FastQCFastQC Report
Fri 10 Feb 2017
SRR2031712_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031712_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6662582
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT79400.11917301730770442No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56850.039.9496881
TCAACGC90600.034.9737244
GTATCAA97150.034.3328821
CAACGCA94650.033.470695
ATCAACG96050.032.989273
AACGCAG100950.031.4289326
TATCAAC109150.029.5957552
ACGCAGA113350.027.739337
CGCAGAG113750.027.516528
GCAGAGT150200.020.1116039
GTACATG115650.018.7342011
CAGAGTA145900.016.89577310-11
AGAGTAC126600.016.33881210-11
ACATGGG118850.016.0283743
GAGTACT74950.015.24085912-13
TACATGG132800.015.2389762
CATGGGG66750.015.0879024
GTGGTAT45750.014.8511771
GGACCGT5804.3473847E-1013.1024186
TAGGGCG3306.30239E-512.9535275
Produced by FastQC (version 0.11.5)