FastQCFastQC Report
Fri 10 Feb 2017
SRR2031714_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031714_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4431311
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74100.16721913672951413No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT60120.13567091093358152No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59550.13438460988181603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50450.052.9344671
GTATCAA91250.040.3061681
TCAACGC103050.035.0826384
ATCAACG104800.034.451873
CAACGCA104650.034.448085
TATCAAC108500.033.6272962
AACGCAG109850.032.7741626
ACGCAGA125650.028.3127337
CGCAGAG128850.027.4252748
GCAGAGT151800.022.6531839
GTGGTAT27150.022.2278421
GTACATG86350.021.4617771
CAGAGTA141300.020.20224610-11
GAGTACT92950.019.87787612-13
TACATGG90750.019.6834722
TGGTATC28650.019.2350732
ACATGGG90100.019.087293
AGAGTAC131150.018.70550210-11
GTACTTT102650.017.69883714-15
CATGGGG62700.015.7598244
Produced by FastQC (version 0.11.5)