##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031714_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4431311 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.72991175749118 34.0 31.0 34.0 31.0 34.0 2 32.89460274848685 34.0 31.0 34.0 31.0 34.0 3 32.959681232032686 34.0 31.0 34.0 31.0 34.0 4 36.35737550354737 37.0 37.0 37.0 35.0 37.0 5 36.318933381114526 37.0 37.0 37.0 35.0 37.0 6 36.342571532442655 37.0 37.0 37.0 35.0 37.0 7 36.28704868604348 37.0 37.0 37.0 35.0 37.0 8 36.27600500168009 37.0 37.0 37.0 35.0 37.0 9 38.12894581310136 39.0 39.0 39.0 37.0 39.0 10-11 38.0891078283605 39.0 39.0 39.0 36.0 39.0 12-13 38.03922586340701 39.0 39.0 39.0 35.0 39.0 14-15 39.537450542288724 41.0 39.0 41.0 37.0 41.0 16-17 39.511760063782475 41.0 39.0 41.0 37.0 41.0 18-19 39.50522396193813 41.0 39.5 41.0 36.5 41.0 20-21 39.49079572614063 41.0 39.0 41.0 36.5 41.0 22-23 39.40163937940713 41.0 39.0 41.0 36.0 41.0 24-25 39.3372372194143 41.0 39.0 41.0 36.0 41.0 26-27 39.204597239959014 41.0 39.0 41.0 36.0 41.0 28-29 39.10734295110409 40.5 39.0 41.0 36.0 41.0 30-31 38.96022238114183 40.0 38.5 41.0 35.0 41.0 32-33 38.7827992664022 40.0 38.0 41.0 35.0 41.0 34-35 38.65387726115364 40.0 38.0 41.0 35.0 41.0 36-37 38.56266305840416 40.0 38.0 41.0 34.5 41.0 38-39 38.649470326050235 40.0 38.0 41.0 35.0 41.0 40-41 38.58737041927321 40.0 38.0 41.0 35.0 41.0 42-43 38.507324807489255 40.0 38.0 41.0 35.0 41.0 44-45 38.352880445538574 40.0 38.0 41.0 34.0 41.0 46-47 38.1869664530429 40.0 38.0 41.0 34.0 41.0 48-49 37.9932119411163 40.0 38.0 41.0 33.0 41.0 50-51 37.87428494637366 40.0 38.0 41.0 33.0 41.0 52-53 37.951158584897335 40.0 38.0 41.0 33.5 41.0 54-55 37.87868341897014 40.0 37.5 41.0 34.0 41.0 56-57 37.698606800560825 40.0 37.0 41.0 33.0 41.0 58-59 37.514453284817975 40.0 37.0 41.0 33.0 41.0 60-61 37.2506921766493 39.5 36.0 41.0 33.0 41.0 62-63 36.973905013663 39.0 36.0 41.0 32.0 41.0 64-65 36.65006044486609 39.0 35.0 41.0 32.0 41.0 66-67 36.29982233248806 38.5 35.0 40.5 31.5 41.0 68-69 35.914156555475344 37.0 35.0 40.0 31.0 41.0 70-71 35.556405655121026 37.0 35.0 39.5 31.0 41.0 72-73 35.16340457711048 36.5 35.0 39.0 30.5 41.0 74-75 34.77370805163528 36.0 35.0 39.0 30.0 40.5 76-77 33.82945509805112 35.0 33.5 37.0 29.0 39.0 78-79 34.02883051990709 35.0 34.0 37.0 30.0 39.0 80-81 33.80415423336345 35.0 34.0 37.0 30.0 39.0 82-83 33.535337849227915 35.0 34.0 36.0 30.0 38.0 84-85 33.24621618748944 35.0 34.0 36.0 29.0 37.0 86-87 32.998457228571866 35.0 34.0 35.5 29.0 37.0 88-89 32.77497291433619 35.0 34.0 35.0 29.0 36.0 90-91 32.57833979605584 35.0 34.0 35.0 29.0 36.0 92-93 32.40932751052679 35.0 34.0 35.0 29.0 36.0 94-95 32.234358635627245 35.0 34.0 35.0 29.0 36.0 96-97 32.0701998799001 35.0 34.0 35.0 27.5 35.0 98-99 31.860078089757188 35.0 33.0 35.0 27.0 35.0 100-101 31.144341482689885 34.5 32.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13472308106502595 1101 2 0.15754147931102835 1101 3 0.17157353292056143 1101 4 0.1150569484167221 1101 5 0.07027965732447683 1101 6 0.10447903829235372 1101 7 0.16241558665019795 1101 8 0.09434123296819052 1101 9 0.10220826039049058 1101 10-11 0.1258228872096936 1101 12-13 0.12052424622137181 1101 14-15 0.10732845340587005 1101 16-17 0.07512230316000768 1101 18-19 0.09900932656933747 1101 20-21 0.09498973313603898 1101 22-23 0.012182297649687257 1101 24-25 -0.010017064421894872 1101 26-27 0.09789801328230396 1101 28-29 0.054414192216455604 1101 30-31 0.17342073585622586 1101 32-33 0.1731899740977454 1101 34-35 -0.19920931451370194 1101 36-37 0.02867810854623798 1101 38-39 0.06575989762111334 1101 40-41 -0.19467481844064594 1101 42-43 -0.16762672637816678 1101 44-45 -0.08007946115587572 1101 46-47 -0.04952095824375391 1101 48-49 -0.23140846235677515 1101 50-51 -0.1678434111834406 1101 52-53 -0.17109813984162514 1101 54-55 -0.04643590330870495 1101 56-57 -0.29227312667927663 1101 58-59 -0.15739239281678152 1101 60-61 -0.18032698991118679 1101 62-63 -0.19603034736940117 1101 64-65 -0.1138540333237188 1101 66-67 -0.35581700401058924 1101 68-69 -0.3892017578314153 1101 70-71 -0.3445879669132097 1101 72-73 -0.4259703028589996 1101 74-75 -0.411522848251785 1101 76-77 -0.43200331734011144 1101 78-79 -0.49081853632633 1101 80-81 -0.4359678717307318 1101 82-83 -0.38145314957143484 1101 84-85 -0.32959507111257835 1101 86-87 -0.2655626852799031 1101 88-89 -0.31458813752802683 1101 90-91 -0.3796706581163889 1101 92-93 -0.5169150297961167 1101 94-95 -0.4805035261843855 1101 96-97 -0.33195741676692947 1101 98-99 -0.6246708424593983 1101 100-101 -0.8961607993787766 1102 1 0.15623771545805454 1102 2 0.09279636802249769 1102 3 0.14946488628001475 1102 4 0.057042517383763425 1102 5 0.11555800955559192 1102 6 0.10851847938571524 1102 7 0.133091710456668 1102 8 0.088555118981823 1102 9 0.10122381115913726 1102 10-11 0.1830673833967822 1102 12-13 0.21028501300718005 1102 14-15 0.23878814830121087 1102 16-17 0.20901618578789538 1102 18-19 0.23199085057191127 1102 20-21 0.2041129607293115 1102 22-23 0.1711535969806448 1102 24-25 0.10857205427223704 1102 26-27 0.17477314015818024 1102 28-29 0.20724125581902086 1102 30-31 0.011495012852002162 1102 32-33 0.11128562721352608 1102 34-35 0.16095940619725013 1102 36-37 0.05455010001875138 1102 38-39 -0.004495482454196065 1102 40-41 0.16371150686804015 1102 42-43 0.18100114908656195 1102 44-45 0.13736553299438015 1102 46-47 0.17293817068652828 1102 48-49 0.21045885649675 1102 50-51 0.20030595481375002 1102 52-53 0.17895903051959294 1102 54-55 0.34046132417984154 1102 56-57 0.4013904677081257 1102 58-59 0.3586770695307564 1102 60-61 0.317651747305284 1102 62-63 0.3077042688180569 1102 64-65 0.2614249860144966 1102 66-67 0.2506741991421748 1102 68-69 0.2838466849038923 1102 70-71 0.1759615141420241 1102 72-73 0.216368581809661 1102 74-75 0.20189235275189077 1102 76-77 0.1902666903136918 1102 78-79 0.23222951188326846 1102 80-81 0.09004700977884994 1102 82-83 0.14878376670088045 1102 84-85 0.17618785080217236 1102 86-87 0.2013764551848709 1102 88-89 0.1400137537564703 1102 90-91 0.02494995520610388 1102 92-93 0.1395994145072521 1102 94-95 0.12166955726353734 1102 96-97 -0.061600787049385985 1102 98-99 -0.25229696694731985 1102 100-101 -0.20580164469672013 1103 1 0.1870978861801973 1103 2 0.1392642311929464 1103 3 0.1709176019049039 1103 4 0.11843973375570727 1103 5 0.0584733924889278 1103 6 0.0946931537727238 1103 7 0.13622303815342462 1103 8 0.15942051603030194 1103 9 0.12363080522941772 1103 10-11 0.12219926486749699 1103 12-13 0.13461139573268355 1103 14-15 0.17878619858361589 1103 16-17 0.17713089011118655 1103 18-19 0.17417376928562334 1103 20-21 0.17086438595256936 1103 22-23 0.14511156431881034 1103 24-25 0.030824492603954923 1103 26-27 0.0896432571480048 1103 28-29 0.14645914826907358 1103 30-31 0.19755138406198824 1103 32-33 0.054079457440515455 1103 34-35 0.07176629140830215 1103 36-37 0.10954174940536632 1103 38-39 0.06766583365934054 1103 40-41 0.06107271980901885 1103 42-43 0.05917839330967922 1103 44-45 0.10744684569591811 1103 46-47 0.06106175028680383 1103 48-49 0.12767415203128252 1103 50-51 0.02113916121234638 1103 52-53 0.09964780210950863 1103 54-55 0.21313975549664121 1103 56-57 0.12311360510161506 1103 58-59 0.11397444546812352 1103 60-61 -0.0813536259268588 1103 62-63 -0.15794891704079816 1103 64-65 0.023096855313013975 1103 66-67 0.043717803086828155 1103 68-69 0.07516455183160531 1103 70-71 -0.014850035497580905 1103 72-73 0.021062086880618835 1103 74-75 0.10778852216609636 1103 76-77 0.06319509844374238 1103 78-79 0.058693611015577574 1103 80-81 0.005392776623772022 1103 82-83 0.12163635247998883 1103 84-85 -0.023233766827061686 1103 86-87 -0.03317365763471969 1103 88-89 0.11148332631473323 1103 90-91 -0.016701767010587787 1103 92-93 -0.04833779104646396 1103 94-95 -0.010899425843653887 1103 96-97 -0.011090238603308933 1103 98-99 0.12855849872715552 1103 100-101 0.0654578254810616 1104 1 0.1482433613491736 1104 2 0.11435401363093689 1104 3 0.12565948499182866 1104 4 0.10294404409083313 1104 5 0.13451807410249472 1104 6 0.11180884034190797 1104 7 0.16834986863192114 1104 8 0.15867115814184984 1104 9 0.0859198341039118 1104 10-11 0.14730146801054644 1104 12-13 0.2025164160189803 1104 14-15 0.21924621931457722 1104 16-17 0.14659836628758427 1104 18-19 0.16792441515906376 1104 20-21 0.22001568493067225 1104 22-23 0.1938674135127414 1104 24-25 0.2258041934485604 1104 26-27 0.18844911390753794 1104 28-29 0.21709997112358792 1104 30-31 0.18926156821059692 1104 32-33 0.16529585891886 1104 34-35 0.09665995017375195 1104 36-37 0.13784799929966596 1104 38-39 0.15514257980373003 1104 40-41 0.10155949183850055 1104 42-43 0.19448395326484302 1104 44-45 0.12714756940214045 1104 46-47 0.003969174010073573 1104 48-49 0.030486555913903146 1104 50-51 0.07866821450510741 1104 52-53 0.09021086356926133 1104 54-55 0.1456527564026473 1104 56-57 0.02701046547018393 1104 58-59 0.04014398040774125 1104 60-61 0.16698321176227893 1104 62-63 0.21370970269379796 1104 64-65 0.14163079798434097 1104 66-67 0.051444619855296025 1104 68-69 0.04981978643373708 1104 70-71 0.06780167024816564 1104 72-73 0.0723621388977449 1104 74-75 0.056910639078658676 1104 76-77 0.14302917222858014 1104 78-79 0.2298869382128288 1104 80-81 0.2070718450252329 1104 82-83 0.19671406468064845 1104 84-85 0.2216944988586036 1104 86-87 0.2558385937526282 1104 88-89 0.24077730360069438 1104 90-91 0.22354103446256346 1104 92-93 0.22725894135958669 1104 94-95 0.2741477815169233 1104 96-97 0.2855665500177693 1104 98-99 0.3928613280563802 1104 100-101 0.41200411387664815 1105 1 0.14276016767373534 1105 2 0.10438353018991364 1105 3 0.06064811325325081 1105 4 0.07021808539334273 1105 5 0.08014255741504428 1105 6 0.0815835935317395 1105 7 0.07760010914770277 1105 8 0.16140038770625864 1105 9 0.06917405380526276 1105 10-11 0.14341112991514393 1105 12-13 0.17136168555538944 1105 14-15 0.14451235565288556 1105 16-17 0.18265047545529 1105 18-19 0.18100828274629777 1105 20-21 0.12806633069922668 1105 22-23 0.13463755746287376 1105 24-25 0.20910871288092636 1105 26-27 0.204311710916798 1105 28-29 0.23280136287868913 1105 30-31 0.26312830757959915 1105 32-33 0.16600239324463217 1105 34-35 0.1499844947442952 1105 36-37 0.2144712562217066 1105 38-39 0.18956273188796757 1105 40-41 0.17188620689627498 1105 42-43 0.09981127622639718 1105 44-45 0.2221634228928906 1105 46-47 0.17886389221221322 1105 48-49 0.20403702534114387 1105 50-51 0.25646441193158864 1105 52-53 0.2954723754087425 1105 54-55 0.23637811974821687 1105 56-57 0.16428141556965414 1105 58-59 0.2273882208885638 1105 60-61 0.13824188392813852 1105 62-63 0.11472364682522596 1105 64-65 0.15401145379684777 1105 66-67 0.24011546530670813 1105 68-69 0.1978170388070879 1105 70-71 0.2382529071354753 1105 72-73 0.14641531029732846 1105 74-75 0.10721858418421704 1105 76-77 0.055912960714486815 1105 78-79 0.15932730617428348 1105 80-81 0.06715439072937812 1105 82-83 0.07317663318980294 1105 84-85 0.14481531321340668 1105 86-87 0.029204649291415308 1105 88-89 0.01917157867296737 1105 90-91 0.04436339414815649 1105 92-93 0.038432951233225765 1105 94-95 -0.037852651399170156 1105 96-97 0.03167805778405608 1105 98-99 0.12111747729847977 1105 100-101 0.06422663594884881 1106 1 0.1258632541737228 1106 2 0.0553743847823327 1106 3 0.025007402579618088 1106 4 0.05089679922715362 1106 5 0.0636486549540507 1106 6 0.08003845761671613 1106 7 0.06683346803509949 1106 8 0.021101433997387176 1106 9 0.05560753704348542 1106 10-11 0.07983304535513724 1106 12-13 0.07648480873118757 1106 14-15 0.11976786825529473 1106 16-17 0.11388402918139917 1106 18-19 0.1818501313645413 1106 20-21 0.14321092697586835 1106 22-23 0.09198256278070005 1106 24-25 0.12275729133907021 1106 26-27 0.12897807663652117 1106 28-29 0.16013673076710688 1106 30-31 0.12357996943855909 1106 32-33 0.19398102921503124 1106 34-35 0.18560784771445782 1106 36-37 0.19955246024331075 1106 38-39 0.1352997880262663 1106 40-41 0.22796528306764685 1106 42-43 0.10298260072367782 1106 44-45 0.18441576329992415 1106 46-47 0.11066235209741393 1106 48-49 0.14043080208191583 1106 50-51 0.08249478683155331 1106 52-53 0.20115130546307824 1106 54-55 0.1334854074130476 1106 56-57 0.29968819965688454 1106 58-59 0.24850242568832215 1106 60-61 0.21105027265996767 1106 62-63 0.2791670459368447 1106 64-65 0.3882418000115564 1106 66-67 0.26576261334593454 1106 68-69 0.36379812623778207 1106 70-71 0.2988946568688604 1106 72-73 0.3101460525559787 1106 74-75 0.33658276730185577 1106 76-77 0.3130248442667636 1106 78-79 0.24515415269158325 1106 80-81 0.2600628319067013 1106 82-83 0.18842043264574926 1106 84-85 0.29900298230085554 1106 86-87 0.2466978449515267 1106 88-89 0.29299019175282126 1106 90-91 0.347016426028425 1106 92-93 0.30157501508698914 1106 94-95 0.41171538202947744 1106 96-97 0.4199140874754548 1106 98-99 0.4113460572317287 1106 100-101 0.432657495951581 1107 1 0.27743936949658377 1107 2 0.22825873947135022 1107 3 0.18935205840237046 1107 4 0.12528316183151134 1107 5 0.12061174104670158 1107 6 0.11092153985221387 1107 7 0.17484380276588496 1107 8 0.14166015538057763 1107 9 0.1734109552189551 1107 10-11 0.19150822098076503 1107 12-13 0.18678099346245602 1107 14-15 0.23620763360139563 1107 16-17 0.27452114625274504 1107 18-19 0.2588184228329311 1107 20-21 0.25537618725995515 1107 22-23 0.2683316619866716 1107 24-25 0.3236129512827475 1107 26-27 0.32152056913522387 1107 28-29 0.2960467824866839 1107 30-31 0.3125801661229346 1107 32-33 0.3018980919290257 1107 34-35 0.2564438149857793 1107 36-37 0.31189629259373675 1107 38-39 0.2905529254099193 1107 40-41 0.38483433821222945 1107 42-43 0.42528155153625846 1107 44-45 0.39859879864749814 1107 46-47 0.3597029736710695 1107 48-49 0.30916447230421085 1107 50-51 0.36550676697638806 1107 52-53 0.41351370293082823 1107 54-55 0.3712673790833563 1107 56-57 0.4859684567802489 1107 58-59 0.40770953127492504 1107 60-61 0.41106571914370704 1107 62-63 0.41886221959308045 1107 64-65 0.4655400671482255 1107 66-67 0.49886829280499256 1107 68-69 0.4003812926062267 1107 70-71 0.547292132012906 1107 72-73 0.45801131278903995 1107 74-75 0.5702254384746652 1107 76-77 0.5954599090858679 1107 78-79 0.6143879331183513 1107 80-81 0.5456454844978182 1107 82-83 0.6341908384584158 1107 84-85 0.6034268692739246 1107 86-87 0.5879514234081853 1107 88-89 0.6530884165934197 1107 90-91 0.57507706195927 1107 92-93 0.6396334609589971 1107 94-95 0.6567347201087514 1107 96-97 0.6726367375053286 1107 98-99 0.725754833181437 1107 100-101 0.7560011252400543 1108 1 0.0292318660328732 1108 2 0.014643159411242834 1108 3 -0.020514630700098735 1108 4 0.030828082235409227 1108 5 0.05384520593540287 1108 6 0.04177575377610765 1108 7 0.001992722569553962 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2316 94-95 -0.15916748628691835 2316 96-97 -0.16266789721796826 2316 98-99 -0.25489259373197015 2316 100-101 0.02796232581252056 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 8.0 4 16.0 5 42.0 6 81.0 7 203.0 8 568.0 9 1219.0 10 2160.0 11 3325.0 12 4771.0 13 6697.0 14 8299.0 15 9791.0 16 11697.0 17 15680.0 18 15139.0 19 10286.0 20 9095.0 21 9763.0 22 11027.0 23 12168.0 24 14446.0 25 17052.0 26 19820.0 27 23845.0 28 30704.0 29 39716.0 30 51402.0 31 66047.0 32 86335.0 33 115763.0 34 163965.0 35 256873.0 36 461436.0 37 992986.0 38 1508727.0 39 449915.0 40 240.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.71316826366727 19.958862365881178 14.167836401050629 24.160132969400923 2 15.960716817212784 21.543985515798823 39.12169558850643 23.373602078481966 3 18.542574241338286 25.30580715396017 30.802708235918562 25.348910368782985 4 13.444796623057762 18.36081432482936 37.324447688610604 30.869941363502274 5 13.967446654048882 36.78951894823 35.1438208692642 14.099213528456927 6 30.615499566606815 36.63141675228843 18.554870105032123 14.198213576072636 7 26.132808101259425 32.263837947731496 23.243663105568533 18.35969084544055 8 24.27261368024045 34.466278715260565 21.971285698521275 19.28982190597771 9 25.21547235118456 16.319211177008338 21.65059053629953 36.814725935507575 10-11 23.723122927883956 26.161882847496887 29.684938504069734 20.430055720549422 12-13 24.56849380527359 24.3064042355877 28.96605393958603 22.159048019552685 14-15 22.12426266110653 26.170630922208733 26.623025667145157 25.082080749539582 16-17 21.85915869921334 28.613393445697238 27.460571571452526 22.066876283636898 18-19 21.72070323761321 27.839826850084435 28.937483307680615 21.50198660462173 20-21 22.4694227058313 27.123203494406056 28.738854032136313 21.66851976762633 22-23 22.299800217136646 27.157414589046 28.303599092909526 22.239186100907833 24-25 22.16611517449351 27.269006846957932 28.5078275932337 22.057050385314867 26-27 22.061477944434547 27.307414432773797 28.644054849410328 21.987052773381336 28-29 21.948904649070535 27.15442110633135 28.710930557719884 22.185743686878233 30-31 22.403602097123702 27.055344274799253 28.51564690556907 22.025406722507974 32-33 22.179103443583536 27.209952746367065 28.320134985234013 22.290808824815382 34-35 22.348305704541254 27.261126644442662 28.349735019306355 22.04083263170973 36-37 22.40238161705416 27.08801660623406 28.283993142120018 22.22560863459176 38-39 22.227079074341656 27.08073299301268 28.46875337795068 22.223434554694986 40-41 22.425643336701036 26.99337735491822 28.38771641168945 22.193262896691294 42-43 22.51199475730771 26.956255157897967 28.15173658540328 22.380013499391037 44-45 22.457879846393087 27.116693005749315 28.06146984492851 22.363957302929087 46-47 22.537359711381125 26.980198410808903 27.888812137085388 22.593629740724587 48-49 22.43949928136391 27.02748689947512 27.95120902143858 22.58180479772239 50-51 22.442342683688867 27.201069841408103 27.851814056833295 22.504773418069732 52-53 22.4804690981969 27.238383855251865 27.826945569832496 22.45420147671874 54-55 22.510076589072625 27.17274865158415 27.713988027470876 22.603186731872352 56-57 22.49015020611282 27.390505879636972 27.60139155207116 22.51795236217905 58-59 22.38000892966249 27.313946465127547 27.863074446463038 22.442970158746924 60-61 22.584507770915756 27.318685120365444 27.698184332153943 22.39862277656486 62-63 22.34983358915038 27.49535591068273 27.594805210046445 22.56000529012045 64-65 22.460600292989323 27.361632616032676 27.65033418001523 22.527432910962773 66-67 22.415785285596353 27.419278763760087 27.498141550860574 22.66679439978299 68-69 22.331227536319044 27.33502957056252 27.59888702344669 22.73485586967175 70-71 22.44773266565944 27.07512798148627 27.656548127470348 22.820591225383943 72-73 22.473736865611784 27.10015366661544 27.478903846314427 22.947205621458348 74-75 22.451192862750993 27.143836591806526 27.526487436536108 22.878483108906373 76-77 22.388637686627835 27.224388718702226 27.422346606614322 22.964626988055613 78-79 22.414927473192076 27.15661401272879 27.416447909949525 23.012010604129607 80-81 22.413409830945152 27.184129137790485 27.413378235282966 22.989082795981393 82-83 22.489425027294345 27.106968451401958 27.44801943188262 22.95558708942108 84-85 22.534065110736996 27.022084211571528 27.35848141143974 23.085369266251732 86-87 22.38418726145913 27.104155441905338 27.399298728628292 23.112358568007245 88-89 22.42284218946102 26.986917922235538 27.39284924045281 23.197390647850632 90-91 22.49093383220763 26.93677490403515 27.384577021242023 23.18771424251519 92-93 22.427347121427495 26.907623951467187 27.445207524364683 23.219821402740635 94-95 22.367782356056708 26.841526130754534 27.484834623433112 23.30585688975565 96-97 22.40191952266937 26.828545057782012 27.421473559737414 23.348061859811207 98-99 22.355939891363956 26.76288727261239 27.395826516312333 23.48534631971133 100-101 22.292689768442518 26.863202254068835 27.303346406694867 23.54076157079378 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3599.0 1 2180.5 2 777.5 3 751.0 4 573.5 5 562.0 6 858.5 7 1511.5 8 2314.5 9 2460.0 10 1842.5 11 1324.0 12 1292.5 13 1347.0 14 1439.5 15 1586.5 16 1696.0 17 1819.5 18 2000.5 19 2200.0 20 2413.5 21 2750.0 22 3183.5 23 3794.5 24 4689.0 25 6053.5 26 8455.0 27 12085.5 28 17997.5 29 26835.5 30 38867.5 31 54757.5 32 73090.5 33 90458.5 34 106636.5 35 120748.0 36 132923.5 37 142571.0 38 147587.5 39 151303.5 40 156092.0 41 158167.5 42 158223.0 43 162924.5 44 169687.5 45 174858.5 46 178357.5 47 180257.5 48 180007.0 49 177261.5 50 171731.5 51 163590.5 52 156100.5 53 149456.5 54 139763.5 55 126974.5 56 112990.5 57 99744.5 58 89806.5 59 79141.5 60 65470.0 61 52437.0 62 40820.0 63 30092.0 64 21576.0 65 15374.0 66 11100.5 67 8540.0 68 6751.5 69 5096.0 70 3503.0 71 2377.0 72 1644.5 73 1168.5 74 847.0 75 641.0 76 441.5 77 245.0 78 164.5 79 116.0 80 69.5 81 46.0 82 34.0 83 25.0 84 21.0 85 12.5 86 8.5 87 4.0 88 2.5 89 2.5 90 1.5 91 1.5 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01845954842709076 2 0.0 3 0.0019633016053262793 4 0.017917947984242136 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.385002767803929E-5 12-13 3.385002767803929E-5 14-15 9.026674047477146E-5 16-17 0.0012186009964094146 18-19 1.4668345327150363E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.272169342210465E-4 28-29 0.00547242114128302 30-31 7.898339791542503E-5 32-33 2.5951687886496794E-4 34-35 0.011655692863804865 36-37 0.004603603764213345 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.820835639836608E-4 60-61 0.0017263514115800043 62-63 0.009252340898664075 64-65 0.0020648516883603973 66-67 0.004445636968382494 68-69 0.002944952407989419 70-71 1.3540011071215717E-4 72-73 0.006544338684420931 74-75 0.005596537909435831 76-77 0.0014781178752743826 78-79 4.17483674695818E-4 80-81 0.007289039293337795 82-83 0.01012115827573375 84-85 0.002053568345801051 86-87 0.0016586513562239257 88-89 4.4005035981451086E-4 90-91 2.2566685118692864E-5 92-93 0.0 94-95 0.0 96-97 2.2566685118692864E-5 98-99 2.2566685118692864E-5 100-101 8.011173217135968E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4431311.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.94174337858751 #Duplication Level Percentage of deduplicated Percentage of total 1 54.14649550421979 21.627054282793893 2 19.63069233185871 15.681681509262127 3 9.638300602571896 11.549115878208358 4 5.308871824311686 8.481823841458842 5 3.194200661604029 6.3790971562751295 6 2.0523391976776617 4.91844033356744 7 1.4303970189777475 3.999278546305403 8 1.0116754711214544 3.2326465639955795 9 0.7194420823096787 2.586219392463283 >10 2.7794931563190532 17.513828340031814 >50 0.06729063440287773 1.812460315356711 >100 0.0199547161703592 1.3188304924451015 >500 2.822662685762202E-4 0.08187327709799913 >1k 3.9517253055683225E-4 0.38072972931484717 >5k 1.6935965595292807E-4 0.4369203414233813 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7410 0.16721913672951413 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 6012 0.13567091093358152 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5955 0.13438460988181603 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.2566685118692864E-5 0.0 6 0.0 0.0 0.0 2.2566685118692864E-5 0.0 7 0.0 0.0 0.0 2.2566685118692864E-5 0.0 8 0.0 0.0 0.0 2.2566685118692864E-5 0.0 9 0.0 0.0 0.0 2.2566685118692864E-5 0.0 10-11 0.0 0.0 0.0 2.2566685118692864E-5 0.0 12-13 0.0 0.0 0.0 2.2566685118692864E-5 0.0 14-15 0.0 0.0 0.0 2.2566685118692864E-5 0.0 16-17 0.0 0.0 0.0 2.2566685118692864E-5 0.0 18-19 0.0 0.0 0.0 2.2566685118692864E-5 0.0 20-21 0.0 0.0 0.0 5.641671279673216E-5 0.0 22-23 0.0 0.0 0.0 1.5796679583085006E-4 0.0 24-25 0.0 0.0 0.0 4.0620033213647154E-4 0.0 26-27 0.0 0.0 0.0 8.913840621883682E-4 0.0 28-29 0.0 0.0 0.0 0.002572602103530987 0.0 30-31 0.0 0.0 0.0 0.00613813835228446 0.0 32-33 0.0 0.0 0.0 0.015503312676541998 0.0 34-35 0.0 0.0 0.0 0.030115241290895628 0.0 36-37 0.0 0.0 0.0 0.0496467072611243 0.0 38-39 0.0 0.0 0.0 0.07493267793661966 0.0 40-41 0.0 0.0 0.0 0.10037661540794587 0.0 42-43 0.0 0.0 0.0 0.12957790595153443 1.1283342559346432E-5 44-45 0.0 0.0 0.0 0.1680653874214651 2.2566685118692864E-5 46-47 0.0 0.0 0.0 0.21304079086302 2.2566685118692864E-5 48-49 0.0 0.0 0.0 0.2568540100209622 2.2566685118692864E-5 50-51 0.0 0.0 0.0 0.297169392985507 2.2566685118692864E-5 52-53 0.0 0.0 0.0 0.338466426752715 2.2566685118692864E-5 54-55 0.0 0.0 0.0 0.38831623417990746 2.2566685118692864E-5 56-57 0.0 0.0 0.0 0.45048745168190635 2.2566685118692864E-5 58-59 0.0 0.0 0.0 0.5151071545192834 2.2566685118692864E-5 60-61 0.0 0.0 0.0 0.5792642403117272 2.2566685118692864E-5 62-63 0.0 0.0 0.0 0.6406004904643343 2.2566685118692864E-5 64-65 0.0 0.0 0.0 0.7108844312665032 2.2566685118692864E-5 66-67 0.0 0.0 0.0 0.7992668535338638 2.2566685118692864E-5 68-69 0.0 0.0 0.0 0.9099225940133744 2.2566685118692864E-5 70-71 0.0 0.0 0.0 1.0199239006244427 2.2566685118692864E-5 72-73 0.0 0.0 0.0 1.138297447414546 2.2566685118692864E-5 74-75 0.0 0.0 0.0 1.2674578696913847 2.2566685118692864E-5 76-77 0.0 0.0 0.0 1.410248569779914 2.2566685118692864E-5 78-79 0.0 0.0 0.0 1.5690616163027151 2.2566685118692864E-5 80-81 0.0 0.0 0.0 1.7397221725128298 2.2566685118692864E-5 82-83 0.0 0.0 0.0 1.9263712251295384 2.2566685118692864E-5 84-85 0.0 0.0 0.0 2.1418717846704958 2.2566685118692864E-5 86-87 0.0 0.0 0.0 2.401422513563142 2.2566685118692864E-5 88-89 0.0 2.2566685118692864E-5 0.0 2.686676696805979 2.2566685118692864E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5045 0.0 52.934467 1 GTATCAA 9125 0.0 40.306168 1 TCAACGC 10305 0.0 35.082638 4 ATCAACG 10480 0.0 34.45187 3 CAACGCA 10465 0.0 34.44808 5 TATCAAC 10850 0.0 33.627296 2 AACGCAG 10985 0.0 32.774162 6 ACGCAGA 12565 0.0 28.312733 7 CGCAGAG 12885 0.0 27.425274 8 GCAGAGT 15180 0.0 22.653183 9 GTGGTAT 2715 0.0 22.227842 1 GTACATG 8635 0.0 21.461777 1 CAGAGTA 14130 0.0 20.202246 10-11 GAGTACT 9295 0.0 19.877876 12-13 TACATGG 9075 0.0 19.683472 2 TGGTATC 2865 0.0 19.235073 2 ACATGGG 9010 0.0 19.08729 3 AGAGTAC 13115 0.0 18.705502 10-11 GTACTTT 10265 0.0 17.698837 14-15 CATGGGG 6270 0.0 15.759824 4 >>END_MODULE