##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031707_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1761025 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07918740506239 33.0 31.0 34.0 30.0 34.0 2 32.392391930835736 34.0 31.0 34.0 31.0 34.0 3 32.506944535142885 34.0 31.0 34.0 31.0 34.0 4 35.94563620618674 37.0 37.0 37.0 35.0 37.0 5 35.94888658593717 37.0 37.0 37.0 35.0 37.0 6 35.97897247341747 37.0 37.0 37.0 35.0 37.0 7 35.95811643786999 37.0 37.0 37.0 35.0 37.0 8 35.95007453045812 37.0 37.0 37.0 35.0 37.0 9 37.74508084780171 39.0 39.0 39.0 35.0 39.0 10-11 37.67756306696384 39.0 38.0 39.0 35.0 39.0 12-13 37.62748882043128 39.0 38.0 39.0 35.0 39.0 14-15 39.03706477761531 41.0 39.0 41.0 36.0 41.0 16-17 38.9680223165486 41.0 39.0 41.0 36.0 41.0 18-19 38.976632926846584 41.0 39.0 41.0 36.0 41.0 20-21 38.98455501767437 41.0 39.0 41.0 36.0 41.0 22-23 38.88405218551696 41.0 39.0 41.0 35.5 41.0 24-25 38.813998097698786 41.0 39.0 41.0 35.0 41.0 26-27 38.69916923382689 40.5 39.0 41.0 35.0 41.0 28-29 38.58499992901861 40.0 38.0 41.0 35.0 41.0 30-31 38.41418804389489 40.0 38.0 41.0 34.5 41.0 32-33 38.208166266804845 40.0 38.0 41.0 34.0 41.0 34-35 38.034719552533325 40.0 38.0 41.0 34.0 41.0 36-37 37.86802657543193 40.0 38.0 41.0 33.0 41.0 38-39 37.673030763333855 40.0 38.0 41.0 33.0 41.0 40-41 37.57149728141282 40.0 38.0 41.0 33.0 41.0 42-43 37.5341798100538 40.0 38.0 41.0 33.0 41.0 44-45 37.38633097201914 40.0 37.5 41.0 32.5 41.0 46-47 37.28283953947275 40.0 37.5 41.0 32.5 41.0 48-49 37.30369671072245 40.0 38.0 41.0 32.0 41.0 50-51 36.74765718828523 39.5 37.0 40.5 31.5 41.0 52-53 36.76158032963757 39.5 37.0 40.5 31.0 41.0 54-55 36.98649508098977 40.0 37.0 41.0 32.0 41.0 56-57 36.86033560000568 40.0 37.0 41.0 31.0 41.0 58-59 36.663214605130534 40.0 36.0 41.0 31.0 41.0 60-61 36.42151190357888 39.5 36.0 41.0 31.0 41.0 62-63 36.136476483865934 39.0 35.0 41.0 30.0 41.0 64-65 35.857146548175066 39.0 35.0 41.0 30.0 41.0 66-67 35.56190457262106 38.5 35.0 41.0 29.5 41.0 68-69 35.220390965488846 37.5 35.0 40.0 29.0 41.0 70-71 34.84968668815037 37.0 35.0 39.5 29.0 41.0 72-73 34.4728198066467 36.5 35.0 39.0 29.0 41.0 74-75 34.064065246092476 36.0 34.0 39.0 28.0 41.0 76-77 33.68086710864411 35.5 34.0 37.5 28.0 39.5 78-79 33.29570136710154 35.0 34.0 37.0 27.0 39.0 80-81 32.94701722008489 35.0 34.0 37.0 26.5 39.0 82-83 32.62763816527307 35.0 34.0 36.0 26.0 38.0 84-85 32.313587541346664 35.0 34.0 36.0 26.0 37.0 86-87 32.045991964906804 35.0 34.0 35.5 25.5 37.0 88-89 31.78833946139322 35.0 33.0 35.0 25.0 36.5 90-91 31.556831674734884 35.0 33.0 35.0 24.5 36.0 92-93 31.35291094674976 35.0 33.0 35.0 23.5 36.0 94-95 31.120062179696482 35.0 33.0 35.0 21.5 36.0 96-97 30.87124572337133 35.0 33.0 35.0 19.5 35.0 98-99 30.626054144603287 35.0 33.0 35.0 12.5 35.0 100-101 30.051298817450064 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.10611046125270462 1101 2 0.03900097408899228 1101 3 0.12967121717397845 1101 4 0.20947366077363228 1101 5 0.07761260589023777 1101 6 0.10422993391669166 1101 7 0.19145403001972028 1101 8 0.18059178799776276 1101 9 0.19770582640862955 1101 10-11 0.0999731211338073 1101 12-13 0.07937382991288189 1101 14-15 0.054268854119477794 1101 16-17 0.016575399984269268 1101 18-19 0.1315522998998162 1101 20-21 0.1562853390408776 1101 22-23 0.09222121110222758 1101 24-25 -0.0724938216666331 1101 26-27 -0.2541507357866877 1101 28-29 -0.18822830089607834 1101 30-31 -0.015544030673872555 1101 32-33 -0.33261089564149415 1101 34-35 -0.21333208055020236 1101 36-37 -0.5050404260427754 1101 38-39 -0.14744313070541182 1101 40-41 -0.377739766909734 1101 42-43 -0.27551116378317175 1101 44-45 -0.3542877435048055 1101 46-47 -0.3248828374846582 1101 48-49 -0.11950275463309623 1101 50-51 -0.14907879947697467 1101 52-53 -0.17608169122016193 1101 54-55 0.034986297161367474 1101 56-57 -0.15445196572546394 1101 58-59 -0.37357744316661723 1101 60-61 -0.4673816557004784 1101 62-63 -0.2963655291135794 1101 64-65 -0.0658640928893206 1101 66-67 -0.3280205046907909 1101 68-69 -0.4796213561800684 1101 70-71 -0.5562009078226566 1101 72-73 -0.1759140732481157 1101 74-75 -0.06975558108425872 1101 76-77 -0.2610101626469401 1101 78-79 -0.4822993893306844 1101 80-81 -0.5773921005031966 1101 82-83 -0.6498883083984666 1101 84-85 -0.6148075019251742 1101 86-87 -0.7243976598715172 1101 88-89 -0.9547816087069911 1101 90-91 -1.2773410595831116 1101 92-93 -1.1106382818078053 1101 94-95 -1.2590745200821907 1101 96-97 -1.3788532696282694 1101 98-99 -1.286651175112187 1101 100-101 -1.4718920557055206 1102 1 0.021934583792344142 1102 2 0.14160457769259693 1102 3 0.12610364960641363 1102 4 0.15579798509795495 1102 5 0.13898197525960398 1102 6 0.13547317715993046 1102 7 0.12389547146116087 1102 8 -0.012822626416657101 1102 9 0.20284096154376385 1102 10-11 0.27850014816083046 1102 12-13 0.2559368929759458 1102 14-15 0.4378814667320938 1102 16-17 0.30345377836265186 1102 18-19 0.288002750350266 1102 20-21 0.2485871408426803 1102 22-23 0.3673518417328623 1102 24-25 0.16112779995498983 1102 26-27 0.09742133628538596 1102 28-29 0.11449241982463576 1102 30-31 0.04792894229910161 1102 32-33 0.2324431584125577 1102 34-35 0.3624697212515997 1102 36-37 0.19073434873200057 1102 38-39 -0.025402590164866012 1102 40-41 0.07603951236954742 1102 42-43 0.23318703441502464 1102 44-45 -0.00985080656786863 1102 46-47 0.056819865218045607 1102 48-49 0.08969544356509829 1102 50-51 0.06227255187437919 1102 52-53 0.14843632679786367 1102 54-55 0.31378359445866266 1102 56-57 0.13112911535561267 1102 58-59 0.13926039467122564 1102 60-61 -0.002944718763544074 1102 62-63 0.10045429070623868 1102 64-65 0.06372599720077687 1102 66-67 -0.09659708126736177 1102 68-69 0.10957684201812867 1102 70-71 0.017055848934091955 1102 72-73 -0.12334200117604865 1102 74-75 -0.3977330585617338 1102 76-77 -0.05068583832261453 1102 78-79 -0.006182272213564488 1102 80-81 -0.005982190593293524 1102 82-83 0.06106214205198057 1102 84-85 0.10847628185860714 1102 86-87 0.16916540319154905 1102 88-89 0.12126343633805448 1102 90-91 0.10480758906553334 1102 92-93 0.12565000648048397 1102 94-95 -0.09057902458669176 1102 96-97 -0.2838262426012399 1102 98-99 -0.12233585979687334 1102 100-101 -0.22468935300281956 1103 1 -0.12391308415802271 1103 2 0.06052284898057536 1103 3 0.13861199363721965 1103 4 0.07808553323659595 1103 5 0.13429476944018148 1103 6 0.10209448653092323 1103 7 0.06371489807647635 1103 8 0.14967116904761468 1103 9 0.0897087407478736 1103 10-11 0.015751752782144024 1103 12-13 0.10719406028445633 1103 14-15 0.18355883087415492 1103 16-17 0.18877598632157344 1103 18-19 0.12961721333699217 1103 20-21 0.015184915767797236 1103 22-23 0.06193506809143656 1103 24-25 0.10580302460197544 1103 26-27 0.0647816272567141 1103 28-29 -0.07544510691904804 1103 30-31 0.1322203415288854 1103 32-33 -0.03147725237049315 1103 34-35 -0.07017383886821449 1103 36-37 0.0565657565197597 1103 38-39 0.14227905296735344 1103 40-41 0.1335944973930765 1103 42-43 0.08025549825755718 1103 44-45 -0.06914263369666429 1103 46-47 0.15182500000623378 1103 48-49 0.23040703962842457 1103 50-51 0.20612364301687336 1103 52-53 0.2121599040336335 1103 54-55 -0.020909601946982548 1103 56-57 0.04099047607447659 1103 58-59 0.17725782727713124 1103 60-61 0.1280009380614473 1103 62-63 0.07827885210974728 1103 64-65 0.17688431983663122 1103 66-67 0.06066821612245121 1103 68-69 -0.03369102276577962 1103 70-71 0.11337677319596651 1103 72-73 -0.0336826087926454 1103 74-75 -0.0895362250144558 1103 76-77 -0.10225066502351154 1103 78-79 -0.15663498937232134 1103 80-81 -0.14045501900578472 1103 82-83 -0.13980478135409413 1103 84-85 -0.2514184549834937 1103 86-87 -0.19545419458337676 1103 88-89 -0.17749437281898395 1103 90-91 -0.0947225778150802 1103 92-93 -0.6829832840629457 1103 94-95 -0.3221370049033361 1103 96-97 -0.17814203207492696 1103 98-99 -0.2531822440501905 1103 100-101 -0.21840308856122803 1104 1 0.0015003871193570717 1104 2 0.1022750346308996 1104 3 0.19488949925897003 1104 4 0.13550360733421485 1104 5 0.15223571288858295 1104 6 0.1926721664208273 1104 7 0.14648254242347036 1104 8 0.23550249466146767 1104 9 0.1406110183978413 1104 10-11 0.19884948486329534 1104 12-13 0.17730475989529282 1104 14-15 0.3828901001285985 1104 16-17 0.4168031150651146 1104 18-19 0.2787125839994502 1104 20-21 0.3017252751867474 1104 22-23 0.4051928608947932 1104 24-25 0.3904417607888533 1104 26-27 0.464954922303491 1104 28-29 0.3958320702773648 1104 30-31 0.49439198715064947 1104 32-33 0.4928108147612704 1104 34-35 0.55762112154639 1104 36-37 0.46562295686002386 1104 38-39 0.3696687932842764 1104 40-41 0.36775608427942075 1104 42-43 0.46065028983721845 1104 44-45 0.5001502462853651 1104 46-47 0.7432020509413562 1104 48-49 0.8317171323416872 1104 50-51 0.6833211936937076 1104 52-53 0.7010356108576588 1104 54-55 0.8222788566191213 1104 56-57 0.7761158494048814 1104 58-59 0.770309036490552 1104 60-61 0.8742483745192544 1104 62-63 0.7750072492238189 1104 64-65 0.765426776312168 1104 66-67 0.7565473701961025 1104 68-69 0.8454772421023549 1104 70-71 0.8790065754028262 1104 72-73 0.6976135684175588 1104 74-75 0.7676411254770059 1104 76-77 0.7121928729850282 1104 78-79 0.631948702728522 1104 80-81 0.6856474156396004 1104 82-83 0.7216323673625737 1104 84-85 0.8270385054846372 1104 86-87 0.618084754633955 1104 88-89 0.7456119307579279 1104 90-91 0.8352131481306024 1104 92-93 0.6950010277481162 1104 94-95 0.9292089307723295 1104 96-97 0.7892666190538442 1104 98-99 0.6582229946988072 1104 100-101 0.5983485497135419 1105 1 -0.11623730079943329 1105 2 0.14719328241942264 1105 3 0.16191948754379837 1105 4 0.220415541881259 1105 5 0.27033986353462325 1105 6 0.26757753566207754 1105 7 0.31799369122789045 1105 8 0.2399803201763504 1105 9 0.16101775712387223 1105 10-11 0.2667868271049727 1105 12-13 0.2628098211527359 1105 14-15 0.2993707239451098 1105 16-17 0.32513333637370323 1105 18-19 0.259826568643696 1105 20-21 0.2652924815424953 1105 22-23 0.09789020882923438 1105 24-25 0.19639606269286247 1105 26-27 0.22692225709568703 1105 28-29 0.057968171819730685 1105 30-31 0.1956348968724555 1105 32-33 0.4124959515371813 1105 34-35 0.18607561443759835 1105 36-37 0.19807995953617308 1105 38-39 0.24192337668596053 1105 40-41 0.3042789230509442 1105 42-43 0.2123048981350948 1105 44-45 0.3946274009213866 1105 46-47 0.4184582937017822 1105 48-49 0.518290287027348 1105 50-51 0.48833762246983525 1105 52-53 0.4492797890446383 1105 54-55 0.5336264428318955 1105 56-57 0.3386687101990731 1105 58-59 0.32056794531579413 1105 60-61 0.45383244514069077 1105 62-63 0.43052918327837375 1105 64-65 0.4677266110743119 1105 66-67 0.3877755399726581 1105 68-69 0.5246848837615303 1105 70-71 0.5455297593085575 1105 72-73 0.3302356538270246 1105 74-75 0.4038642403946753 1105 76-77 0.6357665864778781 1105 78-79 0.6853855608128328 1105 80-81 0.6624825116780428 1105 82-83 0.700907445919384 1105 84-85 0.6899323899003562 1105 86-87 0.7112636229582776 1105 88-89 0.696003153956223 1105 90-91 0.658961057451048 1105 92-93 0.6760539352711525 1105 94-95 0.714352221576597 1105 96-97 0.6383824744030555 1105 98-99 0.665112881762365 1105 100-101 0.7642283879425484 1106 1 -0.07979907140781961 1106 2 0.10267800356264445 1106 3 0.15250799480858745 1106 4 0.15208404424098632 1106 5 0.12265085476071391 1106 6 0.16886726285697762 1106 7 0.08732616950984351 1106 8 0.10658674795470091 1106 9 0.18335393680025192 1106 10-11 0.2650583859399518 1106 12-13 0.15951846610006015 1106 14-15 0.20101443796524165 1106 16-17 0.24583639755396547 1106 18-19 0.15478991591051283 1106 20-21 0.14850405896780927 1106 22-23 0.17991530984792803 1106 24-25 0.3075466291644062 1106 26-27 0.20581864065069766 1106 28-29 0.2264171834536839 1106 30-31 0.2902518149605342 1106 32-33 0.12091509564852743 1106 34-35 0.16380667558674133 1106 36-37 0.18277498441898388 1106 38-39 0.20757387332336208 1106 40-41 0.09464703600663427 1106 42-43 0.002291238800459894 1106 44-45 0.0863280306882146 1106 46-47 0.0888035707098993 1106 48-49 0.13087810298976166 1106 50-51 0.20287605217612992 1106 52-53 0.3084697204812272 1106 54-55 0.2775687483512357 1106 56-57 0.07796435977323313 1106 58-59 0.2853376427098482 1106 60-61 0.17225343050219522 1106 62-63 0.2897739446993981 1106 64-65 0.2971162607292399 1106 66-67 0.368129734262709 1106 68-69 0.3269755545508204 1106 70-71 0.3170389509256495 1106 72-73 0.2868012295622364 1106 74-75 0.3148998120880293 1106 76-77 0.3700884525645307 1106 78-79 0.37111324176751737 1106 80-81 0.4028230799760095 1106 82-83 0.446878275626716 1106 84-85 0.5083169577789519 1106 86-87 0.444084332984346 1106 88-89 0.4219371438415713 1106 90-91 0.46764886848617593 1106 92-93 0.5059742873092894 1106 94-95 0.36847689977460263 1106 96-97 0.39992390827383417 1106 98-99 0.40398600703072773 1106 100-101 0.436717406200561 1107 1 -0.1303092332354545 1107 2 0.05045640522597239 1107 3 0.09440831145801809 1107 4 0.02591879928048968 1107 5 0.0257191168660853 1107 6 0.032929513338530114 1107 7 0.11582937384680037 1107 8 0.1249703260893682 1107 9 0.21445619463634813 1107 10-11 0.10401098204133774 1107 12-13 0.06360946903197373 1107 14-15 0.16126207518033198 1107 16-17 0.16044130298511305 1107 18-19 0.11443326030867951 1107 20-21 0.12238731593570407 1107 22-23 0.1889686068893468 1107 24-25 0.22398006522090697 1107 26-27 0.05232985018339065 1107 28-29 0.07429587791174441 1107 30-31 0.0048764143936494975 1107 32-33 0.13974585046771892 1107 34-35 0.11549970593095793 1107 36-37 0.12993898869697773 1107 38-39 0.2264280073400542 1107 40-41 0.22673375618657587 1107 42-43 0.21608548046449272 1107 44-45 0.27907346570177083 1107 46-47 0.3114377247059039 1107 48-49 0.23958404063228755 1107 50-51 0.18782737787569204 1107 52-53 0.2639351230333631 1107 54-55 0.3650560829681808 1107 56-57 0.4725828251389359 1107 58-59 0.4698250696548101 1107 60-61 0.48392111621129175 1107 62-63 0.34072831127966907 1107 64-65 0.2750932548063787 1107 66-67 0.1532438029523746 1107 68-69 0.31469196567757507 1107 70-71 0.33564284827750157 1107 72-73 0.3150379506733785 1107 74-75 0.2624818681180656 1107 76-77 0.20755250704836214 1107 78-79 0.2582309473093005 1107 80-81 0.3454883718930333 1107 82-83 0.3092976421614182 1107 84-85 0.24708691875946442 1107 86-87 0.32393843886674745 1107 88-89 0.20675500998655494 1107 90-91 0.12220220495522582 1107 92-93 0.1592433529457935 1107 94-95 0.2614921069519376 1107 96-97 0.40972989659770676 1107 98-99 0.4758681235692883 1107 100-101 0.5417512248041412 1108 1 0.022053263433633674 1108 2 -0.028779700116977835 1108 3 0.1444161853820276 1108 4 0.10477680593367467 1108 5 0.11045268695584554 1108 6 0.23044398425781765 1108 7 0.19685750160807913 1108 8 0.14643400399739193 1108 9 0.17502756715337853 1108 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>>Per sequence quality scores pass #Quality Count 2 7307.0 3 934.0 4 805.0 5 865.0 6 978.0 7 1221.0 8 1513.0 9 1831.0 10 2236.0 11 2756.0 12 3580.0 13 4652.0 14 5543.0 15 6522.0 16 7539.0 17 10569.0 18 9903.0 19 6207.0 20 5544.0 21 5582.0 22 5737.0 23 6246.0 24 7081.0 25 8199.0 26 9493.0 27 11257.0 28 13712.0 29 16775.0 30 21265.0 31 27134.0 32 35311.0 33 47520.0 34 69036.0 35 109320.0 36 189048.0 37 377373.0 38 545653.0 39 174536.0 40 242.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.204216703056765 19.578784570596795 14.031113537117903 24.18588518922853 2 16.208283200010918 21.471258537828007 38.918052772476976 23.402405489684096 3 18.487785324194242 25.34017154277624 30.8077066317175 25.364336501312014 4 13.464062247676061 18.571610528973327 37.24119382345114 30.723133399899467 5 14.167106346397887 36.630343814388446 34.798584989333975 14.403964849879694 6 30.483036178685012 36.42851661422761 18.601397806370564 14.487049400716808 7 25.76339924494322 32.67903037565809 23.360542740409297 18.1970276389894 8 24.345542000475202 34.13214327534245 21.877660947157924 19.644653777024423 9 25.520214309708905 16.323324369658103 21.81424331973151 36.34221800090148 10-11 23.740141392199956 26.36497351809005 29.433794656786606 20.46109043292339 12-13 24.81315328138536 24.859424418882263 28.59507979781673 21.732342501915646 14-15 22.408966372407942 26.61919525062213 26.74811229626985 24.223726080700082 16-17 21.67188313370124 28.620791513700368 27.72651971477691 21.980805637821486 18-19 21.513713841888542 27.757438020758112 29.572664200498767 21.15618393685458 20-21 22.321814874974912 27.117120087873754 29.320672675390746 21.24039236176059 22-23 22.145863090796684 27.035311005464713 28.961164289622726 21.857661614115877 24-25 21.87279472305203 27.23167987713117 29.247539255528345 21.647986144288456 26-27 21.77922907330599 27.26588788220973 29.339734747920144 21.615148296564136 28-29 21.745875417645497 27.36033835147453 29.339171487976216 21.55461474290376 30-31 22.111585601879824 27.107143527047207 29.18036756392978 21.600903307143184 32-33 21.875069274278395 27.27769564335129 29.037106003429752 21.810129078940562 34-35 22.13242199669363 27.452263409110035 28.884759082857126 21.530555511339216 36-37 22.072259142114913 27.33318040453363 29.055448628572616 21.539111824778843 38-39 21.877660947157924 27.430393317121805 29.08712957057642 21.604816165143852 40-41 22.14990684646507 27.27922043778609 28.923497570618338 21.647375145130507 42-43 22.097313133018375 27.42216344816159 28.680235289503457 21.800288129316574 44-45 22.170601337822305 27.514642025085024 28.498224537202855 21.81653209988982 46-47 22.11322842071961 27.46038346931344 28.455085347263065 21.971302762703882 48-49 22.044293557752965 27.656082581845403 28.43556800604507 21.864055854356565 50-51 22.161727180500574 27.769071785164968 28.191554830271993 21.877646204062465 52-53 22.225335860875298 27.810758347851543 28.02519205245313 21.93871373882003 54-55 22.22106022378953 27.770016386072466 28.020553352494943 21.988370037643065 56-57 22.129781329125077 27.909500523568543 27.93678748347149 22.023930663834886 58-59 22.12215458309187 27.8888017623374 28.155221430441134 21.833822224129595 60-61 22.237424169865186 27.775379381592728 28.1724457909568 21.81475065758529 62-63 22.092231104401243 27.86887482304405 28.073647780849452 21.96524629170526 64-65 22.148002243466102 27.88743212676436 28.026270044297934 21.938295585471597 66-67 22.039715665351945 27.94305916702955 27.901985953813814 22.115239213804692 68-69 22.01456219605239 27.91090419332575 28.016024442021266 22.058509168600597 70-71 22.100075006942195 27.753705510636973 28.082155766027228 22.064063716393605 72-73 22.034331392242155 27.88904060374681 27.929941085116205 22.14668691889483 74-75 22.08130901038742 27.861144139371934 27.90323149009114 22.1543153601495 76-77 22.00531670555275 27.913016832444182 27.860742337622174 22.220924124380893 78-79 21.903996848161725 27.916651387869116 27.91622500282125 22.26312676114791 80-81 21.93882177437946 27.99166332902306 27.870058451638467 22.19945644495901 82-83 22.02911577830027 27.945313892492045 27.860543773873577 22.16502655533411 84-85 22.04023600285723 27.953382699092945 27.731103185731588 22.275278112318237 86-87 21.969374583013177 27.974837661183976 27.79839200499631 22.25739575080654 88-89 22.0371101483161 27.91131560793797 27.73719391205064 22.314380331695293 90-91 21.980351630821467 27.81411679696505 27.87818818397414 22.32734338823935 92-93 22.038008064901305 27.77063247839921 27.92477762387902 22.266581832820464 94-95 21.991231498390544 27.841229011376313 27.820593898998798 22.34694559123434 96-97 21.97129703307946 27.81649994316244 27.831192451972264 22.381010571785836 98-99 21.864535247396947 27.84443041762809 27.857759366712926 22.433274968262037 100-101 21.88570549619766 27.85606183595537 27.82086315246574 22.437369515381228 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3382.0 1 1955.0 2 513.0 3 493.5 4 410.0 5 388.5 6 510.0 7 867.0 8 1376.5 9 1612.5 10 1404.0 11 1151.0 12 1172.0 13 1260.0 14 1417.0 15 1570.5 16 1648.5 17 1779.0 18 1972.0 19 2198.0 20 2444.0 21 2807.5 22 3460.5 23 4296.0 24 5364.5 25 6953.0 26 9056.0 27 11894.5 28 15069.0 29 18846.0 30 22862.0 31 26511.5 32 30699.0 33 34887.5 34 38946.5 35 42765.0 36 46922.5 37 50840.5 38 53678.5 39 56351.0 40 58816.5 41 60684.5 42 62887.0 43 66240.0 44 68748.5 45 69898.5 46 70459.5 47 69966.0 48 69205.5 49 68349.0 50 65774.5 51 61941.5 52 58547.5 53 55749.0 54 52028.0 55 47764.0 56 42976.0 57 37417.0 58 33082.5 59 28809.5 60 23507.0 61 18809.0 62 15079.0 63 11448.5 64 8357.0 65 6220.0 66 4614.0 67 3583.5 68 2754.5 69 1977.5 70 1466.5 71 1094.5 72 787.0 73 535.5 74 352.5 75 252.0 76 200.0 77 150.5 78 104.5 79 76.5 80 52.5 81 40.0 82 33.5 83 30.0 84 24.0 85 20.0 86 13.5 87 8.5 88 9.5 89 7.0 90 6.0 91 6.0 92 3.0 93 2.5 94 3.0 95 2.5 96 1.5 97 1.5 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13088968072571372 2 0.13520534915745092 3 0.12947005295211594 4 0.13475106826989963 5 0.12316690563734188 6 0.10448460413679532 7 0.11084453656251331 8 0.10124785281299244 9 0.09664825882653569 10-11 0.08642693885663179 12-13 0.10385996791641232 14-15 0.1216337076418563 16-17 0.13239448616572733 18-19 0.13009468917249897 20-21 0.12140656719808066 22-23 0.1142800357746199 24-25 0.09940233670731535 26-27 0.10394514558282819 28-29 0.1083175991255093 30-31 0.09721610993597479 32-33 0.09732968015786261 34-35 0.11339986655498928 36-37 0.11564287843727375 38-39 0.10124785281299244 40-41 0.104058715804716 42-43 0.0991184111525958 44-45 0.1162391221021848 46-47 0.11388253999801252 48-49 0.12699990062605584 50-51 0.11467753155122726 52-53 0.09880609304240427 54-55 0.09108331795403246 56-57 0.11033347056401811 58-59 0.10053803892619356 60-61 0.09923198137448361 62-63 0.09991340270581053 64-65 0.12149174486449651 66-67 0.11192345367044759 68-69 0.11851052653994122 70-71 0.10530798824548203 72-73 0.10658565324172002 74-75 0.0902315412898738 76-77 0.11589841143652135 78-79 0.1164946551014324 80-81 0.11638108487954457 82-83 0.12211638108487954 84-85 0.11234934200252693 86-87 0.12075353842222569 88-89 0.10848795445834103 90-91 0.11638108487954457 92-93 0.1302082593943868 94-95 0.10718189690663109 96-97 0.09227580528385457 98-99 0.09633594071634417 100-101 0.13835692281483794 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1761025.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.98034714055919 #Duplication Level Percentage of deduplicated Percentage of total 1 66.73399819985441 39.359943799048644 2 19.245874287572125 22.702566930091326 3 7.163623949160424 12.675390819177162 4 3.0305448888348394 7.149703582741047 5 1.5119853535575853 4.458871051213375 6 0.7869596443388632 2.7849091805230293 7 0.4695089418164348 1.9384260261750952 8 0.285289511997294 1.3461179562528902 9 0.18845671495631328 1.0003718222183493 >10 0.572216438978876 5.041315704758203 >50 0.008357947311497773 0.32933323388297114 >100 0.0025088359967377306 0.29892707213565717 >500 9.646937494342367E-5 0.030838700946469722 >1k 4.823468747171177E-4 0.5896336861776114 >5k 9.646937494342355E-5 0.29365043465817353 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5161 0.29306795758152215 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4333 0.24604988571996422 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2263 0.12850470606606948 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1357022188782101E-4 2 0.0 0.0 0.0 0.0 1.1357022188782101E-4 3 0.0 0.0 0.0 0.0 1.1357022188782101E-4 4 0.0 0.0 0.0 0.0 1.1357022188782101E-4 5 0.0 0.0 0.0 0.0 1.1357022188782101E-4 6 0.0 0.0 0.0 0.0 1.1357022188782101E-4 7 0.0 0.0 0.0 0.0 1.1357022188782101E-4 8 0.0 0.0 0.0 0.0 1.1357022188782101E-4 9 0.0 0.0 0.0 0.0 1.1357022188782101E-4 10-11 0.0 0.0 0.0 0.0 1.1357022188782101E-4 12-13 0.0 0.0 0.0 0.0 1.9874788830368678E-4 14-15 0.0 0.0 0.0 0.0 3.40710665663463E-4 16-17 0.0 0.0 0.0 0.0 4.5428088755128404E-4 18-19 0.0 0.0 0.0 0.0 5.678511094391051E-4 20-21 0.0 0.0 0.0 8.517766641586576E-5 6.81421331326926E-4 22-23 0.0 0.0 0.0 2.839255547195525E-4 6.81421331326926E-4 24-25 0.0 0.0 0.0 7.098138867988814E-4 6.81421331326926E-4 26-27 0.0 0.0 0.0 9.937394415184338E-4 6.81421331326926E-4 28-29 0.0 0.0 0.0 0.0014764128845416733 7.382064422708366E-4 30-31 0.0 0.0 0.0 0.002981218324555302 7.665989977427919E-4 32-33 0.0 0.0 0.0 0.0054797632060873645 8.517766641586576E-4 34-35 0.0 0.0 0.0 0.008574551752530486 8.517766641586576E-4 36-37 0.0 0.0 0.0 0.011555770077085789 8.517766641586576E-4 38-39 0.0 0.0 0.0 0.01399752984767394 8.517766641586576E-4 40-41 0.0 0.0 0.0 0.01572947573146321 9.653468860464787E-4 42-43 0.0 0.0 0.0 0.020016751607728454 9.653468860464787E-4 44-45 0.0 0.0 0.0 0.025269374370040176 0.0010221319969903892 46-47 0.0 0.0 0.0 0.03126020357462274 0.0010789171079342997 48-49 0.0 0.0 0.0 0.045229340866824716 0.0010789171079342997 50-51 0.0 0.0 0.0 0.05525191294842492 0.0011357022188782102 52-53 0.0 0.0 0.0 0.07123692167913573 0.0013060575517099416 54-55 0.0 0.0 0.0 0.0962507630499283 0.0013060575517099416 56-57 0.0 0.0 0.0 0.1225138768614869 0.0013060575517099416 58-59 0.0 0.0 0.0 0.15011144078022742 0.0013060575517099416 60-61 0.0 0.0 0.0 0.18318876790505528 0.0013060575517099416 62-63 0.0 0.0 0.0 0.22205817634616204 0.001362842662653852 64-65 0.0 0.0 0.0 0.26646413310430006 0.0014196277735977626 66-67 0.0 0.0 0.0 0.32972274669581636 0.0014196277735977626 68-69 0.0 0.0 0.0 0.40910833179540324 0.0014196277735977626 70-71 0.0 0.0 0.0 0.48485966979457984 0.0014764128845416733 72-73 0.0 0.0 0.0 0.5688732414360955 0.0015331979954855838 74-75 5.6785110943910504E-5 0.0 0.0 0.6634204511577064 0.0015331979954855838 76-77 5.6785110943910504E-5 0.0 0.0 0.7663434647435443 0.0015331979954855838 78-79 5.6785110943910504E-5 0.0 0.0 0.8791754801890944 0.0015331979954855838 80-81 5.6785110943910504E-5 0.0 0.0 0.994250507516929 0.0015331979954855838 82-83 5.6785110943910504E-5 0.0 0.0 1.1253673286864183 0.0015899831064294942 84-85 5.6785110943910504E-5 0.0 0.0 1.2870345395437317 0.0015899831064294942 86-87 5.6785110943910504E-5 0.0 0.0 1.4846183330730682 0.0017319458837892704 88-89 5.6785110943910504E-5 0.0 0.0 1.6965403671157424 0.0018739086611490466 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2630 0.0 61.59191 1 GTATCAA 4775 0.0 41.68365 1 ATCAACG 5810 0.0 33.520313 3 TCAACGC 5800 0.0 33.496212 4 TATCAAC 6000 0.0 32.539856 2 CAACGCA 5990 0.0 32.511177 5 AACGCAG 6140 0.0 31.712421 6 ACGCAGA 6815 0.0 28.2246 7 CGCAGAG 6990 0.0 27.182112 8 TAGTACG 70 0.008392122 27.143278 9 GTGGTAT 1225 0.0 26.369257 1 TGGTATC 1280 0.0 23.3806 2 GCAGAGT 7905 0.0 23.374811 9 GAGTACT 5190 0.0 23.204197 12-13 CAGAGTA 7405 0.0 22.260538 10-11 GTACTTT 5815 0.0 20.71049 14-15 GTACATG 3520 0.0 20.377897 1 AGAGTAC 7075 0.0 19.270222 10-11 TACATGG 3745 0.0 18.265676 2 AGTACTT 5710 0.0 17.721458 12-13 >>END_MODULE