##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031713_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 565067 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.73911589245169 34.0 31.0 34.0 31.0 34.0 2 32.90600229707274 34.0 31.0 34.0 31.0 34.0 3 32.96785513930207 34.0 31.0 34.0 31.0 34.0 4 36.36280476474471 37.0 37.0 37.0 35.0 37.0 5 36.324170408110895 37.0 37.0 37.0 35.0 37.0 6 36.346780116340184 37.0 37.0 37.0 35.0 37.0 7 36.293913819069246 37.0 37.0 37.0 35.0 37.0 8 36.28214707282499 37.0 37.0 37.0 35.0 37.0 9 38.13775003672131 39.0 39.0 39.0 37.0 39.0 10-11 38.09960588744343 39.0 39.0 39.0 36.0 39.0 12-13 38.04520968309953 39.0 39.0 39.0 35.0 39.0 14-15 39.54352492713254 41.0 39.0 41.0 37.0 41.0 16-17 39.51036337283897 41.0 39.0 41.0 37.0 41.0 18-19 39.50366770666133 41.0 39.5 41.0 36.5 41.0 20-21 39.49788166712973 41.0 39.0 41.0 36.5 41.0 22-23 39.4109716192947 41.0 39.0 41.0 36.0 41.0 24-25 39.340046401577155 41.0 39.0 41.0 36.0 41.0 26-27 39.20477837141436 41.0 39.0 41.0 36.0 41.0 28-29 39.09366676871946 40.5 39.0 41.0 36.0 41.0 30-31 38.94203076803282 40.0 38.5 41.0 35.0 41.0 32-33 38.761652158062674 40.0 38.0 41.0 35.0 41.0 34-35 38.62978726416513 40.0 38.0 41.0 35.0 41.0 36-37 38.53351637947358 40.0 38.0 41.0 34.5 41.0 38-39 38.591612145108456 40.0 38.0 41.0 35.0 41.0 40-41 38.547359870599415 40.0 38.0 41.0 35.0 41.0 42-43 38.48118364724891 40.0 38.0 41.0 34.5 41.0 44-45 38.32573305466431 40.0 38.0 41.0 34.0 41.0 46-47 38.14939378870116 40.0 38.0 41.0 33.5 41.0 48-49 37.95641401816068 40.0 38.0 41.0 33.0 41.0 50-51 37.79429076551985 40.0 38.0 41.0 33.0 41.0 52-53 37.875653683545494 40.0 38.0 41.0 33.0 41.0 54-55 37.775283284990984 40.0 37.0 41.0 33.0 41.0 56-57 37.553805123994145 40.0 37.0 41.0 33.0 41.0 58-59 37.34373180525495 40.0 36.5 41.0 33.0 41.0 60-61 37.03978997180865 39.5 36.0 41.0 32.5 41.0 62-63 36.72106316596085 39.0 35.5 41.0 32.0 41.0 64-65 36.343552180537884 39.0 35.0 41.0 31.0 41.0 66-67 35.92172255679415 38.5 35.0 41.0 31.0 41.0 68-69 35.4717307859068 37.5 35.0 40.0 30.0 41.0 70-71 35.12356587802862 37.0 35.0 40.0 30.0 41.0 72-73 34.754684842682366 36.5 35.0 39.0 29.5 41.0 74-75 34.37969125785084 36.0 35.0 39.0 29.0 41.0 76-77 33.45246404408681 35.0 33.5 37.0 27.5 39.0 78-79 33.65809098744043 35.0 34.0 37.0 29.0 39.0 80-81 33.421972084726235 35.0 34.0 37.0 29.0 39.0 82-83 33.13985598168005 35.0 34.0 36.5 29.0 38.5 84-85 32.83610527601152 35.0 34.0 36.0 29.0 37.0 86-87 32.58108065061311 35.0 34.0 36.0 28.0 37.0 88-89 32.34944440216824 35.0 34.0 35.0 27.0 36.5 90-91 32.147485165475956 35.0 34.0 35.0 27.0 36.0 92-93 31.975761281405568 35.0 34.0 35.0 26.5 36.0 94-95 31.79364482441905 35.0 34.0 35.0 26.0 36.0 96-97 31.623165040605805 35.0 33.0 35.0 25.5 35.5 98-99 31.417689406742916 35.0 33.0 35.0 25.0 35.0 100-101 30.71465861570398 34.5 32.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10864001756301178 1101 2 0.14338687769941316 1101 3 0.08795888231788496 1101 4 0.10058132324383706 1101 5 -0.04273213634801465 1101 6 0.15093477682565037 1101 7 0.12551353159290102 1101 8 0.08403235290428768 1101 9 0.13883255137431405 1101 10-11 0.14015493424217595 1101 12-13 0.15344718963127946 1101 14-15 0.08071068007753723 1101 16-17 0.04637474214663939 1101 18-19 0.12080735188622072 1101 20-21 0.0977740606636388 1101 22-23 -4.1519728255678956E-4 1101 24-25 0.12401439036838013 1101 26-27 0.12910177439301407 1101 28-29 0.06887342047500766 1101 30-31 0.15739964017879515 1101 32-33 -0.03492065975193981 1101 34-35 -0.31756458680569466 1101 36-37 -0.09317726887831412 1101 38-39 -0.06651612709502075 1101 40-41 -0.20809773135449916 1101 42-43 -0.19945013390962174 1101 44-45 -0.14230152475654734 1101 46-47 -0.4269149714421445 1101 48-49 -0.5806996345986377 1101 50-51 -0.5162764602018299 1101 52-53 -0.3664135519179226 1101 54-55 -0.20495665871930413 1101 56-57 -0.46480706423885465 1101 58-59 -0.3588685768857047 1101 60-61 -0.37900768740632174 1101 62-63 -0.22707970704006186 1101 64-65 -0.12368664543270569 1101 66-67 -0.5259355010789832 1101 68-69 -0.6187323444995911 1101 70-71 -0.5759213431738814 1101 72-73 -0.8147340761672623 1101 74-75 -0.7819005613017751 1101 76-77 -1.017312384439073 1101 78-79 -0.8515705898718124 1101 80-81 -0.6130790060990918 1101 82-83 -0.5718219002634086 1101 84-85 -0.4626770006533576 1101 86-87 -0.47079480357493964 1101 88-89 -0.49933712381690043 1101 90-91 -0.666765019683595 1101 92-93 -0.8493351687852417 1101 94-95 -0.8022842006653441 1101 96-97 -0.6854716717835672 1101 98-99 -1.013562625744445 1101 100-101 -1.411853044803948 1102 1 0.10211806545597568 1102 2 0.031328730624260004 1102 3 0.06308402635599464 1102 4 -0.07402784947592522 1102 5 -0.1552288123917407 1102 6 -0.05376494746092675 1102 7 0.07862358836840855 1102 8 0.07970771084189465 1102 9 -0.021706372736851165 1102 10-11 0.00940798878304605 1102 12-13 0.08973452874013788 1102 14-15 0.11707359545543738 1102 16-17 0.08041671963237462 1102 18-19 0.21368452178820263 1102 20-21 0.2393909263011622 1102 22-23 0.1412973049917312 1102 24-25 0.08153073023766666 1102 26-27 0.2475909021402316 1102 28-29 0.005652938373053473 1102 30-31 0.04317450687065616 1102 32-33 -0.16893557906727352 1102 34-35 -0.1975312772648934 1102 36-37 -0.20422539976521392 1102 38-39 -0.18478599035795185 1102 40-41 -0.15255850400522064 1102 42-43 -0.11868149526215888 1102 44-45 -0.1929912748416882 1102 46-47 -0.174063366845985 1102 48-49 -0.33254355560743676 1102 50-51 -0.40279076137792913 1102 52-53 -0.38053745034714126 1102 54-55 -0.24305269656341721 1102 56-57 -0.19794819125830543 1102 58-59 -0.45231956660826 1102 60-61 -0.3896723387058145 1102 62-63 -0.3648384507945295 1102 64-65 -0.2737172025044501 1102 66-67 -0.043294716951869816 1102 68-69 -0.03002819760170894 1102 70-71 -0.17447139845652515 1102 72-73 -0.1253338811618292 1102 74-75 -0.1108649331556748 1102 76-77 -0.09265539340092488 1102 78-79 -0.07271981568158026 1102 80-81 -0.18512138479218976 1102 82-83 -0.05137432080336879 1102 84-85 -0.08603659907278427 1102 86-87 -0.05631728284978266 1102 88-89 -0.2538448750496798 1102 90-91 -0.3134436292668532 1102 92-93 -0.3182812530481165 1102 94-95 -0.3574035598532248 1102 96-97 -0.5063045969117432 1102 98-99 -0.624585098021484 1102 100-101 -0.4822392768604189 1103 1 0.12308092214336597 1103 2 0.12760111491672887 1103 3 0.0679992715868778 1103 4 -0.02959137569942527 1103 5 -0.03538981988416623 1103 6 0.08054080736133074 1103 7 -0.01649304907247995 1103 8 0.04252454489258639 1103 9 0.12007852550429021 1103 10-11 0.20883410069122021 1103 12-13 -0.043681168161931794 1103 14-15 0.14327052409139185 1103 16-17 0.23244392617895215 1103 18-19 0.2782602165647532 1103 20-21 0.27433566300343415 1103 22-23 0.21385030993354093 1103 24-25 0.027469674921228204 1103 26-27 0.27946693168484416 1103 28-29 0.3257217518062774 1103 30-31 0.34876991576592786 1103 32-33 0.11404305954263805 1103 34-35 0.0500974490223669 1103 36-37 -0.12677043403908783 1103 38-39 -0.2670632101889723 1103 40-41 -0.07657146962507255 1103 42-43 0.045514264342493505 1103 44-45 -0.10542020305254596 1103 46-47 -0.2843774111629003 1103 48-49 -0.1408091382632719 1103 50-51 -0.14337208875982554 1103 52-53 -0.24457113970364475 1103 54-55 0.02856774022918529 1103 56-57 0.04631957004676224 1103 58-59 0.22493480397465504 1103 60-61 0.19527505488577646 1103 62-63 -0.055895361363631935 1103 64-65 -0.19911839279087218 1103 66-67 0.10622837141627173 1103 68-69 0.12621682073082496 1103 70-71 0.10633523313920534 1103 72-73 0.265076076859323 1103 74-75 0.48521482666152593 1103 76-77 0.29329763279600485 1103 78-79 0.18341095641045513 1103 80-81 0.11943783727061685 1103 82-83 0.26007952902925524 1103 84-85 0.10339296679149612 1103 86-87 0.26532573750198907 1103 88-89 0.2498946966597586 1103 90-91 0.1848958149119042 1103 92-93 0.35827568408987887 1103 94-95 0.4059207400564411 1103 96-97 0.25737838305531113 1103 98-99 0.3045224528579453 1103 100-101 0.21243100317571617 1104 1 0.22465706060413737 1104 2 0.1279377900626244 1104 3 0.15830302738517332 1104 4 0.16274988820401148 1104 5 0.1259773305971521 1104 6 0.15682890153061635 1104 7 0.12231686663404417 1104 8 0.13251656444468551 1104 9 0.0992420748084939 1104 10-11 0.16169958026626574 1104 12-13 0.13130262155326733 1104 14-15 0.16194975996350536 1104 16-17 0.1880526847205246 1104 18-19 0.2156760477782811 1104 20-21 0.2864123251810966 1104 22-23 0.09825227511883838 1104 24-25 0.19456653760764908 1104 26-27 0.2682880698962222 1104 28-29 0.24356678148357247 1104 30-31 0.35281001751663155 1104 32-33 0.37210455886928884 1104 34-35 0.15352530078555304 1104 36-37 0.3000650689785189 1104 38-39 0.05191155164452965 1104 40-41 -0.06976315000813571 1104 42-43 -0.048530326410158864 1104 44-45 0.015383827073648604 1104 46-47 -0.16267558064765808 1104 48-49 -0.41911478941793945 1104 50-51 -0.2969458330862338 1104 52-53 -0.08532379569646054 1104 54-55 -0.2865789005010271 1104 56-57 -0.16387171131390943 1104 58-59 -0.2298634403268025 1104 60-61 -0.15209264425134705 1104 62-63 -0.13068712155116913 1104 64-65 -0.011210018308354108 1104 66-67 -0.07570713658912354 1104 68-69 -0.2863536316608162 1104 70-71 -0.18477654300923518 1104 72-73 -0.34836474313170385 1104 74-75 -0.19169549305517108 1104 76-77 -0.1418487585452084 1104 78-79 0.1548621509837531 1104 80-81 -0.04700437929878376 1104 82-83 -0.023887252356352917 1104 84-85 0.09169038810927077 1104 86-87 0.007632086945854155 1104 88-89 0.022395818910247556 1104 90-91 -0.01769533676648649 1104 92-93 -0.020240700767214292 1104 94-95 0.12987205932423151 1104 96-97 0.010021906423059335 1104 98-99 -0.05929245062851152 1104 100-101 0.10546806238066608 1105 1 0.027410663115190914 1105 2 -0.07262577107306356 1105 3 -0.048799745240501124 1105 4 -0.06920188808361871 1105 5 -0.002964030509993165 1105 6 0.011860527535276333 1105 7 0.031909295416085115 1105 8 0.03080210208675993 1105 9 -0.06303737542216226 1105 10-11 0.009008991975022695 1105 12-13 0.06477506174920222 1105 14-15 -0.07780151221036391 1105 16-17 0.03216977227462081 1105 18-19 -0.11063655030989139 1105 20-21 -0.15152461304118958 1105 22-23 -0.07545296100614252 1105 24-25 0.004828343856750905 1105 26-27 0.05801675206676293 1105 28-29 0.08229199192728487 1105 30-31 0.04197751644657899 1105 32-33 -0.16800458651521666 1105 34-35 -0.277634636438016 1105 36-37 -0.04591866621908025 1105 38-39 0.060787045057765 1105 40-41 0.029757704131782248 1105 42-43 0.07148798338201345 1105 44-45 0.008755444517888122 1105 46-47 -0.12565175413888596 1105 48-49 -0.279894976796065 1105 50-51 -0.26589052324650453 1105 52-53 -0.20091922062631085 1105 54-55 -0.2585375726844106 1105 56-57 -0.14308296351346428 1105 58-59 -0.0877188167475964 1105 60-61 -0.031658202818896086 1105 62-63 -0.08286170660847603 1105 64-65 -0.3029896349916612 1105 66-67 -0.4834388624653698 1105 68-69 -0.4048381991216985 1105 70-71 -0.3591119213323424 1105 72-73 -0.46984545840255265 1105 74-75 -0.5970077106801241 1105 76-77 -0.7448948421520001 1105 78-79 -0.5284438365560788 1105 80-81 -0.7843955905985425 1105 82-83 -0.6674823919387975 1105 84-85 -0.6208696537416856 1105 86-87 -0.5852603846382998 1105 88-89 -0.770501408464078 1105 90-91 -0.7152270816659012 1105 92-93 -0.6247551345903979 1105 94-95 -0.6485318974931893 1105 96-97 -0.6899887941048313 1105 98-99 -0.8723304493920061 1105 100-101 -0.9514468565331136 1106 1 0.16617035303767125 1106 2 0.1451040185171948 1106 3 0.1675346955125434 1106 4 0.08354451280231956 1106 5 0.18366941356531186 1106 6 0.04558145776783107 1106 7 0.13463354790778226 1106 8 0.16049202456738243 1106 9 0.12213427463320414 1106 10-11 0.188903787102376 1106 12-13 0.03110396540368754 1106 14-15 0.22903126741311297 1106 16-17 0.29107342675967374 1106 18-19 0.2747178239979746 1106 20-21 0.19631591852026986 1106 22-23 0.16680617520648156 1106 24-25 0.18115724751123707 1106 26-27 -0.016473834865685433 1106 28-29 0.13698191440790453 1106 30-31 0.37594761611435956 1106 32-33 0.34525676453682763 1106 34-35 0.3351671354335153 1106 36-37 0.2171378121862375 1106 38-39 0.16290775380637967 1106 40-41 0.18033909948061932 1106 42-43 0.3252587475016142 1106 44-45 0.2473324101878731 1106 46-47 0.22298611961527115 1106 48-49 0.3906310475671617 1106 50-51 0.5386720459672318 1106 52-53 0.5828736918875279 1106 54-55 0.6098511316345281 1106 56-57 0.6894031937401337 1106 58-59 0.5846676727319178 1106 60-61 0.7009719189972756 1106 62-63 0.6313520253959481 1106 64-65 0.6318165665587259 1106 66-67 0.8055257001038356 1106 68-69 0.6932957986634563 1106 70-71 0.7385071262867058 1106 72-73 0.38926306872923533 1106 74-75 0.5768350357207552 1106 76-77 0.5497784690329368 1106 78-79 0.633056717153778 1106 80-81 0.39428104284553456 1106 82-83 0.3561887044067191 1106 84-85 0.4585323396137966 1106 86-87 0.3351050638666848 1106 88-89 0.4617355793543041 1106 90-91 0.6786821099177089 1106 92-93 0.6926368919876786 1106 94-95 0.8056652232155521 1106 96-97 0.6745793122074595 1106 98-99 0.5663517211506317 1106 100-101 0.5635808289596866 1107 1 0.19513860700593 1107 2 0.10248497089814634 1107 3 0.19174104471889564 1107 4 0.017592131849937687 1107 5 0.09827258816848428 1107 6 0.06423225141862332 1107 7 0.12733196060619179 1107 8 0.12382535790071358 1107 9 0.1374517349506661 1107 10-11 0.15763394583252932 1107 12-13 0.15320714000686309 1107 14-15 0.3088725372543806 1107 16-17 0.18746231564855975 1107 18-19 0.09356703034718095 1107 20-21 0.22449052169487516 1107 22-23 0.3102982386985431 1107 24-25 0.17361756497155767 1107 26-27 0.2454706095787671 1107 28-29 0.37372794615394156 1107 30-31 0.2695984097651518 1107 32-33 0.19089168517174926 1107 34-35 0.09810364337003108 1107 36-37 0.09467749472591436 1107 38-39 0.1011617220603469 1107 40-41 0.14799782963935115 1107 42-43 0.1247825570254193 1107 44-45 0.24221336256882608 1107 46-47 0.4536210402501837 1107 48-49 0.5553532697893857 1107 50-51 0.5558149031100825 1107 52-53 0.6066435331573743 1107 54-55 0.5929066871900872 1107 56-57 0.5495619238988638 1107 58-59 0.2251041806684242 1107 60-61 0.27156715709250534 1107 62-63 0.4119472634911929 1107 64-65 0.4138800586222189 1107 66-67 0.37877966835780796 1107 68-69 0.5041688145364773 1107 70-71 0.4900944278740127 1107 72-73 0.47319164015780757 1107 74-75 0.3325096388953668 1107 76-77 0.1765245007789744 1107 78-79 0.5134535425506073 1107 80-81 0.6145985031629948 1107 82-83 0.44150616472418136 1107 84-85 0.41666726024872247 1107 86-87 0.372565381327 1107 88-89 0.40860065871938644 1107 90-91 0.3937614749970706 1107 92-93 0.3252956221464025 1107 94-95 0.22923665178698727 1107 96-97 0.4177539153820611 1107 98-99 0.29087553067444105 1107 100-101 0.4102871781660369 1108 1 -0.010307404427287281 1108 2 0.0014250771051607103 1108 3 0.024294510956089255 1108 4 0.09775884204406537 1108 5 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2316 76-77 -0.3355328862784148 2316 78-79 -0.06185170775464144 2316 80-81 0.00464734321183613 2316 82-83 0.13640848462650723 2316 84-85 0.20578508686654828 2316 86-87 0.29439077815239756 2316 88-89 0.1969553595740834 2316 90-91 0.07840738464297914 2316 92-93 0.4173438516946426 2316 94-95 0.3582659558162895 2316 96-97 0.1643045869327331 2316 98-99 0.24160813140704107 2316 100-101 0.6640570194358801 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 3.0 6 8.0 7 31.0 8 101.0 9 160.0 10 337.0 11 473.0 12 637.0 13 851.0 14 1092.0 15 1228.0 16 1679.0 17 2254.0 18 2334.0 19 1775.0 20 1643.0 21 2027.0 22 2521.0 23 2706.0 24 3456.0 25 3817.0 26 3506.0 27 3095.0 28 3857.0 29 4816.0 30 6334.0 31 8008.0 32 10612.0 33 13947.0 34 20209.0 35 31723.0 36 56713.0 37 117213.0 38 188138.0 39 67696.0 40 65.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.42813802087943 20.809511503509995 14.488333646040944 24.274016829569632 2 15.578506619569007 21.896518465951825 39.67423332100441 22.850741593474755 3 17.867160774361615 26.27407049529341 31.694389435439486 24.164379294905487 4 13.18517154953909 19.454553178233102 37.23821526174934 30.122060010478467 5 13.555206727697778 37.10321076969634 35.48287194261919 13.858710559986692 6 29.180256500556574 37.486528146219825 18.986244109105645 14.346971244117954 7 24.906603995632377 32.75328412382956 23.686040770386523 18.65407111015154 8 23.598971449403344 34.7456142368958 22.711820014263793 18.94359429943706 9 24.413388146892316 16.602455991944318 21.88554631574663 37.098609545416736 10-11 23.0554960739169 26.46217174246594 30.109703805035508 20.372628378581656 12-13 24.006091313065532 24.820596495636803 29.098230829264494 22.075081362033174 14-15 21.843977655727244 26.782069013116157 26.39547734647161 24.97847598468499 16-17 21.705413323729626 29.29602988711808 27.315618974638955 21.68293781451334 18-19 21.532332920107635 28.009712131227033 28.814484666848944 21.643470281816388 20-21 22.036767321397285 27.490810824203148 28.47166796149837 22.000753892901194 22-23 21.90421666811192 27.94677445329492 28.12401007314177 22.02499880545139 24-25 21.980313838889902 27.995087308230705 28.405923545349488 21.6186753075299 26-27 21.913565772463546 27.995781020076432 28.738084345974052 21.352568861485967 28-29 21.315798322599598 27.40128592438425 29.174166122458374 22.108749630557785 30-31 22.151443988058052 27.211905844793627 29.04726342185971 21.58938674528861 32-33 21.648817703434382 27.89862414180303 28.566599801261976 21.885958353500612 34-35 21.69534063183903 27.943111655455784 28.962135498755316 21.399412213949876 36-37 22.261629182860094 27.630680088630267 28.336111878013035 21.7715788504966 38-39 21.819713414515444 27.251989587075514 28.910200029377048 22.01809696903199 40-41 21.793521830154656 27.219073136459926 28.983642647686025 22.003762385699392 42-43 22.215064762231734 26.988392526903887 29.020098501593615 21.77644420927076 44-45 21.796176382623653 27.47806897235195 28.750219000578692 21.975535644445703 46-47 22.3375281161349 27.019539275873484 28.305935402350517 22.3369972056411 48-49 22.08720381830827 27.117934687391056 28.43530059267308 22.359560901627596 50-51 21.816262496305747 27.47913079333955 28.55068513999225 22.153921570362453 52-53 21.81714734712875 27.561244949713924 28.873655690387157 21.747952012770167 54-55 21.88687359198113 27.55186553099013 28.270010458936728 22.291250418092016 56-57 22.219223561099835 28.004024301543 27.69149499085949 22.08525714649767 58-59 21.46744179873112 28.371514781485317 28.49265128790493 21.668392131878633 60-61 22.051315177052935 28.620740916403125 27.698090635104066 21.629853271439874 62-63 21.59993840876932 28.96460362591823 27.75118558694408 21.68427237836838 64-65 21.631307339449542 29.04278513987994 27.524351568694076 21.801555951976443 66-67 21.70741700019379 28.81798223686015 27.552511165010312 21.922089597935745 68-69 21.660561012299798 28.639500929121315 27.71542341385718 21.984514644721706 70-71 21.697130686619516 28.519525612517487 27.63343364618792 22.14991005467508 72-73 21.784272204293035 28.231700356528794 27.74668314952352 22.237344289654647 74-75 21.827201396023423 28.1244386394789 27.675615693582774 22.37274427091491 76-77 21.763664341530447 28.169749396741743 27.716962816992634 22.349623444735172 78-79 21.810646060133067 27.99986196241661 27.78908919086085 22.40040278658947 80-81 21.804732837667316 27.9013894812851 27.73219522468679 22.561682456360803 82-83 21.90680482143505 28.046515291092238 27.696349765538653 22.35033012193406 84-85 21.936105561031898 27.836395266318263 27.66048380959797 22.56701536305187 86-87 21.78236570405162 27.731224051336323 27.86156465353999 22.624845591072066 88-89 21.95375753231929 27.615761018643877 27.713625866050805 22.716855582986028 90-91 21.884838435088227 27.643447591170606 27.736976323161677 22.73473765057949 92-93 21.84325044640724 27.446568283053157 27.928192586011924 22.781988684527676 94-95 21.723707100219976 27.52903637975674 27.860678468217042 22.886578051806246 96-97 21.816174011223445 27.436569468753262 27.890055515540634 22.857201004482654 98-99 21.812900063178347 27.385071150854678 27.671408877177395 23.130619908789576 100-101 21.69633013072925 27.326784187901836 27.846728052369567 23.13015762899935 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 394.0 1 244.0 2 92.5 3 82.0 4 61.5 5 63.0 6 89.0 7 169.5 8 260.0 9 279.0 10 234.0 11 173.0 12 147.0 13 154.0 14 192.0 15 216.5 16 233.5 17 252.5 18 265.0 19 306.0 20 345.5 21 399.0 22 477.5 23 551.5 24 663.5 25 849.5 26 1166.5 27 1712.5 28 2477.0 29 3755.0 30 6052.0 31 8726.5 32 11838.5 33 15064.0 34 17850.5 35 20187.5 36 21847.5 37 22832.5 38 22845.5 39 22404.5 40 21623.5 41 20837.5 42 20494.0 43 20356.5 44 20254.5 45 20270.0 46 20196.5 47 19869.0 48 19661.0 49 19292.5 50 18502.0 51 17485.0 52 16540.5 53 15935.5 54 15050.0 55 13850.0 56 12735.0 57 11334.0 58 10207.0 59 9132.5 60 7530.5 61 6177.0 62 5140.0 63 3987.0 64 2937.5 65 2216.5 66 1711.0 67 1360.5 68 1063.0 69 792.0 70 634.0 71 492.5 72 372.5 73 296.5 74 219.5 75 186.0 76 139.5 77 96.0 78 86.5 79 67.5 80 46.5 81 41.5 82 28.5 83 20.5 84 13.0 85 9.5 86 8.5 87 4.0 88 2.5 89 1.0 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019289747941394562 2 0.0 3 0.0014157613167996006 4 0.01752004629539506 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.848508229997504E-5 16-17 0.0013272762344996257 18-19 8.848508229997504E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.6545524689992515E-4 28-29 0.004601224279598702 30-31 0.0 32-33 2.6545524689992515E-4 34-35 0.01159154578129673 36-37 0.004070313785798852 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.5394032919990016E-4 60-61 0.001769701645999501 62-63 0.00911396347689743 64-65 0.0019466718105994511 66-67 0.0036278883742989773 68-69 0.003008492798199152 70-71 2.6545524689992515E-4 72-73 0.006282440843298228 74-75 0.005928500514098328 76-77 0.001858186728299476 78-79 6.193955760998254E-4 80-81 0.0063709259255982035 82-83 0.00858305298309758 84-85 0.001769701645999501 86-87 0.0015927314813995509 88-89 3.5394032919990016E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0010618209875997006 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 565067.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.90139261708277 #Duplication Level Percentage of deduplicated Percentage of total 1 68.90911963573838 42.655704694693696 2 19.333683503909445 23.935638666198304 3 6.474658969391238 12.023712207780104 4 2.51302211983452 6.22238275581161 5 1.0967330500739578 3.394465156437939 6 0.5589513818308512 2.075992136434348 7 0.3466911361927615 1.502246489683145 8 0.19520039369212697 0.9666540967156384 9 0.1320436540126106 0.735631746266596 >10 0.429856791971686 3.881032396981219 >50 0.004876265858205519 0.18732306708943858 >100 0.0031552262464857165 0.4549014967415772 >500 8.605162490384986E-4 0.4153062454632525 >1k 0.0011473549987179982 1.5490088437030418 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 4178 0.7393813476985915 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 2059 0.36438156891129725 TruSeq Adapter, Index 11 (95% over 21bp) ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 1339 0.23696305039933316 RNA PCR Primer, Index 11 (95% over 23bp) TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 1148 0.20316174896074268 TruSeq Adapter, Index 11 (96% over 28bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 957 0.16936044752215224 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 844 0.14936281892235787 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.769701645999501E-4 0.0 22-23 0.0 0.0 0.0 5.309104937998503E-4 1.769701645999501E-4 24-25 0.0 0.0 0.0 7.078806583998004E-4 1.769701645999501E-4 26-27 0.0 0.0 0.0 0.0011503060698996757 3.539403291999002E-4 28-29 0.0 0.0 0.0 0.0030969778804991266 3.539403291999002E-4 30-31 0.0 0.0 0.0 0.008848508229997505 3.539403291999002E-4 32-33 0.0 0.0 0.0 0.022298240739593713 3.539403291999002E-4 34-35 0.0 0.0 0.0 0.04415405606768755 3.539403291999002E-4 36-37 0.0 0.0 0.0 0.07273473765057949 3.539403291999002E-4 38-39 0.0 0.0 0.0 0.10830574073516946 3.539403291999002E-4 40-41 0.0 0.0 0.0 0.14476159464275917 3.539403291999002E-4 42-43 0.0 0.0 0.0 0.18422594134854806 3.539403291999002E-4 44-45 0.0 0.0 0.0 0.23625516974093339 3.539403291999002E-4 46-47 0.0 0.0 0.0 0.29483229422351687 3.539403291999002E-4 48-49 0.0 0.0 0.0 0.3542057844468001 3.539403291999002E-4 50-51 0.0 0.0 0.0 0.41048229678958426 3.539403291999002E-4 52-53 0.0 0.0 0.0 0.4662278986385685 3.539403291999002E-4 54-55 0.0 0.0 0.0 0.5348038374210492 4.424254114998753E-4 56-57 0.0 0.0 0.0 0.613290105421127 5.309104937998503E-4 58-59 0.0 0.0 0.0 0.6946078960548041 5.309104937998503E-4 60-61 0.0 0.0 0.0 0.78026145572118 5.309104937998503E-4 62-63 0.0 0.0 0.0 0.865295619811456 5.309104937998503E-4 64-65 0.0 0.0 0.0 0.9640449716582281 5.309104937998503E-4 66-67 0.0 0.0 0.0 1.081730131117195 5.309104937998503E-4 68-69 0.0 0.0 0.0 1.2256068749369544 5.309104937998503E-4 70-71 0.0 0.0 0.0 1.3593963193745167 5.309104937998503E-4 72-73 0.0 0.0 0.0 1.5046003394287757 7.078806583998004E-4 74-75 0.0 0.0 0.0 1.6708638090704286 7.078806583998004E-4 76-77 0.0 0.0 0.0 1.8534970189375772 7.078806583998004E-4 78-79 0.0 0.0 0.0 2.0505532972196217 7.078806583998004E-4 80-81 0.0 0.0 0.0 2.26902296541826 7.078806583998004E-4 82-83 0.0 0.0 0.0 2.4829798944196 7.078806583998004E-4 84-85 0.0 0.0 0.0 2.7489660518133245 7.078806583998004E-4 86-87 0.0 0.0 0.0 3.072025087290534 7.078806583998004E-4 88-89 0.0 0.0 0.0 3.4103920420056384 7.078806583998004E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 680 0.0 52.415684 1 GTATCAA 1140 0.0 40.019833 1 ATCAACG 1260 0.0 35.06759 3 TACCGGG 55 0.0025756117 34.553406 2 TCAACGC 1280 0.0 34.148483 4 CAACGCA 1330 0.0 32.855984 5 AACGCAG 1390 0.0 31.77945 6 TATCAAC 1425 0.0 31.007133 2 GTACCGG 65 0.005827457 29.24526 1 ACGCAGA 1530 0.0 28.56108 7 CGCAGAG 1595 0.0 27.397152 8 GCAGAGT 1910 0.0 22.381407 9 GTACTAA 130 2.9291114E-4 21.933945 1 GTGGTAT 370 0.0 20.550722 1 CAGAGTA 1790 0.0 20.432238 10-11 GAGTACT 1165 0.0 20.385546 12-13 AGAGTAC 1570 0.0 18.152224 10-11 GTACTTT 1310 0.0 17.947838 14-15 CCGTCTT 1335 0.0 17.789633 46-47 TATACAC 2635 0.0 17.6701 3 >>END_MODULE