FastQCFastQC Report
Fri 10 Feb 2017
SRR2031716_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031716_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978996
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10310.10531197267404566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12600.046.75851
GTATCAA19600.033.9376221
TCAACGC19550.032.5645644
ATCAACG20550.030.979913
CAACGCA20600.030.9047155
AACGCAG21850.029.1247866
TATCAAC22650.028.1090412
ACGCAGA24900.025.7493387
CGCAGAG25650.024.8150838
GTGGTAT6850.023.5829321
GTATAGC3007.2759576E-1222.1725771
TGGTATC7300.021.478332
GTACATG20400.021.427281
GAGTACT14350.020.52707112-13
ACATGGG20650.020.2464923
GCAGAGT30300.019.595929
TACATGG21800.018.7435282
CAGAGTA28050.018.46114310-11
AGAGTAC23700.017.13889110-11
GTACTTT17350.016.15621814-15
Produced by FastQC (version 0.11.5)