FastQCFastQC Report
Fri 10 Feb 2017
SRR2031716_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031716_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978996
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT54680.5585313933866941No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT27370.2795721330832812TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT17680.1805931791345419RNA PCR Primer, Index 11 (95% over 23bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG16330.16680354158750393TruSeq Adapter, Index 11 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14410.14719161263171657No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13820.14116503029634442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.049.038351
GTATCAA20950.037.4245031
ATCAACG24400.032.5141073
TCAACGC24550.032.5089534
CAACGCA24950.031.9812365
TATCAAC25700.030.8694272
AACGCAG25900.030.8081766
ACGCAGA29750.026.5019367
CGCAGAG30300.026.020888
GCAGAGT34800.021.8372149
GTACATG23400.021.1190171
TACATGG23500.020.417322
GTGGTAT7250.020.317951
ACATGGG23950.019.6369883
GAGTACT18550.019.0751912-13
AGAGTAC29950.018.63363310-11
CAGAGTA34450.018.33660310-11
GGCCCGT1350.00940406117.591096
CATGGGG16100.017.4089014
TGGTATC8400.016.9663032
Produced by FastQC (version 0.11.5)