FastQCFastQC Report
Fri 10 Feb 2017
SRR2031715_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031715_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459996
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT43320.2967131416798402No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT22990.15746618483886257TruSeq Adapter, Index 11 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18900.12945240945865605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17040.11671264852780418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18700.042.6823351
GTATCAA32200.035.2632681
TCAACGC33600.033.6468054
ATCAACG34350.032.912163
CAACGCA34600.032.6709985
AACGCAG36350.031.0981166
TATCAAC37200.031.029122
ACGCAGA41000.027.4552867
CGCAGAG41800.026.8161988
CGGAGAT2850.026.6720051
TAGGTCG1201.7119829E-423.7482435
GTACATG31450.021.9042241
GAGTACT25750.021.76687212-13
GCAGAGT49800.021.5546329
ACATGGG31300.019.7290043
AGAGTAC40050.019.21337910-11
TACATGG33450.018.7449362
CAGAGTA47850.018.7120210-11
GTGGTAT12250.017.452471
GTACTTT31800.016.95352414-15
Produced by FastQC (version 0.11.5)