FastQCFastQC Report
Fri 10 Feb 2017
SRR2031707_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031707_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1761025
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61330.34826308541900314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55250.31373773796510557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30320.17217245638193665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29350.057.6299971
GTATCAA50300.041.6559751
TCAACGC59600.034.6697774
ATCAACG60200.034.2453233
CAACGCA61750.033.3050165
TATCAAC63250.032.5939672
AACGCAG64350.032.1807826
ACGCAGA71350.028.7573227
CGCAGAG73050.027.9580548
GTGGTAT11700.027.207941
GCAGAGT83500.024.060949
GAGTACT57700.023.04906312-13
CAGAGTA79650.022.48156710-11
TGGTATC14150.021.484812
GTACATG34350.020.1944871
GTACTTT65750.019.97424714-15
AGAGTAC74250.019.6387110-11
TACATGG36350.018.6870022
AGTACTT63000.017.6419412-13
TATGCCG6650.017.49859442-43
Produced by FastQC (version 0.11.5)