##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031714_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4431311
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.947001237331346	33.0	31.0	34.0	30.0	34.0
2	32.260815365926696	34.0	31.0	34.0	31.0	34.0
3	32.37871568932986	34.0	31.0	34.0	31.0	34.0
4	35.82065284968715	37.0	35.0	37.0	35.0	37.0
5	35.8188472892108	37.0	37.0	37.0	35.0	37.0
6	35.84072478776597	37.0	37.0	37.0	35.0	37.0
7	35.820006765492195	37.0	37.0	37.0	35.0	37.0
8	35.806282610270415	37.0	37.0	37.0	35.0	37.0
9	37.58015607570762	39.0	38.0	39.0	35.0	39.0
10-11	37.50388756284539	39.0	38.0	39.0	35.0	39.0
12-13	37.45247535097401	39.0	38.0	39.0	35.0	39.0
14-15	38.85620237442147	41.0	39.0	41.0	36.0	41.0
16-17	38.7808929456768	41.0	39.0	41.0	35.0	41.0
18-19	38.7721130383311	41.0	39.0	41.0	35.0	41.0
20-21	38.76323338623716	41.0	39.0	41.0	35.0	41.0
22-23	38.6590502223834	40.5	39.0	41.0	35.0	41.0
24-25	38.588385355936424	40.5	38.5	41.0	34.0	41.0
26-27	38.477054871571866	40.0	38.0	41.0	34.0	41.0
28-29	38.36825840027929	40.0	38.0	41.0	34.0	41.0
30-31	38.22414517960938	40.0	38.0	41.0	34.0	41.0
32-33	38.04454674925773	40.0	38.0	41.0	33.5	41.0
34-35	37.89102581606211	40.0	38.0	41.0	33.0	41.0
36-37	37.730451101265515	40.0	38.0	41.0	33.0	41.0
38-39	37.55215443014494	40.0	38.0	41.0	32.5	41.0
40-41	37.468651489367375	40.0	37.5	41.0	32.0	41.0
42-43	37.46452641216109	40.0	38.0	41.0	32.0	41.0
44-45	37.35576153422768	40.0	37.5	41.0	32.0	41.0
46-47	37.337448217920155	40.0	37.5	41.0	32.0	41.0
48-49	37.43508772009006	40.0	38.0	41.0	32.0	41.0
50-51	36.89108234560833	39.5	37.0	40.5	31.5	41.0
52-53	36.91174361266903	39.5	37.0	40.5	31.5	41.0
54-55	37.13826359738687	40.0	37.0	41.0	32.0	41.0
56-57	37.002631275484845	40.0	36.5	41.0	31.0	41.0
58-59	36.80287583065147	40.0	36.0	41.0	31.0	41.0
60-61	36.5464996701879	39.0	35.5	41.0	31.0	41.0
62-63	36.24677708244806	39.0	35.0	41.0	30.5	41.0
64-65	35.95375736435561	39.0	35.0	41.0	30.0	41.0
66-67	35.646694172446935	38.0	35.0	40.5	30.0	41.0
68-69	35.29230130767171	37.0	35.0	40.0	29.0	41.0
70-71	34.911557550350224	37.0	35.0	39.5	29.0	41.0
72-73	34.53222894985254	36.0	34.5	39.0	29.0	41.0
74-75	34.11402065889756	36.0	34.0	39.0	28.5	40.5
76-77	33.730244277596405	35.0	34.0	37.5	28.0	39.5
78-79	33.34135744478327	35.0	34.0	37.0	27.5	39.0
80-81	32.99329938702113	35.0	34.0	36.5	27.0	39.0
82-83	32.67391546203821	35.0	34.0	36.0	26.0	37.5
84-85	32.36241712666974	35.0	34.0	36.0	26.0	37.0
86-87	32.095066448732666	35.0	33.0	35.0	26.0	37.0
88-89	31.834762556724186	35.0	33.0	35.0	25.0	36.0
90-91	31.598691109696432	35.0	33.0	35.0	25.0	36.0
92-93	31.38385705720045	35.0	33.0	35.0	24.0	36.0
94-95	31.13411290699299	35.0	33.0	35.0	23.0	35.5
96-97	30.864302573211404	35.0	32.0	35.0	20.0	35.0
98-99	30.603955240334066	35.0	32.0	35.0	17.5	35.0
100-101	29.994936261526217	34.5	31.5	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.05777219665358757
1101	2	0.09367315466867865
1101	3	0.20663361122578294
1101	4	0.3157567111919235
1101	5	0.1315412524091144
1101	6	0.2554057907718388
1101	7	0.22193039717119234
1101	8	0.24531684967302425
1101	9	0.31285321940502087
1101	10-11	0.15341647969192707
1101	12-13	0.2289412950641605
1101	14-15	0.20572622067665236
1101	16-17	0.27165685506280823
1101	18-19	0.3029504496780149
1101	20-21	0.2957184960391359
1101	22-23	0.36173174858807755
1101	24-25	0.20422071120921714
1101	26-27	0.08216338676311352
1101	28-29	0.06524399768284894
1101	30-31	0.17666183946578684
1101	32-33	0.08567640860820092
1101	34-35	0.24310994211423065
1101	36-37	-0.09484271227388064
1101	38-39	0.1524049539244956
1101	40-41	-0.018255961187989556
1101	42-43	-0.08100904873647607
1101	44-45	-0.2600376544656413
1101	46-47	-0.22294499482260477
1101	48-49	-0.019362739600730094
1101	50-51	0.04178003219465154
1101	52-53	-0.010307659019041182
1101	54-55	0.06672840034339345
1101	56-57	-0.07388027457916024
1101	58-59	-0.28707316171641395
1101	60-61	-0.4275608661145256
1101	62-63	-0.20242492635041742
1101	64-65	-0.059044053631915006
1101	66-67	-0.37106548779034654
1101	68-69	-0.4338273320291748
1101	70-71	-0.48941207876047343
1101	72-73	-0.10045835966074179
1101	74-75	0.04787596765718405
1101	76-77	-0.19910771565309204
1101	78-79	-0.3541095715708167
1101	80-81	-0.43797772176007754
1101	82-83	-0.4109549466515787
1101	84-85	-0.5191293993175563
1101	86-87	-0.4800003275293818
1101	88-89	-0.705398208235934
1101	90-91	-1.096431040331467
1101	92-93	-0.9009194000890766
1101	94-95	-0.9310561728172253
1101	96-97	-1.0063221234464699
1101	98-99	-0.9931744393072819
1101	100-101	-0.9043789444935939
1102	1	0.02554058547712046
1102	2	0.13933642052377593
1102	3	0.19243642440925157
1102	4	0.3020845304905393
1102	5	0.18020097685801773
1102	6	0.1380898423930219
1102	7	0.04857551556118267
1102	8	-0.036629438880204646
1102	9	0.13741086642502154
1102	10-11	0.1867655581874743
1102	12-13	0.21904207127555253
1102	14-15	0.4337703989477575
1102	16-17	0.24362928273728102
1102	18-19	0.2356024012532103
1102	20-21	0.1440834739205954
1102	22-23	0.2665487639795927
1102	24-25	0.09234401699031025
1102	26-27	0.10239312266760692
1102	28-29	0.1616594088611265
1102	30-31	0.0013665728417322498
1102	32-33	0.05435103178236034
1102	34-35	0.18539452569952175
1102	36-37	0.12709108774749467
1102	38-39	-0.014383800514814027
1102	40-41	0.06187149213424448
1102	42-43	0.12163965338156402
1102	44-45	-0.07284833449166683
1102	46-47	-0.13939269972311052
1102	48-49	0.017995831900599057
1102	50-51	-0.07069264690029087
1102	52-53	-0.12209088651189148
1102	54-55	-0.06488558996849036
1102	56-57	-0.026417110726889348
1102	58-59	-0.12570992476929632
1102	60-61	-0.15884463841150875
1102	62-63	-0.10637889882973894
1102	64-65	-0.042886217526501014
1102	66-67	-0.07803621900428226
1102	68-69	-0.08556482323304238
1102	70-71	-0.09179813435704176
1102	72-73	-0.12675059274079103
1102	74-75	-0.40913037846810596
1102	76-77	0.005109333739369504
1102	78-79	-0.22902354703418837
1102	80-81	-0.22852836487284378
1102	82-83	-0.13857353928514016
1102	84-85	-0.15613960462155774
1102	86-87	-0.20245314404838766
1102	88-89	-0.2079351519815873
1102	90-91	-0.1527439895976137
1102	92-93	-0.09408277174878776
1102	94-95	-0.46337718905805403
1102	96-97	-0.6115458589736562
1102	98-99	-0.42669380944340674
1102	100-101	-0.5196360425490134
1103	1	0.003598274100454546
1103	2	0.17059648644860204
1103	3	0.18526647334851987
1103	4	0.17951113343935532
1103	5	0.17370157971340916
1103	6	0.1275140461060147
1103	7	0.18398015304572368
1103	8	0.22219706130223926
1103	9	0.13494562454156522
1103	10-11	0.09880604596890663
1103	12-13	0.17537509928852302
1103	14-15	0.3941347388837215
1103	16-17	0.3082085668524286
1103	18-19	0.3103981040214663
1103	20-21	0.10784790470798811
1103	22-23	0.1381383998766097
1103	24-25	0.1992652775098236
1103	26-27	0.17212663418347063
1103	28-29	0.046241136893122814
1103	30-31	0.21224399991857013
1103	32-33	-0.020215071429461773
1103	34-35	-0.028075484191234068
1103	36-37	-0.02163157643327196
1103	38-39	0.07220809989794219
1103	40-41	0.17326366496587298
1103	42-43	0.14518560748059883
1103	44-45	0.0010926066668019985
1103	46-47	-0.03163187591326988
1103	48-49	0.13269683796842457
1103	50-51	0.15368457741945463
1103	52-53	0.20591927774504626
1103	54-55	0.014967125114814905
1103	56-57	-0.007941302457247446
1103	58-59	0.11164192546531382
1103	60-61	0.1770060985208204
1103	62-63	0.23160853903147682
1103	64-65	0.2018166096317131
1103	66-67	0.07647494008251243
1103	68-69	0.043025246611527734
1103	70-71	0.1230676837762843
1103	72-73	0.05442038192168752
1103	74-75	-0.023409318065446882
1103	76-77	-0.0222981443753838
1103	78-79	0.008886071369524018
1103	80-81	0.04593307641526678
1103	82-83	0.04274132859779911
1103	84-85	-0.006756270509697515
1103	86-87	0.15554371067802464
1103	88-89	0.18026621322692193
1103	90-91	0.13454154992918888
1103	92-93	-0.3750658491478376
1103	94-95	0.16125048596463998
1103	96-97	0.06616047429130134
1103	98-99	0.039751923078355844
1103	100-101	0.009311847863315847
1104	1	0.04767695455664622
1104	2	0.1295099001477169
1104	3	0.23073268449716267
1104	4	0.12316376731369161
1104	5	0.1421569758136485
1104	6	0.19592901302025467
1104	7	0.18068504898992188
1104	8	0.09785348897371193
1104	9	0.14995805457100886
1104	10-11	0.2722661598792797
1104	12-13	0.2365213654213747
1104	14-15	0.3847795332771682
1104	16-17	0.4261577428577823
1104	18-19	0.36984543762758904
1104	20-21	0.2849570950844438
1104	22-23	0.4036614678235537
1104	24-25	0.35016031743966636
1104	26-27	0.3451182466867948
1104	28-29	0.30391073739270524
1104	30-31	0.3173015901268741
1104	32-33	0.36779447569741563
1104	34-35	0.366055287345155
1104	36-37	0.3221064686782853
1104	38-39	0.211158556428785
1104	40-41	0.31906480108580837
1104	42-43	0.1955272106306154
1104	44-45	0.22832520991476457
1104	46-47	0.22893593197279216
1104	48-49	0.36705776198656537
1104	50-51	0.3125612788197216
1104	52-53	0.3187184429502068
1104	54-55	0.3131415676315683
1104	56-57	0.3646056082918747
1104	58-59	0.2927990181573463
1104	60-61	0.3306292886041575
1104	62-63	0.31150489318316943
1104	64-65	0.23849961709757395
1104	66-67	0.30730890474006145
1104	68-69	0.3260859128333209
1104	70-71	0.3719526337079557
1104	72-73	0.2673405581690105
1104	74-75	0.2387932449533139
1104	76-77	0.25423015698725493
1104	78-79	0.1863658251058169
1104	80-81	0.14313980356945422
1104	82-83	0.14498996815601117
1104	84-85	0.28214949013165125
1104	86-87	0.22857687772245328
1104	88-89	0.3301276948745908
1104	90-91	0.35870405583649756
1104	92-93	0.15644972059019224
1104	94-95	0.29367703387611854
1104	96-97	0.09498676685649343
1104	98-99	0.14130178142186978
1104	100-101	0.12102536692139765
1105	1	-0.11198509770191478
1105	2	0.089227598983328
1105	3	0.08524008184940612
1105	4	0.09949237548721612
1105	5	0.188360001402458
1105	6	0.1929712657787661
1105	7	0.18928448789719
1105	8	0.23426248319320564
1105	9	0.19624315947624638
1105	10-11	0.22636234847023218
1105	12-13	0.2006216936648073
1105	14-15	0.3136140586679801
1105	16-17	0.32580716450009106
1105	18-19	0.23876049304158897
1105	20-21	0.31081174135211853
1105	22-23	0.055164411480724596
1105	24-25	0.1375406471499474
1105	26-27	0.16662840520201172
1105	28-29	0.012726702028949433
1105	30-31	0.1677644891683343
1105	32-33	0.2802521777768163
1105	34-35	0.189951622029902
1105	36-37	0.2793444061567314
1105	38-39	0.14830705821668744
1105	40-41	0.041159125602568736
1105	42-43	0.11308524390682351
1105	44-45	0.29683424343880915
1105	46-47	0.34815856947287926
1105	48-49	0.3261940451545442
1105	50-51	0.1826642385235786
1105	52-53	0.2964799627884176
1105	54-55	0.2913675677994263
1105	56-57	0.3745370209938059
1105	58-59	0.24230651764727185
1105	60-61	0.2531762553242629
1105	62-63	0.2482177100837859
1105	64-65	0.28177384470905764
1105	66-67	0.35873030438068554
1105	68-69	0.4266461195501421
1105	70-71	0.3394350727951334
1105	72-73	0.15590241164056806
1105	74-75	0.21320874054379146
1105	76-77	0.34147859372698974
1105	78-79	0.3525793942565869
1105	80-81	0.38158698596002694
1105	82-83	0.31478567802889046
1105	84-85	0.37611652501080073
1105	86-87	0.33081703392665673
1105	88-89	0.20985639258471878
1105	90-91	0.08959095792190652
1105	92-93	0.19607658703324304
1105	94-95	0.3832433191385398
1105	96-97	0.401947105195557
1105	98-99	0.39717885121734753
1105	100-101	0.44950171515723625
1106	1	0.139117146498986
1106	2	0.15112847292267162
1106	3	0.1963137080622559
1106	4	0.17538812307809337
1106	5	0.211885155177292
1106	6	0.2443265840950275
1106	7	0.24718275950236546
1106	8	0.23402373969393864
1106	9	0.3102939621709311
1106	10-11	0.289404506427978
1106	12-13	0.238559627322104
1106	14-15	0.2793148762458415
1106	16-17	0.315209655889106
1106	18-19	0.18736790671493964
1106	20-21	0.23114171429812558
1106	22-23	0.2577575502748388
1106	24-25	0.3357070910694233
1106	26-27	0.312767475362449
1106	28-29	0.2708808285857103
1106	30-31	0.30630499940553335
1106	32-33	0.27415887309349785
1106	34-35	0.3011112259331199
1106	36-37	0.3172352818186468
1106	38-39	0.2865784940194871
1106	40-41	0.19379111802427929
1106	42-43	0.1916934179557046
1106	44-45	0.23922432593174392
1106	46-47	0.2325023593910842
1106	48-49	0.23187669900686814
1106	50-51	0.16779817632942695
1106	52-53	0.2751892980135935
1106	54-55	0.2621181257084473
1106	56-57	0.14207935276883887
1106	58-59	0.22339532974850584
1106	60-61	0.11608661485816185
1106	62-63	0.2341815443175932
1106	64-65	0.3373844130399064
1106	66-67	0.3054226948836103
1106	68-69	0.2824699113518818
1106	70-71	0.23242789403337127
1106	72-73	0.12752764164515895
1106	74-75	0.18548074020168315
1106	76-77	0.31277805146993387
1106	78-79	0.3058373210813201
1106	80-81	0.3786990004458488
1106	82-83	0.35970423135980667
1106	84-85	0.3744034578193123
1106	86-87	0.2996888156342479
1106	88-89	0.3471530642060401
1106	90-91	0.32955189888480874
1106	92-93	0.4349559748985108
1106	94-95	0.4600899265303475
1106	96-97	0.5175020219662692
1106	98-99	0.5747777378738235
1106	100-101	0.5859841663388465
1107	1	0.18119931209855977
1107	2	0.3215744898217139
1107	3	0.35788360907720573
1107	4	0.32909842969653624
1107	5	0.2527384898575704
1107	6	0.2979195688658862
1107	7	0.37866556326748224
1107	8	0.3410451678316946
1107	9	0.4163606675112064
1107	10-11	0.374425473339592
1107	12-13	0.395855405929467
1107	14-15	0.5368810065840677
1107	16-17	0.4828957777272933
1107	18-19	0.45171483295396087
1107	20-21	0.4379055904956459
1107	22-23	0.43626784980141764
1107	24-25	0.43933431065643447
1107	26-27	0.4656598900292366
1107	28-29	0.4883245898644475
1107	30-31	0.5465433255117915
1107	32-33	0.5993303150323328
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2312	64-65	-0.2583135832346102
2312	66-67	-0.3237922097942416
2312	68-69	-0.18167935627533183
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2312	80-81	-0.07531876464783238
2312	82-83	-0.04005104555258043
2312	84-85	-0.017246011304571596
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2312	88-89	-0.06463150641553028
2312	90-91	-0.03053297156103696
2312	92-93	-0.005529144011347853
2312	94-95	0.04647535454974516
2312	96-97	0.05552221343597097
2312	98-99	0.10405133207094153
2312	100-101	0.181712150127737
2313	1	0.04647276470485551
2313	2	-0.08358722070727964
2313	3	-0.2439283833511965
2313	4	-0.12848181495551358
2313	5	-0.10906464869039212
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2313	24-25	-0.49643689286069304
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2313	28-29	-0.525093618598639
2313	30-31	-0.4314504564181476
2313	32-33	-0.6204240152275915
2313	34-35	-0.3325799093517432
2313	36-37	-0.27795062381360225
2313	38-39	-0.18707548113662398
2313	40-41	-0.4617976732354663
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2313	44-45	-0.27251797873539374
2313	46-47	-0.23865616649209898
2313	48-49	-0.6820534498124218
2313	50-51	-0.628629526882257
2313	52-53	-0.4660819792629596
2313	54-55	-0.4799970940511784
2313	56-57	-0.9113940252493222
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2313	60-61	-0.7297425848785792
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2313	64-65	-0.5111051860338023
2313	66-67	-0.4054564439308379
2313	68-69	-0.4221839005928274
2313	70-71	-0.5186002464029755
2313	72-73	-1.156113622993992
2313	74-75	-0.7366684467884284
2313	76-77	-1.0015707183213465
2313	78-79	-0.6538188356062662
2313	80-81	-0.556332987160971
2313	82-83	-0.4849512372185316
2313	84-85	-0.5182856477327249
2313	86-87	-0.489164871028656
2313	88-89	-0.4556761503762061
2313	90-91	-0.43450475548831946
2313	92-93	-0.31025303934608317
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2313	100-101	-0.6433223242990564
2314	1	0.0820588659216206
2314	2	-0.08828928695145777
2314	3	-0.1528576179244041
2314	4	-0.18129126077084123
2314	5	-0.09919996154692257
2314	6	-0.2294532839414387
2314	7	-0.20742291415447056
2314	8	-0.27854944098898216
2314	9	-0.33077628360302924
2314	10-11	-0.45420535389542493
2314	12-13	-0.5146988487529214
2314	14-15	-0.8086028956744968
2314	16-17	-0.5876324143748235
2314	18-19	-0.8186844373539444
2314	20-21	-0.46651858138203295
2314	22-23	-0.9127786026497873
2314	24-25	-0.5977856915091735
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2314	30-31	-0.4784676529553735
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2314	36-37	-0.9039472109981546
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2314	54-55	-0.40261195379998327
2314	56-57	-0.3746477165855211
2314	58-59	-0.5875817639601308
2314	60-61	-0.43500879525918634
2314	62-63	-0.8582932712620206
2314	64-65	-0.8382193286853123
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2314	78-79	-0.40686516860696287
2314	80-81	-0.4379997777898481
2314	82-83	-0.34897318983097136
2314	84-85	-0.4098157457136793
2314	86-87	-0.47870659434342855
2314	88-89	-0.42877601665470877
2314	90-91	-0.4238807888535163
2314	92-93	-0.3798206265219619
2314	94-95	-0.33240403640156657
2314	96-97	-0.2988892071060718
2314	98-99	-0.274733260484048
2314	100-101	-0.17513349695616398
2315	1	-0.1749778395337458
2315	2	-0.2951906852000583
2315	3	-0.3535999490334376
2315	4	-0.8672379600883033
2315	5	-0.780685367176261
2315	6	-0.6520700789365677
2315	7	-0.5195866793991684
2315	8	-0.4486885160836849
2315	9	-0.6906986956104575
2315	10-11	-0.8224525267086804
2315	12-13	-0.5435908768424298
2315	14-15	-0.5105728402554064
2315	16-17	-0.3582120559616371
2315	18-19	-0.33930024875876796
2315	20-21	-0.5620506795936535
2315	22-23	-0.7301256441906006
2315	24-25	-0.6031548682796739
2315	26-27	-0.42067504673415357
2315	28-29	-0.4236905512689688
2315	30-31	-0.3642701018919041
2315	32-33	-0.23683190715275515
2315	34-35	-0.3244877476522703
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2315	48-49	-1.0577790740040953
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2315	56-57	-0.7889705515113619
2315	58-59	-0.7425559478915105
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2315	68-69	-0.5405912210626269
2315	70-71	-0.5064622391207294
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2315	74-75	-0.4014352542204591
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2315	82-83	-0.4432363421743659
2315	84-85	-0.46593004932793036
2315	86-87	-0.4141879150230956
2315	88-89	-0.28218384255160345
2315	90-91	-0.30216012214702914
2315	92-93	-0.4168000162384864
2315	94-95	-0.3049856782094871
2315	96-97	-0.3247756932868846
2315	98-99	-0.5085571870172245
2315	100-101	-0.5274812310767096
2316	1	-0.27936645020859885
2316	2	-0.48933841856752736
2316	3	-0.5906494948028396
2316	4	-0.4193831372384622
2316	5	-0.4756493624660365
2316	6	-1.097352512305065
2316	7	-0.7263825414123701
2316	8	-0.5797264456401621
2316	9	-0.597980692449724
2316	10-11	-1.061452609210626
2316	12-13	-0.7728514192993714
2316	14-15	-0.998031367367588
2316	16-17	-0.8466915930198553
2316	18-19	-0.6913225681432991
2316	20-21	-0.7877285046098805
2316	22-23	-0.6324781578067658
2316	24-25	-0.6343692147248419
2316	26-27	-0.6226175868956645
2316	28-29	-0.6581131637815432
2316	30-31	-0.5935726114277458
2316	32-33	-0.7437852389554038
2316	34-35	-1.1598150170187012
2316	36-37	-0.7997877940547298
2316	38-39	-0.5900699751649157
2316	40-41	-0.6178813987847107
2316	42-43	-0.5860765372234695
2316	44-45	-0.5620143678683647
2316	46-47	-0.559116731187352
2316	48-49	-0.6118741016138998
2316	50-51	-0.6649330901900044
2316	52-53	-0.7791300205160425
2316	54-55	-0.7264822233857728
2316	56-57	-0.7119019721126492
2316	58-59	-0.6917671741820968
2316	60-61	-0.7244603089893218
2316	62-63	-0.6611379237090773
2316	64-65	-0.6948795225574074
2316	66-67	-0.5722828294109306
2316	68-69	-0.7119429086151143
2316	70-71	-0.6773947332915142
2316	72-73	-0.57406424965545
2316	74-75	-0.4960993930158679
2316	76-77	-0.628717368948557
2316	78-79	-0.6275277890822579
2316	80-81	-0.6601296667313363
2316	82-83	-0.5493163917292421
2316	84-85	-0.4367977354678878
2316	86-87	-0.40594240597048525
2316	88-89	-0.37911415885029953
2316	90-91	-0.42612207100090416
2316	92-93	-0.36801615892834505
2316	94-95	-0.5190562416827937
2316	96-97	-0.4832980761516623
2316	98-99	-0.39491160548823245
2316	100-101	-0.4303521045066532
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	20801.0
3	3221.0
4	2712.0
5	2853.0
6	3170.0
7	3758.0
8	4621.0
9	5396.0
10	6122.0
11	7309.0
12	8667.0
13	9917.0
14	11341.0
15	12551.0
16	13469.0
17	15944.0
18	15318.0
19	12092.0
20	12626.0
21	13268.0
22	14994.0
23	16900.0
24	20069.0
25	23433.0
26	27381.0
27	33044.0
28	40188.0
29	48385.0
30	60157.0
31	75478.0
32	97530.0
33	128724.0
34	182257.0
35	286569.0
36	503338.0
37	983538.0
38	1319937.0
39	384068.0
40	165.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.50312724232032	19.35192917234015	13.707276558959958	24.437667026379575
2	16.118557177510542	21.335690440314483	38.781860552050304	23.763891830124667
3	18.89210025146257	24.980739734317943	30.207373568700675	25.919786445518817
4	13.620235313261345	18.040969638894037	37.123966605947466	31.21482844189715
5	14.460971020253485	36.621168052610656	34.645692244732125	14.272168682403736
6	31.090285162765696	36.13842003393875	18.517290981966735	14.254003821328817
7	26.648133277721115	32.01743108611473	22.993791525583802	18.340644110580346
8	24.653156109402303	34.15102289324431	21.762622799714894	19.433198197638497
9	25.676392177451586	16.380976822287945	21.346912947977163	36.59571805228331
10-11	24.13852612219493	26.038696879264624	29.317844763199506	20.50493223534094
12-13	24.959875596274394	24.264284265133746	28.474861473659097	22.300978664932764
14-15	22.549450499787024	26.16453709677237	26.14451623982998	25.141496163610622
16-17	22.337351202801482	28.41849511874792	26.98061137771231	22.26354230073828
18-19	22.228360235571653	27.862062154786184	28.254248439938344	21.655329169703815
20-21	23.033161551260697	27.221822578678907	27.901817919288373	21.843197950772026
22-23	22.899859470003413	27.142523220099303	27.5018864053525	22.455730904544783
24-25	22.65002573154163	27.284052724583947	27.67156206045715	22.394359483417276
26-27	22.558480103582063	27.32401633979471	27.804626445552433	22.312877111070797
28-29	22.59245067143602	27.284086708738926	27.786861652277565	22.336600967547486
30-31	22.887948265168234	27.230073994201508	27.560346554062914	22.32163118656734
32-33	22.714741515663263	27.27292956294623	27.479522299760074	22.53280662163043
34-35	22.953140431389198	27.28325531802882	27.364358547900842	22.39924570268114
36-37	22.906213300359322	27.150144920331947	27.47064734847479	22.47299443083394
38-39	22.78906101033542	27.254959637008703	27.57259283759104	22.383386515064842
40-41	22.944889839889214	27.13194214517071	27.53101887356843	22.392149141371643
42-43	22.89390788398063	27.156905967885553	27.342980566483234	22.606205581650578
44-45	22.97875782602099	27.24349512579986	27.16978927968578	22.60795776849337
46-47	22.970347230671777	27.176943340212357	27.12782200543159	22.72488742368428
48-49	22.852603261397054	27.194112252141974	27.24142096061649	22.711863525844485
50-51	22.843263077979827	27.299763204511766	27.18219017750624	22.67478354000217
52-53	22.930503251537434	27.319691910742357	27.033435074132296	22.71636976358791
54-55	22.881901328767896	27.267616966610593	27.10567457960827	22.744807125013242
56-57	22.85681104486862	27.350248045006786	27.058940753546644	22.734000156577952
58-59	22.833727767004635	27.232663829666105	27.221944029540108	22.71166437378915
60-61	22.925231441658696	27.184388265105625	27.20864026267613	22.68174003055955
62-63	22.70196823595246	27.35144927618085	27.192382791541124	22.754199696325568
64-65	22.900941285785176	27.22845839370403	27.15784388250107	22.712756438009723
66-67	22.808030937617175	27.32174758086628	27.007737228635804	22.862484252880744
68-69	22.74743415757533	27.228320772443865	27.139531394541176	22.88471367543963
70-71	22.828157016384594	26.99030466423742	27.21160055898959	22.969937760388394
72-73	22.793789630564152	27.05457056173548	27.087692170569056	23.063947637131314
74-75	22.71787355957036	27.196264827844193	27.07806675158706	23.007794860998384
76-77	22.689679053128494	27.16616966795892	27.096282931695487	23.047868347217097
78-79	22.661211303474573	27.162462112238266	27.086678115608553	23.089648468678604
80-81	22.67528648919304	27.193214256622777	27.07665617376543	23.05484308041875
82-83	22.718374226498835	27.182522040030836	27.12668544984553	22.972418283624798
84-85	22.72957736359095	27.10860101571197	27.065174437245094	23.09664718345199
86-87	22.685405501165754	27.100537751764676	27.130907462316927	23.083149284752643
88-89	22.648354194463714	27.045995479721274	27.145214773112258	23.16043555270275
90-91	22.709150203482718	26.97866069485772	27.175839299832365	23.1363498018272
92-93	22.675977115811204	26.999643394988187	27.20238622537437	23.12199326382624
94-95	22.568962340573183	26.949279274363754	27.266780151823394	23.214978233239673
96-97	22.598409650975746	26.941012151593004	27.29557762252738	23.16500057490388
98-99	22.52825842313983	26.922533442988588	27.205640084303973	23.343568049567608
100-101	22.552126521611683	26.849545965832007	27.278041488771926	23.32028602378439
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7476.0
1	4258.5
2	982.0
3	880.5
4	728.0
5	695.5
6	945.0
7	1485.5
8	2093.5
9	2161.0
10	1646.0
11	1202.0
12	1149.0
13	1226.0
14	1254.0
15	1334.0
16	1447.0
17	1555.0
18	1694.5
19	1898.5
20	2122.5
21	2362.5
22	2724.5
23	3236.0
24	3936.0
25	5035.0
26	7008.5
27	10009.0
28	14689.0
29	22383.5
30	33599.0
31	48834.5
32	67184.5
33	85343.5
34	102227.0
35	116843.5
36	130505.5
37	141152.5
38	145480.5
39	148255.5
40	152392.0
41	153840.5
42	153335.0
43	156998.0
44	162551.5
45	167896.5
46	171748.0
47	173754.5
48	174700.0
49	173559.5
50	169993.5
51	164967.5
52	159844.0
53	154775.5
54	147564.5
55	136649.0
56	123439.0
57	110807.0
58	100728.0
59	88555.5
60	73090.5
61	59163.0
62	46674.5
63	34536.0
64	24780.5
65	17793.0
66	12993.0
67	9795.5
68	7578.0
69	5760.5
70	4225.5
71	3062.5
72	2157.0
73	1553.5
74	1174.5
75	894.5
76	657.5
77	461.0
78	348.0
79	264.0
80	201.0
81	150.0
82	113.5
83	99.5
84	73.0
85	51.5
86	45.0
87	38.5
88	31.5
89	27.0
90	27.5
91	18.5
92	8.0
93	11.5
94	13.5
95	8.0
96	9.0
97	9.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1463675196798419
2	0.15070032322263097
3	0.14438165138939696
4	0.1482856879149308
5	0.13648331159785446
6	0.11513522747557099
7	0.1224242667689088
8	0.11048649034112028
9	0.10773335475663974
10-11	0.09616792863330964
12-13	0.11457106034760368
14-15	0.13373017601337392
16-17	0.14376106754863288
18-19	0.1409402319087963
20-21	0.131778157750607
22-23	0.12559488602808513
24-25	0.1091550559191174
26-27	0.11423256007082327
28-29	0.11903926400110487
30-31	0.1086698721890655
32-33	0.10668400389862052
34-35	0.12343976759924997
36-37	0.1264524200625955
38-39	0.11235952520597177
40-41	0.1149659773371808
42-43	0.10968537301940667
44-45	0.12661038685842632
46-47	0.12491788547452437
48-49	0.13814196295407838
50-51	0.1252676690938641
52-53	0.10982077313011884
54-55	0.09992528170557201
56-57	0.12187138298350082
58-59	0.11108450749676563
60-61	0.10993360655571228
62-63	0.10967408967684732
64-65	0.1325792750723206
66-67	0.12415061818048881
68-69	0.12793053793786985
70-71	0.11532704429907989
72-73	0.11729034590440618
74-75	0.09974474822462245
76-77	0.12588825293462813
78-79	0.1257979861941534
80-81	0.12683605370961326
82-83	0.13308702548749118
84-85	0.12298843389687611
86-87	0.13199254125923457
88-89	0.11862178032640905
90-91	0.12700530384800343
92-93	0.14097408193647432
94-95	0.11692927894250708
96-97	0.10118901607221881
98-99	0.10630037025160274
100-101	0.1499330559285954
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4431311.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.132358536832534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.55872740493086	23.829525814115232
2	18.871643563018186	15.902137055527799
3	9.229187440505486	11.665423027410267
4	5.038728799582063	8.49174113415421
5	2.9285638087069974	6.169365019321753
6	1.919533299496777	4.852467911867241
7	1.3144339949325485	3.8766143043249475
8	0.9264434210788658	3.122659710478759
9	0.6318329047457291	2.3958549430303875
>10	2.506120398107057	16.54160137455199
>50	0.05734810170881004	1.6231089321451158
>100	0.017061125901566634	1.1239861617399463
>500	1.0735353207791184E-4	0.03496323201698083
>1k	2.683837529316015E-4	0.37055137931525783
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4970	0.11215642503990356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	9.026674047477146E-5
9	0.0	0.0	0.0	0.0	9.026674047477146E-5
10-11	0.0	0.0	0.0	1.1283342559346432E-5	9.026674047477146E-5
12-13	0.0	0.0	0.0	2.2566685118692864E-5	9.026674047477146E-5
14-15	0.0	0.0	0.0	2.2566685118692864E-5	9.026674047477146E-5
16-17	0.0	0.0	0.0	2.2566685118692864E-5	9.026674047477146E-5
18-19	0.0	0.0	0.0	2.2566685118692864E-5	9.026674047477146E-5
20-21	0.0	0.0	0.0	4.513337023738573E-5	9.026674047477146E-5
22-23	0.0	0.0	0.0	1.2411676815281074E-4	9.026674047477146E-5
24-25	0.0	0.0	0.0	3.4978361933973943E-4	9.026674047477146E-5
26-27	0.0	0.0	0.0	8.34967349391636E-4	9.026674047477146E-5
28-29	0.0	0.0	0.0	0.002550035418412294	9.026674047477146E-5
30-31	0.0	0.0	0.0	0.006149421694843806	9.026674047477146E-5
32-33	0.0	0.0	0.0	0.015503312676541998	9.026674047477146E-5
34-35	2.2566685118692864E-5	0.0	0.0	0.029934707809946087	9.026674047477146E-5
36-37	2.2566685118692864E-5	0.0	0.0	0.049240506928987834	9.026674047477146E-5
38-39	2.2566685118692864E-5	0.0	0.0	0.07459417765983926	9.026674047477146E-5
40-41	2.2566685118692864E-5	0.0	0.0	0.10011709852908089	9.026674047477146E-5
42-43	2.2566685118692864E-5	0.0	0.0	0.12938608912802554	1.354001107121572E-4
44-45	2.2566685118692864E-5	0.0	0.0	0.16802025405122772	1.8053348094954291E-4
46-47	2.2566685118692864E-5	0.0	0.0	0.21305207420557934	1.8053348094954291E-4
48-49	2.2566685118692864E-5	0.0	0.0	0.256978126789115	1.8053348094954291E-4
50-51	2.2566685118692864E-5	0.0	0.0	0.29728222641110047	1.8053348094954291E-4
52-53	2.2566685118692864E-5	0.0	0.0	0.33833102664200276	1.8053348094954291E-4
54-55	2.2566685118692864E-5	0.0	0.0	0.38783105044985555	1.8053348094954291E-4
56-57	2.2566685118692864E-5	0.0	0.0	0.4493591174259717	1.8053348094954291E-4
58-59	2.2566685118692864E-5	0.0	0.0	0.5138998368654333	1.8053348094954291E-4
60-61	2.2566685118692864E-5	0.0	0.0	0.577628155640622	1.8053348094954291E-4
62-63	2.2566685118692864E-5	0.0	0.0	0.6385582054610927	1.8053348094954291E-4
64-65	2.2566685118692864E-5	0.0	0.0	0.7087857295504649	1.8053348094954291E-4
66-67	2.2566685118692864E-5	0.0	0.0	0.7965814180047395	1.8053348094954291E-4
68-69	2.2566685118692864E-5	0.0	0.0	0.906943791577707	1.8053348094954291E-4
70-71	2.2566685118692864E-5	0.0	0.0	1.0164937644864014	1.8053348094954291E-4
72-73	2.2566685118692864E-5	0.0	0.0	1.134382127546453	1.8053348094954291E-4
74-75	2.2566685118692864E-5	0.0	0.0	1.2633055996295453	1.8053348094954291E-4
76-77	2.2566685118692864E-5	0.0	0.0	1.4054418658496322	1.8053348094954291E-4
78-79	2.2566685118692864E-5	0.0	0.0	1.563498928420957	1.8053348094954291E-4
80-81	2.2566685118692864E-5	0.0	0.0	1.7326813667557976	1.8053348094954291E-4
82-83	2.2566685118692864E-5	2.2566685118692864E-5	0.0	1.9182585018293683	1.8053348094954291E-4
84-85	2.2566685118692864E-5	2.2566685118692864E-5	0.0	2.1319650099033898	1.8053348094954291E-4
86-87	2.2566685118692864E-5	2.2566685118692864E-5	0.0	2.3892365035990477	1.8053348094954291E-4
88-89	2.2566685118692864E-5	2.2566685118692864E-5	0.0	2.6721550349321	1.8053348094954291E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4725	0.0	51.986084	1
GTATCAA	8510	0.0	38.299484	1
TCAACGC	9745	0.0	33.395462	4
ATCAACG	9830	0.0	32.9617	3
CAACGCA	9990	0.0	32.574986	5
TATCAAC	10090	0.0	32.255054	2
AACGCAG	10400	0.0	31.373981	6
ACGCAGA	11925	0.0	26.963821	7
CGCAGAG	12285	0.0	25.634731	8
GTGGTAT	2665	0.0	21.03697	1
GTACATG	9435	0.0	20.49514	1
GCAGAGT	14640	0.0	20.472868	9
CAGAGTA	13425	0.0	18.612347	10-11
TACATGG	9860	0.0	18.599821	2
GAGTACT	7450	0.0	18.397797	12-13
AGAGTAC	11810	0.0	17.939678	10-11
TGGTATC	2915	0.0	17.928854	2
ACATGGG	10185	0.0	16.979273	3
ACTATCG	170	0.0017863063	16.767992	3
GTACTTT	8160	0.0	16.390104	14-15
>>END_MODULE