FastQCFastQC Report
Fri 10 Feb 2017
SRR2031708_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031708_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260978
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19240.7372268926882726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17750.6801339576516029No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10180.39007119374046856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8590.3291465180973109No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.23182030669251816No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5670.21725969238786413No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG250.005016933757.05
GGTATCA11100.047.927931
CGGAGAT405.3804705E-447.5000041
GTATCAA18900.037.6984141
TGGTATC5950.034.327732
GTGGTAT6450.032.4031031
TATCAAC23900.029.4142252
GCGTCAG650.005835896429.2307661
ATCAACG24300.028.9300423
TCAACGC24450.028.7525564
CAACGCA25100.028.197215
GGATAGA1205.360769E-627.7083341
AACGCAG25800.027.4321736
CGCGATA350.00838743927.14285944-45
GTACTGG700.00837988127.1428591
CGCAGAG27300.025.9249068
ACGCAGA27300.025.7509147
GCAGAGT28700.024.329279
CTTACAC1652.4179262E-623.0303023
GAGTACT13950.022.30286612-13
Produced by FastQC (version 0.11.5)