FastQCFastQC Report
Fri 10 Feb 2017
SRR2031715_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031715_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459996
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15200.052.812691
GTATCAA29100.036.5637051
TCAACGC30150.034.187524
CAACGCA30500.033.949785
ATCAACG30800.033.466033
AACGCAG32650.031.7109266
TATCAAC33300.031.0962092
ACGCAGA36950.027.5074187
CGCAGAG37100.026.886898
GTACATG32900.022.5228771
GCAGAGT44400.021.5034589
TACATGG34300.020.0802482
TACATCG1200.00476494819.791065
GTGGTAT9250.019.5135841
TAGTACG1250.00603177819.0000694
CAGAGTA41700.018.8542510-11
ACATGGG33300.018.6862553
GAGTACT19250.018.63377212-13
AGAGTAC36300.017.53660410-11
CATGGGG19750.016.8355034
Produced by FastQC (version 0.11.5)