FastQCFastQC Report
Fri 10 Feb 2017
SRR2031712_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031712_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6662582
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57350.043.4083441
TCAACGC89200.030.942634
GTATCAA94250.030.7484911
CAACGCA92100.029.811795
ATCAACG92800.029.7936863
AACGCAG96700.028.3889586
TATCAAC106550.026.3963322
ACGCAGA108950.024.8486187
CGCAGAG111000.024.0918068
GTACATG115250.019.9929161
GCAGAGT147450.017.5886239
CAGAGTA140400.016.20749910-11
TACATGG135200.015.9885852
ACATGGG123000.015.8739843
GTGGTAT43100.014.77076151
ATACCGT7150.014.6138526
CATGGGG72100.014.4955064
AGAGTAC123200.014.36359510-11
GAGTACT65800.014.11576312-13
GTATAGG26600.012.5023531
Produced by FastQC (version 0.11.5)