##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031713_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 565067 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.678712435870438 33.0 31.0 34.0 30.0 34.0 2 31.985398191718858 34.0 31.0 34.0 30.0 34.0 3 32.094804686877836 34.0 31.0 34.0 30.0 34.0 4 35.484057642722014 37.0 35.0 37.0 35.0 37.0 5 35.479847522506184 37.0 37.0 37.0 35.0 37.0 6 35.50176704709353 37.0 37.0 37.0 35.0 37.0 7 35.4833338347488 37.0 37.0 37.0 35.0 37.0 8 35.46316631479099 37.0 37.0 37.0 35.0 37.0 9 37.22386371881564 39.0 38.0 39.0 35.0 39.0 10-11 37.145279232374214 39.0 38.0 39.0 35.0 39.0 12-13 37.090023837881176 39.0 38.0 39.0 35.0 39.0 14-15 38.483572744471005 41.0 39.0 41.0 35.0 41.0 16-17 38.411560045092 41.0 39.0 41.0 34.0 41.0 18-19 38.40126392091557 41.0 39.0 41.0 34.0 41.0 20-21 38.39377542840053 41.0 39.0 41.0 34.0 41.0 22-23 38.287173910350454 41.0 39.0 41.0 34.0 41.0 24-25 38.21819801899598 41.0 39.0 41.0 34.0 41.0 26-27 38.10347180068912 40.0 38.0 41.0 34.0 41.0 28-29 37.99624734765966 40.0 38.0 41.0 34.0 41.0 30-31 37.847849900985196 40.0 38.0 41.0 33.5 41.0 32-33 37.662667435896985 40.0 38.0 41.0 33.0 41.0 34-35 37.509336061033466 40.0 38.0 41.0 33.0 41.0 36-37 37.35508798071733 40.0 38.0 41.0 32.0 41.0 38-39 37.17928670405456 40.0 38.0 41.0 31.5 41.0 40-41 37.09621159968641 40.0 37.5 41.0 31.0 41.0 42-43 37.079020717897166 40.0 38.0 41.0 31.0 41.0 44-45 36.97652844706911 40.0 37.0 41.0 31.0 41.0 46-47 36.94286960661302 40.0 37.5 41.0 31.0 41.0 48-49 37.01972509454632 40.0 38.0 41.0 31.0 41.0 50-51 36.48094739207917 39.5 36.5 40.5 30.5 41.0 52-53 36.50376680995351 39.5 37.0 40.5 31.0 41.0 54-55 36.72504764921682 40.0 37.0 41.0 31.0 41.0 56-57 36.59643369724298 40.0 36.5 41.0 31.0 41.0 58-59 36.41720893274603 40.0 36.0 41.0 30.5 41.0 60-61 36.18182180166246 39.5 35.5 41.0 30.0 41.0 62-63 35.908248048461516 39.0 35.0 41.0 29.5 41.0 64-65 35.644747437029594 39.0 35.0 41.0 29.0 41.0 66-67 35.37268146963103 38.5 35.0 41.0 29.0 41.0 68-69 35.04273210079513 37.5 35.0 40.0 28.5 41.0 70-71 34.690786225350266 37.0 35.0 40.0 28.0 41.0 72-73 34.33235173882035 36.5 35.0 39.0 28.0 41.0 74-75 33.93416355936553 36.0 34.0 39.0 27.0 41.0 76-77 33.55120100802206 35.5 34.0 38.5 26.5 40.0 78-79 33.157383991632855 35.0 34.0 37.0 26.0 39.0 80-81 32.80533635834335 35.0 34.0 37.0 26.0 39.0 82-83 32.476124070242996 35.0 34.0 36.5 25.5 38.5 84-85 32.14715069186486 35.0 34.0 36.0 25.0 37.0 86-87 31.863291432697363 35.0 33.5 36.0 24.5 37.0 88-89 31.59325973734088 35.0 33.0 35.0 23.5 36.5 90-91 31.347960507338065 35.0 33.0 35.0 22.5 36.0 92-93 31.127716713239316 35.0 33.0 35.0 20.0 36.0 94-95 30.88281832065932 35.0 33.0 35.0 18.5 36.0 96-97 30.627340651639543 35.0 32.5 35.0 13.5 35.0 98-99 30.377156160242944 35.0 32.0 35.0 2.0 35.0 100-101 29.783000069018364 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1622088748784627 1101 2 0.08824613076353671 1101 3 0.19983042854812538 1101 4 0.28809917661383366 1101 5 0.22228045646790662 1101 6 0.14919859487790177 1101 7 0.08509634047869241 1101 8 0.27489525663849435 1101 9 0.36240702117671475 1101 10-11 0.29567722023318765 1101 12-13 0.31759515586548304 1101 14-15 0.23815984715731986 1101 16-17 0.37000105823359775 1101 18-19 0.3400314483424012 1101 20-21 0.37794972352151035 1101 22-23 0.4214379364177887 1101 24-25 0.27937213487597035 1101 26-27 0.17013898978377284 1101 28-29 0.015801499430544652 1101 30-31 0.3518693608821266 1101 32-33 -0.04294499129101581 1101 34-35 -0.028334267131889135 1101 36-37 -0.040806419758276036 1101 38-39 0.04686670960060724 1101 40-41 0.1859161384845791 1101 42-43 -0.11718683327220702 1101 44-45 -0.17727283693431417 1101 46-47 -0.34156534124903715 1101 48-49 -0.21021533581554763 1101 50-51 -0.025633117555607043 1101 52-53 -0.09457558486008821 1101 54-55 -0.16354329459507255 1101 56-57 -0.24732348858648123 1101 58-59 -0.3010600417963971 1101 60-61 -0.47366961939682284 1101 62-63 -0.4869415775762178 1101 64-65 -0.2861643041463253 1101 66-67 -0.6054451482394612 1101 68-69 -0.6732699374570643 1101 70-71 -0.865812360794159 1101 72-73 -0.5496132717178099 1101 74-75 -0.49757121669114923 1101 76-77 -0.6341492756316143 1101 78-79 -0.7949397947984949 1101 80-81 -0.9649549246954905 1101 82-83 -0.8127579232692455 1101 84-85 -0.7133890832881953 1101 86-87 -1.1728251746699065 1101 88-89 -1.225879663851586 1101 90-91 -1.7121469473450688 1101 92-93 -1.6285551165291743 1101 94-95 -1.7770493649291872 1101 96-97 -1.8921197501239817 1101 98-99 -2.0722976894279626 1101 100-101 -1.9847074670671567 1102 1 0.14157507029608496 1102 2 0.08350162053974941 1102 3 0.21162872388338627 1102 4 0.31697211051258734 1102 5 0.2324552615790978 1102 6 0.3654493588046961 1102 7 0.293392935347903 1102 8 0.34884803546594156 1102 9 0.43840315838963306 1102 10-11 0.4901811460005021 1102 12-13 0.5137365674622387 1102 14-15 0.6558097051019303 1102 16-17 0.5191102379511676 1102 18-19 0.410149431295757 1102 20-21 0.4857205501486206 1102 22-23 0.6607627884142602 1102 24-25 0.23966426981226618 1102 26-27 0.601793783511873 1102 28-29 0.41424472159211945 1102 30-31 0.24022641676658196 1102 32-33 0.3484071241447424 1102 34-35 0.30182439638110026 1102 36-37 0.3857749670196071 1102 38-39 0.14829519438971772 1102 40-41 0.3533945502632818 1102 42-43 0.6119142289942445 1102 44-45 -0.014942475334521532 1102 46-47 0.08025753969968008 1102 48-49 0.10419127384236049 1102 50-51 0.19886805574264343 1102 52-53 0.23700363259896307 1102 54-55 0.1934122880186493 1102 56-57 0.05892834833672822 1102 58-59 -0.009825489445837832 1102 60-61 -9.797106948141732E-4 1102 62-63 0.0693370842572989 1102 64-65 -0.08199174766239281 1102 66-67 -0.15804063325716555 1102 68-69 -0.224204610636356 1102 70-71 -0.27672799995013264 1102 72-73 -0.15981233587832833 1102 74-75 -0.3601716934747685 1102 76-77 -0.1441631312809335 1102 78-79 -0.4190458255048455 1102 80-81 -0.44588591236535535 1102 82-83 -0.32321030788713756 1102 84-85 -0.31291416574563513 1102 86-87 -0.18714967677624372 1102 88-89 -0.23919181716878057 1102 90-91 -0.5163367650756356 1102 92-93 -0.10059889478280226 1102 94-95 -0.3225397476156928 1102 96-97 -0.6084241638713976 1102 98-99 0.026373661101306567 1102 100-101 -0.06040269876440263 1103 1 0.1002604414635222 1103 2 0.109446144690871 1103 3 0.18448859954096974 1103 4 0.21383166734345593 1103 5 0.34073678557081166 1103 6 0.22961589262689586 1103 7 0.1310304293699076 1103 8 0.3590903437713777 1103 9 0.1362749900491238 1103 10-11 0.29099415413057983 1103 12-13 0.24944914517624994 1103 14-15 0.41116997929290733 1103 16-17 0.3992114654340213 1103 18-19 0.40034550972712424 1103 20-21 0.09297252953115986 1103 22-23 0.24302008157543042 1103 24-25 0.27254991729619604 1103 26-27 0.2194939801216762 1103 28-29 0.12209317262564667 1103 30-31 0.24518815756045598 1103 32-33 0.2059712880216722 1103 34-35 0.1898896495826463 1103 36-37 0.21274505044593894 1103 38-39 0.24214316718317264 1103 40-41 0.41678128707182793 1103 42-43 0.5313307761008943 1103 44-45 0.501908845139468 1103 46-47 0.6118346691964618 1103 48-49 0.6429818578266264 1103 50-51 0.5819250725873175 1103 52-53 0.4186684394431097 1103 54-55 0.22757324237329613 1103 56-57 0.258713140302298 1103 58-59 0.42431686668360413 1103 60-61 0.43775906394782993 1103 62-63 0.508155565579365 1103 64-65 0.32768730010847236 1103 66-67 0.19169234936678237 1103 68-69 0.27552970043225145 1103 70-71 0.3568138991943428 1103 72-73 0.572346652378215 1103 74-75 0.5286839842976931 1103 76-77 0.3372452857444017 1103 78-79 0.2550136356779973 1103 80-81 0.2504117123755947 1103 82-83 0.18989479865412306 1103 84-85 0.22298040888638582 1103 86-87 0.23390085725850795 1103 88-89 0.32601488881984864 1103 90-91 0.4704186416941205 1103 92-93 -0.04910837987765149 1103 94-95 0.3207662197577079 1103 96-97 0.41621184229258645 1103 98-99 0.3421449560691592 1103 100-101 0.36796092310710904 1104 1 -0.40188430391031105 1104 2 -0.26811632725286927 1104 3 -0.20843623461534833 1104 4 -0.27876147944691354 1104 5 -0.30858487635746457 1104 6 -0.43456935352003967 1104 7 -0.3682214036392253 1104 8 -0.3025012568072043 1104 9 -0.282636177590156 1104 10-11 -0.1734180360035893 1104 12-13 -0.24621772100162076 1104 14-15 -0.17299257245531408 1104 16-17 0.06997434637571587 1104 18-19 -0.22235387677594076 1104 20-21 -0.1727327425522418 1104 22-23 -0.16974364728885405 1104 24-25 -0.11787479216562957 1104 26-27 -0.15957729525364783 1104 28-29 -0.2655072064456263 1104 30-31 -0.14880281455784683 1104 32-33 -0.16280022177932096 1104 34-35 -0.23511907903224483 1104 36-37 -0.2774165536240929 1104 38-39 -0.3655118280784393 1104 40-41 0.05191894979815004 1104 42-43 -0.2839717327524127 1104 44-45 -0.24455606582866096 1104 46-47 -0.11292716431144356 1104 48-49 -0.018721967800587436 1104 50-51 -0.35027852858979003 1104 52-53 -0.2751192806426772 1104 54-55 -0.20841483683265238 1104 56-57 -0.31187641528651255 1104 58-59 -0.23840321910621043 1104 60-61 -0.2610476097017269 1104 62-63 -0.3011803115217404 1104 64-65 -0.21043180807647133 1104 66-67 -0.17929248778219886 1104 68-69 -0.01827547688332487 1104 70-71 0.1135910451866664 1104 72-73 -0.01848082031368392 1104 74-75 -0.0996827671612337 1104 76-77 -0.03084663903987206 1104 78-79 -0.1343511705467506 1104 80-81 -0.20257222697300392 1104 82-83 -0.17969346011190623 1104 84-85 -0.10346978813092278 1104 86-87 -0.05226054730032459 1104 88-89 -0.07367462187893992 1104 90-91 0.0771446629420609 1104 92-93 -0.01641348237653162 1104 94-95 0.145589153569869 1104 96-97 0.02537564098578926 1104 98-99 -0.243919186780861 1104 100-101 -0.09580909035989649 1105 1 -0.6218514889581073 1105 2 -0.30717325406125795 1105 3 -0.4393060686382739 1105 4 -0.26674121978076926 1105 5 -0.17254549841482003 1105 6 -0.18454557456917087 1105 7 -0.1982851312275642 1105 8 -0.08248651385766692 1105 9 -0.24205739792591885 1105 10-11 -0.1348188539994979 1105 12-13 -0.29599895465359083 1105 14-15 0.01726281347706049 1105 16-17 -0.27622339937973805 1105 18-19 -0.21884053678449789 1105 20-21 -0.2007502914179753 1105 22-23 -0.1625386251744274 1105 24-25 -0.2597340683343603 1105 26-27 -0.1401439476529518 1105 28-29 -0.384122558136319 1105 30-31 -0.2610275732014955 1105 32-33 -0.0263268779832444 1105 34-35 -0.2032612296005638 1105 36-37 -0.30273519156578743 1105 38-39 -0.3089298144768833 1105 40-41 -0.0970918458455543 1105 42-43 -0.23521299398797169 1105 44-45 -0.04330291511767115 1105 46-47 -0.18125003490639102 1105 48-49 -0.3626666579608582 1105 50-51 -0.3826476254652462 1105 52-53 -0.2177703958754762 1105 54-55 -0.2741993044219413 1105 56-57 -0.3020497638383759 1105 58-59 -0.37789054492539975 1105 60-61 -0.18485390636791976 1105 62-63 -0.3268794126773997 1105 64-65 -0.45599108900099594 1105 66-67 -0.4676241565889754 1105 68-69 -0.23926515681865368 1105 70-71 -0.3641399673210586 1105 72-73 -0.6518450662831583 1105 74-75 -0.42156653564936164 1105 76-77 -0.29652291240269335 1105 78-79 -0.23104232953281922 1105 80-81 -0.3947953210900863 1105 82-83 -0.35985468139882215 1105 84-85 -0.17222662457929871 1105 86-87 -0.42992973453578287 1105 88-89 -0.4683933168901149 1105 90-91 -0.3668142945887318 1105 92-93 -0.3589968275993378 1105 94-95 -0.25812392960890307 1105 96-97 -0.1875648640791283 1105 98-99 -0.13521371286637063 1105 100-101 -0.41058889727481684 1106 1 0.06033012787467129 1106 2 0.24641478580585385 1106 3 0.35885561463968685 1106 4 0.3350860227441501 1106 5 0.22016767987975072 1106 6 0.17562053838764058 1106 7 0.3852696860481828 1106 8 0.26899742855651 1106 9 0.24546198191904267 1106 10-11 0.3425620983814497 1106 12-13 0.3412435702633516 1106 14-15 0.46776115234749227 1106 16-17 0.3334333005095189 1106 18-19 0.17748836686998715 1106 20-21 0.4134080696008482 1106 22-23 0.40065074359633 1106 24-25 0.5108251786202302 1106 26-27 0.46811186630053925 1106 28-29 0.47524067552924265 1106 30-31 0.5047642318926293 1106 32-33 0.687155957011214 1106 34-35 0.553931461429336 1106 36-37 0.6519436567177124 1106 38-39 0.5718121567302106 1106 40-41 0.5741041674434797 1106 42-43 0.5399254334760357 1106 44-45 0.5680702853252981 1106 46-47 0.4508706738422248 1106 48-49 0.7521340064909765 1106 50-51 0.7006358739810494 1106 52-53 0.8174953848554196 1106 54-55 0.7898206291328762 1106 56-57 0.628620225087424 1106 58-59 0.8800718028043875 1106 60-61 0.8641992497782596 1106 62-63 0.9213321044876039 1106 64-65 0.9287209818738589 1106 66-67 0.8736493760799036 1106 68-69 0.9286307457284124 1106 70-71 0.7792819595892411 1106 72-73 0.7896056535396596 1106 74-75 0.8172263768644754 1106 76-77 0.989562358915137 1106 78-79 0.800524669359767 1106 80-81 0.8572700166729206 1106 82-83 1.000400025134951 1106 84-85 0.8296006179456228 1106 86-87 0.6982098003479358 1106 88-89 0.5877860851032395 1106 90-91 0.6636009878032958 1106 92-93 0.5578138036298945 1106 94-95 0.6860565798041662 1106 96-97 0.7044174172054767 1106 98-99 0.8525050025267653 1106 100-101 0.6758064515623978 1107 1 -0.19697145942691563 1107 2 0.07276399215506046 1107 3 0.16179212257619469 1107 4 -0.027215564557437233 1107 5 -0.04800692329484946 1107 6 0.08704910981621339 1107 7 0.01899984477834238 1107 8 -0.0058438412847579 1107 9 0.07752547398253284 1107 10-11 -0.03065218733283359 1107 12-13 0.06497372899351461 1107 14-15 0.004665914252257153 1107 16-17 0.18589361796983894 1107 18-19 0.1810994779810997 1107 20-21 0.04876521245799381 1107 22-23 0.3915634420090299 1107 24-25 0.17467438496944254 1107 26-27 0.2402547234433925 1107 28-29 0.18686765965622953 1107 30-31 0.3923039144323113 1107 32-33 0.3732670681223311 1107 34-35 0.3432965407944195 1107 36-37 0.4860706408446944 1107 38-39 0.4377248551429034 1107 40-41 0.2038263896657071 1107 42-43 0.3056000366506382 1107 44-45 0.3780702853253004 1107 46-47 0.18154527701682355 1107 48-49 0.3300705144274758 1107 50-51 0.33369142953660713 1107 52-53 0.31261443247446863 1107 54-55 0.24601110532334758 1107 56-57 0.22810435207156132 1107 58-59 0.15999243772503036 1107 60-61 0.11011194819095493 1107 62-63 0.2225622632177675 1107 64-65 0.30399875965164114 1107 66-67 0.0997208046513336 1107 68-69 0.20545614255380684 1107 70-71 0.21940100720828326 1107 72-73 0.28793898687298736 1107 74-75 0.28976605940416533 1107 76-77 0.30547505732783975 1107 78-79 0.27108022491531614 1107 80-81 0.3685398579427641 1107 82-83 0.5329793902143223 1107 84-85 0.4016244274694287 1107 86-87 0.37721773685587934 1107 88-89 0.2521908470080021 1107 90-91 0.414354956057263 1107 92-93 0.3557503115664069 1107 94-95 0.4182788020263857 1107 96-97 0.49156027434833405 1107 98-99 0.4638542088759685 1107 100-101 0.49223502299096467 1108 1 -0.08609974937787257 1108 2 0.16279330064871544 1108 3 0.212345646850018 1108 4 0.22315079161330686 1108 5 0.3032633532496476 1108 6 0.33093350086696915 1108 7 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2316 84-85 -0.2970477337027333 2316 86-87 -0.25255943042129303 2316 88-89 -0.10921025189309574 2316 90-91 -0.06630743710513087 2316 92-93 -0.08222282640673129 2316 94-95 -0.22024378649620147 2316 96-97 -0.11915401136594994 2316 98-99 -0.005681810660046693 2316 100-101 -0.13944995869401566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7922.0 3 933.0 4 781.0 5 680.0 6 696.0 7 778.0 8 808.0 9 888.0 10 930.0 11 1050.0 12 1194.0 13 1359.0 14 1490.0 15 1631.0 16 1747.0 17 2054.0 18 2079.0 19 1543.0 20 1546.0 21 1573.0 22 1798.0 23 1954.0 24 2202.0 25 2605.0 26 3232.0 27 3730.0 28 4587.0 29 5538.0 30 6974.0 31 8683.0 32 11484.0 33 15252.0 34 21883.0 35 34100.0 36 59845.0 37 115670.0 38 171465.0 39 62299.0 40 84.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.70651137567919 20.31654834353523 14.060545054353026 23.91639522643255 2 16.111149510233957 21.86818230958318 39.14317969548905 22.877488484693814 3 18.346001371657028 26.062192418316403 30.68010441606221 24.911701793964355 4 13.607637559723818 18.626387648334823 37.85763401528362 29.90834077665773 5 14.18837860012298 36.89779398604695 34.83832783114575 14.075499582684323 6 29.729509474609184 36.9282991056876 19.083861275280977 14.25833014442224 7 25.590612564535963 32.51230479754223 24.002948190512637 17.894134447409172 8 23.9217819098522 34.44717462128392 22.328614477574657 19.30242899128922 9 25.06364185534831 16.92421614669603 22.246216476198743 35.76592552175693 10-11 23.614256374194838 26.403057948514025 29.835544931774482 20.147140745516655 12-13 24.436604060720356 24.871092812289344 28.96591677126581 21.72638635572449 14-15 22.181388401107828 26.899319035983183 26.539256875645655 24.380035687263334 16-17 21.75332298992448 29.1301346740187 27.521648943758382 21.59489339229844 18-19 21.603264119932486 28.35478254705906 28.995999592428024 21.045953740580437 20-21 22.49539301155291 27.61322560068042 28.530104897583104 21.36127649018357 22-23 22.37304292129516 27.45867909534748 28.2415548726199 21.926723110737456 24-25 22.09069344326478 27.62151599810797 28.453796727212815 21.833993831414432 26-27 22.02800238107049 27.809184837683276 28.353075195056444 21.809737586189794 28-29 22.13821401806073 27.663355355589452 28.4854250933244 21.713005533025413 30-31 22.391136054024084 27.561210227302922 28.21383587634126 21.833817842331737 32-33 22.134277421166335 27.76674039031312 28.09278373343986 22.006198455080686 34-35 22.411833440969666 27.887820793922046 27.99439405174163 21.705951713366662 36-37 22.43496949798809 27.678427467579986 28.04900520740128 21.837597827030645 38-39 22.207665665718867 27.77461844401336 28.21308672814081 21.80462916212697 40-41 22.227025341572652 27.679874851182042 28.14714552979194 21.94595427745337 42-43 22.36501783936423 27.609560623722277 27.95607300144203 22.06934853547146 44-45 22.421453155383887 27.774376874249747 27.815571610182634 21.988598360183737 46-47 22.298911671295496 27.82462714664753 27.667999946845974 22.208461235211 48-49 22.298036651418133 27.77032037872364 27.83703825778619 22.094604712072037 50-51 22.305457786250955 27.74490583287783 27.80231238555233 22.147323995318885 52-53 22.196032996652683 27.867432325062914 27.764240280682362 22.172294397602048 54-55 22.403532840948646 27.55461829853604 27.768601043520157 22.273247816995152 56-57 22.227419380557787 27.912372366945366 27.708442960891972 22.151765291604878 58-59 22.122561203538194 27.80799299872978 27.846879102306772 22.222566695425257 60-61 22.33698983478424 27.6720451251962 27.7956114896346 22.19535355038496 62-63 22.161536853517763 27.820747664213663 27.80604381393075 22.211671668337825 64-65 22.33580341416912 27.817203386526895 27.58366202773437 22.263331171569618 66-67 22.17031944862777 27.86653319395475 27.533264825215714 22.429882532201766 68-69 22.23213470982054 27.753823419713726 27.713779726019634 22.30026214444611 70-71 22.2941610003437 27.648614747912113 27.664382420993327 22.39284183075086 72-73 22.217281190288688 27.66689491871953 27.65679631560438 22.4590275753874 74-75 22.150530967964784 27.762051954263246 27.640447447899597 22.446969629872374 76-77 22.23491046520178 27.818908750074193 27.604429210678088 22.341751574045936 78-79 22.2055374976634 27.729037710725855 27.634687808132163 22.43073698347858 80-81 22.25292443770354 27.83176997140235 27.585925844552094 22.329379746342017 82-83 22.207908879725988 27.656183275361347 27.72387140672593 22.41203643818674 84-85 22.272107156145353 27.62521925550575 27.579862156942646 22.522811431406247 86-87 22.157301379875523 27.7105583733859 27.675563135396132 22.456577111342444 88-89 22.31470482193999 27.59728611405656 27.549450286616334 22.53855877738712 90-91 22.255709971287356 27.52356323824762 27.682231382627652 22.538495407837374 92-93 22.343805653954455 27.461089856161387 27.693141926554944 22.50196256332922 94-95 22.12790044628383 27.479585925139826 27.84472724244781 22.547786386128525 96-97 22.1715802022571 27.389381236393522 27.7342705157751 22.704768045574276 98-99 22.114340477620296 27.55900281130261 27.585397523857104 22.74125918722 100-101 22.178536996866715 27.498121446698708 27.59196050075851 22.731381055676067 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 850.0 1 491.0 2 129.0 3 106.5 4 73.5 5 78.0 6 106.5 7 153.5 8 241.0 9 285.0 10 212.0 11 163.0 12 179.5 13 183.0 14 195.0 15 199.0 16 223.5 17 247.5 18 248.0 19 275.0 20 315.5 21 382.5 22 472.5 23 544.0 24 670.0 25 863.5 26 1110.5 27 1553.5 28 2309.0 29 3567.5 30 5583.0 31 8226.0 32 11519.5 33 14904.5 34 17865.5 35 20085.0 36 21525.0 37 22503.0 38 22526.0 39 21939.5 40 21064.0 41 19979.5 42 19406.0 43 19097.0 44 18937.0 45 19189.5 46 19382.0 47 19350.5 48 19257.5 49 19209.0 50 18558.5 51 17827.0 52 17257.5 53 16445.0 54 15722.5 55 14877.5 56 13534.0 57 12039.5 58 10987.5 59 9850.5 60 8308.0 61 6842.5 62 5554.0 63 4293.5 64 3280.5 65 2525.0 66 1940.5 67 1596.0 68 1278.0 69 975.5 70 788.5 71 617.0 72 492.0 73 381.5 74 313.5 75 267.5 76 205.5 77 158.5 78 139.5 79 105.5 80 80.5 81 65.0 82 51.0 83 34.5 84 25.5 85 24.0 86 17.5 87 13.0 88 9.0 89 9.5 90 6.5 91 5.0 92 3.5 93 1.0 94 1.0 95 2.0 96 2.0 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14033734052776042 2 0.14476159464275917 3 0.13909854937556076 4 0.1421070421737599 5 0.13201974279156278 6 0.10812877057056951 7 0.1155615174837674 8 0.10512027777237036 9 0.10264269546797104 10-11 0.08945841820527477 12-13 0.10804028548826952 14-15 0.12741851851196406 16-17 0.13777127314106113 18-19 0.13263913836766258 20-21 0.12511790637216472 22-23 0.1191894058580664 24-25 0.10512027777237036 26-27 0.1088366512289693 28-29 0.11290696501476817 30-31 0.10228875513877117 32-33 0.10113844906887148 34-35 0.11795061470586674 36-37 0.11998577159876617 38-39 0.10653603908916996 40-41 0.10954453188736911 42-43 0.1035275462909708 44-45 0.11936637602266635 46-47 0.11848152519966658 48-49 0.13281610853226256 50-51 0.1188354655288665 52-53 0.10361603137327077 54-55 0.09476752314327327 56-57 0.11609242797756728 58-59 0.10582815843077015 60-61 0.1049433076077704 62-63 0.10423542694937062 64-65 0.1261797273597644 66-67 0.1162693981421672 68-69 0.12095910750406588 70-71 0.109898472216569 72-73 0.11193362910946844 74-75 0.09414812756717346 76-77 0.1198972865164662 78-79 0.12007425668106614 80-81 0.12122456275096583 82-83 0.12635669752436438 84-85 0.1162693981421672 86-87 0.12467548096066486 88-89 0.11264150976786824 90-91 0.12060516717486598 92-93 0.13343550410836236 94-95 0.11113726336876865 96-97 0.09520994855477316 98-99 0.09910329217597207 100-101 0.1421070421737599 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 565067.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.06191499671658 #Duplication Level Percentage of deduplicated Percentage of total 1 70.98420483281205 46.183683009419354 2 18.20937593348116 23.69473738254815 3 6.033055853731328 11.775665013777331 4 2.253826491641281 5.865530704660519 5 1.0168598473191344 3.3079424474925854 6 0.5189021758058329 2.0256461552334226 7 0.2948376407213654 1.3427891062912156 8 0.20063894206054758 1.044316303469959 9 0.11142045373714066 0.6524305280947293 >10 0.3733264901678565 3.611020785369931 >50 0.00191226153869502 0.08086997028703649 >100 0.0010927179890944051 0.17115958724929764 >500 5.463589945469505E-4 0.24420900610641885 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 772 0.13662096707116148 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 602 0.10653603908916996 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.769701645999501E-4 7 0.0 0.0 0.0 0.0 1.769701645999501E-4 8 0.0 0.0 0.0 0.0 1.769701645999501E-4 9 0.0 0.0 0.0 0.0 1.769701645999501E-4 10-11 0.0 0.0 0.0 0.0 3.539403291999002E-4 12-13 0.0 0.0 0.0 0.0 7.078806583998004E-4 14-15 0.0 0.0 0.0 0.0 7.078806583998004E-4 16-17 0.0 0.0 0.0 0.0 7.078806583998004E-4 18-19 0.0 0.0 0.0 0.0 7.078806583998004E-4 20-21 0.0 0.0 0.0 1.769701645999501E-4 7.078806583998004E-4 22-23 0.0 0.0 0.0 5.309104937998503E-4 7.078806583998004E-4 24-25 0.0 0.0 0.0 7.078806583998004E-4 7.078806583998004E-4 26-27 0.0 0.0 0.0 0.0011503060698996757 8.848508229997504E-4 28-29 0.0 0.0 0.0 0.0029200077158991764 8.848508229997504E-4 30-31 0.0 0.0 0.0 0.008494567900797605 8.848508229997504E-4 32-33 0.0 0.0 0.0 0.021678845163493887 8.848508229997504E-4 34-35 0.0 0.0 0.0 0.042738294750887945 8.848508229997504E-4 36-37 0.0 0.0 0.0 0.07114200616917994 0.0010618209875997006 38-39 0.0 0.0 0.0 0.1070669495829698 0.0010618209875997006 40-41 0.0 0.0 0.0 0.14361128857285949 0.0010618209875997006 42-43 0.0 0.0 0.0 0.18289866511404843 0.0010618209875997006 44-45 0.0 0.0 0.0 0.23545880400023358 0.0010618209875997006 46-47 0.0 0.0 0.0 0.29332804782441724 0.0010618209875997006 48-49 0.0 0.0 0.0 0.3526130529654006 0.0012387911521996506 50-51 0.0 0.0 0.0 0.4097744161311845 0.0012387911521996506 52-53 0.0 0.0 0.0 0.4656969881447687 0.0012387911521996506 54-55 0.0 0.0 0.0 0.5337420164334494 0.0012387911521996506 56-57 0.0 0.0 0.0 0.613467075585727 0.0012387911521996506 58-59 0.0 0.0 0.0 0.6946078960548041 0.0012387911521996506 60-61 0.0 0.0 0.0 0.7807923662149798 0.0012387911521996506 62-63 0.0 0.0 0.0 0.8642337988238563 0.0012387911521996506 64-65 0.0 0.0 0.0 0.9623637550945287 0.0012387911521996506 66-67 0.0 0.0 0.0 1.0785446681543958 0.0012387911521996506 68-69 0.0 0.0 0.0 1.2216250462334555 0.0012387911521996506 70-71 0.0 0.0 0.0 1.3562108564117175 0.0012387911521996506 72-73 0.0 0.0 0.0 1.5008839659721769 0.0012387911521996506 74-75 0.0 0.0 0.0 1.66661652512003 0.0012387911521996506 76-77 0.0 0.0 0.0 1.847745488588079 0.0012387911521996506 78-79 0.0 0.0 0.0 2.0429435801418236 0.0014157613167996008 80-81 0.0 0.0 0.0 2.259732031776763 0.0014157613167996008 82-83 0.0 0.0 0.0 2.474131386189602 0.0014157613167996008 84-85 0.0 0.0 0.0 2.7390557225957277 0.0014157613167996008 86-87 0.0 0.0 0.0 3.059991116097737 0.0014157613167996008 88-89 0.0 0.0 0.0 3.3984465558951418 0.0015927314813995509 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 585 0.0 56.038338 1 GTATCAA 1200 0.0 43.155613 1 TCAACGC 1350 0.0 37.653347 4 CAACGCA 1355 0.0 37.157215 5 TATCAAC 1415 0.0 35.591106 2 ATCAACG 1430 0.0 35.546864 3 AACGCAG 1430 0.0 35.20529 6 CGCAGAG 1640 0.0 30.702736 8 ACGCAGA 1660 0.0 30.327452 7 TATCGCT 70 0.008393244 27.139389 7 GCAGAGT 1925 0.0 24.923307 9 GTGGTAT 335 0.0 24.109941 1 ATCGCTC 100 0.0016521306 23.751175 8 CGTTATC 50 0.0016548574 23.746965 40-41 GTACATG 1240 0.0 22.222776 1 TGGTATC 395 0.0 21.650476 2 GAGTACA 1005 0.0 21.273478 1 GAGTACT 1120 0.0 20.148764 12-13 TACATGG 1370 0.0 20.114048 2 AGAGTAC 1570 0.0 19.667448 10-11 >>END_MODULE