004541250561686638 1106 10-11 -0.04482569209378795 1106 12-13 0.04391549250787108 1106 14-15 0.08876288581836178 1106 16-17 0.049291691664528514 1106 18-19 0.106546263642457 1106 20-21 0.14350332105523478 1106 22-23 0.10053291753005311 1106 24-25 0.20201549937232954 1106 26-27 0.16304405500684283 1106 28-29 0.1768064503385176 1106 30-31 0.060091834557240986 1106 32-33 0.16987584254776777 1106 34-35 0.17422075052978414 1106 36-37 0.06315631132704169 1106 38-39 -0.028025987672279484 1106 40-41 0.02995013175896588 1106 42-43 0.1198650211066905 1106 44-45 -0.0320915813538889 1106 46-47 -0.03163478902251882 1106 48-49 -0.07413613983893441 1106 50-51 -0.06420564111149929 1106 52-53 -0.06409167425506723 1106 54-55 -0.0516849046144543 1106 56-57 0.05240774896963529 1106 58-59 0.06312227917220525 1106 60-61 0.052781793580898295 1106 62-63 -0.018334378447697475 1106 64-65 0.05549868309841344 1106 66-67 0.056713894963195344 1106 68-69 0.2406484444218009 1106 70-71 0.05467683761403208 1106 72-73 0.08534955817910372 1106 74-75 0.05746041704253457 1106 76-77 0.17862262144891616 1106 78-79 0.053557400864789884 1106 80-81 -0.03635861540633556 1106 82-83 -0.03359831448102568 1106 84-85 0.03791580292183738 1106 86-87 0.10206506887560352 1106 88-89 0.0045678380439326816 1106 90-91 0.0717025632194428 1106 92-93 0.044444074697157276 1106 94-95 0.1333143470955669 1106 96-97 0.194987584743604 1106 98-99 0.20691200444980495 1106 100-101 0.29721241139277055 1107 1 0.1583297333573057 1107 2 0.1057807250939149 1107 3 0.09347515201680778 1107 4 0.06863200062706909 1107 5 0.048226367310661544 1107 6 0.03360962222748043 1107 7 0.07724667352833592 1107 8 0.03821611289455973 1107 9 0.05964064807210434 1107 10-11 0.12401232579511401 1107 12-13 0.09111829527879678 1107 14-15 0.22875571964271302 1107 16-17 0.1602126779426314 1107 18-19 0.18490361693491764 1107 20-21 0.24234770887804302 1107 22-23 0.1923427750027784 1107 24-25 0.09883477903897386 1107 26-27 0.15498369988303295 1107 28-29 0.1626362669375041 1107 30-31 0.2168410449508542 1107 32-33 0.20862308887842573 1107 34-35 0.1739176478411366 1107 36-37 0.2423205229150156 1107 38-39 0.2166381310208223 1107 40-41 0.18521010468673893 1107 42-43 0.21610543302908525 1107 44-45 0.2406371390856421 1107 46-47 0.203807788677409 1107 48-49 0.29411743661354706 1107 50-51 0.42558388565549166 1107 52-53 0.36280882206894915 1107 54-55 0.3819510005796971 1107 56-57 0.39433412315017335 1107 58-59 0.41227268923670124 1107 60-61 0.37729595341315303 1107 62-63 0.5102411450812454 1107 64-65 0.3995621701063996 1107 66-67 0.3916898750040687 1107 68-69 0.46956210527590514 1107 70-71 0.4888430398063477 1107 72-73 0.3384588821698742 1107 74-75 0.38836282434672853 1107 76-77 0.3956077848111832 1107 78-79 0.5237410672925833 1107 80-81 0.5343195171413697 1107 82-83 0.5429927092204423 1107 84-85 0.6089588795999177 1107 86-87 0.5348975661551094 1107 88-89 0.5871994169913037 1107 90-91 0.5676239476184044 1107 92-93 0.5205544338021788 1107 94-95 0.4571325916479445 1107 96-97 0.6043927921645782 1107 98-99 0.8628968227739762 1107 100-101 0.6919930467936766 1108 1 0.01936685878257549 1108 2 -0.05995600491802122 1108 3 -0.07233767755771225 1108 4 0.03164659855249141 1108 5 0.03308204702688755 1108 6 -0.03800412480337201 1108 7 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-0.6528228476702225 2316 62-63 -0.4906131666427811 2316 64-65 -0.4998191851965217 2316 66-67 -0.5057370265895713 2316 68-69 -0.5027983977704409 2316 70-71 -0.37573692609618803 2316 72-73 -0.5039806090833707 2316 74-75 -0.4612574982215847 2316 76-77 -1.0005500250905826 2316 78-79 -0.6974065981262498 2316 80-81 -0.4875569384325402 2316 82-83 -0.3790189061187732 2316 84-85 -0.27596724381249516 2316 86-87 -0.24953179636632683 2316 88-89 -0.37698005747572694 2316 90-91 -0.3719582496953464 2316 92-93 -0.34242960429456915 2316 94-95 -0.2540552916766252 2316 96-97 -0.34923285728859454 2316 98-99 -0.27901568040437397 2316 100-101 -0.10516736054575304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 10.0 4 6.0 5 11.0 6 27.0 7 86.0 8 229.0 9 498.0 10 951.0 11 1796.0 12 2517.0 13 3557.0 14 4611.0 15 5930.0 16 7399.0 17 10871.0 18 11180.0 19 6820.0 20 4903.0 21 4889.0 22 5184.0 23 5391.0 24 6073.0 25 7004.0 26 7836.0 27 8026.0 28 10155.0 29 13009.0 30 16571.0 31 21274.0 32 27536.0 33 36529.0 34 51612.0 35 79415.0 36 136828.0 37 299950.0 38 537866.0 39 182357.0 40 197.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.7318936002107 20.47458519884119 15.135633394785355 23.657887806162762 2 15.619449860641721 21.83402606533053 39.8149964518605 22.73152762216725 3 17.660793998437203 26.020903565402804 32.33186598914731 23.98643644701268 4 12.98744651964535 19.76998673937405 37.98886212767862 29.253704613301974 5 13.189665500629976 36.82738400085314 35.975303895844 14.007646602672889 6 28.518878866912512 37.63766320454267 19.22235841343527 14.621099515109545 7 24.20107615926736 33.55532793629938 24.204828366157464 18.038767538275803 8 23.433256138807955 34.756165797515116 22.575317324794977 19.235260738881948 9 24.44088825927881 16.663550798956756 22.52015330069133 36.3754076410731 10-11 22.705408776878492 26.824742200177077 30.267920913959205 20.201928108985225 12-13 23.931838857854554 25.35866489896031 29.48694824455963 21.222547998625508 14-15 21.40400340726717 27.042879167089062 27.74269866618919 23.810418759454574 16-17 20.490820969825634 29.429344919236055 28.803344112830892 21.27648999810742 18-19 20.302889071452647 27.809320494186046 31.167253854903944 20.720536579457363 20-21 20.912563046949984 27.044755270534115 31.283959118060228 20.758722564455674 22-23 20.67544990277176 27.28486359740532 30.880333564609714 21.15935293521321 24-25 20.60764686598565 27.44982904418783 31.200291487229993 20.742232602596527 26-27 20.38585346586795 27.611842538345073 31.369133039299584 20.633170956487394 28-29 20.155685339054546 27.326203542995163 31.49285512323526 21.025255994715035 30-31 20.539350117766634 27.15603782751171 31.36769257708152 20.93691947764014 32-33 20.373417979832823 27.439204214068575 31.222064087862435 20.96531371823617 34-35 20.574361290441512 27.67870897913125 31.176123592916554 20.570806137510683 36-37 20.605647664471192 27.500419674332715 31.026177803671413 20.86775485752468 38-39 20.4612449690805 27.474909584979585 31.194992317850108 20.86885312808981 40-41 20.682756831978807 27.455523182714042 31.019691845072035 20.84202814023511 42-43 20.818461647837612 27.370703558540356 30.89251177995479 20.918323013667248 44-45 20.73851330980195 27.679635259159006 30.62834324925318 20.953508181785864 46-47 20.949723060800235 27.54185685528199 30.265333689683278 21.243086394234503 48-49 20.866154172256575 27.78252472177715 30.18982875454379 21.16149235142248 50-51 20.891629682194658 28.014174126097853 29.926680560803526 21.167515630903967 52-53 20.931027854540762 28.103700465931936 29.93513948335402 21.030132196173277 54-55 20.89436155212342 28.20629370169034 29.706254863057612 21.19308988312863 56-57 21.03760369585796 28.415199466001713 29.423917751624966 21.12327908651536 58-59 20.778565487559586 28.532326510111407 29.745675321892577 20.94343268043643 60-61 21.059469245011314 28.56047726805184 29.493256531577867 20.88679695535898 62-63 20.76000618854658 28.681642071583052 29.519301352592443 21.03905038727793 64-65 20.814270084380798 28.56166223192409 29.49496598567804 21.129101698017074 66-67 20.766637295229682 28.680895520521222 29.270968457578245 21.281498726670854 68-69 20.79551831792097 28.59363196950771 29.376116018162396 21.234733694408924 70-71 20.803855959734133 28.521140436165286 29.324838407928887 21.350165196171687 72-73 20.889633375224403 28.452343255745976 29.237488142017977 21.420535227011644 74-75 20.95735593990234 28.39514097012727 29.18985995974398 21.457643130226412 76-77 20.858484172539445 28.499270448747254 29.150024241574446 21.49222113713885 78-79 20.938151910383368 28.457375552385532 29.048550681228864 21.555921856002232 80-81 20.863509694289682 28.50021099588081 29.028786948143733 21.607492361685782 82-83 20.963125526473707 28.461427808213628 28.96704007141773 21.60840659389493 84-85 21.012929296520007 28.455018022546042 28.880212524838395 21.651840156095556 86-87 20.918920520188404 28.40747433454131 28.88322751166013 21.79037763361015 88-89 20.98494234063198 28.276196206423037 28.862005906151957 21.876855546793024 90-91 20.95647734801829 28.272723652175575 28.90720888048921 21.863590119316928 92-93 21.05738506726983 28.202409838418124 28.88794462005943 21.85226047425262 94-95 21.12278537508245 28.196189074363474 28.87609554566962 21.80493000488445 96-97 21.128907396850515 28.103568809549827 28.70369151329795 22.063832280301703 98-99 21.035346470454645 28.075972365307205 28.690347276850513 22.19833388738764 100-101 20.937179082075993 28.28419174763673 28.50962424625431 22.269004924032966 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1425.0 1 853.5 2 289.0 3 285.5 4 229.5 5 232.5 6 358.5 7 730.5 8 1222.5 9 1521.5 10 1378.0 11 1097.0 12 1108.0 13 1216.0 14 1372.0 15 1543.5 16 1668.5 17 1757.0 18 1893.0 19 2131.0 20 2455.0 21 2767.5 22 3244.0 23 4074.5 24 5162.5 25 6610.5 26 9006.0 27 12260.0 28 15974.5 29 20361.5 30 25284.0 31 29823.5 32 34372.5 33 39156.0 34 43242.0 35 45853.5 36 48623.0 37 51685.5 38 53500.0 39 55082.5 40 57199.0 41 59238.0 42 61096.5 43 63013.5 44 64837.5 45 65309.0 46 64364.5 47 63026.5 48 60653.5 49 57397.5 50 53257.0 51 48786.0 52 44016.0 53 39087.0 54 33752.0 55 28404.0 56 23928.0 57 19948.0 58 16965.0 59 14489.5 60 11780.5 61 9528.0 62 7566.5 63 5467.0 64 3899.0 65 2898.0 66 2131.0 67 1650.0 68 1297.5 69 1014.5 70 773.5 71 577.5 72 459.5 73 345.0 74 256.0 75 181.0 76 130.0 77 90.0 78 58.5 79 35.5 80 17.5 81 9.0 82 7.0 83 6.0 84 7.0 85 4.5 86 3.0 87 2.5 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 1.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020143426462669492 2 0.0 3 0.002303986686906641 4 0.021328333901650048 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.582819105447547E-5 12-13 0.0 14-15 0.0 16-17 0.001217821534507796 18-19 1.3165638210895094E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.9496914632685277E-4 28-29 0.0052991693798852746 30-31 0.0 32-33 2.633127642179019E-4 34-35 0.012112387154023485 36-37 0.004673801564867758 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.962268597451396E-4 60-61 0.0028635263108696828 62-63 0.011256620670315303 64-65 0.0026331276421790186 66-67 0.0037192927945778635 68-69 0.002205244400324928 70-71 1.9748457316342638E-4 72-73 0.0057270526217393656 74-75 0.005167512997776323 76-77 0.0014153061076712223 78-79 8.55766483708181E-4 80-81 0.00783355473548258 82-83 0.010236283708970933 84-85 0.0024027289734883544 86-87 0.002698955833233494 88-89 7.899382926537055E-4 90-91 3.2914095527237735E-5 92-93 0.0 94-95 0.0 96-97 0.0 98-99 6.582819105447547E-5 100-101 0.001711532967416362 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1519106.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.55679381467573 #Duplication Level Percentage of deduplicated Percentage of total 1 63.10926561062197 35.06148457324931 2 20.780832270916065 23.09032827545287 3 8.382099131531586 13.970476595540186 4 3.5623183502768456 7.91643944354266 5 1.6782218089871612 4.661831150859592 6 0.8750308459708269 2.9168344974649507 7 0.4967926089744611 1.9320143181814273 8 0.3081844025640189 1.3697389848118575 9 0.2003573152220817 1.0018089045949667 >10 0.58636856480577 5.028069761264821 >50 0.012338663205468028 0.45545189027277916 >100 0.006766106755544044 0.7072661848850801 >500 4.747733894084827E-4 0.21299336498029922 >1k 7.12160084112724E-4 0.8852742801425749 >5k 2.3738669470424134E-4 0.7899877747566515 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6029 0.3968781638674325 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5951 0.3917435649651834 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 4457 0.2933962475297971 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2803 0.18451641952569472 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 1816 0.11954399495492744 RNA PCR Primer, Index 39 (95% over 23bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1543 0.10157289879705562 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.291409552723773E-5 0.0 18-19 0.0 0.0 0.0 6.582819105447546E-5 0.0 20-21 0.0 0.0 0.0 1.6457047763618863E-4 0.0 22-23 0.0 0.0 0.0 2.633127642179018E-4 0.0 24-25 0.0 0.0 0.0 4.93711432908566E-4 0.0 26-27 0.0 0.0 0.0 9.215946747626564E-4 0.0 28-29 0.0 0.0 0.0 0.001974845731634264 0.0 30-31 0.0 0.0 0.0 0.00536499757093975 0.0 32-33 0.0 0.0 0.0 0.012803583160095477 0.0 34-35 0.0 0.0 0.0 0.024850142123064488 0.0 36-37 0.0 0.0 0.0 0.043150379236208666 0.0 38-39 0.0 0.0 0.0 0.05875166051611935 0.0 40-41 0.0 0.0 0.0 0.07441876998708452 0.0 42-43 0.0 0.0 0.0 0.09502299378713533 0.0 44-45 0.0 0.0 0.0 0.1238886555645228 0.0 46-47 0.0 0.0 0.0 0.1553545308885621 0.0 48-49 0.0 0.0 0.0 0.18422019266594958 0.0 50-51 0.0 0.0 0.0 0.21272379939253744 0.0 52-53 0.0 0.0 0.0 0.2434655646149775 0.0 54-55 0.0 0.0 0.0 0.281053461707083 0.0 56-57 0.0 0.0 0.0 0.3303258627113579 0.0 58-59 0.0 0.0 0.0 0.3759447991121094 0.0 60-61 0.0 0.0 0.0 0.4182064977690826 0.0 62-63 0.0 0.0 0.0 0.4669851873404489 0.0 64-65 0.0 0.0 0.0 0.5228404074501714 0.0 66-67 0.0 0.0 0.0 0.5892939663196644 0.0 68-69 0.0 0.0 0.0 0.6656217538473286 0.0 70-71 0.0 0.0 0.0 0.7376048807653975 0.0 72-73 0.0 0.0 0.0 0.8158745999291689 0.0 74-75 0.0 0.0 0.0 0.9094164594175784 6.582819105447546E-5 76-77 0.0 0.0 0.0 1.0176709196066633 6.582819105447546E-5 78-79 0.0 0.0 0.0 1.1281964523871277 6.582819105447546E-5 80-81 0.0 0.0 0.0 1.2398739785110453 6.582819105447546E-5 82-83 0.0 0.0 0.0 1.3654741670429846 6.582819105447546E-5 84-85 0.0 0.0 0.0 1.5305054420165545 6.582819105447546E-5 86-87 0.0 0.0 0.0 1.716535909936502 6.582819105447546E-5 88-89 0.0 0.0 0.0 1.914777507297055 6.582819105447546E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2835 0.0 57.992134 1 GTATCAA 5025 0.0 39.904556 1 TCAACGC 6050 0.0 33.14059 4 ATCAACG 6085 0.0 32.94997 3 TATCAAC 6110 0.0 32.892914 2 CAACGCA 6195 0.0 32.50652 5 GTGGTAT 1290 0.0 32.046135 1 AACGCAG 6490 0.0 31.248493 6 TGGTATC 1210 0.0 31.020224 2 CGCAGAG 7005 0.0 28.95114 8 ACGCAGA 7085 0.0 28.691273 7 GCAGAGT 8105 0.0 24.963327 9 TAAGACG 115 1.2798248E-4 24.788862 4 GAGTACT 6175 0.0 23.074389 12-13 CAGAGTA 7850 0.0 22.05331 10-11 AGAGTAC 7450 0.0 20.623573 10-11 GTACTTT 6990 0.0 20.180187 14-15 GTACATG 2955 0.0 19.296114 1 AGTACTT 6830 0.0 18.566774 12-13 TACATGG 3040 0.0 17.816994 2 >>END_MODULE