FastQCFastQC Report
Fri 10 Feb 2017
SRR2031710_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031710_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11418947
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133080.11654314535306978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120550.049.41851
GTATCAA217000.038.7063141
TCAACGC235100.034.936914
ATCAACG242900.033.8345763
CAACGCA248100.033.124125
AACGCAG255200.032.3448076
TATCAAC267900.030.9621262
ACGCAGA291250.028.1954987
CGCAGAG300550.027.1845078
GCAGAGT356950.022.2105189
GTACATG256550.021.4064711
GTGGTAT73300.019.962091
ACATGGG261450.019.6417033
GAGTACT177950.019.52888712-13
CAGAGTA337800.019.21021310-11
TACATGG286500.018.6546542
AGAGTAC300050.018.2785410-11
CATGGGG153700.016.9065724
GTACTTT216950.015.78848914-15
TGGTATC93700.014.2977862
Produced by FastQC (version 0.11.5)