##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031708_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 260978 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71261945451341 33.0 31.0 34.0 30.0 34.0 2 32.00367080750102 34.0 31.0 34.0 31.0 34.0 3 32.060836545609206 34.0 31.0 34.0 30.0 34.0 4 35.47926261983769 37.0 35.0 37.0 35.0 37.0 5 35.484607897983736 37.0 35.0 37.0 35.0 37.0 6 35.529213190383864 37.0 37.0 37.0 35.0 37.0 7 35.530745886626455 37.0 37.0 37.0 35.0 37.0 8 35.517254327951015 37.0 37.0 37.0 35.0 37.0 9 37.266673819249135 39.0 38.0 39.0 35.0 39.0 10-11 37.207283755718876 39.0 38.0 39.0 35.0 39.0 12-13 37.15554759405008 39.0 38.0 39.0 35.0 39.0 14-15 38.44617362383036 41.0 39.0 41.0 34.5 41.0 16-17 38.36826667381925 41.0 38.5 41.0 34.5 41.0 18-19 38.44595713048609 41.0 39.0 41.0 35.0 41.0 20-21 38.49171002919786 41.0 39.0 41.0 35.0 41.0 22-23 38.381183471403716 41.0 39.0 41.0 35.0 41.0 24-25 38.31676041658684 41.0 39.0 41.0 34.0 41.0 26-27 38.18942401275203 40.5 38.0 41.0 34.0 41.0 28-29 38.0956766470737 40.0 38.0 41.0 34.0 41.0 30-31 37.8837718121834 40.0 38.0 41.0 34.0 41.0 32-33 37.61234088697132 40.0 38.0 41.0 33.5 41.0 34-35 37.42707622864762 40.0 37.5 41.0 33.0 41.0 36-37 37.26164082796251 40.0 37.0 41.0 33.0 41.0 38-39 37.06032309236794 40.0 37.0 41.0 32.5 41.0 40-41 36.94288407451969 40.0 37.0 41.0 32.0 41.0 42-43 36.83656093617087 40.0 37.0 41.0 31.0 41.0 44-45 36.591431078481705 40.0 37.0 41.0 30.5 41.0 46-47 36.30327652139261 40.0 37.0 41.0 30.0 41.0 48-49 36.141837626160054 40.0 37.0 41.0 28.5 41.0 50-51 35.5567576577336 39.5 36.5 40.5 26.5 41.0 52-53 35.56242097034999 39.5 36.5 40.5 26.0 41.0 54-55 35.77957720574148 40.0 37.0 41.0 26.0 41.0 56-57 35.676765857658495 40.0 36.5 41.0 26.0 41.0 58-59 35.51406823563672 40.0 36.0 41.0 25.0 41.0 60-61 35.32172826828315 40.0 35.5 41.0 24.0 41.0 62-63 35.05542612787285 39.0 35.0 41.0 23.0 41.0 64-65 34.8090605338381 39.0 35.0 41.0 22.5 41.0 66-67 34.53659695453257 39.0 35.0 41.0 22.0 41.0 68-69 34.200677834913286 38.0 35.0 40.5 20.5 41.0 70-71 33.8406608986198 37.0 35.0 40.0 19.5 41.0 72-73 33.46665619324234 37.0 35.0 39.0 18.0 41.0 74-75 33.038282920399425 36.0 34.0 39.0 14.0 41.0 76-77 32.64078581336358 36.0 34.0 38.5 10.5 40.0 78-79 32.24687713140571 35.5 34.0 37.0 7.5 39.0 80-81 31.868965966479934 35.0 34.0 37.0 4.5 39.0 82-83 31.534665757266897 35.0 34.0 36.5 2.0 39.0 84-85 31.198714067852464 35.0 33.5 36.0 2.0 37.0 86-87 30.92996152932431 35.0 33.0 36.0 2.0 37.0 88-89 30.660500118783958 35.0 33.0 35.5 2.0 36.5 90-91 30.42518909639893 35.0 33.0 35.0 2.0 36.0 92-93 30.209052104008766 35.0 33.0 35.0 2.0 36.0 94-95 29.977933005847238 35.0 32.0 35.0 2.0 36.0 96-97 29.754699629853857 35.0 32.0 35.0 2.0 36.0 98-99 29.541137567151253 35.0 32.0 35.0 2.0 35.0 100-101 29.02704250933029 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24003278472249434 1101 2 0.392908087492458 1101 3 0.6249122308946653 1101 4 0.6424393766709642 1101 5 0.6189775522679639 1101 6 0.8784001905763645 1101 7 0.647924978064502 1101 8 0.576020046282224 1101 9 0.6044734178121303 1101 10-11 0.432892665699967 1101 12-13 0.5520296200547605 1101 14-15 0.6814327302730518 1101 16-17 0.8444856574336157 1101 18-19 0.8317976279585437 1101 20-21 0.9710032989547344 1101 22-23 0.8687918305158746 1101 24-25 0.8849571202845894 1101 26-27 0.6772365486588896 1101 28-29 0.9863109945123867 1101 30-31 1.0329235859288914 1101 32-33 0.9490879748277692 1101 34-35 1.0739660758319687 1101 36-37 0.9084421578874355 1101 38-39 1.3251060228191704 1101 40-41 1.0941207717431993 1101 42-43 1.1157829651532154 1101 44-45 0.8755644851257998 1101 46-47 1.0620358355499562 1101 48-49 1.378870016065413 1101 50-51 1.2539637191057693 1101 52-53 1.417611810040846 1101 54-55 1.4816655850197833 1101 56-57 1.4715432620109326 1101 58-59 1.2816942179240627 1101 60-61 1.3789365659155237 1101 62-63 1.5820742309400586 1101 64-65 1.6401098131157 1101 66-67 1.6872977011880366 1101 68-69 1.3850973596672915 1101 70-71 1.3833581323171629 1101 72-73 1.8240550451522992 1101 74-75 1.7898131587523451 1101 76-77 1.676809727075394 1101 78-79 1.7128649270966108 1101 80-81 1.604327767532567 1101 82-83 1.289645797939965 1101 84-85 1.347337617187879 1101 86-87 1.3300679082505837 1101 88-89 1.4563420159527318 1101 90-91 0.7795469516082427 1101 92-93 1.0744220659127635 1101 94-95 1.5239429307172436 1101 96-97 1.5185897659383514 1101 98-99 1.3000628480429732 1101 100-101 1.24116837369969 1102 1 0.198051427983021 1102 2 -0.009146753631583238 1102 3 0.38343437827168003 1102 4 0.23351238476785596 1102 5 -0.10436734805183079 1102 6 -0.12112352174587926 1102 7 0.054538572675646435 1102 8 0.03203147718649291 1102 9 0.03762304018403739 1102 10-11 -0.11997526653109958 1102 12-13 0.08183162046300652 1102 14-15 0.10039578395747384 1102 16-17 0.2188137516024895 1102 18-19 0.2472905857050236 1102 20-21 0.14637922601468745 1102 22-23 0.04056157944422978 1102 24-25 -0.13467273614869413 1102 26-27 0.07578727329656232 1102 28-29 0.20234147692376325 1102 30-31 0.3359446106278128 1102 32-33 0.5271651334315663 1102 34-35 0.4845940951556429 1102 36-37 0.5589150435103463 1102 38-39 0.523786025540808 1102 40-41 0.46637361245898745 1102 42-43 0.7089992678000172 1102 44-45 0.574992939912498 1102 46-47 0.5593482122254656 1102 48-49 0.6203138481399932 1102 50-51 0.7301153146694901 1102 52-53 0.5581506955276865 1102 54-55 0.5827746548979889 1102 56-57 0.5924863116128947 1102 58-59 0.7622004436844207 1102 60-61 0.6820936728080866 1102 62-63 0.8213407479163166 1102 64-65 0.5090966811840048 1102 66-67 0.6543658103259489 1102 68-69 0.5473413550405013 1102 70-71 0.7095811710325464 1102 72-73 0.6489172619312313 1102 74-75 0.5149951686863474 1102 76-77 1.0707336571291464 1102 78-79 0.7477700097665476 1102 80-81 1.1207256718667864 1102 82-83 1.101410103580573 1102 84-85 1.0280207497999818 1102 86-87 0.8710286485377168 1102 88-89 0.7617444790404342 1102 90-91 0.6362183811516857 1102 92-93 0.9855263729141939 1102 94-95 0.7710341738280846 1102 96-97 0.6598090623934105 1102 98-99 0.8787523765181682 1102 100-101 1.019694603542515 1103 1 0.21382018100940314 1103 2 0.2627116012811541 1103 3 0.16380870194165453 1103 4 0.1135730176232812 1103 5 0.1263322456238285 1103 6 0.3953084031367524 1103 7 0.2635745523804829 1103 8 0.30104766700101493 1103 9 0.38337768765030233 1103 10-11 0.36176119107852855 1103 12-13 0.453797346057236 1103 14-15 0.5206151569969037 1103 16-17 0.457871415974374 1103 18-19 0.4334418731005556 1103 20-21 0.44012225218365586 1103 22-23 0.4961395916129021 1103 24-25 0.49265626025123055 1103 26-27 0.7603911596941728 1103 28-29 0.7951539571864998 1103 30-31 0.5805287071351088 1103 32-33 0.9342052816452195 1103 34-35 0.5294371444829977 1103 36-37 0.5570307933156116 1103 38-39 0.2459591239218284 1103 40-41 0.41746731969492856 1103 42-43 0.6140499004449111 1103 44-45 0.45901155083061695 1103 46-47 0.5499585408639618 1103 48-49 0.2898121533674711 1103 50-51 0.4562990238002058 1103 52-53 0.17542684870985426 1103 54-55 0.18314518901449617 1103 56-57 0.28706935546644985 1103 58-59 0.3508759947486553 1103 60-61 0.18548321785114297 1103 62-63 0.13545262395581403 1103 64-65 0.45140072969029177 1103 66-67 0.08024846048700596 1103 68-69 0.044236026507270765 1103 70-71 0.025187815382949452 1103 72-73 0.07228406965349876 1103 74-75 0.1854934949668987 1103 76-77 0.2399582723842073 1103 78-79 0.1052091137476836 1103 80-81 0.0018494849996955054 1103 82-83 -0.005950051370053444 1103 84-85 0.06614786826562735 1103 86-87 0.39478704345129145 1103 88-89 0.34708311774861755 1103 90-91 0.5153737317906497 1103 92-93 -0.26635261401184707 1103 94-95 0.46197503574096643 1103 96-97 0.4201408588522817 1103 98-99 0.3525097187780055 1103 100-101 0.13669706675226578 1104 1 0.33867767131874515 1104 2 0.4448199846512253 1104 3 0.3645953554340906 1104 4 0.1707289042235729 1104 5 0.3554878282034437 1104 6 0.3698846743231954 1104 7 0.4118036319578948 1104 8 0.5454992897113584 1104 9 0.0038838820715270117 1104 10-11 0.5341884161298367 1104 12-13 0.4673452673158778 1104 14-15 0.5687454298554613 1104 16-17 0.7341217009937537 1104 18-19 0.5874473454726754 1104 20-21 0.6693063367014034 1104 22-23 0.4609681999582733 1104 24-25 0.4606960869594374 1104 26-27 0.44372512640391193 1104 28-29 0.4661275833930887 1104 30-31 0.5465258949438763 1104 32-33 0.5160248453755543 1104 34-35 0.5523907053251378 1104 36-37 0.6160488065405048 1104 38-39 0.40155120418328494 1104 40-41 0.7702548092442214 1104 42-43 0.5047639690015728 1104 44-45 0.5979299972849788 1104 46-47 1.069026337474135 1104 48-49 1.4924989360686922 1104 50-51 1.429944231674341 1104 52-53 1.2746800979308048 1104 54-55 1.4460375656961162 1104 56-57 1.4931288675132706 1104 58-59 1.7612754019083425 1104 60-61 1.729290696759712 1104 62-63 1.7137474480038506 1104 64-65 1.4983244205012625 1104 66-67 1.6396845021378397 1104 68-69 1.6306349016307777 1104 70-71 1.6493042553008692 1104 72-73 1.3743191080361115 1104 74-75 1.737870556607085 1104 76-77 1.4457137637576167 1104 78-79 1.4046789776984312 1104 80-81 1.403261281001818 1104 82-83 1.4526176312323678 1104 84-85 1.6994419322619798 1104 86-87 1.7050781432460766 1104 88-89 1.494457139182071 1104 90-91 1.4392640563327177 1104 92-93 1.4779469983617943 1104 94-95 1.1798628521124783 1104 96-97 1.1724115132567832 1104 98-99 1.2609139902684667 1104 100-101 1.082239363628453 1105 1 0.16670706860406526 1105 2 0.33503729951161887 1105 3 0.20528387080085508 1105 4 0.2970387713028302 1105 5 0.3530966614547211 1105 6 0.20028765624878275 1105 7 0.6027629117947342 1105 8 0.3481049581831215 1105 9 0.1790720106318986 1105 10-11 0.32440748920107865 1105 12-13 0.4364183385585747 1105 14-15 0.43186871559988305 1105 16-17 0.427393028091295 1105 18-19 0.45489582989492305 1105 20-21 0.4157283776325542 1105 22-23 0.13708818026631775 1105 24-25 0.36824936074803105 1105 26-27 0.24867800635328052 1105 28-29 -0.19868710118389288 1105 30-31 0.28083386315542924 1105 32-33 0.4161936336166292 1105 34-35 0.30617085864111715 1105 36-37 0.49054771644978246 1105 38-39 0.5151755800184432 1105 40-41 0.6230661883848043 1105 42-43 0.4422507824202384 1105 44-45 0.7118444779095014 1105 46-47 0.8321162172125085 1105 48-49 0.5300015733849577 1105 50-51 0.46716555525546966 1105 52-53 0.3171848451000798 1105 54-55 0.3345230428745367 1105 56-57 0.2543022974309892 1105 58-59 0.383233697147098 1105 60-61 0.597099614405117 1105 62-63 0.4069484466676059 1105 64-65 0.2903192865256017 1105 66-67 0.43038708319136276 1105 68-69 0.4563082963842717 1105 70-71 0.40445581307567124 1105 72-73 0.2097066177062743 1105 74-75 0.30694186963895476 1105 76-77 0.42286581383145716 1105 78-79 0.19127613642971397 1105 80-81 0.3579609785895599 1105 82-83 0.5543740838661613 1105 84-85 0.48945881108609157 1105 86-87 0.5234549629943004 1105 88-89 0.5094019313615519 1105 90-91 0.5388930738444948 1105 92-93 0.3906166657083041 1105 94-95 0.4246585480123315 1105 96-97 0.30607949272087964 1105 98-99 0.29582326658888647 1105 100-101 0.2730404044892758 1106 1 0.2622469217265184 1106 2 0.4386502094579754 1106 3 0.6514721726810961 1106 4 0.6931741208748718 1106 5 0.7205683189487786 1106 6 0.6547226290781722 1106 7 0.5792208364114799 1106 8 0.556615522602641 1106 9 0.5098763648741595 1106 10-11 0.5449234703813062 1106 12-13 0.5716463807120888 1106 14-15 0.6658484721928843 1106 16-17 0.901264102241889 1106 18-19 0.8478366795624552 1106 20-21 0.8150495464028396 1106 22-23 0.5813320039438139 1106 24-25 0.6403287371260902 1106 26-27 0.5123284169506803 1106 28-29 0.4873317533101158 1106 30-31 0.517157410439232 1106 32-33 0.8439093925707795 1106 34-35 0.800493676745603 1106 36-37 0.7087372683517259 1106 38-39 0.8866143202743544 1106 40-41 1.002959936540158 1106 42-43 1.0832601749444137 1106 44-45 1.1358882981147076 1106 46-47 1.064602892328402 1106 48-49 1.137364548436615 1106 50-51 1.349116630794974 1106 52-53 1.4091829700675333 1106 54-55 1.380660996658463 1106 56-57 1.2758304489613934 1106 58-59 1.2685355253038395 1106 60-61 1.3254629540464222 1106 62-63 1.4850313994966484 1106 64-65 1.4950711051335617 1106 66-67 1.7343940972020206 1106 68-69 1.8264188885820616 1106 70-71 1.8091156161972535 1106 72-73 1.4525493753930334 1106 74-75 1.6014174681736435 1106 76-77 1.6795890208315853 1106 78-79 1.6584109825207847 1106 80-81 1.8231339850653683 1106 82-83 1.9074878532024364 1106 84-85 1.9857868723371475 1106 86-87 2.0721797672443785 1106 88-89 1.9763023983835488 1106 90-91 1.9915919054214335 1106 92-93 1.7690433745053298 1106 94-95 1.8580948923933143 1106 96-97 1.9562123075266946 1106 98-99 2.0464699792054226 1106 100-101 2.129895558224579 1107 1 -0.036648991863515334 1107 2 0.22642231422637593 1107 3 0.30428399875523127 1107 4 0.2341948939196854 1107 5 0.21996927373469077 1107 6 0.3380396806697945 1107 7 0.31031404784678784 1107 8 0.15355947040276163 1107 9 0.11491203110460191 1107 10-11 0.004686064154924452 1107 12-13 0.07402412940800929 1107 14-15 0.18053890405840178 1107 16-17 -0.05152191880763013 1107 18-19 -0.04779121198271952 1107 20-21 0.09883108843738597 1107 22-23 0.19532570014494155 1107 24-25 0.20388282630089094 1107 26-27 -0.1804535490235466 1107 28-29 -0.01714726167523395 1107 30-31 0.18774705525149926 1107 32-33 0.29755250908046804 1107 34-35 0.31278792775476205 1107 36-37 0.3959711540354718 1107 38-39 0.19983202252122823 1107 40-41 0.33699676399670153 1107 42-43 0.4682803581600865 1107 44-45 0.6586520162501088 1107 46-47 0.8561259417450131 1107 48-49 0.891756010659897 1107 50-51 0.824012028284514 1107 52-53 1.1934529643535612 1107 54-55 0.9520948344185882 1107 56-57 0.9195736521022368 1107 58-59 1.0819559813154953 1107 60-61 0.9607642277087152 1107 62-63 0.9758153192798886 1107 64-65 0.7288646280723867 1107 66-67 0.7414167503271969 1107 68-69 1.1245682996745288 1107 70-71 1.2524551550245988 1107 72-73 1.1624640344902275 1107 74-75 0.7767994746986275 1107 76-77 0.9074381720305951 1107 78-79 0.4644770065194592 1107 80-81 0.8523858236733659 1107 82-83 0.78238878034011 1107 84-85 0.5042466810113275 1107 86-87 0.8208849079739196 1107 88-89 0.7192014085765344 1107 90-91 0.9788276343212168 1107 92-93 0.9351447882520354 1107 94-95 0.9340814737805125 1107 96-97 1.1041949075672441 1107 98-99 1.094642430498439 1107 100-101 1.2070433657190982 1108 1 -0.19694569573425014 1108 2 0.03629851349158031 1108 3 -0.3948506149551392 1108 4 -0.015443802951345731 1108 5 0.02126125889949293 1108 6 0.03562460867034645 1108 7 -0.030599396112044985 1108 8 0.04374073139421597 1108 9 -0.02793764938154908 1108 10-11 0.19705603855899056 1108 12-13 0.19563963322999456 1108 14-15 0.08393602408069256 1108 16-17 0.07549136670019152 1108 18-19 -0.16161475675818338 1108 20-21 -0.055127575002224205 1108 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0.43246613712469184 2316 56-57 0.20168818954716272 2316 58-59 0.2953795181310923 2316 60-61 0.48339481298246767 2316 62-63 0.5574641550256416 2316 64-65 0.26390553430272234 2316 66-67 0.5315489089175003 2316 68-69 0.4752353858922831 2316 70-71 0.3647521971894818 2316 72-73 0.518568502709229 2316 74-75 0.5128221304569749 2316 76-77 0.3587282916026524 2316 78-79 0.5015191714030394 2316 80-81 0.36407241901635246 2316 82-83 0.5458500767529451 2316 84-85 0.5568269201554408 2316 86-87 0.5108408551104873 2316 88-89 0.5619389793757783 2316 90-91 0.3056684146862274 2316 92-93 0.7400535358920592 2316 94-95 0.6190153944853627 2316 96-97 0.6766366948548459 2316 98-99 0.6963982517696792 2316 100-101 0.4488495331199793 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3704.0 3 415.0 4 283.0 5 259.0 6 239.0 7 274.0 8 357.0 9 367.0 10 413.0 11 515.0 12 659.0 13 952.0 14 1070.0 15 1504.0 16 1940.0 17 3215.0 18 3082.0 19 1483.0 20 1113.0 21 995.0 22 967.0 23 1048.0 24 1117.0 25 1209.0 26 1329.0 27 1649.0 28 1965.0 29 2287.0 30 2838.0 31 3723.0 32 4584.0 33 6219.0 34 8872.0 35 14697.0 36 22708.0 37 47976.0 38 85197.0 39 29700.0 40 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.51999923264335 21.325992288065684 15.06014157730159 21.093866901989372 2 16.460047961630693 23.013908872901677 39.55299760191846 20.97304556354916 3 17.90505933312104 28.638733612894068 32.41318718419932 21.043019869785574 4 12.737736681566176 21.150651293955146 38.97557887467148 27.136033149807204 5 12.621709340064912 38.00630701828421 35.21111631154522 14.160867330105653 6 25.82563001035633 38.99850408499866 20.08285067699743 15.093015227647577 7 23.343958695343638 35.76796050587463 24.106529802797887 16.781550995983842 8 23.79253735923164 34.27268403449016 22.41860750560005 19.516171100678143 9 25.86536875735923 17.248693077433483 22.77976580868111 34.10617235652617 10-11 22.631107633955196 28.211367615278267 29.20293231626523 19.95459243450131 12-13 24.651631091421667 27.774197878909924 28.498168498168496 19.076002531499913 14-15 22.62804245287543 28.780947229303454 28.03804109097675 20.552969226844368 16-17 19.469894032519203 31.09773409144971 29.208006261365995 20.224365614665103 18-19 19.08707086556777 28.97905472932664 32.633495168910684 19.3003792361949 20-21 21.438011044213294 27.56660957229088 32.44904183106075 18.546337552435077 22-23 21.19845101299837 27.969227405331694 31.666388554835024 19.165933026834914 24-25 19.975720986962976 28.669180228521896 32.43856412459391 18.91653465992122 26-27 19.531888288385048 28.655348724813678 32.82612281219914 18.98664017460214 28-29 19.320947520440043 28.36865150373128 33.06923819003727 19.241162785791413 30-31 19.986805869853715 28.181166146316766 32.7089773782036 19.123050605625917 32-33 19.6186425238708 28.53603296941431 32.38968804475187 19.455636461963017 34-35 19.95642300374394 28.73339010618057 32.31334008469895 18.996846805376542 36-37 19.796002132809594 28.690508613712122 32.1730107522757 19.340478501202583 38-39 19.223843157711034 29.00980168417904 32.75576719130257 19.010587966807364 40-41 19.644781809882897 28.431647060177283 32.86103876676652 19.062532363173297 42-43 19.731588415113592 28.67282399825099 32.02758525462279 19.568002332012625 44-45 19.900071925197793 28.926396547590507 31.80091105250539 19.372620474706306 46-47 19.764317320030152 29.16477265098901 31.3449525201437 19.72595750883714 48-49 19.775683509265956 29.31728679479089 31.337746515076237 19.56928318086692 50-51 20.001764551314878 29.368271449367928 30.82249189169491 19.80747210762229 52-53 20.225104804716114 29.637584716346467 30.41446285904964 19.722847619887773 54-55 20.294774144556687 29.61452316849606 30.218952067561037 19.871750619386212 56-57 20.096320562196002 30.045225783683428 30.109286054040783 19.749167600079787 58-59 19.756784694427452 29.865504736005338 30.60769306005972 19.77001750950749 60-61 20.18759624807504 29.97435941692125 30.280243710194288 19.55780062480942 62-63 20.011775242456846 29.836623305391104 30.5268366213876 19.62476483076445 64-65 20.118575862082196 29.773819707914388 30.156285364646667 19.951319065356742 66-67 19.934022516734114 29.898732234987246 30.104720075183643 20.062525173094997 68-69 19.932792708469194 29.95634594876595 30.123980574330805 19.986880768434055 70-71 19.858732737144315 29.798071815013916 30.403338556220188 19.93985689162158 72-73 19.94545475468065 29.437938481248633 30.277405917123446 20.33920084694727 74-75 19.964371252317882 29.96803384155754 29.77512469102896 20.29247021509562 76-77 19.885535421109967 30.04982891688276 29.832139075997727 20.232496586009542 78-79 19.801720037438816 30.143234161385852 29.916529851318796 20.138515949856536 80-81 19.802982876081753 30.153094887374948 29.795387589762473 20.24853464678083 82-83 19.900796200640258 30.23375806318584 29.79106206758978 20.07438366858412 84-85 19.7452534710701 30.012792947959362 29.70955980367524 20.532393777295297 86-87 19.73584015375438 30.213234462003523 29.762866092133706 20.288059292108386 88-89 20.183503833864588 30.117413301726486 29.605144551462775 20.09393831294615 90-91 19.897462061346303 30.059190437464515 29.89980205919811 20.14354544199107 92-93 19.875965839772263 30.34271486338211 29.78181804230896 19.999501254536668 94-95 19.77871710387523 29.821216921678364 29.960070117027925 20.43999585741848 96-97 20.01952207355871 29.951233169882315 29.654566688592244 20.37467806796673 98-99 19.74705876712723 30.136634289933166 29.620396383266375 20.495910559673227 100-101 19.654991156078903 30.187890158883633 29.811687788482566 20.345430896554902 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1482.0 1 839.0 2 202.0 3 186.5 4 134.0 5 120.0 6 192.5 7 350.0 8 539.5 9 593.5 10 489.5 11 395.0 12 386.5 13 428.5 14 484.5 15 525.5 16 530.0 17 556.5 18 580.0 19 603.5 20 673.5 21 747.0 22 835.0 23 972.5 24 1135.5 25 1358.0 26 1837.5 27 2558.0 28 3174.5 29 3737.0 30 4651.0 31 5664.5 32 6912.0 33 8454.0 34 9873.5 35 10999.5 36 11772.5 37 12238.0 38 11970.0 39 11398.0 40 10738.0 41 10357.5 42 10032.5 43 9564.5 44 9397.5 45 9365.5 46 8849.5 47 8060.0 48 7906.5 49 7514.5 50 6902.0 51 6255.5 52 5544.0 53 4895.0 54 4239.0 55 3658.0 56 3119.5 57 2745.0 58 2439.0 59 2099.5 60 1735.5 61 1354.0 62 1052.5 63 828.5 64 600.0 65 415.0 66 299.0 67 235.5 68 188.0 69 158.0 70 130.0 71 100.5 72 76.5 73 60.5 74 54.0 75 40.0 76 25.0 77 18.0 78 15.5 79 17.5 80 14.0 81 7.5 82 9.0 83 8.5 84 3.5 85 2.0 86 1.5 87 1.5 88 1.0 89 1.0 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13142870280253507 2 0.135260443409023 3 0.1268306140747496 4 0.13142870280253507 5 0.12108300316501774 6 0.1026906482538758 7 0.10767191104231008 8 0.10192430013257824 9 0.09617668922284638 10-11 0.08755527285824859 12-13 0.10077477795063185 14-15 0.11974189395274697 16-17 0.12721378813539838 18-19 0.12357363455923488 20-21 0.11437745710366391 22-23 0.11169523867912239 24-25 0.09924208170803668 26-27 0.10384017043582217 28-29 0.10594762776939051 30-31 0.09809255952609032 32-33 0.09636827625317076 34-35 0.11112047758814918 36-37 0.11073730352750041 38-39 0.0986673206170635 40-41 0.10154112607192944 42-43 0.09924208170803668 44-45 0.1113120646184736 46-47 0.110545716497176 48-49 0.1224241123772885 50-51 0.110545716497176 52-53 0.09770938546544153 54-55 0.09042907831311452 56-57 0.10958778134555403 58-59 0.10058319092030746 60-61 0.09905049467771229 62-63 0.10020001685965867 64-65 0.1153353922552859 66-67 0.10843825916360765 68-69 0.11188682570944677 70-71 0.10211588716290262 72-73 0.10613921479971493 74-75 0.08947114316149254 76-77 0.10958778134555403 78-79 0.10958778134555403 80-81 0.11112047758814918 82-83 0.11591015334625908 84-85 0.11016254243652723 86-87 0.11591015334625908 88-89 0.10537286667841733 90-91 0.11265317383074434 92-93 0.12414839565020808 94-95 0.10384017043582217 96-97 0.09483558001057561 98-99 0.09406923188927803 100-101 0.13296139904513024 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 260978.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.229435939849324 #Duplication Level Percentage of deduplicated Percentage of total 1 71.24206613179004 40.05901193779998 2 16.95125791680871 19.063193422661186 3 5.710612082378319 9.633134887902637 4 2.3174798334761255 5.212423353533539 5 1.1182321814466873 3.143878240626724 6 0.5979691872770001 2.0174082065997507 7 0.39956507741714353 1.5727123247099746 8 0.28563581884617684 1.2848912782350004 9 0.19264896227144285 0.9749288234628493 >10 1.1095548950836065 11.426541623558483 >50 0.060532627174462006 2.2077736330824798 >100 0.010318061450192387 0.7940713505112704 >500 0.0020636122900384774 0.7855620618063273 >1k 0.0020636122900384774 1.824468855509821 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1924 0.7372268926882726 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1775 0.6801339576516029 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1018 0.39007119374046856 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 859 0.3291465180973109 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 605 0.23182030669251816 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 567 0.21725969238786413 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.9158703032439516E-4 0.0 22-23 0.0 0.0 0.0 7.663481212975806E-4 0.0 24-25 0.0 0.0 0.0 0.0030653924851903225 0.0 26-27 0.0 0.0 0.0 0.0034485665458391128 0.0 28-29 0.0 0.0 0.0 0.0068971330916782255 0.0 30-31 0.0 0.0 0.0 0.01111204775881492 0.0 32-33 0.0 0.0 0.0 0.01628489757757359 0.0 34-35 0.0 0.0 0.0 0.024906313942171368 0.0 36-37 0.0 0.0 0.0 0.03007916376093004 0.0 38-39 0.0 0.0 0.0 0.03237820812482278 0.0 40-41 0.0 0.0 0.0 0.03582677467066189 0.0 42-43 0.0 0.0 0.0 0.04214914667136693 0.0 44-45 0.0 0.0 0.0 0.05038738897531593 0.0 46-47 0.0 0.0 0.0 0.05594341285472339 0.0 48-49 0.0 0.0 0.0 0.07069561418970181 0.0 50-51 0.0 0.0 0.0 0.08353194522143628 0.0 52-53 0.0 0.0 0.0 0.10403175746614657 0.0 54-55 0.0 0.0 0.0 0.13890059698518648 0.0 56-57 0.0 0.0 0.0 0.17204515323130687 0.0 58-59 0.0 0.0 0.0 0.20250749105288568 0.0 60-61 0.0 0.0 0.0 0.23507728620803287 0.0 62-63 0.0 0.0 0.0 0.28699737142594395 0.0 64-65 0.0 0.0 0.0 0.3364268252496379 0.0 66-67 0.0 0.0 0.0 0.41555226877361306 0.0 68-69 0.0 0.0 0.0 0.5149859375119742 0.0 70-71 0.0 0.0 0.0 0.6130784970380645 0.0 72-73 0.0 0.0 0.0 0.7176850155951843 0.0 74-75 0.0 0.0 0.0 0.8339783430020922 0.0 76-77 0.0 0.0 0.0 0.9709630696840347 0.0 78-79 0.0 0.0 0.0 1.1217420625493337 0.0 80-81 0.0 0.0 0.0 1.2669650315352252 0.0 82-83 0.0 0.0 0.0 1.4317298776142051 0.0 84-85 0.0 0.0 0.0 1.614503904543678 0.0 86-87 0.0 0.0 0.0 1.8413429484477617 0.0 88-89 0.0 0.0 0.0 2.0879154564752582 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGCG 25 0.0050169337 57.0 5 GGTATCA 1110 0.0 47.92793 1 CGGAGAT 40 5.3804705E-4 47.500004 1 GTATCAA 1890 0.0 37.698414 1 TGGTATC 595 0.0 34.32773 2 GTGGTAT 645 0.0 32.403103 1 TATCAAC 2390 0.0 29.414225 2 GCGTCAG 65 0.0058358964 29.230766 1 ATCAACG 2430 0.0 28.930042 3 TCAACGC 2445 0.0 28.752556 4 CAACGCA 2510 0.0 28.19721 5 GGATAGA 120 5.360769E-6 27.708334 1 AACGCAG 2580 0.0 27.432173 6 CGCGATA 35 0.008387439 27.142859 44-45 GTACTGG 70 0.008379881 27.142859 1 CGCAGAG 2730 0.0 25.924906 8 ACGCAGA 2730 0.0 25.750914 7 GCAGAGT 2870 0.0 24.32927 9 CTTACAC 165 2.4179262E-6 23.030302 3 GAGTACT 1395 0.0 22.302866 12-13 >>END_MODULE