##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031716_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 978996 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80413811700967 33.0 31.0 34.0 30.0 34.0 2 32.109153663549186 34.0 31.0 34.0 31.0 34.0 3 32.221613775745766 34.0 31.0 34.0 31.0 34.0 4 35.621384561326096 37.0 35.0 37.0 35.0 37.0 5 35.61592897213063 37.0 37.0 37.0 35.0 37.0 6 35.63653784080834 37.0 37.0 37.0 35.0 37.0 7 35.61578086120883 37.0 37.0 37.0 35.0 37.0 8 35.60440185659594 37.0 37.0 37.0 35.0 37.0 9 37.37394228372741 39.0 38.0 39.0 35.0 39.0 10-11 37.29703032494515 39.0 38.0 39.0 35.0 39.0 12-13 37.24834268985777 39.0 38.0 39.0 35.0 39.0 14-15 38.654816771467914 41.0 39.0 41.0 35.5 41.0 16-17 38.57855445783231 41.0 39.0 41.0 35.0 41.0 18-19 38.56717034594625 41.0 39.0 41.0 35.0 41.0 20-21 38.55710697490082 41.0 39.0 41.0 35.0 41.0 22-23 38.459397689061035 41.0 39.0 41.0 34.5 41.0 24-25 38.38896532774393 41.0 39.0 41.0 34.0 41.0 26-27 38.27965078509003 40.5 38.5 41.0 34.0 41.0 28-29 38.1683643242669 40.0 38.0 41.0 34.0 41.0 30-31 38.029743226734325 40.0 38.0 41.0 34.0 41.0 32-33 37.85705150991424 40.0 38.0 41.0 33.5 41.0 34-35 37.702430857735884 40.0 38.0 41.0 33.0 41.0 36-37 37.55294761163478 40.0 38.0 41.0 33.0 41.0 38-39 37.37906436798516 40.0 38.0 41.0 32.5 41.0 40-41 37.29208750597551 40.0 38.0 41.0 32.0 41.0 42-43 37.28577389488824 40.0 38.0 41.0 32.0 41.0 44-45 37.18789045103351 40.0 37.5 41.0 31.5 41.0 46-47 37.16378207878275 40.0 37.5 41.0 31.5 41.0 48-49 37.253384079199506 40.0 38.0 41.0 32.0 41.0 50-51 36.7222812963485 39.5 37.0 40.5 31.0 41.0 52-53 36.74478853846185 39.5 37.0 40.5 31.0 41.0 54-55 36.96865768603753 40.0 37.0 41.0 31.5 41.0 56-57 36.83788646736044 40.0 37.0 41.0 31.0 41.0 58-59 36.651310628439745 40.0 36.0 41.0 31.0 41.0 60-61 36.41901601232283 39.5 36.0 41.0 31.0 41.0 62-63 36.136773286101274 39.0 35.0 41.0 30.0 41.0 64-65 35.86110259898916 39.0 35.0 41.0 30.0 41.0 66-67 35.57594719488129 38.0 35.0 41.0 29.5 41.0 68-69 35.240379940265335 37.5 35.0 40.0 29.0 41.0 70-71 34.878265079734746 37.0 35.0 39.5 29.0 41.0 72-73 34.51824062611083 36.5 35.0 39.0 29.0 41.0 74-75 34.1088303731578 36.0 34.5 39.0 28.0 41.0 76-77 33.72160407192675 35.5 34.0 38.0 28.0 39.5 78-79 33.32718979444247 35.0 34.0 37.0 27.0 39.0 80-81 32.974375789073704 35.0 34.0 37.0 26.0 39.0 82-83 32.64831010545498 35.0 34.0 36.0 26.0 38.0 84-85 32.327734229761916 35.0 34.0 36.0 26.0 37.0 86-87 32.05243994868212 35.0 34.0 35.5 25.5 37.0 88-89 31.78850628603181 35.0 33.0 35.0 25.0 36.5 90-91 31.55572239314563 35.0 33.0 35.0 24.5 36.0 92-93 31.342421215204148 35.0 33.0 35.0 23.5 36.0 94-95 31.09740897817764 35.0 33.0 35.0 21.5 36.0 96-97 30.84137371347789 35.0 33.0 35.0 19.0 35.0 98-99 30.60345292524178 35.0 33.0 35.0 9.5 35.0 100-101 30.022219702634125 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.056322747924337335 1101 2 0.2076519013869813 1101 3 0.3138280826571247 1101 4 0.452512022742404 1101 5 0.3746102336870223 1101 6 0.4445552920056457 1101 7 0.5484141939188873 1101 8 0.4264298552858321 1101 9 0.3441480316301906 1101 10-11 0.22843232902621224 1101 12-13 0.30315582296112353 1101 14-15 0.4580221632269996 1101 16-17 0.24578403689208272 1101 18-19 0.41021821638251055 1101 20-21 0.4704847241621195 1101 22-23 0.4879098683778764 1101 24-25 0.3796504741032365 1101 26-27 0.1551894456026801 1101 28-29 0.07011532923414876 1101 30-31 0.1438885730029753 1101 32-33 0.15702302345621177 1101 34-35 0.03165415148659889 1101 36-37 -0.103507886723051 1101 38-39 0.26726874209035856 1101 40-41 0.0020989378620654975 1101 42-43 -0.09463774425751836 1101 44-45 -0.4023786060573613 1101 46-47 -0.21996980260248478 1101 48-49 -0.07007739803306379 1101 50-51 0.1355128884488792 1101 52-53 -0.11125907817643821 1101 54-55 0.0036788957684166235 1101 56-57 -0.2000771581958105 1101 58-59 -0.07408934997975081 1101 60-61 -0.16445705375591757 1101 62-63 0.03555079930313809 1101 64-65 0.13771626386699864 1101 66-67 -0.1844671048513007 1101 68-69 -0.074235389623027 1101 70-71 -0.12344599748229257 1101 72-73 0.2591721401767302 1101 74-75 0.21187807481818766 1101 76-77 0.005522150898727318 1101 78-79 -0.060261875386260044 1101 80-81 -0.40887697012662017 1101 82-83 -0.24648859196014428 1101 84-85 -0.24695727034228554 1101 86-87 -0.1810106068712969 1101 88-89 -0.44082105800017857 1101 90-91 -0.7330467605958617 1101 92-93 -0.3578728886115634 1101 94-95 -0.5873025040491093 1101 96-97 -0.5985600023393651 1101 98-99 -0.46633737546321896 1101 100-101 -0.43094756930229394 1102 1 0.05495984026479661 1102 2 0.17893381999136437 1102 3 0.22829754197548624 1102 4 0.22110570223888715 1102 5 0.15415953475409339 1102 6 0.18848111286875735 1102 7 0.14941335153415736 1102 8 0.0500948602831599 1102 9 0.18223768169401922 1102 10-11 0.23159627680760053 1102 12-13 0.26797201522811775 1102 14-15 0.3739222905857247 1102 16-17 0.3972993284080175 1102 18-19 0.31682054148758 1102 20-21 0.2511659315905064 1102 22-23 0.4062016291775734 1102 24-25 0.055597232924021966 1102 26-27 0.18481151207869573 1102 28-29 0.14177039193560148 1102 30-31 0.037907487389276184 1102 32-33 0.2297021919131339 1102 34-35 0.3051358184535644 1102 36-37 0.17235386361998195 1102 38-39 0.11248555220883105 1102 40-41 0.18879803711100607 1102 42-43 0.2980036323870152 1102 44-45 0.13810899148293032 1102 46-47 -0.008601304711532975 1102 48-49 0.2169840652523618 1102 50-51 0.17114637870908922 1102 52-53 0.16762647916039697 1102 54-55 0.21477558213179293 1102 56-57 0.17040965394006946 1102 58-59 0.13614416153257736 1102 60-61 0.10123678944348313 1102 62-63 0.18062192460286752 1102 64-65 0.1618235593500046 1102 66-67 0.15448803007655698 1102 68-69 0.21730364882637332 1102 70-71 0.21762356932867277 1102 72-73 0.0827910989152798 1102 74-75 -0.13502042475138154 1102 76-77 0.1287436334034524 1102 78-79 0.20574073954843897 1102 80-81 0.09520867283469414 1102 82-83 0.2795922246140421 1102 84-85 0.24963392050020872 1102 86-87 0.22516590584070428 1102 88-89 0.3482478065668424 1102 90-91 0.3225875920692012 1102 92-93 0.5732764044474656 1102 94-95 0.22467629927176702 1102 96-97 0.1643995554933504 1102 98-99 0.27809125822570024 1102 100-101 0.1812582312943043 1103 1 -0.2029892585964177 1103 2 -0.07919417563496722 1103 3 -0.06334568921494821 1103 4 -0.0010611812506624574 1103 5 0.0486261862503099 1103 6 -0.172189077348591 1103 7 0.007478089264338905 1103 8 -0.0506512107860857 1103 9 -0.23602009280159564 1103 10-11 -0.27668664341732097 1103 12-13 -0.08946443706297913 1103 14-15 0.08911577817099925 1103 16-17 0.14365090778021994 1103 18-19 0.017819644141404467 1103 20-21 -0.09536998300847443 1103 22-23 -0.14126469171742428 1103 24-25 -0.08455478639110225 1103 26-27 -0.248304712140488 1103 28-29 -0.19069922781574888 1103 30-31 -0.08504325016630787 1103 32-33 -0.08925642854951121 1103 34-35 -0.27148709722022346 1103 36-37 -0.14140530126129391 1103 38-39 -0.0866837943313783 1103 40-41 -0.033091198599073834 1103 42-43 0.017592255697699954 1103 44-45 -0.02864211445686493 1103 46-47 -0.07411043774918369 1103 48-49 0.11540361967061585 1103 50-51 0.08841878982244822 1103 52-53 0.06096321868813703 1103 54-55 -0.20327896563865266 1103 56-57 -0.06709583819061748 1103 58-59 0.14926445028626745 1103 60-61 0.26358846613552345 1103 62-63 0.2434290009001927 1103 64-65 0.19423321290568651 1103 66-67 0.20760677316219756 1103 68-69 0.12313167608165543 1103 70-71 0.19603072977903935 1103 72-73 0.023264989290204596 1103 74-75 -0.007371142556756638 1103 76-77 -0.10108504074253233 1103 78-79 -0.029353224188469085 1103 80-81 0.01056975090953216 1103 82-83 -0.2729948372856654 1103 84-85 -0.37777993355151906 1103 86-87 -0.17577967090100088 1103 88-89 -0.1429197023457931 1103 90-91 -0.14650119367973957 1103 92-93 -0.6648724004505873 1103 94-95 -0.3135024171654095 1103 96-97 -0.37463070954578725 1103 98-99 -0.16394404311516553 1103 100-101 0.0327717694859011 1104 1 -0.1836110658024559 1104 2 0.005243646964203208 1104 3 0.11890865072739132 1104 4 -0.011903567611426524 1104 5 -0.008977228072261312 1104 6 0.13135790344849596 1104 7 0.03952720069326432 1104 8 0.03074968130395206 1104 9 0.13807609835372148 1104 10-11 0.17210183775889476 1104 12-13 0.1604502447099989 1104 14-15 0.25111218484613573 1104 16-17 0.2356810566522256 1104 18-19 0.12717902044857254 1104 20-21 0.20475298147126608 1104 22-23 0.13353967391997656 1104 24-25 0.20045456873589274 1104 26-27 0.2019477899742057 1104 28-29 -0.08548957072198249 1104 30-31 0.1801045528727485 1104 32-33 0.0951112803938372 1104 34-35 0.0392272786022545 1104 36-37 0.05036019511815937 1104 38-39 -0.007845104872458819 1104 40-41 0.10200706011234928 1104 42-43 0.08594775849095981 1104 44-45 0.04581048778225494 1104 46-47 0.23953397181549008 1104 48-49 0.20499572551035783 1104 50-51 0.037893136590248844 1104 52-53 0.10650190535844217 1104 54-55 0.17967836993619102 1104 56-57 0.11364534813775151 1104 58-59 -0.014497412931014253 1104 60-61 0.007512945229571244 1104 62-63 3.550482561252011E-4 1104 64-65 0.03052579400448252 1104 66-67 -0.04708772118953419 1104 68-69 -0.005570703400543664 1104 70-71 -0.13188074641884384 1104 72-73 -0.24868104975301009 1104 74-75 -0.31835442018426363 1104 76-77 -0.19000456009217714 1104 78-79 -0.30692362666475503 1104 80-81 -0.1385499110576589 1104 82-83 -0.18230919743880492 1104 84-85 -0.12536214114902577 1104 86-87 -0.04145359194080811 1104 88-89 0.14318849842890913 1104 90-91 -0.0012962108297003283 1104 92-93 -0.08746652604166982 1104 94-95 0.10237646680578649 1104 96-97 -0.16773766075704089 1104 98-99 -0.3711338798220538 1104 100-101 -0.36974169884277686 1105 1 0.05903506991510454 1105 2 0.30976851865612787 1105 3 0.25065236024462934 1105 4 0.22542876801191625 1105 5 0.18001755010921272 1105 6 0.2670344465325414 1105 7 0.2516839323146627 1105 8 0.29745272626678343 1105 9 0.2772902028572162 1105 10-11 0.3882112908538815 1105 12-13 0.307538717306322 1105 14-15 0.4220835475033624 1105 16-17 0.42582995492434605 1105 18-19 0.39503494250786275 1105 20-21 0.37326943063465734 1105 22-23 0.26091674490364625 1105 24-25 0.35137022039423726 1105 26-27 0.4807526262159385 1105 28-29 0.30017920364846873 1105 30-31 0.3629284864769673 1105 32-33 0.49868476434492237 1105 34-35 0.4255480034170134 1105 36-37 0.4941650500048027 1105 38-39 0.5465470771913772 1105 40-41 0.3795260188809877 1105 42-43 0.4648335136534243 1105 44-45 0.725123584341766 1105 46-47 0.726584458509862 1105 48-49 0.7990673890964715 1105 50-51 0.7846312091288965 1105 52-53 0.6574578300280223 1105 54-55 0.5594037676632553 1105 56-57 0.5590984132992389 1105 58-59 0.5559001836154422 1105 60-61 0.5520158656443783 1105 62-63 0.6609946433383413 1105 64-65 0.8746692405041472 1105 66-67 0.8507909849529725 1105 68-69 0.7751881196828521 1105 70-71 0.6647902665841201 1105 72-73 0.46858687391223697 1105 74-75 0.5771514100799138 1105 76-77 0.646534248050429 1105 78-79 0.5813678654130214 1105 80-81 0.4525528609436478 1105 82-83 0.5950320131629425 1105 84-85 0.5849046178306736 1105 86-87 0.6234003748886536 1105 88-89 0.5227833817676846 1105 90-91 0.2874109384998924 1105 92-93 0.4074371978445761 1105 94-95 0.6336427422607507 1105 96-97 0.508031037915071 1105 98-99 0.6000236833624477 1105 100-101 0.6583822873400997 1106 1 -0.07230544022450758 1106 2 0.1564578141481192 1106 3 0.20191284362606865 1106 4 0.12527515627795793 1106 5 0.03609326582839856 1106 6 0.07810935702892863 1106 7 0.141733856128198 1106 8 0.09146310744687014 1106 9 0.1755930409578852 1106 10-11 0.16255317606627528 1106 12-13 0.3045079825714936 1106 14-15 0.10971966112649056 1106 16-17 0.1564055034049474 1106 18-19 0.18764733302852932 1106 20-21 0.16776046788550758 1106 22-23 0.25615725875967144 1106 24-25 0.12931083211579875 1106 26-27 0.0069830685929517244 1106 28-29 0.07818485259610242 1106 30-31 0.07482162721752417 1106 32-33 0.13059574386748096 1106 34-35 0.2776923736708454 1106 36-37 0.15949463389043927 1106 38-39 0.20940214947561486 1106 40-41 0.23384015487692267 1106 42-43 0.1583043955582113 1106 44-45 0.044513349340597586 1106 46-47 0.18935219509051393 1106 48-49 -0.03250601625268246 1106 50-51 0.12353214194853734 1106 52-53 0.23055533631495706 1106 54-55 0.1842139209033533 1106 56-57 0.11325645995260913 1106 58-59 0.1535953882039749 1106 60-61 0.09201252087275691 1106 62-63 0.05783259534706531 1106 64-65 0.12591423883176134 1106 66-67 0.11188014169887595 1106 68-69 0.21054978860001938 1106 70-71 0.122430592141896 1106 72-73 0.07352041206120674 1106 74-75 0.06952037555443269 1106 76-77 0.021187692545808545 1106 78-79 0.1200730671522976 1106 80-81 0.22779225987580531 1106 82-83 0.1712382988559895 1106 84-85 0.21276346846804017 1106 86-87 0.17933672512340593 1106 88-89 0.22607488480895555 1106 90-91 0.32275278654815764 1106 92-93 0.3022038690535247 1106 94-95 0.2646840811604463 1106 96-97 0.42893462177592667 1106 98-99 0.4292885768872168 1106 100-101 0.5034338835394472 1107 1 0.11962377458519668 1107 2 0.26568270570192 1107 3 0.2169215325877687 1107 4 0.22597027321413776 1107 5 0.36193596245761483 1107 6 0.20914155261679213 1107 7 0.2097133740566477 1107 8 0.16203737485464131 1107 9 0.22127044833089826 1107 10-11 0.2234329500206158 1107 12-13 0.287583079078658 1107 14-15 0.44279807403125915 1107 16-17 0.4293881387471359 1107 18-19 0.3238842830039914 1107 20-21 0.39638363225943607 1107 22-23 0.3737262066656939 1107 24-25 0.2957780727403403 1107 26-27 0.22644368968789053 1107 28-29 0.21663184992971907 1107 30-31 0.20945211419734733 1107 32-33 0.33822544817747513 1107 34-35 0.361203908101686 1107 36-37 0.1984569994105172 1107 38-39 0.18381083578420032 1107 40-41 0.18533717675187944 1107 42-43 0.3034226582964905 1107 44-45 0.21963108145526888 1107 46-47 0.32629951743109586 1107 48-49 0.34945145426453905 1107 50-51 0.4710892834791238 1107 52-53 0.2470756393010376 1107 54-55 0.24954629027045172 1107 56-57 0.27828305746132287 1107 58-59 0.4161976990698193 1107 60-61 0.27879004365640725 1107 62-63 0.38536068523470135 1107 64-65 0.25570145876198325 1107 66-67 0.26253214546630943 1107 68-69 0.36538973925202356 1107 70-71 0.23097495890893782 1107 72-73 0.16970854466405427 1107 74-75 0.29622931523579865 1107 76-77 0.16361834667206665 1107 78-79 0.1625441813292383 1107 80-81 0.36704580510483 1107 82-83 0.3783791661602933 1107 84-85 0.2566639500089707 1107 86-87 0.2666395652863116 1107 88-89 0.25406793363959324 1107 90-91 0.1300913111491937 1107 92-93 0.12061451866948758 1107 94-95 0.20896793826350546 1107 96-97 0.31650741670316407 1107 98-99 0.4179100167343961 1107 100-101 0.3775819009969652 1108 1 -0.38952616638292525 1108 2 -0.31843771932759424 1108 3 -0.2658116433272255 1108 4 -0.16541107271266497 1108 5 -0.2764937896085016 1108 6 -0.18017367760752734 1108 7 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-0.10576023302732551 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10811.0 3 1327.0 4 1147.0 5 1060.0 6 1066.0 7 1126.0 8 1311.0 9 1423.0 10 1421.0 11 1677.0 12 1906.0 13 2227.0 14 2494.0 15 2677.0 16 2704.0 17 3297.0 18 3281.0 19 2492.0 20 2423.0 21 2589.0 22 2937.0 23 3372.0 24 3914.0 25 4438.0 26 5467.0 27 6548.0 28 7851.0 29 9514.0 30 12043.0 31 15299.0 32 19565.0 33 26260.0 34 37773.0 35 59152.0 36 104903.0 37 207325.0 38 301583.0 39 102523.0 40 70.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.00679576297885 19.955444997688375 13.96179480150726 24.07596443782552 2 16.098847313244594 21.863303973184102 38.739849165685555 23.297999547885752 3 18.539482714709603 25.697236624215876 30.657443481053637 25.105837180020885 4 13.827866647436862 18.656798094203545 37.25836164103886 30.25697361732074 5 14.297695206827957 36.64697850666585 34.79506415271876 14.26026213378744 6 30.13752279008985 36.596973046596595 18.89724085252064 14.368263310792917 7 25.91113238109262 32.47281941785697 23.631256237831117 17.984791963219294 8 24.290134376003323 34.353815227371726 22.11109997730066 19.244950419324297 9 25.313801114101313 16.66540559995419 21.871804729613665 36.14898855633083 10-11 23.865994769590014 26.244931634574687 29.581056019367647 20.308017576467655 12-13 24.628625740128257 24.70419155063804 28.72569355149595 21.941489157737752 14-15 22.372692932535678 26.59760570719387 26.357470748892776 24.67223061137767 16-17 22.045431183014013 28.68934662948327 27.336240566281383 21.928981621221336 18-19 21.959898293365953 28.081678282197043 28.548174619571775 21.410248804865226 20-21 22.771669681085253 27.569171830040993 28.103278736306642 21.555879752567115 22-23 22.684110226581865 27.401612810033356 27.7222113424386 22.19206562094618 24-25 22.402430252181606 27.666562202359803 27.93951567757032 21.991491867888268 26-27 22.262618102989897 27.67163278661704 28.023078653523665 22.042670456869402 28-29 22.259506857719888 27.50332979688878 28.21346490611379 22.023698439277542 30-31 22.608376013668614 27.479046673490064 27.89806207931027 22.01451523353105 32-33 22.529036849281383 27.666689331402228 27.681208395086216 22.12306542423017 34-35 22.61660060458058 27.573967038495994 27.72761763346382 22.08181472345961 36-37 22.652910018821817 27.433017897856992 27.808380490144497 22.105691593176694 38-39 22.5015069411319 27.593152991207848 27.78922149810655 22.116118569553706 40-41 22.67591793495822 27.431960129085564 27.7493568217793 22.142765114176914 42-43 22.608448918450502 27.42897458575964 27.624936732805384 22.337639762984473 44-45 22.575901367083638 27.56522486112361 27.54512971341149 22.313744058381264 46-47 22.644279221307137 27.397009519163856 27.551375720379866 22.40733553914914 48-49 22.50868974009789 27.52319522651111 27.566560826646523 22.401554206744475 50-51 22.545751669077543 27.611095856719846 27.500497259067114 22.342655215135494 52-53 22.600920245398772 27.609406952965237 27.367484662576686 22.422188139059305 54-55 22.542985893316768 27.582744809359127 27.44001656301999 22.43425273430411 56-57 22.538886860735392 27.601567029791358 27.4621868675868 22.39735924188645 58-59 22.49013662254029 27.48371270973577 27.56050313577948 22.46564753194446 60-61 22.586300243712778 27.56607775802649 27.5102492802881 22.337372717972627 62-63 22.427317340515327 27.606910623347712 27.503996670761754 22.461775365375207 64-65 22.538407571266987 27.58012358330214 27.358606343615577 22.522862501815297 66-67 22.44960151102153 27.536516438852765 27.430368608057314 22.58351344206839 68-69 22.43413725712778 27.57087384399522 27.44876660438584 22.546222294491162 70-71 22.45828787273008 27.421614331159066 27.557050287692764 22.563047508418094 72-73 22.432476789255798 27.508087140181697 27.39688416945185 22.66255190111065 74-75 22.48579362466542 27.556891957934 27.32546964364951 22.631844773751077 76-77 22.565618955240595 27.53017681951892 27.307394758869226 22.59680946637126 78-79 22.482470444041176 27.53565608578425 27.334346782758267 22.647526687416303 80-81 22.460165117518144 27.538761097061 27.314800137853595 22.686273647567262 82-83 22.375617012333343 27.54177377399081 27.373231546645954 22.709377667029898 84-85 22.525461851578587 27.464030772550334 27.314831148259326 22.695676227611745 86-87 22.40972431380149 27.49805302023478 27.41894658039786 22.67327608556586 88-89 22.499339163014806 27.29397352459515 27.370257376193408 22.836429936196637 90-91 22.43950753132641 27.446466683506348 27.464363107187307 22.649662677979933 92-93 22.434139670844104 27.37705236608289 27.461637515303572 22.72717044776944 94-95 22.463780712769395 27.259564059668772 27.476757075134056 22.799898152427776 96-97 22.474057207825037 27.259575151152966 27.49616462292147 22.770203018100528 98-99 22.447596134674942 27.294027068727 27.341520547959213 22.916856248638844 100-101 22.46393674450005 27.293612820899682 27.45732631971952 22.785124114880745 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1461.0 1 838.0 2 206.5 3 187.0 4 152.5 5 142.0 6 186.0 7 314.5 8 462.5 9 490.0 10 366.0 11 263.5 12 265.5 13 266.5 14 274.0 15 285.0 16 299.5 17 327.5 18 352.5 19 394.5 20 465.5 21 542.5 22 606.0 23 700.5 24 872.0 25 1120.5 26 1518.0 27 2150.0 28 3204.5 29 5033.5 30 7798.0 31 11818.5 32 16906.0 33 22329.0 34 26954.5 35 30533.0 36 34166.0 37 36974.0 38 37699.0 39 37073.5 40 36116.0 41 35119.5 42 34640.0 43 34783.5 44 35038.5 45 35290.0 46 35603.5 47 35924.5 48 35923.5 49 35611.5 50 34990.5 51 33530.0 52 31848.0 53 30598.0 54 29048.0 55 26990.0 56 24455.0 57 22056.0 58 20078.5 59 17547.5 60 14855.0 61 12503.5 62 9995.5 63 7553.0 64 5614.0 65 4252.0 66 3223.5 67 2434.5 68 1865.5 69 1462.5 70 1168.0 71 882.0 72 641.5 73 472.0 74 370.0 75 298.5 76 225.5 77 161.0 78 118.0 79 99.5 80 80.0 81 58.0 82 52.0 83 40.0 84 25.5 85 19.5 86 14.0 87 12.0 88 10.5 89 3.5 90 3.5 91 4.0 92 3.5 93 4.5 94 3.5 95 2.5 96 1.5 97 0.5 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13564917527752923 2 0.13963284834667353 3 0.13432128425448112 4 0.13758993908044567 5 0.127783974602552 6 0.10715059101365071 7 0.1115428459360406 8 0.10153259053152412 9 0.10091971775165577 10-11 0.08886655308091146 12-13 0.10628235457550389 14-15 0.12385137426506337 16-17 0.13478093883938239 18-19 0.13095048396520517 20-21 0.12211490138876971 22-23 0.11619046451670895 24-25 0.10117508140993427 26-27 0.10582269999060262 28-29 0.11067460949789375 30-31 0.10102186321496719 32-33 0.09923431760701781 34-35 0.11522008261525073 36-37 0.11659904636995454 38-39 0.10434159077258742 40-41 0.10623128184384818 42-43 0.10224760877470389 44-45 0.11726299188147857 46-47 0.11506686442028363 48-49 0.128958647430633 50-51 0.11598617359008616 52-53 0.10173688145814691 54-55 0.09330988073495704 56-57 0.11184928232597477 58-59 0.1031158452128507 60-61 0.10219653604304818 62-63 0.10158366326317983 64-65 0.12196168319380263 66-67 0.11384111886054693 68-69 0.11920375568439502 70-71 0.10653771823378236 72-73 0.10771239106186338 74-75 0.09336095346661273 76-77 0.11588402812677477 78-79 0.11721191914982289 80-81 0.11716084641816718 82-83 0.1225234832420153 84-85 0.11353468247061275 86-87 0.1225234832420153 88-89 0.10924457301153426 90-91 0.11716084641816718 92-93 0.13105262942851656 94-95 0.10868277296332161 96-97 0.0950463536112507 98-99 0.09877466302211653 100-101 0.14029679385819757 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 978996.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.50800360058325 #Duplication Level Percentage of deduplicated Percentage of total 1 67.5811553765107 40.89200792853485 2 19.0336593412369 23.033774559036743 3 6.996577716386786 12.700468489646767 4 2.90611621643531 7.033731619511244 5 1.3322299035661112 4.030528590089146 6 0.742141592610336 2.6943303694685277 7 0.4243631163727335 1.797415548140526 8 0.2537448901871109 1.2282877383257047 9 0.17141029153834134 0.9334525083821084 >10 0.5502922821425754 4.98768019013658 >50 0.006614678501423887 0.2487432165438414 >100 0.001186216557507847 0.15626563787290085 >500 3.389186361320567E-4 0.1575985240756203 >1k 1.6945931806602835E-4 0.10571508023550066 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1031 0.10531197267404566 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.0214546331139248E-4 0.0 0.0 0.0 3 0.0 1.0214546331139248E-4 0.0 0.0 0.0 4 0.0 1.0214546331139248E-4 0.0 0.0 0.0 5 0.0 1.0214546331139248E-4 0.0 0.0 0.0 6 0.0 1.0214546331139248E-4 0.0 0.0 0.0 7 0.0 1.0214546331139248E-4 0.0 0.0 0.0 8 0.0 1.0214546331139248E-4 0.0 0.0 0.0 9 0.0 1.0214546331139248E-4 0.0 0.0 0.0 10-11 0.0 1.0214546331139248E-4 0.0 0.0 0.0 12-13 0.0 1.0214546331139248E-4 0.0 0.0 1.0214546331139248E-4 14-15 0.0 1.0214546331139248E-4 0.0 0.0 1.0214546331139248E-4 16-17 0.0 1.0214546331139248E-4 0.0 1.0214546331139248E-4 1.0214546331139248E-4 18-19 0.0 1.0214546331139248E-4 0.0 2.0429092662278497E-4 1.0214546331139248E-4 20-21 0.0 1.0214546331139248E-4 0.0 2.0429092662278497E-4 1.0214546331139248E-4 22-23 0.0 1.0214546331139248E-4 0.0 2.0429092662278497E-4 1.0214546331139248E-4 24-25 0.0 1.0214546331139248E-4 0.0 4.0858185324556994E-4 1.0214546331139248E-4 26-27 0.0 1.0214546331139248E-4 0.0 0.0011746728280810136 1.0214546331139248E-4 28-29 0.0 1.0214546331139248E-4 0.0 0.0029111457043746857 1.0214546331139248E-4 30-31 0.0 1.0214546331139248E-4 0.0 0.007354473358420259 1.0214546331139248E-4 32-33 0.0 1.0214546331139248E-4 0.0 0.01716043783631394 1.0214546331139248E-4 34-35 0.0 1.0214546331139248E-4 0.0 0.033810148356070914 1.0214546331139248E-4 36-37 0.0 1.0214546331139248E-4 0.0 0.0598061687688203 1.0214546331139248E-4 38-39 0.0 1.0214546331139248E-4 0.0 0.08723222566792918 1.0214546331139248E-4 40-41 0.0 1.0214546331139248E-4 0.0 0.11905053748942795 1.0214546331139248E-4 42-43 0.0 1.0214546331139248E-4 0.0 0.15541432242828368 1.0214546331139248E-4 44-45 0.0 1.0214546331139248E-4 0.0 0.20229909008821284 1.5321819496708872E-4 46-47 0.0 1.0214546331139248E-4 0.0 0.253729330865499 2.0429092662278497E-4 48-49 0.0 1.0214546331139248E-4 0.0 0.3032698805715243 2.0429092662278497E-4 50-51 0.0 1.0214546331139248E-4 0.0 0.3523507756926484 2.0429092662278497E-4 52-53 0.0 1.0214546331139248E-4 0.0 0.4017381072037067 4.0858185324556994E-4 54-55 0.0 1.0214546331139248E-4 0.0 0.4615953487041826 4.0858185324556994E-4 56-57 0.0 1.0214546331139248E-4 0.0 0.5359061732632207 4.0858185324556994E-4 58-59 0.0 1.0214546331139248E-4 0.0 0.6090423249941777 4.0858185324556994E-4 60-61 0.0 1.0214546331139248E-4 0.0 0.6817188221402335 4.0858185324556994E-4 62-63 0.0 1.0214546331139248E-4 0.0 0.7603708288900057 5.107273165569625E-4 64-65 0.0 1.0214546331139248E-4 0.0 0.8451515634384614 5.107273165569625E-4 66-67 0.0 1.0214546331139248E-4 0.0 0.9538854091334388 5.107273165569625E-4 68-69 0.0 1.0214546331139248E-4 0.0 1.0803925654445985 5.107273165569625E-4 70-71 0.0 1.0214546331139248E-4 0.0 1.2019967395168112 5.107273165569625E-4 72-73 0.0 1.0214546331139248E-4 0.0 1.3387184421591098 5.107273165569625E-4 74-75 0.0 1.0214546331139248E-4 0.0 1.482641399964862 5.107273165569625E-4 76-77 0.0 1.0214546331139248E-4 0.0 1.6525093054517077 5.107273165569625E-4 78-79 0.0 1.0214546331139248E-4 0.0 1.8339707210243965 5.107273165569625E-4 80-81 0.0 1.0214546331139248E-4 0.0 2.0328479380916775 5.107273165569625E-4 82-83 1.0214546331139248E-4 1.0214546331139248E-4 0.0 2.230141900477632 5.107273165569625E-4 84-85 1.0214546331139248E-4 1.0214546331139248E-4 0.0 2.4668129389701283 5.107273165569625E-4 86-87 1.0214546331139248E-4 1.0214546331139248E-4 0.0 2.746589362980033 5.618000482126587E-4 88-89 1.0214546331139248E-4 1.0214546331139248E-4 0.0 3.0627295719287924 6.128727798683549E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1260 0.0 46.7585 1 GTATCAA 1960 0.0 33.937622 1 TCAACGC 1955 0.0 32.564564 4 ATCAACG 2055 0.0 30.97991 3 CAACGCA 2060 0.0 30.904715 5 AACGCAG 2185 0.0 29.124786 6 TATCAAC 2265 0.0 28.109041 2 ACGCAGA 2490 0.0 25.749338 7 CGCAGAG 2565 0.0 24.815083 8 GTGGTAT 685 0.0 23.582932 1 GTATAGC 300 7.2759576E-12 22.172577 1 TGGTATC 730 0.0 21.47833 2 GTACATG 2040 0.0 21.42728 1 GAGTACT 1435 0.0 20.527071 12-13 ACATGGG 2065 0.0 20.246492 3 GCAGAGT 3030 0.0 19.59592 9 TACATGG 2180 0.0 18.743528 2 CAGAGTA 2805 0.0 18.461143 10-11 AGAGTAC 2370 0.0 17.138891 10-11 GTACTTT 1735 0.0 16.156218 14-15 >>END_MODULE