FastQCFastQC Report
Fri 10 Feb 2017
SRR2031708_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031708_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260978
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24420.935711056104346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24420.935711056104346No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT16240.6222746744936355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11250.43107081822988913No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8550.32761382185471577No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7610.29159546015372945No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7540.2889132417291879No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT6940.2659227980902605TruSeq Adapter, Index 10 (95% over 21bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG5210.19963368559801978TruSeq Adapter, Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT4810.18430672317206814RNA PCR Primer, Index 10 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2730.10460651855711976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11500.065.284311
GTATCAA21100.041.661881
CATAACC550.002573873634.551243
TCATAAC550.002573873634.551242
TATCAAC25800.033.881642
ATCAACG25950.033.8688663
TCAACGC26350.033.354734
CAACGCA26850.032.9042325
TGGTATC4300.032.040252
CGCAGTG300.00394575731.67196826-27
AACGCAG27800.031.4380876
GTGGTAT4450.030.9661731
CGCAGAG30000.028.815978
ACGCAGA30550.028.7636247
GCAGAGT32100.026.7828439
GAGTACT19150.025.7957212-13
GTACTTT19700.024.95497914-15
CAGAGTA31950.024.23258810-11
TATAGGT1201.7039475E-423.7539773
CATGGGG7050.023.5855084
Produced by FastQC (version 0.11.5)