FastQCFastQC Report
Fri 10 Feb 2017
SRR2031713_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031713_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565067
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.13662096707116148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6020.10653603908916996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5850.056.0383381
GTATCAA12000.043.1556131
TCAACGC13500.037.6533474
CAACGCA13550.037.1572155
TATCAAC14150.035.5911062
ATCAACG14300.035.5468643
AACGCAG14300.035.205296
CGCAGAG16400.030.7027368
ACGCAGA16600.030.3274527
TATCGCT700.00839324427.1393897
GCAGAGT19250.024.9233079
GTGGTAT3350.024.1099411
ATCGCTC1000.001652130623.7511758
CGTTATC500.001654857423.74696540-41
GTACATG12400.022.2227761
TGGTATC3950.021.6504762
GAGTACA10050.021.2734781
GAGTACT11200.020.14876412-13
TACATGG13700.020.1140482
AGAGTAC15700.019.66744810-11
Produced by FastQC (version 0.11.5)