##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031707_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1761025 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68916000624636 34.0 31.0 34.0 31.0 34.0 2 32.85814284294658 34.0 31.0 34.0 31.0 34.0 3 32.90971508070584 34.0 31.0 34.0 31.0 34.0 4 36.325878962536024 37.0 37.0 37.0 35.0 37.0 5 36.28870458965659 37.0 37.0 37.0 35.0 37.0 6 36.3199579790179 37.0 37.0 37.0 35.0 37.0 7 36.26323419599381 37.0 37.0 37.0 35.0 37.0 8 36.25394131258784 37.0 37.0 37.0 35.0 37.0 9 38.09921835294786 39.0 39.0 39.0 35.0 39.0 10-11 38.054988713959204 39.0 39.0 39.0 35.0 39.0 12-13 38.00270978549425 39.0 38.5 39.0 35.0 39.0 14-15 39.46860919067021 41.0 39.0 41.0 36.0 41.0 16-17 39.44274414048637 41.0 39.0 41.0 36.0 41.0 18-19 39.453576184324476 41.0 39.0 41.0 36.0 41.0 20-21 39.44814497238824 41.0 39.0 41.0 36.0 41.0 22-23 39.35019349526554 41.0 39.0 41.0 36.0 41.0 24-25 39.281541147911014 41.0 39.0 41.0 36.0 41.0 26-27 39.13896083246973 41.0 39.0 41.0 36.0 41.0 28-29 39.02776082111271 40.0 39.0 41.0 35.0 41.0 30-31 38.84331625047913 40.0 38.0 41.0 35.0 41.0 32-33 38.62469101801507 40.0 38.0 41.0 35.0 41.0 34-35 38.47268522593376 40.0 38.0 41.0 34.5 41.0 36-37 38.35950057494925 40.0 38.0 41.0 34.0 41.0 38-39 38.43232776366037 40.0 38.0 41.0 34.5 41.0 40-41 38.34484604136796 40.0 38.0 41.0 34.0 41.0 42-43 38.23286835791655 40.0 38.0 41.0 34.0 41.0 44-45 38.034733748811064 40.0 38.0 41.0 33.5 41.0 46-47 37.79382007637598 40.0 38.0 41.0 33.0 41.0 48-49 37.52896211013472 40.0 37.5 41.0 33.0 41.0 50-51 37.3657574423986 40.0 37.0 41.0 32.5 41.0 52-53 37.41819764057864 40.0 37.0 41.0 33.0 41.0 54-55 37.34772050368393 40.0 37.0 41.0 33.0 41.0 56-57 37.172472281767725 40.0 37.0 41.0 32.5 41.0 58-59 36.989483113527626 40.0 36.0 41.0 32.0 41.0 60-61 36.735860365412194 39.5 36.0 41.0 31.5 41.0 62-63 36.46404196419699 39.0 35.0 41.0 31.0 41.0 64-65 36.149404750074524 39.0 35.0 41.0 31.0 41.0 66-67 35.814255618176915 38.0 35.0 40.5 30.5 41.0 68-69 35.442110986499344 37.0 35.0 40.0 30.0 41.0 70-71 35.08927272469158 37.0 35.0 39.5 30.0 41.0 72-73 34.698213824335255 36.5 35.0 39.0 29.0 41.0 74-75 34.31072131287176 36.0 34.0 39.0 29.0 40.5 76-77 33.37433227807669 35.0 33.5 37.0 27.5 39.0 78-79 33.58684317371985 35.0 34.0 37.0 29.0 39.0 80-81 33.361082040289034 35.0 34.0 37.0 29.0 39.0 82-83 33.08496812935648 35.0 34.0 36.0 29.0 38.0 84-85 32.78793344784997 35.0 34.0 36.0 29.0 37.0 86-87 32.53357135759004 35.0 34.0 35.5 28.0 37.0 88-89 32.30178873099473 35.0 34.0 35.0 27.0 36.0 90-91 32.09052909527122 35.0 34.0 35.0 27.0 36.0 92-93 31.91257563066964 35.0 33.0 35.0 26.0 36.0 94-95 31.730246873269827 35.0 33.0 35.0 26.0 36.0 96-97 31.553414062832726 35.0 33.0 35.0 25.0 35.0 98-99 31.330208259394386 35.0 33.0 35.0 25.0 35.0 100-101 30.594240854048067 34.5 32.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15873290681774677 1101 2 0.1376096813722043 1101 3 0.16033848481429658 1101 4 0.11218999790350637 1101 5 0.1390246770843433 1101 6 0.1399901190665318 1101 7 0.19350376728163354 1101 8 0.13301774371742425 1101 9 0.0929107494360153 1101 10-11 0.12482759824883516 1101 12-13 0.10465721088602464 1101 14-15 0.09904897348484809 1101 16-17 0.13882214775220092 1101 18-19 0.15645225427564924 1101 20-21 0.07808454117311214 1101 22-23 -0.07579048422103085 1101 24-25 0.045563228055172544 1101 26-27 0.1530245739149052 1101 28-29 0.16248405763321472 1101 30-31 0.25300015448233637 1101 32-33 0.2620047312153204 1101 34-35 0.1063426243520027 1101 36-37 0.14265212395613958 1101 38-39 0.134533260854937 1101 40-41 -0.07545709030322456 1101 42-43 0.11963365172650953 1101 44-45 0.13894669112023905 1101 46-47 0.22231453782866595 1101 48-49 0.15532546534058866 1101 50-51 0.26674565196847055 1101 52-53 0.11654635809896519 1101 54-55 0.21664110023456828 1101 56-57 0.05517357784894017 1101 58-59 0.19819792019215754 1101 60-61 0.20672515648605838 1101 62-63 0.32967077580567405 1101 64-65 0.35747472957715587 1101 66-67 -0.008824133672511891 1101 68-69 -0.12692652934837412 1101 70-71 -0.01084728123075962 1101 72-73 -0.20937749651916704 1101 74-75 -0.20118644089522064 1101 76-77 -0.18650066626282324 1101 78-79 -0.20988041848872285 1101 80-81 -0.07313711195915573 1101 82-83 -0.1810408069588476 1101 84-85 -0.14702536822704104 1101 86-87 0.07072758644054034 1101 88-89 0.07435787052560983 1101 90-91 -0.028969535710892558 1101 92-93 -0.216380857619491 1101 94-95 -0.0745149293121905 1101 96-97 -0.031102633222051423 1101 98-99 -0.4116098890940165 1101 100-101 -0.7120251606882064 1102 1 0.17904822213306204 1102 2 0.13198805975058292 1102 3 0.1858339803097948 1102 4 0.034171979885492476 1102 5 0.13113278519244886 1102 6 0.04835047942688675 1102 7 0.07524250602037341 1102 8 0.07563035633003068 1102 9 0.036090929616193534 1102 10-11 0.1735257964470378 1102 12-13 0.1742292829581018 1102 14-15 0.3138282527641181 1102 16-17 0.18409241802247323 1102 18-19 0.24198378580717872 1102 20-21 0.18208904567761408 1102 22-23 0.17656086713031982 1102 24-25 0.1925722370641907 1102 26-27 0.2332182676085921 1102 28-29 0.16063270628186643 1102 30-31 0.04136501934720371 1102 32-33 0.156806533017118 1102 34-35 0.04986965137903354 1102 36-37 0.21553500683902627 1102 38-39 0.019902630224308382 1102 40-41 0.18352489167875774 1102 42-43 0.2515660841589451 1102 44-45 0.20574849292204078 1102 46-47 0.1236884117025383 1102 48-49 0.1378164563315849 1102 50-51 0.1049753816981962 1102 52-53 0.17472653827914542 1102 54-55 0.3456681272616038 1102 56-57 0.33273664091200317 1102 58-59 0.279941163435403 1102 60-61 0.32336930063020475 1102 62-63 0.1772158208507264 1102 64-65 0.20917743227985852 1102 66-67 0.1560542447058637 1102 68-69 0.16540229948045493 1102 70-71 0.2039500160665355 1102 72-73 0.11619908005741308 1102 74-75 0.25543518072640126 1102 76-77 0.279120955358799 1102 78-79 0.2390925544842517 1102 80-81 0.11142144659940101 1102 82-83 0.17398171556367714 1102 84-85 0.29806472186304944 1102 86-87 0.3255519107648652 1102 88-89 0.18670021286795446 1102 90-91 0.055922356181000055 1102 92-93 0.24701553877690685 1102 94-95 0.3331472328499707 1102 96-97 0.26864061002119044 1102 98-99 0.11687209288796296 1102 100-101 0.2247451095820665 1103 1 0.16749066971543414 1103 2 0.13460166693928954 1103 3 0.10459307316387623 1103 4 0.09665379417732112 1103 5 -0.037783774499459355 1103 6 0.06624692786505904 1103 7 0.05317746536996282 1103 8 0.13204456257735586 1103 9 0.06435109221781943 1103 10-11 0.1094774438582391 1103 12-13 0.027761589333799463 1103 14-15 0.03669902726134211 1103 16-17 0.05687804061554402 1103 18-19 0.14134279308146347 1103 20-21 0.20878181418424901 1103 22-23 0.18429000705329912 1103 24-25 0.09450591271673403 1103 26-27 0.0700869026107398 1103 28-29 0.05293309139097602 1103 30-31 0.14576938391716254 1103 32-33 0.04964307559307457 1103 34-35 0.009669394639622908 1103 36-37 -0.04774269962225475 1103 38-39 -0.10172424183388529 1103 40-41 -0.03374040571105752 1103 42-43 -0.05311684267032746 1103 44-45 0.06079556181378365 1103 46-47 -0.15295044152809112 1103 48-49 0.051584962964021486 1103 50-51 -0.12401563242246993 1103 52-53 -0.13749468551204558 1103 54-55 -0.0036040165124688883 1103 56-57 0.059277505268021 1103 58-59 0.2006998283904764 1103 60-61 -0.1486546617146871 1103 62-63 -0.022101680219030584 1103 64-65 0.1798821819589307 1103 66-67 0.08227889168917812 1103 68-69 0.06445578685742959 1103 70-71 0.021254252266793117 1103 72-73 0.05764623452895279 1103 74-75 0.1101335119202389 1103 76-77 0.05559592326637386 1103 78-79 0.10214903715434076 1103 80-81 0.03627724463107285 1103 82-83 0.21528894705704005 1103 84-85 0.07048235130250902 1103 86-87 0.021127007042146317 1103 88-89 0.14647128689448152 1103 90-91 0.03169642550064111 1103 92-93 0.15513321100625888 1103 94-95 0.22768253451878095 1103 96-97 0.050763844937748104 1103 98-99 0.10621398420161654 1103 100-101 0.21410197736127046 1104 1 0.12192672287005735 1104 2 0.11030265229638303 1104 3 0.1021911081261706 1104 4 0.0859807817385132 1104 5 0.15133366958916383 1104 6 0.05299314103986319 1104 7 0.17747834514439376 1104 8 0.11683482042774074 1104 9 0.08483550742204926 1104 10-11 0.13786839488881242 1104 12-13 0.15386394288650962 1104 14-15 0.19574507735877233 1104 16-17 0.15001155889424211 1104 18-19 0.17292798458587555 1104 20-21 0.3385929412345732 1104 22-23 0.17483439840005843 1104 24-25 0.21045242236635175 1104 26-27 0.22536903619145932 1104 28-29 0.19973820217573746 1104 30-31 0.23712433709830805 1104 32-33 0.21827063072189645 1104 34-35 0.17464413021668435 1104 36-37 0.19667629974279777 1104 38-39 0.09472175003900674 1104 40-41 0.19583843137132817 1104 42-43 0.2629394259150999 1104 44-45 0.12022838342530662 1104 46-47 0.05438224198259434 1104 48-49 -0.006049831307919362 1104 50-51 -0.04074034792908776 1104 52-53 0.04558419252214918 1104 54-55 0.08329225865768564 1104 56-57 0.007447527414420563 1104 58-59 0.04084893349225638 1104 60-61 0.15738909427096814 1104 62-63 0.1455807397810176 1104 64-65 0.14308962432028238 1104 66-67 0.11725639252646403 1104 68-69 0.20214182359078592 1104 70-71 0.22107572674246967 1104 72-73 0.2187520385749906 1104 74-75 0.15206815608963353 1104 76-77 0.23894281259189398 1104 78-79 0.25732818303763594 1104 80-81 0.29405484310392893 1104 82-83 0.1521234296087357 1104 84-85 0.20652545043748916 1104 86-87 0.29704969977307627 1104 88-89 0.14660650893028304 1104 90-91 0.20756859749496215 1104 92-93 0.3697291626977375 1104 94-95 0.31552941857328065 1104 96-97 0.2847164154539108 1104 98-99 0.43823111584016416 1104 100-101 0.3976402454001331 1105 1 0.09637787222514049 1105 2 0.07670015305936317 1105 3 -0.023263625583396674 1105 4 0.08011427577253727 1105 5 0.08755635793646377 1105 6 0.032885777155556184 1105 7 0.14011543419716332 1105 8 0.1463976982575943 1105 9 0.0588337166020807 1105 10-11 0.05671978048631843 1105 12-13 0.1604079201588391 1105 14-15 0.21805354435405633 1105 16-17 0.2431163590906138 1105 18-19 0.18890459612025268 1105 20-21 0.20890120037374516 1105 22-23 0.2719697069093243 1105 24-25 0.17260477236191463 1105 26-27 0.28085730996586733 1105 28-29 0.27398568356854724 1105 30-31 0.21845587263142363 1105 32-33 0.033450486362731624 1105 34-35 0.07904951632685453 1105 36-37 0.1754736194848192 1105 38-39 -0.056686688376061056 1105 40-41 -0.04899997019969504 1105 42-43 -0.0897770711510617 1105 44-45 0.03365825351136209 1105 46-47 0.0789769322673024 1105 48-49 0.1530153513592083 1105 50-51 0.21302694707573266 1105 52-53 0.21558780285864287 1105 54-55 0.11467794923827057 1105 56-57 0.017476972403713376 1105 58-59 0.22387670671348303 1105 60-61 0.18982725029257352 1105 62-63 0.07228334694034544 1105 64-65 0.12448068580962968 1105 66-67 0.20677800161194426 1105 68-69 0.10346000359340479 1105 70-71 0.10967745621386626 1105 72-73 0.0906643962022784 1105 74-75 0.20375316722118697 1105 76-77 0.0022480271819986797 1105 78-79 0.16310790132280317 1105 80-81 0.054911931559502136 1105 82-83 0.16483193041941746 1105 84-85 0.3182488839256692 1105 86-87 0.15538800652913665 1105 88-89 0.16551850629949882 1105 90-91 0.1135208828845009 1105 92-93 0.16525188009059377 1105 94-95 0.05493544647796611 1105 96-97 0.23433981198558484 1105 98-99 0.30362101860926316 1105 100-101 0.2837131881578827 1106 1 0.037360432955829026 1106 2 0.03270501810906268 1106 3 0.02904862525044649 1106 4 0.002419817342740771 1106 5 -0.004738065742976971 1106 6 -0.007696191263114827 1106 7 0.01238231692360614 1106 8 -0.0012716754543191655 1106 9 -0.011756183640692086 1106 10-11 -0.02241526169016339 1106 12-13 -0.041317890648819855 1106 14-15 0.0566801940234285 1106 16-17 -0.0018158499316598409 1106 18-19 0.09071965384178071 1106 20-21 0.08545315752633798 1106 22-23 0.03624282247129429 1106 24-25 0.1005841059034509 1106 26-27 0.07912371922799366 1106 28-29 0.058771511351267236 1106 30-31 0.08087799460368927 1106 32-33 0.15281276918690168 1106 34-35 0.12501871595356562 1106 36-37 0.1109805912284827 1106 38-39 0.09275799131865625 1106 40-41 0.209190351344823 1106 42-43 0.09623476249328178 1106 44-45 0.044178386303656225 1106 46-47 0.1427660620746991 1106 48-49 0.12832963275484133 1106 50-51 0.2589452066831157 1106 52-53 0.33659276237892044 1106 54-55 0.21945267765387655 1106 56-57 0.24157952966677954 1106 58-59 0.16481925637445016 1106 60-61 0.1500721773149749 1106 62-63 0.19500600391247502 1106 64-65 0.3524224534023688 1106 66-67 0.41022262129005327 1106 68-69 0.33202953746073405 1106 70-71 0.21703993761149576 1106 72-73 0.2525289935556998 1106 74-75 0.39800005700883645 1106 76-77 0.28035330297496586 1106 78-79 0.2378209793484629 1106 80-81 0.254240597675647 1106 82-83 0.28701832791531245 1106 84-85 0.31659620446212955 1106 86-87 0.27473658185719785 1106 88-89 0.30991586364244483 1106 90-91 0.35593684018824234 1106 92-93 0.4031195419955722 1106 94-95 0.5259468707497881 1106 96-97 0.4524016239016966 1106 98-99 0.45792741374895485 1106 100-101 0.4654493944342093 1107 1 0.24955161456028918 1107 2 0.1803347439265508 1107 3 0.09351180915636803 1107 4 0.09025427360403171 1107 5 0.04644335643342146 1107 6 0.12794951641529195 1107 7 0.14019282337646644 1107 8 0.1045382136292261 1107 9 0.1007282682023174 1107 10-11 0.1688208281826462 1107 12-13 0.16560266881933927 1107 14-15 0.27899853072428726 1107 16-17 0.22417777586883147 1107 18-19 0.2363532320755084 1107 20-21 0.25527727067214556 1107 22-23 0.2782200923437159 1107 24-25 0.2911174329494983 1107 26-27 0.33926860685132 1107 28-29 0.2680680312982773 1107 30-31 0.32110697601168425 1107 32-33 0.307087338445001 1107 34-35 0.2509582922194724 1107 36-37 0.32094975842580453 1107 38-39 0.29724909553400636 1107 40-41 0.19311933247543323 1107 42-43 0.23267179656865977 1107 44-45 0.2842262779953586 1107 46-47 0.23871686431799333 1107 48-49 0.22415898204837248 1107 50-51 0.31449081177042615 1107 52-53 0.3521708368127392 1107 54-55 0.22766353106987225 1107 56-57 0.25242628853730054 1107 58-59 0.15097005378339645 1107 60-61 0.13909703098696014 1107 62-63 0.2581886814329053 1107 64-65 0.2576495268300931 1107 66-67 0.20137466492911216 1107 68-69 0.09528980221627847 1107 70-71 0.13363484863383945 1107 72-73 0.06352759833274746 1107 74-75 0.1674312411334924 1107 76-77 0.18393907748617977 1107 78-79 0.18224882500296502 1107 80-81 0.12433586988677092 1107 82-83 0.1963706409302759 1107 84-85 0.24645167743319973 1107 86-87 0.2798926855514665 1107 88-89 0.3418096460043003 1107 90-91 0.22344435224140824 1107 92-93 0.33518078281279884 1107 94-95 0.2757545867567366 1107 96-97 0.25294001908225283 1107 98-99 0.4410686348008568 1107 100-101 0.4367825357146984 1108 1 0.05570725666845533 1108 2 -0.028831631148101167 1108 3 -0.044843371340654414 1108 4 0.029470587021535266 1108 5 0.011385790344142777 1108 6 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15 5698.0 16 7310.0 17 10676.0 18 10560.0 19 6543.0 20 4773.0 21 4710.0 22 5028.0 23 5569.0 24 6274.0 25 7301.0 26 8101.0 27 10156.0 28 12835.0 29 16535.0 30 21189.0 31 27286.0 32 35302.0 33 47126.0 34 66408.0 35 104262.0 36 182687.0 37 382901.0 38 581983.0 39 174522.0 40 182.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.72244521018264 19.934934340821147 14.39004068307583 23.952579765920383 2 16.21782768558084 21.77277437855794 38.78337899802672 23.2260189378345 3 18.404480166450313 25.60610242100254 31.073034390569937 24.91638302197721 4 13.514990182987747 18.87938961299333 37.09285808808788 30.512762115931046 5 13.825868457290499 36.85773910080777 35.08184154114791 14.234550900753822 6 30.14170724436053 36.81287886316208 18.491503527775016 14.553910364702375 7 25.545009298561915 32.893343365369596 23.28814184920714 18.273505486861342 8 24.18852656833378 34.42546244374725 21.82706094462032 19.558950043298648 9 25.2691472295964 16.272341392086993 21.6887891994719 36.7697221788447 10-11 23.612164396349968 26.44446457161726 29.492605014864925 20.450766017167847 12-13 24.578895813517697 24.856603398588888 28.826989963231643 21.737510824661772 14-15 22.082981856857675 26.6285770746387 27.018549156215123 24.269891912288507 16-17 21.39499887848132 28.737084789651362 28.03708677715282 21.830829554714494 18-19 21.15752135905212 27.590974897240038 30.090632056451327 21.160871687256517 20-21 21.95641742735055 26.943654973665904 29.880950014906094 21.218977584077457 22-23 21.765363921579763 26.9928592722988 29.503612952683806 21.738163853437626 24-25 21.546145000780793 27.146462997402082 29.792138101389813 21.515253900427307 26-27 21.516777383971135 27.243157574048855 29.907698195899922 21.33236684608009 28-29 21.35025848564636 27.278519668163888 29.957133434359523 21.414088411830228 30-31 21.805681350349936 27.04890617680044 29.761530926591046 21.38388154625857 32-33 21.686706622597598 27.135952473093063 29.62064390395699 21.55669700035235 34-35 21.758819525666986 27.301791207265346 29.534177072853673 21.405212194213995 36-37 21.702622433218245 27.29400440668726 29.541982782118843 21.46139037797565 38-39 21.557899518746186 27.27278147669681 29.756136341051377 21.41318266350563 40-41 21.891739185985433 27.20143098479579 29.504919010235515 21.401910818983264 42-43 21.847588762226543 27.234593489587027 29.278176062236483 21.639641685949947 44-45 21.931999829644667 27.398503712326626 29.04845189591289 21.621044562115813 46-47 21.846254312119363 27.346062662369924 28.94964580287049 21.858037222640224 48-49 21.843982907681607 27.49058076972218 28.903223974673843 21.762212347922375 50-51 21.910620235374285 27.69478002867648 28.685282718871115 21.70931701707812 52-53 22.004315668431737 27.811047543334137 28.43957354381681 21.745063244417313 54-55 22.034099459121816 27.689328658025865 28.419812325208333 21.856759557643983 56-57 21.921614378448453 27.905489105915336 28.349094518560076 21.823801997076135 58-59 21.774718813049503 27.979258576152304 28.58547078194021 21.660551828857987 60-61 21.99034238343299 27.83431625386465 28.555840779650804 21.619500583051558 62-63 21.818338249963585 28.01525163407323 28.35517066797739 21.811239447985802 64-65 21.903370098700332 27.902874921847804 28.392401533912516 21.80135344553935 66-67 21.770679593015366 28.071763495974615 28.230967244403733 21.926589666606283 68-69 21.780771198649838 27.972972896234072 28.32936677620838 21.916889128907712 70-71 21.794207117113178 27.822663304227145 28.412377351119204 21.97075222754047 72-73 21.877477426316087 27.852518598443975 28.215060480436136 22.054943494803794 74-75 21.822861726519633 27.877336324206908 28.185472020898256 22.114329928375202 76-77 21.748168077992407 27.979371854045905 28.12485803456234 22.14760203339936 78-79 21.76096439672962 27.932110858605647 28.08693607901793 22.219988665646802 80-81 21.768684303705527 27.995114988302834 28.099891163393192 22.136309544598447 82-83 21.832152323945362 27.97840818580773 28.04383104206627 22.14560844818064 84-85 21.907219934213234 27.95085725805192 27.876410603679457 22.265512204055383 86-87 21.793923906871097 27.95800681431005 27.958773424190802 22.28929585462805 88-89 21.838192454591606 27.8564443975019 27.925154654864404 22.38020849304209 90-91 21.855538677758695 27.830581621498844 28.00812027086498 22.305759429877487 92-93 21.916298746468676 27.7750457829957 28.019931573941314 22.288723896594313 94-95 21.83503925270794 27.77754432787723 27.949773569370112 22.437642850044718 96-97 21.80076943825329 27.722973836260135 27.975866327848838 22.50039039763774 98-99 21.780026848015698 27.746441842927748 27.863504415612734 22.610026893443816 100-101 21.769356462138862 27.811834986506305 27.753658062217223 22.66515048913761 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1998.0 1 1199.5 2 440.0 3 429.5 4 315.5 5 304.0 6 493.5 7 928.0 8 1474.5 9 1759.0 10 1522.5 11 1212.0 12 1267.5 13 1389.0 14 1432.5 15 1564.5 16 1743.5 17 1923.5 18 2148.5 19 2340.5 20 2589.0 21 2978.0 22 3581.5 23 4497.5 24 5619.5 25 7111.0 26 9294.5 27 12164.0 28 15378.0 29 19076.0 30 23060.5 31 27080.0 32 31626.5 33 35644.0 34 39699.0 35 43734.0 36 47688.5 37 51931.0 38 55020.0 39 57584.0 40 60188.0 41 62501.5 42 65107.5 43 68446.5 44 70729.5 45 71450.0 46 71789.0 47 71455.0 48 70624.0 49 69140.0 50 66028.0 51 61850.5 52 58154.0 53 54563.0 54 50160.0 55 45497.0 56 40338.5 57 34914.5 58 30746.5 59 26493.5 60 21281.0 61 16985.0 62 13690.0 63 10486.0 64 7572.0 65 5549.0 66 4163.5 67 3150.5 68 2379.0 69 1675.0 70 1133.5 71 825.5 72 570.5 73 378.0 74 274.0 75 180.0 76 112.5 77 72.5 78 46.0 79 28.0 80 15.0 81 12.5 82 7.5 83 5.0 84 5.0 85 1.5 86 3.0 87 3.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019420507942817394 2 0.0 3 0.0010789171079342997 4 0.017376243948836616 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.8392555471955252E-5 12-13 0.0 14-15 8.517766641586576E-5 16-17 0.00113570221887821 18-19 1.9874788830368678E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.8267344302323933E-4 28-29 0.005422978095143453 30-31 0.0 32-33 8.517766641586576E-5 34-35 0.009852216748768473 36-37 0.003691032211354183 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.8392555471955252E-5 58-59 4.5428088755128404E-4 60-61 0.0017887309947331811 62-63 0.009341150750273279 64-65 0.0021578342158685994 66-67 0.004202098209849378 68-69 0.0029528257690833466 70-71 1.1357022188782101E-4 72-73 0.007098138867988814 74-75 0.006388324981189931 76-77 0.0018739086611490466 78-79 3.9749577660737356E-4 80-81 0.007296886756292501 82-83 0.009795431637824563 84-85 0.0018455161056770916 86-87 0.0014196277735977626 88-89 3.9749577660737356E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 5.6785110943910504E-5 100-101 9.937394415184338E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1761025.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.182365506568814 #Duplication Level Percentage of deduplicated Percentage of total 1 65.27336236078078 37.324852643568775 2 19.82097521800359 22.66820499225047 3 7.441193402378272 12.765151228195885 4 3.3023882496547587 7.553534877454256 5 1.6345325222825235 4.673321806076655 6 0.8832571716461248 3.0304040655220135 7 0.4685251303208011 1.8753962675711784 8 0.3006867165885717 1.375518218475022 9 0.21614655866696889 1.112379436861347 >10 0.6436050240631686 5.488180273609312 >50 0.01085580099622688 0.4026113682907485 >100 0.003478205082173823 0.4487295093712958 >500 2.9809186082642273E-4 0.12562605534608284 >1k 4.968197680440379E-4 0.4936973292185046 >5k 1.9872790721761518E-4 0.6623919281884367 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6133 0.34826308541900314 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5525 0.31373773796510557 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3032 0.17217245638193665 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.1357022188782101E-4 7 0.0 0.0 0.0 0.0 1.1357022188782101E-4 8 0.0 0.0 0.0 0.0 1.1357022188782101E-4 9 0.0 0.0 0.0 0.0 1.1357022188782101E-4 10-11 0.0 0.0 0.0 0.0 1.1357022188782101E-4 12-13 0.0 0.0 0.0 0.0 2.8392555471955255E-4 14-15 0.0 0.0 0.0 0.0 3.9749577660737356E-4 16-17 0.0 0.0 0.0 0.0 5.110659984951945E-4 18-19 0.0 0.0 0.0 0.0 5.678511094391051E-4 20-21 0.0 0.0 0.0 8.517766641586576E-5 5.678511094391051E-4 22-23 0.0 0.0 0.0 2.839255547195525E-4 6.81421331326926E-4 24-25 0.0 0.0 0.0 7.098138867988814E-4 6.81421331326926E-4 26-27 0.0 0.0 0.0 9.653468860464785E-4 8.517766641586576E-4 28-29 0.0 0.0 0.0 0.0014196277735977628 8.517766641586576E-4 30-31 0.0 0.0 0.0 0.002839255547195525 8.517766641586576E-4 32-33 0.0 0.0 0.0 0.005309407873255632 8.517766641586576E-4 34-35 0.0 0.0 0.0 0.00854615919705853 8.517766641586576E-4 36-37 0.0 0.0 0.0 0.0116125551880297 8.517766641586576E-4 38-39 0.0 0.0 0.0 0.014224670291449582 8.517766641586576E-4 40-41 0.0 0.0 0.0 0.01609857895259863 8.517766641586576E-4 42-43 0.0 0.0 0.0 0.02032906971791996 8.517766641586576E-4 44-45 0.0 0.0 0.0 0.02572365525759146 8.517766641586576E-4 46-47 0.0 0.0 0.0 0.03168609190670206 8.801692196306128E-4 48-49 0.0 0.0 0.0 0.04574040686531991 9.085617751025681E-4 50-51 0.0 0.0 0.0 0.05562101616956034 9.085617751025681E-4 52-53 0.0 0.0 0.0 0.07169120256668701 9.085617751025681E-4 54-55 0.0 0.0 0.0 0.09639272582728808 9.653468860464787E-4 56-57 0.0 0.0 0.0 0.12313851308186993 9.653468860464787E-4 58-59 0.0 0.0 0.0 0.1508212546670263 9.653468860464787E-4 60-61 0.0 0.0 0.0 0.18409732968015785 9.653468860464787E-4 62-63 0.0 0.0 0.0 0.22322227112051218 9.653468860464787E-4 64-65 0.0 0.0 0.0 0.2680825087662015 9.653468860464787E-4 66-67 0.0 0.0 0.0 0.33145469257960564 9.653468860464787E-4 68-69 0.0 0.0 0.0 0.41101063301202423 9.653468860464787E-4 70-71 0.0 0.0 0.0 0.486676793344785 9.653468860464787E-4 72-73 0.0 0.0 0.0 0.5698953734330858 9.937394415184338E-4 74-75 0.0 0.0 0.0 0.6641018724890333 0.0010505245524623443 76-77 0.0 0.0 0.0 0.767166848852231 0.0010789171079342997 78-79 0.0 0.0 0.0 0.880084041964197 0.0010789171079342997 80-81 0.0 0.0 0.0 0.995499779957695 0.0010789171079342997 82-83 0.0 0.0 0.0 1.1266733862381284 0.0011357022188782102 84-85 0.0 0.0 0.0 1.288766485427521 0.0011357022188782102 86-87 0.0 0.0 0.0 1.4872020556210162 0.0011640947743501654 88-89 0.0 0.0 0.0 1.7008276429920075 0.0011924873298221207 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2935 0.0 57.629997 1 GTATCAA 5030 0.0 41.655975 1 TCAACGC 5960 0.0 34.669777 4 ATCAACG 6020 0.0 34.245323 3 CAACGCA 6175 0.0 33.305016 5 TATCAAC 6325 0.0 32.593967 2 AACGCAG 6435 0.0 32.180782 6 ACGCAGA 7135 0.0 28.757322 7 CGCAGAG 7305 0.0 27.958054 8 GTGGTAT 1170 0.0 27.20794 1 GCAGAGT 8350 0.0 24.06094 9 GAGTACT 5770 0.0 23.049063 12-13 CAGAGTA 7965 0.0 22.481567 10-11 TGGTATC 1415 0.0 21.48481 2 GTACATG 3435 0.0 20.194487 1 GTACTTT 6575 0.0 19.974247 14-15 AGAGTAC 7425 0.0 19.63871 10-11 TACATGG 3635 0.0 18.687002 2 AGTACTT 6300 0.0 17.64194 12-13 TATGCCG 665 0.0 17.498594 42-43 >>END_MODULE