FastQCFastQC Report
Fri 10 Feb 2017
SRR2031714_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031714_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4431311
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49700.11215642503990356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47250.051.9860841
GTATCAA85100.038.2994841
TCAACGC97450.033.3954624
ATCAACG98300.032.96173
CAACGCA99900.032.5749865
TATCAAC100900.032.2550542
AACGCAG104000.031.3739816
ACGCAGA119250.026.9638217
CGCAGAG122850.025.6347318
GTGGTAT26650.021.036971
GTACATG94350.020.495141
GCAGAGT146400.020.4728689
CAGAGTA134250.018.61234710-11
TACATGG98600.018.5998212
GAGTACT74500.018.39779712-13
AGAGTAC118100.017.93967810-11
TGGTATC29150.017.9288542
ACATGGG101850.016.9792733
ACTATCG1700.001786306316.7679923
GTACTTT81600.016.39010414-15
Produced by FastQC (version 0.11.5)