FastQCFastQC Report
Fri 10 Feb 2017
SRR2031706_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031706_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929791
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32190.34620683572975003No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30700.33018172901221887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14110.15175453408346606No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13020.14003146943775538No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9630.10357166287907713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21850.057.4060171
GTATCAA34000.036.7520642
GTGGTAT8900.036.3014371
TGGTATC8900.036.3014372
TCAACGC40500.031.555775
ATCAACG40400.031.1651674
CAACGCA40950.030.7349936
TATCAAC41450.030.2610743
AACGCAG43400.028.8905227
GTTGCGA350.00839340927.14250434-35
ACGCAGA47150.026.192668
CGCAGAG49000.025.3006929
GAGTACT32400.023.6088112-13
CGTCAGA2653.092282E-1123.307862
GCGTCAG2503.8198777E-1022.8058431
CAGAGTA50850.021.76764310-11
GTACTTT35150.021.62716314-15
TGCACCG1150.00371838220.6563555
GCAGAGT55050.020.1904729
GTACATG28200.019.3755321
Produced by FastQC (version 0.11.5)