FastQCFastQC Report
Fri 10 Feb 2017
SRR2031707_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031707_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1761025
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51610.29306795758152215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43330.24604988571996422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22630.12850470606606948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26300.061.591911
GTATCAA47750.041.683651
ATCAACG58100.033.5203133
TCAACGC58000.033.4962124
TATCAAC60000.032.5398562
CAACGCA59900.032.5111775
AACGCAG61400.031.7124216
ACGCAGA68150.028.22467
CGCAGAG69900.027.1821128
TAGTACG700.00839212227.1432789
GTGGTAT12250.026.3692571
TGGTATC12800.023.38062
GCAGAGT79050.023.3748119
GAGTACT51900.023.20419712-13
CAGAGTA74050.022.26053810-11
GTACTTT58150.020.7104914-15
GTACATG35200.020.3778971
AGAGTAC70750.019.27022210-11
TACATGG37450.018.2656762
AGTACTT57100.017.72145812-13
Produced by FastQC (version 0.11.5)