##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031716_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	978996
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73358215968196	34.0	31.0	34.0	31.0	34.0
2	32.90415078304712	34.0	31.0	34.0	31.0	34.0
3	32.96825625436672	34.0	31.0	34.0	31.0	34.0
4	36.360885029152314	37.0	37.0	37.0	35.0	37.0
5	36.32247527058333	37.0	37.0	37.0	35.0	37.0
6	36.34811786769303	37.0	37.0	37.0	35.0	37.0
7	36.29688783202383	37.0	37.0	37.0	35.0	37.0
8	36.284611990243064	37.0	37.0	37.0	35.0	37.0
9	38.138701281721275	39.0	39.0	39.0	37.0	39.0
10-11	38.10010561840907	39.0	39.0	39.0	36.0	39.0
12-13	38.045692730103085	39.0	39.0	39.0	35.0	39.0
14-15	39.54625912669715	41.0	39.0	41.0	37.0	41.0
16-17	39.5144117034186	41.0	39.0	41.0	37.0	41.0
18-19	39.51011904032295	41.0	39.5	41.0	36.5	41.0
20-21	39.49593409983289	41.0	39.0	41.0	36.5	41.0
22-23	39.40945366477493	41.0	39.0	41.0	36.0	41.0
24-25	39.34173888350922	41.0	39.0	41.0	36.0	41.0
26-27	39.204013601689894	41.0	39.0	41.0	36.0	41.0
28-29	39.10239112315065	40.5	39.0	41.0	36.0	41.0
30-31	38.951653020032765	40.0	38.5	41.0	35.0	41.0
32-33	38.776825952302154	40.0	38.0	41.0	35.0	41.0
34-35	38.64622429509416	40.0	38.0	41.0	35.0	41.0
36-37	38.56317032960298	40.0	38.0	41.0	34.5	41.0
38-39	38.635801372017866	40.0	38.0	41.0	35.0	41.0
40-41	38.58485887582789	40.0	38.0	41.0	35.0	41.0
42-43	38.514488312516086	40.0	38.0	41.0	35.0	41.0
44-45	38.36900252912167	40.0	38.0	41.0	34.0	41.0
46-47	38.2105871729813	40.0	38.0	41.0	34.0	41.0
48-49	38.01605011665012	40.0	38.0	41.0	33.5	41.0
50-51	37.866935615671565	40.0	38.0	41.0	33.0	41.0
52-53	37.94527607875824	40.0	38.0	41.0	33.5	41.0
54-55	37.85121593959526	40.0	37.0	41.0	33.5	41.0
56-57	37.65013953070289	40.0	37.0	41.0	33.0	41.0
58-59	37.435776039942965	40.0	36.5	41.0	33.0	41.0
60-61	37.13582946202027	39.5	36.0	41.0	32.5	41.0
62-63	36.83541301496635	39.0	35.5	41.0	32.0	41.0
64-65	36.47004890724783	39.0	35.0	41.0	31.5	41.0
66-67	36.06741447360357	38.5	35.0	40.5	31.0	41.0
68-69	35.643799361795146	37.5	35.0	40.0	31.0	41.0
70-71	35.28786787688611	37.0	35.0	39.5	30.0	41.0
72-73	34.92111254795729	36.5	35.0	39.0	30.0	41.0
74-75	34.539204450273544	36.0	35.0	39.0	30.0	40.5
76-77	33.602753228818095	35.0	33.5	37.0	28.5	39.0
78-79	33.808720362493816	35.0	34.0	37.0	29.0	39.0
80-81	33.58045487417722	35.0	34.0	37.0	29.0	39.0
82-83	33.29847772616027	35.0	34.0	36.0	29.0	38.0
84-85	32.9997686405256	35.0	34.0	36.0	29.0	37.0
86-87	32.748962202092756	35.0	34.0	36.0	29.0	37.0
88-89	32.51879731888587	35.0	34.0	35.0	29.0	36.5
90-91	32.32165146742173	35.0	34.0	35.0	28.0	36.0
92-93	32.15170746356471	35.0	34.0	35.0	27.0	36.0
94-95	31.96987883505142	35.0	34.0	35.0	27.0	36.0
96-97	31.801939435911894	35.0	33.5	35.0	26.5	35.5
98-99	31.598566286276963	35.0	33.0	35.0	25.5	35.0
100-101	30.887439785249377	34.5	32.0	35.0	22.5	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22394232413129345
1101	2	0.07406444240580612
1101	3	0.22928653093313045
1101	4	0.15272573759152408
1101	5	0.1264404378023869
1101	6	0.15970157524928652
1101	7	0.15058276226470468
1101	8	0.05368894764786347
1101	9	-0.017293298329818185
1101	10-11	0.09102345197269557
1101	12-13	0.049715916241488856
1101	14-15	0.1576456436305449
1101	16-17	0.07806507730071388
1101	18-19	0.12295032053832955
1101	20-21	0.09742271654516088
1101	22-23	0.004709715871733522
1101	24-25	0.05486293976943557
1101	26-27	0.121169433420242
1101	28-29	0.21647953019273558
1101	30-31	0.16892719162762404
1101	32-33	0.15228494017001992
1101	34-35	0.025286193685595038
1101	36-37	0.21108457155995808
1101	38-39	0.12604036615835668
1101	40-41	-0.06932249904028964
1101	42-43	0.10986538866663409
1101	44-45	0.00949581628733398
1101	46-47	0.17329740538512795
1101	48-49	-0.1027987893339315
1101	50-51	0.0030638771956006394
1101	52-53	0.1389681841931676
1101	54-55	0.19571994705279394
1101	56-57	0.19149634742262123
1101	58-59	0.38472660476748644
1101	60-61	0.27417579298197126
1101	62-63	0.3965491576494813
1101	64-65	0.48718144933624785
1101	66-67	0.28823381235153533
1101	68-69	0.10981106530832818
1101	70-71	0.323660403830516
1101	72-73	0.23872415758126664
1101	74-75	0.22763568989828542
1101	76-77	-0.02772488837772613
1101	78-79	-0.1099715867670028
1101	80-81	-0.03468198966480429
1101	82-83	0.02727818183208086
1101	84-85	0.05114336869343106
1101	86-87	0.1138455454595757
1101	88-89	-0.11782722700259285
1101	90-91	-0.13406324616108378
1101	92-93	-0.3050255053426909
1101	94-95	-0.16986156550923326
1101	96-97	-0.010472060823406792
1101	98-99	-0.23725484008547326
1101	100-101	-0.36395077569437717
1102	1	0.14897470281325553
1102	2	0.13698611602561073
1102	3	0.15947485859152977
1102	4	0.16866328716055534
1102	5	0.1437285749617203
1102	6	0.1670812967233246
1102	7	0.1228087061745029
1102	8	0.06332940182721103
1102	9	0.03268306253733044
1102	10-11	0.27109127395385
1102	12-13	0.16508566461947538
1102	14-15	0.18393436137314012
1102	16-17	0.13925963121410234
1102	18-19	0.2746311629846545
1102	20-21	0.253659674596868
1102	22-23	0.16288785483287427
1102	24-25	0.14932322063621228
1102	26-27	0.17835996483102434
1102	28-29	0.30861362798636804
1102	30-31	0.08749846399816619
1102	32-33	0.26225849822141356
1102	34-35	0.12909278707864047
1102	36-37	0.11333791222373435
1102	38-39	-0.008430879570042293
1102	40-41	0.11124120572772966
1102	42-43	0.16489066790870055
1102	44-45	0.20336596583756972
1102	46-47	0.1829540296553489
1102	48-49	0.242127729153097
1102	50-51	0.12295124096257837
1102	52-53	0.21498455140514494
1102	54-55	0.2484714035159854
1102	56-57	0.26242991606928
1102	58-59	0.176341103640965
1102	60-61	0.44494966273111913
1102	62-63	0.4830730039135034
1102	64-65	0.3685445725969956
1102	66-67	0.5304918038414499
1102	68-69	0.5330762840588577
1102	70-71	0.48653864920625267
1102	72-73	0.41458621592603606
1102	74-75	0.5180713121464819
1102	76-77	0.5024854614044472
1102	78-79	0.4493595566740254
1102	80-81	0.41613475392529864
1102	82-83	0.5106899786063792
1102	84-85	0.49504162340161884
1102	86-87	0.4786474156460869
1102	88-89	0.3290547944743665
1102	90-91	0.22337815265957062
1102	92-93	0.3149504089219519
1102	94-95	0.19250401098530645
1102	96-97	0.11019993735321876
1102	98-99	0.28591921015268795
1102	100-101	0.2658627138574339
1103	1	0.1574315106938755
1103	2	0.10659656474163626
1103	3	0.12074013100836822
1103	4	0.05430531902707969
1103	5	-0.03944024490640885
1103	6	0.043523741340464994
1103	7	0.0657950227187385
1103	8	0.061589094096156316
1103	9	0.08801511640100301
1103	10-11	0.1471015039864696
1103	12-13	0.04247411533054901
1103	14-15	0.07416115953608227
1103	16-17	0.2043308249707465
1103	18-19	0.17595385410722741
1103	20-21	0.1100320489290354
1103	22-23	0.08299685305392046
1103	24-25	0.05952062248271517
1103	26-27	0.04291364602554637
1103	28-29	0.08457977785605664
1103	30-31	0.020830795509617417
1103	32-33	0.009954665680240282
1103	34-35	0.017074788632896798
1103	36-37	0.05935831790434776
1103	38-39	-0.017595403053462633
1103	40-41	-0.0031811652411875
1103	42-43	-0.09414111417500237
1103	44-45	3.9542083215593493E-4
1103	46-47	-0.010938577900958535
1103	48-49	0.07836491477781493
1103	50-51	-0.0957708064178675
1103	52-53	0.056126370742312304
1103	54-55	0.07138221131359046
1103	56-57	-0.010100700323107503
1103	58-59	0.06943955267757218
1103	60-61	-0.2315813715641042
1103	62-63	-0.3855631014609884
1103	64-65	-0.16709309418845208
1103	66-67	-0.23374014941068566
1103	68-69	-0.16172511906425058
1103	70-71	-0.28248773735101196
1103	72-73	-0.2659156109677383
1103	74-75	-0.15455404797270944
1103	76-77	0.004901283320585037
1103	78-79	0.06313074341791491
1103	80-81	-0.06162265347415996
1103	82-83	-0.1575803186628022
1103	84-85	-0.22413911528459352
1103	86-87	-0.17920107167443433
1103	88-89	0.014717435248236654
1103	90-91	-0.040338577909018625
1103	92-93	-0.05371217317377841
1103	94-95	-0.11392682680971333
1103	96-97	-0.12868091310768648
1103	98-99	0.07274081572968427
1103	100-101	-0.06130440577584295
1104	1	0.12796651241352208
1104	2	0.12635188401771558
1104	3	0.029187365834673074
1104	4	0.0709373936343809
1104	5	0.1259217500395664
1104	6	0.10934027993019413
1104	7	0.21179077606208097
1104	8	0.13623889099229558
1104	9	0.04918303993462558
1104	10-11	0.07968948135356868
1104	12-13	0.11603436195002104
1104	14-15	0.24708427486640971
1104	16-17	0.19201777812074994
1104	18-19	0.14230394699454507
1104	20-21	0.12760162810103992
1104	22-23	0.16067826881860725
1104	24-25	0.10724040157158043
1104	26-27	0.1732168211915024
1104	28-29	0.090674885436691
1104	30-31	0.09125119858346409
1104	32-33	0.13632921867248626
1104	34-35	0.08462093019077344
1104	36-37	0.04022910826319048
1104	38-39	0.10595800482875006
1104	40-41	0.11899188004188233
1104	42-43	0.06980997623841745
1104	44-45	0.08756475460635471
1104	46-47	0.13996778717075387
1104	48-49	0.05830948780822354
1104	50-51	0.24559970308797574
1104	52-53	0.14089218714811835
1104	54-55	0.22564951697061986
1104	56-57	0.080085645308003
1104	58-59	0.14054183761256667
1104	60-61	0.2627889533622323
1104	62-63	0.10755434024044064
1104	64-65	0.20024137849178913
1104	66-67	0.23488398960557788
1104	68-69	0.15059735594780221
1104	70-71	0.2523951343343569
1104	72-73	0.22575839240250417
1104	74-75	0.1485122293507004
1104	76-77	0.2694296162754597
1104	78-79	0.20219414183212336
1104	80-81	0.35523689214154075
1104	82-83	0.40605313564314827
1104	84-85	0.41055612269682484
1104	86-87	0.23201419185598127
1104	88-89	0.21428440374887003
1104	90-91	0.17414945297286266
1104	92-93	0.14047941504659178
1104	94-95	0.17280814755497786
1104	96-97	0.12052732046639747
1104	98-99	0.2725959407325078
1104	100-101	0.21682305500765153
1105	1	0.08507451244832254
1105	2	-0.017167952304816936
1105	3	0.03207235531272801
1105	4	0.04239949039153146
1105	5	0.02419386383816402
1105	6	0.03598000676979751
1105	7	0.07226219403254674
1105	8	0.0758766079103168
1105	9	-0.0612843383047732
1105	10-11	0.05535662740165748
1105	12-13	0.17754579314594565
1105	14-15	0.1330656622716475
1105	16-17	0.09310651671783177
1105	18-19	0.18064969576801815
1105	20-21	0.07776501968522354
1105	22-23	0.0828053053091864
1105	24-25	0.11525647100026504
1105	26-27	0.16290369852910658
1105	28-29	0.24872905263681133
1105	30-31	0.30658626630799546
1105	32-33	0.03457212210799554
1105	34-35	0.1906365247956856
1105	36-37	0.2596101220243767
1105	38-39	0.1830466390143215
1105	40-41	0.07116208572012539
1105	42-43	0.022107588819508805
1105	44-45	0.08098247895747335
1105	46-47	0.1666430636437326
1105	48-49	0.15575215159445577
1105	50-51	0.12598823820691507
1105	52-53	0.19089287079490447
1105	54-55	0.3467967011537709
1105	56-57	0.23720756687755795
1105	58-59	0.21091022379594193
1105	60-61	0.2742903784550492
1105	62-63	0.24990141155971912
1105	64-65	0.3719566731622024
1105	66-67	0.5246837308141892
1105	68-69	0.46813451215115265
1105	70-71	0.37612028843455647
1105	72-73	0.3809729394119188
1105	74-75	0.5740059155617914
1105	76-77	0.4933804981176806
1105	78-79	0.5409423721191686
1105	80-81	0.5815258460252224
1105	82-83	0.7371184189401632
1105	84-85	0.8264768892761936
1105	86-87	0.685749327199396
1105	88-89	0.6505591999289067
1105	90-91	0.6835502179114741
1105	92-93	0.5890772554685171
1105	94-95	0.482410650448049
1105	96-97	0.7099358914084242
1105	98-99	0.6228698344987684
1105	100-101	0.6442763215341927
1106	1	0.006971617362658833
1106	2	0.01941270004953566
1106	3	0.05608905439476786
1106	4	0.033485054604952325
1106	5	0.053573346761247365
1106	6	0.0369462741285389
1106	7	0.03360778326981517
1106	8	0.011168903786334283
1106	9	-0.0593889677164654
1106	10-11	-0.03504140042188908
1106	12-13	0.044622710505308305
1106	14-15	0.10330648582875313
1106	16-17	0.058077466756849105
1106	18-19	0.13044095724244187
1106	20-21	0.09223053756736022
1106	22-23	0.02829667703719707
1106	24-25	0.05897139735335344
1106	26-27	0.08978637137327183
1106	28-29	0.1005661994227367
1106	30-31	0.12355045247551288
1106	32-33	0.03035275464147702
1106	34-35	0.16012477473684328
1106	36-37	-0.021516074970278964
1106	38-39	0.11456801458261623
1106	40-41	0.22434890979751998
1106	42-43	0.018304459599825407
1106	44-45	0.07697990224452411
1106	46-47	0.05671404713046968
1106	48-49	0.05177249276115958
1106	50-51	0.26475909657442287
1106	52-53	0.31853555008085976
1106	54-55	0.2112473533842376
1106	56-57	0.43365467612770914
1106	58-59	0.30983803866902093
1106	60-61	0.22033363750141888
1106	62-63	0.23462323805277663
1106	64-65	0.3709569580871914
1106	66-67	0.2523022534161754
1106	68-69	0.30792986167978853
1106	70-71	0.1549039064864175
1106	72-73	0.13243472849310223
1106	74-75	0.11852730351814245
1106	76-77	0.16015266961774444
1106	78-79	0.17790953141701493
1106	80-81	0.25873308559758357
1106	82-83	0.18222328372467445
1106	84-85	0.25795614549808477
1106	86-87	0.36620595046662174
1106	88-89	0.21732393860313692
1106	90-91	0.3723842552985914
1106	92-93	0.17147012986614385
1106	94-95	0.2615962604972317
1106	96-97	0.3802621924626486
1106	98-99	0.3083597196616097
1106	100-101	0.4945253211997134
1107	1	0.22927240135905436
1107	2	0.173984685775757
1107	3	0.18947854539406705
1107	4	0.12379188771055993
1107	5	0.161971792034052
1107	6	0.12359711052401678
1107	7	0.17177288680774438
1107	8	0.17224500847837731
1107	9	0.18777551233871748
1107	10-11	0.26462430670040504
1107	12-13	0.16434400045131525
1107	14-15	0.2899765573302773
1107	16-17	0.24800954693408528
1107	18-19	0.21956582624473953
1107	20-21	0.20718085793136964
1107	22-23	0.2383188305731423
1107	24-25	0.18747517141381564
1107	26-27	0.21183311931379478
1107	28-29	0.25984813301518983
1107	30-31	0.2947720806940808
1107	32-33	0.23737688475038965
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2316	92-93	-0.06754017115059696
2316	94-95	-0.05581195694270846
2316	96-97	-0.31922192193373533
2316	98-99	-0.2564131404148533
2316	100-101	-0.03152940608211807
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	2.0
5	11.0
6	22.0
7	55.0
8	143.0
9	284.0
10	455.0
11	781.0
12	1074.0
13	1416.0
14	1756.0
15	2115.0
16	2568.0
17	3477.0
18	3624.0
19	2766.0
20	2570.0
21	3081.0
22	3834.0
23	4179.0
24	4979.0
25	5870.0
26	5304.0
27	5162.0
28	6930.0
29	8530.0
30	10844.0
31	14218.0
32	18391.0
33	24505.0
34	34905.0
35	55599.0
36	99570.0
37	210397.0
38	329002.0
39	110465.0
40	109.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.854813498023105	20.567832367875276	14.347728364033877	24.229625770067738
2	15.76094284348455	21.767504668047675	39.26686115162881	23.204691336838966
3	18.107464583143937	25.878794626668693	31.52925025255801	24.484490537629355
4	13.373919493105497	19.23003443932151	36.986155821839084	30.409890245733905
5	13.684734156217186	37.029364777792765	35.322309794932764	13.963591271057288
6	29.67979440161145	37.25428908800444	18.671169238689433	14.394747271694674
7	25.418183526796838	32.591042251449444	23.458318522241154	18.53245569951256
8	24.032069589661244	34.665208029450575	22.36076551896024	18.941956861927935
9	24.775177835251625	16.415797408773887	21.73747390183412	37.07155085414036
10-11	23.44447781196246	26.321047277006237	29.859876853429434	20.37459805760187
12-13	24.286258575111646	24.58952845568317	28.922845445742375	22.20136752346281
14-15	22.067721455308018	26.56038766266035	26.296392577902555	25.07549830412908
16-17	21.8132425450545	28.982176365016276	27.316158331979885	21.88842275794934
18-19	21.653317099006074	27.89762567856543	28.712951183125966	21.73610603930253
20-21	22.33548451679067	27.16538167673821	28.533364794136034	21.965769012335087
22-23	22.179406248850864	27.539029781531283	28.07263768187	22.208926287747857
24-25	22.11347135228336	27.67830512075637	28.398073127980094	21.81015039898018
26-27	22.052473226356227	27.72747957215286	28.51803559672705	21.702011604763868
28-29	21.625518543980597	27.178855152955787	28.91358332405462	22.282042979008992
30-31	22.311259154162766	27.0747690615218	28.720283923964846	21.893687860350592
32-33	21.98555661673451	27.616075669436512	28.29376043800019	22.104607275828783
34-35	22.073350877121293	27.56000711013767	28.69834423690652	21.66829777583452
36-37	22.4263804558344	27.37488661343968	28.207000956124507	21.99173197460141
38-39	22.074860367151654	27.144339711296062	28.5276446481906	22.25315527336169
40-41	22.114748170574753	27.052459867047464	28.734846720517755	22.097945241860028
42-43	22.34952951799599	26.96670875059755	28.563242342154616	22.120519389251847
44-45	22.215616815594753	27.30123514294236	28.289032845895186	22.194115195567704
46-47	22.52430040572178	26.93361362048466	28.04648844326228	22.495597530531278
48-49	22.268987820174953	27.04306248442282	28.261351425337793	22.42659827006443
50-51	22.153359155706458	27.3150247804894	28.174221345133176	22.357394718670964
52-53	22.137628754356502	27.392502114411087	28.40859411070117	22.061275020531237
54-55	22.075677530858144	27.433666736125584	28.108490739492296	22.38216499352398
56-57	22.31204213296071	27.80251400414302	27.604249659855608	22.281194203040666
58-59	21.949390853662894	27.934162961328628	28.226963829159146	21.889482355849328
60-61	22.212790646600904	28.221078856484443	27.684806957438397	21.881323539476252
62-63	21.917697667695368	28.542562439792295	27.489546972478372	22.050192920033957
64-65	22.039366664436443	28.385337528875848	27.470146086393378	22.105149720294328
66-67	21.813354825430338	28.369568048688183	27.49920962404663	22.317867501834847
68-69	21.961030669833246	28.21302893332312	27.627109987486914	22.198830409356727
70-71	21.947030291825225	27.885476256992515	27.65697627205901	22.51051717912325
72-73	22.123895613303002	27.83274102419257	27.511780635526083	22.531582726978343
74-75	22.047225594635826	27.838032891715986	27.48954353154613	22.625197982102062
76-77	22.090622800646795	27.802939995771116	27.48291335582611	22.623523847755983
78-79	22.145124781407816	27.783373102129538	27.49261485282822	22.57888726363443
80-81	21.996349026526254	27.661078518403503	27.596364145274826	22.746208309795417
82-83	22.070192088156137	27.76577166501771	27.516665398235467	22.647370848590686
84-85	22.276837084444825	27.550252738446734	27.490036788421378	22.682873388687064
86-87	22.130051553605227	27.492004441352798	27.496549984116136	22.881394020925843
88-89	22.157297264997165	27.42965096295328	27.504676997344724	22.908374774704836
90-91	22.10953875194587	27.37258374906537	27.594545840841022	22.923331658147735
92-93	22.045238182791348	27.38417725915121	27.55378980097978	23.016794757077662
94-95	22.023736562764302	27.277792759112394	27.574627475495305	23.123843202628
96-97	22.018742680635405	27.23413948276575	27.633017720714754	23.11410011588409
98-99	22.019803962426813	27.18269533276949	27.46737473901834	23.330125965785356
100-101	21.97362688772454	27.064233068893994	27.561071901179734	23.40106814220173
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	705.0
1	429.5
2	168.0
3	156.0
4	106.5
5	111.0
6	184.5
7	322.0
8	460.0
9	504.0
10	405.0
11	275.5
12	251.5
13	248.5
14	262.0
15	296.0
16	328.0
17	385.5
18	434.5
19	457.5
20	493.0
21	580.0
22	653.5
23	734.0
24	930.0
25	1239.0
26	1675.5
27	2321.5
28	3513.0
29	5484.0
30	8518.0
31	12854.0
32	17874.5
33	22929.5
34	27412.5
35	30852.0
36	34256.5
37	37201.0
38	38069.0
39	38069.5
40	37420.0
41	36694.0
42	36221.5
43	35856.0
44	36559.5
45	37001.5
46	37147.0
47	37274.5
48	37019.5
49	36310.5
50	34777.5
51	33025.0
52	31146.0
53	29653.0
54	27698.5
55	25140.0
56	22764.0
57	20268.0
58	18325.5
59	16241.0
60	13658.0
61	11213.5
62	9054.5
63	6900.0
64	4978.5
65	3719.5
66	2780.0
67	2091.0
68	1596.5
69	1147.5
70	894.0
71	715.5
72	509.5
73	368.5
74	308.0
75	233.0
76	164.0
77	109.0
78	65.5
79	51.0
80	43.0
81	23.0
82	10.0
83	7.0
84	6.0
85	3.5
86	1.5
87	1.5
88	1.5
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.018999056175919002
2	0.0
3	0.0013278910230481024
4	0.0176711651528709
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	5.107273165569624E-5
16-17	0.0015321819496708874
18-19	5.107273165569624E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.5321819496708875E-4
28-29	0.005413709555503802
30-31	1.5321819496708875E-4
32-33	1.0214546331139248E-4
34-35	0.011440291890875958
36-37	0.0044944003857012695
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	3.064363899341775E-4
60-61	0.0015321819496708874
62-63	0.009499528087959501
64-65	0.0020939819978835457
66-67	0.003217582094308863
68-69	0.0021450547295392424
70-71	2.5536365827848125E-4
72-73	0.006026582335372156
74-75	0.005771218677093675
76-77	0.0015321819496708874
78-79	4.0858185324556994E-4
80-81	0.008069491601600007
82-83	0.009857037209549375
84-85	0.0019918365345721533
86-87	0.0015321819496708874
88-89	2.5536365827848125E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	5.107273165569624E-5
98-99	0.0
100-101	6.639455115240512E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	978996.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.72723219217083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.47439136298743	37.79655392852241
2	20.146934310329577	23.26053509785617
3	7.453108946175712	12.90742052068293
4	3.1507098966257785	7.275270470907475
5	1.4589956837678395	4.211189130212055
6	0.7606760723413623	2.634703455064701
7	0.4581372992040894	1.851289877293396
8	0.2861558299307719	1.3215187230045615
9	0.1872029105056187	0.9726035293627225
>10	0.6107322974692386	5.395102631956763
>50	0.008697943181923655	0.3329950211879227
>100	0.002483511526995292	0.31786337049691754
>500	7.095743814336874E-4	0.2453609727970844
>1k	8.869679767921091E-4	0.9176459420845882
>5k	1.7739359535842185E-4	0.5599473285703078
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	5468	0.5585313933866941	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	2737	0.2795721330832812	TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT	1768	0.1805931791345419	RNA PCR Primer, Index 11 (95% over 23bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG	1633	0.16680354158750393	TruSeq Adapter, Index 11 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1441	0.14719161263171657	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1382	0.14116503029634442	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.0214546331139248E-4	0.0
3	0.0	0.0	0.0	1.0214546331139248E-4	0.0
4	0.0	0.0	0.0	1.0214546331139248E-4	0.0
5	0.0	0.0	0.0	1.0214546331139248E-4	0.0
6	0.0	0.0	0.0	1.0214546331139248E-4	0.0
7	0.0	0.0	0.0	1.0214546331139248E-4	0.0
8	0.0	0.0	0.0	1.0214546331139248E-4	0.0
9	0.0	0.0	0.0	1.0214546331139248E-4	0.0
10-11	0.0	0.0	0.0	1.0214546331139248E-4	0.0
12-13	0.0	0.0	0.0	1.0214546331139248E-4	0.0
14-15	0.0	0.0	0.0	1.0214546331139248E-4	0.0
16-17	0.0	0.0	0.0	1.0214546331139248E-4	0.0
18-19	0.0	0.0	0.0	2.0429092662278497E-4	0.0
20-21	0.0	0.0	0.0	2.0429092662278497E-4	1.0214546331139248E-4
22-23	0.0	0.0	0.0	3.575091215898737E-4	1.0214546331139248E-4
24-25	0.0	0.0	0.0	6.128727798683549E-4	1.0214546331139248E-4
26-27	0.0	0.0	0.0	0.0014300364863594948	2.0429092662278497E-4
28-29	0.0	0.0	0.0	0.0032175820943088638	2.0429092662278497E-4
30-31	0.0	0.0	0.0	0.007558764285043044	2.0429092662278497E-4
32-33	0.0	0.0	0.0	0.017620092421215205	3.0643638993417744E-4
34-35	0.0	0.0	0.0	0.034014439282693695	3.0643638993417744E-4
36-37	0.0	0.0	0.0	0.05955080511054182	3.0643638993417744E-4
38-39	0.0	0.0	0.0	0.08697686200965071	3.0643638993417744E-4
40-41	0.0	0.0	0.0	0.1182844465145925	3.0643638993417744E-4
42-43	0.0	0.0	0.0	0.15454608599013683	3.0643638993417744E-4
44-45	0.0	0.0	0.0	0.20117548999178753	4.596545849012662E-4
46-47	0.0	0.0	0.0	0.25255465803741795	6.128727798683549E-4
48-49	0.0	0.0	0.0	0.30245271686503317	6.128727798683549E-4
50-51	0.0	0.0	0.0	0.3518911211077471	8.171637064911399E-4
52-53	5.107273165569624E-5	0.0	0.0	0.4009209434972155	8.171637064911399E-4
54-55	1.0214546331139248E-4	0.0	0.0	0.4610846213876257	8.171637064911399E-4
56-57	1.0214546331139248E-4	0.0	0.0	0.5356508096049422	8.171637064911399E-4
58-59	1.0214546331139248E-4	0.0	0.0	0.6084294522143093	8.171637064911399E-4
60-61	1.0214546331139248E-4	0.0	0.0	0.6815145312136106	8.171637064911399E-4
62-63	1.0214546331139248E-4	0.0	0.0	0.7601154652317272	8.171637064911399E-4
64-65	1.0214546331139248E-4	0.0	0.0	0.8442322542686589	8.171637064911399E-4
66-67	1.0214546331139248E-4	0.0	0.0	0.952659663573702	8.171637064911399E-4
68-69	1.0214546331139248E-4	0.0	0.0	1.0780432197884364	8.171637064911399E-4
70-71	1.0214546331139248E-4	0.0	0.0	1.2002091939088617	8.682364381468362E-4
72-73	1.0214546331139248E-4	0.0	0.0	1.337186260209439	9.193091698025324E-4
74-75	1.0214546331139248E-4	0.0	0.0	1.4813645816734695	9.703819014582287E-4
76-77	1.0214546331139248E-4	0.0	0.0	1.651283559891971	0.001021454633113925
78-79	1.0214546331139248E-4	0.0	0.0	1.8324385390747255	0.001021454633113925
80-81	1.0214546331139248E-4	0.0	0.0	2.0312646834103507	0.001021454633113925
82-83	1.0214546331139248E-4	0.0	0.0	2.228967227649551	0.0011236000964253174
84-85	1.0214546331139248E-4	0.0	0.0	2.464872175167212	0.0011236000964253174
86-87	1.0214546331139248E-4	0.0	0.0	2.7461297083951313	0.0011236000964253174
88-89	1.0214546331139248E-4	0.0	0.0	3.0629849355870706	0.0011236000964253174
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1250	0.0	49.03835	1
GTATCAA	2095	0.0	37.424503	1
ATCAACG	2440	0.0	32.514107	3
TCAACGC	2455	0.0	32.508953	4
CAACGCA	2495	0.0	31.981236	5
TATCAAC	2570	0.0	30.869427	2
AACGCAG	2590	0.0	30.808176	6
ACGCAGA	2975	0.0	26.501936	7
CGCAGAG	3030	0.0	26.02088	8
GCAGAGT	3480	0.0	21.837214	9
GTACATG	2340	0.0	21.119017	1
TACATGG	2350	0.0	20.41732	2
GTGGTAT	725	0.0	20.31795	1
ACATGGG	2395	0.0	19.636988	3
GAGTACT	1855	0.0	19.07519	12-13
AGAGTAC	2995	0.0	18.633633	10-11
CAGAGTA	3445	0.0	18.336603	10-11
GGCCCGT	135	0.009404061	17.59109	6
CATGGGG	1610	0.0	17.408901	4
TGGTATC	840	0.0	16.966303	2
>>END_MODULE