FastQCFastQC Report
Fri 10 Feb 2017
SRR2031713_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031713_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565067
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT41780.7393813476985915No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT20590.36438156891129725TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT13390.23696305039933316RNA PCR Primer, Index 11 (95% over 23bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG11480.20316174896074268TruSeq Adapter, Index 11 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9570.16936044752215224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8440.14936281892235787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6800.052.4156841
GTATCAA11400.040.0198331
ATCAACG12600.035.067593
TACCGGG550.002575611734.5534062
TCAACGC12800.034.1484834
CAACGCA13300.032.8559845
AACGCAG13900.031.779456
TATCAAC14250.031.0071332
GTACCGG650.00582745729.245261
ACGCAGA15300.028.561087
CGCAGAG15950.027.3971528
GCAGAGT19100.022.3814079
GTACTAA1302.9291114E-421.9339451
GTGGTAT3700.020.5507221
CAGAGTA17900.020.43223810-11
GAGTACT11650.020.38554612-13
AGAGTAC15700.018.15222410-11
GTACTTT13100.017.94783814-15
CCGTCTT13350.017.78963346-47
TATACAC26350.017.67013
Produced by FastQC (version 0.11.5)