FastQCFastQC Report
Fri 10 Feb 2017
SRR2031710_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031710_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11418947
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178540.1563541717112795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143700.12584347751154287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130750.052.3308831
GTATCAA236900.039.5930821
TCAACGC261950.035.093264
ATCAACG269700.034.0325853
CAACGCA272700.033.684185
AACGCAG278950.032.9975786
TATCAAC290650.031.8247052
ACGCAGA314650.028.9670187
CGCAGAG323850.028.0414528
GCAGAGT378850.023.3812719
GTACATG246750.022.5302491
GAGTACT222450.020.56210312-13
ACATGGG249500.020.4504323
CAGAGTA362400.020.24293310-11
GTGGTAT77100.020.2142071
TACATGG272850.019.4838562
AGAGTAC327650.019.12814510-11
CATGGGG143600.017.699484
GTACTTT263650.017.10562714-15
AGTACTT274300.015.2813812-13
Produced by FastQC (version 0.11.5)