##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031706_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 929791 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82270316662562 33.0 31.0 34.0 30.0 34.0 2 32.133332114421414 34.0 31.0 34.0 30.0 34.0 3 32.23288029245282 34.0 31.0 34.0 30.0 34.0 4 35.67880201034426 37.0 35.0 37.0 35.0 37.0 5 35.67981191472062 37.0 35.0 37.0 35.0 37.0 6 35.710430623656286 37.0 37.0 37.0 35.0 37.0 7 35.684952854996446 37.0 36.0 37.0 35.0 37.0 8 35.6798409535046 37.0 36.0 37.0 35.0 37.0 9 37.430434366432884 39.0 38.0 39.0 35.0 39.0 10-11 37.358789771034566 39.0 38.0 39.0 35.0 39.0 12-13 37.305392287083876 39.0 38.0 39.0 35.0 39.0 14-15 38.64719867152941 41.0 38.5 41.0 34.5 41.0 16-17 38.570499714451955 41.0 38.0 41.0 34.0 41.0 18-19 38.5914641032232 41.0 38.5 41.0 34.0 41.0 20-21 38.60349153734549 41.0 39.0 41.0 34.0 41.0 22-23 38.50249733542269 40.5 38.5 41.0 34.0 41.0 24-25 38.42653994284737 40.5 38.0 41.0 34.0 41.0 26-27 38.293650938759356 40.0 38.0 41.0 34.0 41.0 28-29 38.17876920727346 40.0 38.0 41.0 34.0 41.0 30-31 37.99728810022897 40.0 38.0 41.0 33.5 41.0 32-33 37.76222774795626 40.0 38.0 41.0 33.0 41.0 34-35 37.57598750686982 40.0 37.5 41.0 33.0 41.0 36-37 37.383806683437456 40.0 37.0 41.0 32.0 41.0 38-39 37.18594985324659 40.0 37.0 41.0 31.5 41.0 40-41 37.06735330843168 40.0 37.0 41.0 31.0 41.0 42-43 37.01131598391467 40.0 37.0 41.0 31.0 41.0 44-45 36.84431339946289 40.0 37.0 41.0 30.5 41.0 46-47 36.707326700301465 40.0 37.0 41.0 30.0 41.0 48-49 36.697337358610696 40.0 37.0 41.0 30.0 41.0 50-51 36.12058408825209 39.5 36.0 40.5 29.5 41.0 52-53 36.12288406749474 39.5 36.0 40.5 30.0 41.0 54-55 36.32848941321221 40.0 36.0 41.0 30.0 41.0 56-57 36.19292023691346 40.0 36.0 41.0 29.0 41.0 58-59 35.98990794705477 39.5 35.0 41.0 28.5 41.0 60-61 35.74039488444177 39.0 35.0 41.0 28.0 41.0 62-63 35.45244200040654 39.0 35.0 41.0 28.0 41.0 64-65 35.170997030515466 38.5 35.0 41.0 28.0 41.0 66-67 34.87049670302251 37.5 35.0 40.0 27.0 41.0 68-69 34.52636775361344 37.0 35.0 40.0 26.0 41.0 70-71 34.16382391311596 36.5 34.0 39.0 26.0 41.0 72-73 33.78910583131048 36.0 34.0 39.0 26.0 41.0 74-75 33.38510697565367 35.5 34.0 39.0 25.5 40.5 76-77 33.00776787471593 35.0 34.0 37.5 25.0 39.5 78-79 32.6266101736842 35.0 34.0 37.0 24.5 39.0 80-81 32.28779585949961 35.0 33.5 36.5 24.0 39.0 82-83 31.974605583405307 35.0 33.0 36.0 23.0 38.0 84-85 31.66465904703315 35.0 33.0 36.0 21.5 37.0 86-87 31.388919660439818 35.0 33.0 35.0 20.0 37.0 88-89 31.12775344136478 35.0 33.0 35.0 19.5 36.0 90-91 30.891740724528418 35.0 32.5 35.0 18.0 36.0 92-93 30.6765821566352 35.0 32.0 35.0 15.0 36.0 94-95 30.423823741034276 35.0 32.0 35.0 7.0 36.0 96-97 30.148446263730236 35.0 32.0 35.0 2.0 35.0 98-99 29.88927565442126 35.0 32.0 35.0 2.0 35.0 100-101 29.285573317014254 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1546874729743628 1101 2 -0.012879128412336627 1101 3 0.004802907192292594 1101 4 0.12239232440837355 1101 5 0.0752542230473523 1101 6 0.17901032022662378 1101 7 0.18083673720406068 1101 8 0.2019532140985163 1101 9 0.20611523738370607 1101 10-11 0.17395687055090292 1101 12-13 0.1469283578236329 1101 14-15 0.09134585027263853 1101 16-17 0.23005686600085795 1101 18-19 0.3433140090768063 1101 20-21 0.32253333583295785 1101 22-23 0.42388510676046565 1101 24-25 0.0028741146441007004 1101 26-27 -0.10741191820625318 1101 28-29 0.029618483843002252 1101 30-31 0.16419197674991892 1101 32-33 0.23494171517832996 1101 34-35 0.25125540925440504 1101 36-37 -0.02613103056893351 1101 38-39 0.21003600065921546 1101 40-41 -0.09520507750654872 1101 42-43 -0.12122838356849286 1101 44-45 -0.21145733132329525 1101 46-47 -0.1824848045961147 1101 48-49 -0.11327117972033562 1101 50-51 0.043005483457214666 1101 52-53 0.03133258340264433 1101 54-55 0.2761464763049375 1101 56-57 0.2770411836866131 1101 58-59 -0.06019881203875599 1101 60-61 -0.44185048821744743 1101 62-63 -0.22387591679299845 1101 64-65 0.28264184619970933 1101 66-67 -0.23673578626225122 1101 68-69 -0.40294978199755604 1101 70-71 -0.3815675330574493 1101 72-73 -0.1548944336782938 1101 74-75 0.048672585724283124 1101 76-77 -0.18164033561363624 1101 78-79 -0.5650316163842319 1101 80-81 -0.6087457089158193 1101 82-83 -0.3928852693426528 1101 84-85 -0.24505359617446842 1101 86-87 -0.23279340560724648 1101 88-89 -0.4532529050158445 1101 90-91 -0.8814170666040688 1101 92-93 -0.6277973817982705 1101 94-95 -0.5992168226494243 1101 96-97 -0.6471780634600393 1101 98-99 -0.45349476255222854 1101 100-101 -0.704245397909883 1102 1 -0.08293444085649426 1102 2 0.10743033415172931 1102 3 0.044670429986354065 1102 4 0.10255019744232641 1102 5 -0.0036561126619290008 1102 6 0.09841129286066064 1102 7 0.09524690600777319 1102 8 -0.14751432440430534 1102 9 0.15914931884671546 1102 10-11 0.12536934985133996 1102 12-13 0.11854295649754931 1102 14-15 0.2584968703602186 1102 16-17 0.02357667540124453 1102 18-19 -0.0015194453448827971 1102 20-21 -0.13530991692752536 1102 22-23 0.014573739509302186 1102 24-25 -0.11400887473285337 1102 26-27 -0.07841125712928232 1102 28-29 -0.05589443988443321 1102 30-31 -0.1931463048426849 1102 32-33 -0.021651054865067465 1102 34-35 0.22446051983752824 1102 36-37 0.05148660513184211 1102 38-39 -0.1877637911997212 1102 40-41 0.0050364446722568346 1102 42-43 0.07734179204234692 1102 44-45 -0.2136369217173879 1102 46-47 -0.005749804825192939 1102 48-49 -0.05087795019571928 1102 50-51 -0.08654596600312203 1102 52-53 0.043793229687196344 1102 54-55 0.1439294467004757 1102 56-57 -0.048560494532537746 1102 58-59 0.06491000384803414 1102 60-61 -0.19896197459336662 1102 62-63 -0.06283167699878334 1102 64-65 0.1687467938044449 1102 66-67 0.06476603660839686 1102 68-69 0.05090082769867621 1102 70-71 0.09392680197112213 1102 72-73 -0.03110613539512741 1102 74-75 -0.4175355815216406 1102 76-77 0.11372198038115044 1102 78-79 0.021178383550314095 1102 80-81 -0.11675717631032256 1102 82-83 -0.10723620303205195 1102 84-85 -0.12716590071495304 1102 86-87 0.03904995187830451 1102 88-89 -0.1034122834725899 1102 90-91 -0.23808608958467303 1102 92-93 -0.03751946765973457 1102 94-95 -0.24810640970540376 1102 96-97 -0.5511631794103167 1102 98-99 -0.2942275761874349 1102 100-101 -0.07232454387698439 1103 1 -0.1829509437169925 1103 2 0.051531478350057114 1103 3 0.1904212490172128 1103 4 0.11818512971947825 1103 5 0.13256277638994618 1103 6 0.10123511565660692 1103 7 0.02873120995378997 1103 8 0.11743298868551477 1103 9 0.2833211197366481 1103 10-11 0.18764472758348205 1103 12-13 0.2706932180984509 1103 14-15 0.3354934108716847 1103 16-17 0.284369240556984 1103 18-19 0.2378591302748987 1103 20-21 0.013241804259699563 1103 22-23 0.0407216051393533 1103 24-25 0.1581446874401493 1103 26-27 0.10689447364183025 1103 28-29 0.018270637617895602 1103 30-31 0.15348099944561255 1103 32-33 -0.10827273595646147 1103 34-35 -0.10951153531870261 1103 36-37 -0.23926017986644865 1103 38-39 -0.20457531630851378 1103 40-41 -0.07065667546856957 1103 42-43 -0.22224930374758856 1103 44-45 -0.36006007950917507 1103 46-47 -0.280982372914778 1103 48-49 -0.2526055552028126 1103 50-51 -0.20106970567355376 1103 52-53 -0.10057165467053153 1103 54-55 -0.0877951633430385 1103 56-57 -0.23154207245048752 1103 58-59 -0.08166901429288487 1103 60-61 -0.22941128025079394 1103 62-63 -0.22606635989937018 1103 64-65 -0.23710591510953805 1103 66-67 -0.35338894358816475 1103 68-69 -0.26317152817633627 1103 70-71 -0.39594533142088295 1103 72-73 -0.4688949354538039 1103 74-75 -0.3347080303016483 1103 76-77 -0.31489550776123565 1103 78-79 -0.5215701373044155 1103 80-81 -0.6502579831168411 1103 82-83 -0.2739548231484292 1103 84-85 -0.2855719688778784 1103 86-87 -0.22902526449253102 1103 88-89 -0.47166903130888116 1103 90-91 -0.331895683247577 1103 92-93 -0.7769163797911673 1103 94-95 -0.4545510823375487 1103 96-97 -0.21582963826318746 1103 98-99 -0.10258963672237442 1103 100-101 -0.23447737258951662 1104 1 -0.12866053823440993 1104 2 0.1066071493951739 1104 3 0.20196227319387106 1104 4 0.07747030009830524 1104 5 0.061305875571370905 1104 6 0.034844481733138366 1104 7 0.01063440812625771 1104 8 -0.0167641367666036 1104 9 -0.010308710836355317 1104 10-11 0.21664601256349414 1104 12-13 0.2077767893910405 1104 14-15 0.23095195836835813 1104 16-17 0.14838147166301496 1104 18-19 -0.010766654990661095 1104 20-21 0.13816446749975597 1104 22-23 0.09340578560765778 1104 24-25 -0.035411375761832176 1104 26-27 -0.05131274128297747 1104 28-29 -0.2289690349501612 1104 30-31 -0.0442512487934863 1104 32-33 -0.07236468341505287 1104 34-35 0.005886808484845574 1104 36-37 0.06323363319275188 1104 38-39 -0.1881799231998187 1104 40-41 0.13308404412023833 1104 42-43 0.07575897723470604 1104 44-45 -0.17615326435593914 1104 46-47 -0.10751602035433194 1104 48-49 -0.01580634522755986 1104 50-51 -0.09564185492160249 1104 52-53 -0.12356446830084167 1104 54-55 -0.11109137503160582 1104 56-57 -0.17979188208308017 1104 58-59 -0.07919423797458336 1104 60-61 0.12223896722684202 1104 62-63 0.1423183726344135 1104 64-65 0.05996837577185943 1104 66-67 -0.030823266832030072 1104 68-69 0.12126950551868987 1104 70-71 0.15233660271199057 1104 72-73 0.20934250029719692 1104 74-75 0.2215871819580144 1104 76-77 0.14583526513044376 1104 78-79 -0.09562305944414362 1104 80-81 -0.1725309699814872 1104 82-83 0.03449986940820793 1104 84-85 0.1875267366237381 1104 86-87 0.108442611007181 1104 88-89 0.029740877314761605 1104 90-91 0.1484369837998294 1104 92-93 -0.07355044603902883 1104 94-95 -0.05532682952962986 1104 96-97 0.0010999733872978368 1104 98-99 -0.1334886468118448 1104 100-101 -0.13083421629787395 1105 1 -0.13916124473880842 1105 2 0.1873537650112027 1105 3 0.14147068287525855 1105 4 0.1821867867203082 1105 5 0.22502687470532834 1105 6 0.30760076050609797 1105 7 0.3226244714837492 1105 8 0.47118540514505725 1105 9 0.3528288524071854 1105 10-11 0.31767614155752 1105 12-13 0.28501937109485453 1105 14-15 0.42101018800507717 1105 16-17 0.38326284729544824 1105 18-19 0.4386755483914442 1105 20-21 0.29635593019176554 1105 22-23 0.18961003375022756 1105 24-25 0.32544998984280227 1105 26-27 0.5298611955418551 1105 28-29 0.2949552551268724 1105 30-31 0.45871436039562496 1105 32-33 0.6806322626627122 1105 34-35 0.45374792339436 1105 36-37 0.6555720487996695 1105 38-39 0.5384342114462513 1105 40-41 0.7086515266855287 1105 42-43 0.7008900224054102 1105 44-45 0.8907339000544781 1105 46-47 0.9408054619374724 1105 48-49 0.8243327905580244 1105 50-51 0.6594660579276592 1105 52-53 0.854231921689582 1105 54-55 0.6646903378997067 1105 56-57 0.7744904461907751 1105 58-59 0.8167188619843913 1105 60-61 0.8497191395227475 1105 62-63 0.9449671943674005 1105 64-65 0.9563230409799388 1105 66-67 0.7981058092425428 1105 68-69 0.80667278278748 1105 70-71 0.7907089077395639 1105 72-73 0.631051557227778 1105 74-75 0.7676894427720882 1105 76-77 0.9094468700349552 1105 78-79 0.7195234832423907 1105 80-81 0.7310938533924158 1105 82-83 0.6993432989173058 1105 84-85 0.6795101907465302 1105 86-87 0.8332762406165521 1105 88-89 0.8898074254710124 1105 90-91 0.7189535296770337 1105 92-93 0.9937184586512515 1105 94-95 1.011204233873439 1105 96-97 0.9187067467133403 1105 98-99 1.0193897888506775 1105 100-101 1.1649461292055676 1106 1 0.046187518342172496 1106 2 -0.0402025973285447 1106 3 0.08210991527773359 1106 4 0.135000716713769 1106 5 0.1550122337333022 1106 6 0.11685482729269836 1106 7 0.031993072811701495 1106 8 0.08224068967155063 1106 9 0.1781137030530573 1106 10-11 0.04438294933938636 1106 12-13 0.143436849498066 1106 14-15 0.1957445164923044 1106 16-17 0.18974066703321313 1106 18-19 0.1394722928011305 1106 20-21 0.1179055771336408 1106 22-23 0.3112658412457563 1106 24-25 0.3600318577282877 1106 26-27 0.17734535249739736 1106 28-29 0.08279149774560324 1106 30-31 0.08100361954083724 1106 32-33 0.2152566758082486 1106 34-35 0.2979505576888144 1106 36-37 0.12811752495541384 1106 38-39 0.21437169287489866 1106 40-41 -2.072068433776053E-4 1106 42-43 0.0025230850698463314 1106 44-45 0.12297766953750511 1106 46-47 0.36791327567837584 1106 48-49 0.27594667408936147 1106 50-51 0.11132170658755314 1106 52-53 0.10373758628706753 1106 54-55 0.12733830085966957 1106 56-57 -0.018145306954878038 1106 58-59 0.13360406271697656 1106 60-61 0.08463785781639643 1106 62-63 0.07551563653556315 1106 64-65 0.04185387076107361 1106 66-67 -0.05576405358613812 1106 68-69 0.04556104137622441 1106 70-71 0.13549434456458442 1106 72-73 0.03750398487382256 1106 74-75 0.03403830309937206 1106 76-77 -0.02180944422190123 1106 78-79 0.158228594616439 1106 80-81 0.2393125171200765 1106 82-83 0.21724674954048595 1106 84-85 0.22020574496945144 1106 86-87 0.3124587773405416 1106 88-89 0.2845268158447105 1106 90-91 0.274345432301061 1106 92-93 0.36439504984397786 1106 94-95 0.5888783722981579 1106 96-97 0.5392408450509691 1106 98-99 0.6485004713576821 1106 100-101 0.8754627772333592 1107 1 0.07046524576765378 1107 2 0.3082383646443958 1107 3 0.416599330869424 1107 4 0.3577119138844722 1107 5 0.15277329422540475 1107 6 0.3211540566843496 1107 7 0.2834395092026867 1107 8 0.2976575121232088 1107 9 0.2714334000470018 1107 10-11 0.4308611513211176 1107 12-13 0.422570340628873 1107 14-15 0.5452838535974465 1107 16-17 0.48796406344188625 1107 18-19 0.44767126309263006 1107 20-21 0.2873557640028892 1107 22-23 0.4267370732735074 1107 24-25 0.5444839576615124 1107 26-27 0.24660190528705073 1107 28-29 0.40688271961883515 1107 30-31 0.2526883815134795 1107 32-33 0.4864324027361846 1107 34-35 0.47610700413247997 1107 36-37 0.3332947270520705 1107 38-39 0.3864099716119611 1107 40-41 0.25155273927077815 1107 42-43 0.3879007297171242 1107 44-45 0.13093543374036898 1107 46-47 -0.03175779370256038 1107 48-49 0.21846528463755277 1107 50-51 0.3815292631175069 1107 52-53 0.227992182368709 1107 54-55 0.4044459809022598 1107 56-57 0.5409683697794989 1107 58-59 0.3681602536975035 1107 60-61 0.33222034142442425 1107 62-63 0.40932784202918526 1107 64-65 0.32369838448640564 1107 66-67 0.09432708972781256 1107 68-69 0.24496922812618038 1107 70-71 0.32709388426538766 1107 72-73 0.22102428444654976 1107 74-75 0.1481172900919958 1107 76-77 0.16589559186445513 1107 78-79 0.2972494259191052 1107 80-81 0.5118506656523962 1107 82-83 0.4393563862911023 1107 84-85 0.29141391397083183 1107 86-87 0.21845605104253352 1107 88-89 0.19227785275447928 1107 90-91 0.34156308078901176 1107 92-93 0.6500213200675375 1107 94-95 0.6612802059299661 1107 96-97 0.6219943187532451 1107 98-99 0.5847554340492991 1107 100-101 0.7084668404939478 1108 1 0.03300244201846425 1108 2 -0.014858412765207163 1108 3 0.17473819594932394 1108 4 0.17359929976866084 1108 5 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-0.6149429143643275 2316 78-79 -0.6799199941010627 2316 80-81 -0.7848651967888287 2316 82-83 -0.7322547161393302 2316 84-85 -0.5770714338324012 2316 86-87 -0.6717803159513203 2316 88-89 -0.5887879294299267 2316 90-91 -0.7712545728708413 2316 92-93 -0.6875687025180142 2316 94-95 -0.8856167670383961 2316 96-97 -0.8406791567222562 2316 98-99 -0.7207815764196006 2316 100-101 -0.7209497705374588 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5594.0 3 751.0 4 628.0 5 631.0 6 699.0 7 917.0 8 1057.0 9 1283.0 10 1654.0 11 1917.0 12 2352.0 13 3090.0 14 3521.0 15 4265.0 16 4729.0 17 6644.0 18 6468.0 19 4018.0 20 3682.0 21 3722.0 22 3835.0 23 4249.0 24 4890.0 25 5579.0 26 6495.0 27 7621.0 28 8915.0 29 10445.0 30 13158.0 31 16322.0 32 20491.0 33 26932.0 34 38816.0 35 60196.0 36 102379.0 37 194472.0 38 264194.0 39 83061.0 40 119.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.78679731776089 19.47068212709402 14.09123317946144 23.651287375683648 2 16.723033376272557 21.620043858326405 38.14220351469771 23.51471925070333 3 18.69554353609178 25.56742423752873 30.886898569080685 24.85013365729881 4 13.9958167107542 19.073170941864774 36.505122482917876 30.42588986446315 5 14.526592617002992 36.13905742833573 34.409401162652955 14.924948792008323 6 29.825258440709646 36.16296435074074 18.672657559733963 15.339119648815657 7 25.575277651553492 33.15989757527335 23.094665342926856 18.170159430246304 8 24.675275735689954 33.71156819424885 21.58081284426586 20.032343225795337 9 25.493265576382683 16.500360676564636 21.747074213240598 36.25929953381208 10-11 23.95136964623662 26.29859644363491 28.90418612462134 20.845847785507136 12-13 25.109595228468972 25.118908736770646 28.02779193945466 21.74370409530572 14-15 22.67457043581831 26.810695611017387 26.518208050623176 23.996525902541123 16-17 21.6639427025135 28.879171771294715 27.667685348483424 21.78920017770837 18-19 21.39643860283981 27.5390625 29.804407482078854 21.260091415081334 20-21 22.489871828283068 26.61733064253854 29.628159871117532 21.264637658060863 22-23 22.394304469460234 26.77928796176849 29.12143114041983 21.70497642835145 24-25 21.987522603102178 27.07349648659372 29.43331687840056 21.50566403190355 26-27 21.731546731412145 27.059966730012437 29.71828180433156 21.49020473424386 28-29 21.6313517232691 27.115276007858174 29.814060187495727 21.439312081377 30-31 21.910481025196557 26.96868800788116 29.5562811054078 21.564549861514486 32-33 21.837523053419336 27.138275297393143 29.29353449157588 21.730667157611638 34-35 22.133051355742943 27.1529775089132 29.29284499811022 21.42112613723364 36-37 22.034195802087325 27.127600005168784 29.3801122487218 21.458091944022087 38-39 21.752195924484745 27.36679637282157 29.43475536356843 21.446252339125248 40-41 22.00913276424935 27.168896918849544 29.303177194146073 21.518793122755035 42-43 22.005848444326727 27.292650469318275 28.94290876125384 21.75859232510115 44-45 22.097021952941137 27.436465260424995 28.831723192542256 21.63478959409161 46-47 22.009850689052897 27.513282493382977 28.747741383877518 21.7291254336866 48-49 22.053799078668565 27.6881904264456 28.59713692622079 21.66087356866504 50-51 22.026407999500357 27.95076335960063 28.382515414716718 21.640313226182297 52-53 22.128833596218755 28.03029732072201 28.048169940621985 21.792699142437243 54-55 22.068416094738257 27.8805544341273 28.155241555645272 21.89578791548917 56-57 21.967162453038842 28.03919056135881 28.252124221890334 21.741522763712013 58-59 21.972113805819493 27.98686441818524 28.37414874431375 21.666873031681515 60-61 21.92615681112843 28.040762603057196 28.44278996654258 21.590290619271794 62-63 22.0140118089381 27.970484114781847 28.30161457727705 21.713889499003002 64-65 21.984648975207367 27.984094378380416 28.296553153798314 21.734703492613903 66-67 21.875156473016112 28.141022500348615 28.194970358290227 21.788850668345045 68-69 21.74043904295936 28.231535907330503 28.33550588041461 21.69251916929553 70-71 21.769091292794172 28.005605395650917 28.32382949949825 21.90147381205666 72-73 21.85578032939393 28.084030338007786 28.20898511525342 21.851204217344865 74-75 21.902127428230315 28.219226895713867 28.001548081772736 21.87709759428308 76-77 21.93698968672519 28.28825481945712 27.9349491066405 21.839806387177187 78-79 21.741829537500674 28.316319388359446 27.987616432455713 21.954234641684163 80-81 21.73971098860333 28.269738227111763 28.01549779060646 21.97505299367845 82-83 21.820328767093784 28.251742829589578 28.07766178956171 21.850266613754933 84-85 21.915443698226415 28.312244328796805 27.836947591374024 21.935364381602763 86-87 21.78243997572699 28.310145288058898 27.917781916609187 21.989632819604925 88-89 21.704842907273353 28.309693244135204 27.915655865767796 22.069807982823654 90-91 21.700123136463954 28.238685524977537 28.083943855802733 21.97724748275578 92-93 21.745508042094926 28.293990278082177 28.008536160483267 21.951965519339637 94-95 21.712530323635153 28.24542305576103 27.92961578756389 22.112430833039923 96-97 21.68556599477429 28.23261407152139 27.99124526709364 22.090574666610685 98-99 21.57766789078338 28.24145998258029 27.944851227292745 22.236020899343583 100-101 21.583572627220693 28.289115126030506 27.951231439016137 22.176080807732657 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2427.0 1 1380.0 2 318.5 3 282.5 4 231.0 5 227.0 6 353.5 7 668.5 8 1062.0 9 1273.5 10 1139.5 11 928.5 12 895.5 13 985.0 14 1057.0 15 1072.0 16 1169.5 17 1256.5 18 1320.0 19 1447.0 20 1644.5 21 1917.0 22 2331.0 23 2935.0 24 3588.5 25 4471.5 26 5720.5 27 7366.5 28 9143.5 29 10994.0 30 13002.5 31 15154.5 32 17313.0 33 19151.0 34 20806.0 35 22183.0 36 24416.5 37 26400.5 38 27273.5 39 28565.5 40 30101.5 41 31405.5 42 32273.5 43 32949.5 44 33778.5 45 34683.5 46 35153.0 47 34942.5 48 34655.5 49 34109.5 50 32798.5 51 31629.0 52 30386.0 53 29024.5 54 27690.0 55 25357.0 56 22582.0 57 20155.5 58 18025.0 59 15793.0 60 13146.0 61 10741.5 62 8720.0 63 6701.5 64 4885.5 65 3572.5 66 2745.0 67 2250.0 68 1747.0 69 1278.0 70 1014.5 71 742.5 72 514.0 73 393.0 74 300.0 75 221.5 76 156.5 77 109.0 78 84.0 79 72.0 80 57.0 81 43.5 82 36.5 83 25.0 84 15.0 85 12.5 86 13.0 87 9.5 88 6.5 89 4.5 90 5.0 91 4.5 92 1.0 93 1.5 94 2.5 95 2.0 96 2.5 97 2.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.141214531007506 2 0.14487126676855336 3 0.13949371417877782 4 0.14358065414700724 5 0.131750038449501 6 0.11271350228169556 7 0.11927411644122174 8 0.10787370495089757 9 0.10550758181139633 10-11 0.09303165980311705 12-13 0.11104646097886514 14-15 0.12954524188769304 16-17 0.14019279601544865 18-19 0.13755779524645861 20-21 0.12830840479204467 22-23 0.12185534168431401 24-25 0.10663686785524917 26-27 0.11034737914219432 28-29 0.11577870725786764 30-31 0.10685196995884022 32-33 0.10432452024164571 34-35 0.11959676959660828 36-37 0.12250064799508707 38-39 0.11023982809039881 40-41 0.11260595122990005 42-43 0.1072283986401245 44-45 0.12319972983175788 46-47 0.12099493326994991 48-49 0.13373973290771798 50-51 0.12185534168431401 52-53 0.10712084758832899 54-55 0.0985167634446881 56-57 0.11809105487147112 58-59 0.1092718686242392 60-61 0.10738972521781777 62-63 0.10787370495089757 64-65 0.12782442505896485 66-67 0.12008074932968807 68-69 0.12449034245330401 70-71 0.112175747022718 72-73 0.11346635964426413 74-75 0.09674217109006218 76-77 0.12190911721021175 78-79 0.12271575009867809 80-81 0.12287707667637136 82-83 0.129115037680511 84-85 0.11916656538942623 86-87 0.12771687400716936 88-89 0.11529472752478782 90-91 0.12330728088355339 92-93 0.13664361130619676 94-95 0.1140041149032417 96-97 0.09916206975546116 98-99 0.10303390762009958 100-101 0.14680718570087256 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 929791.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.20810928048874 #Duplication Level Percentage of deduplicated Percentage of total 1 63.62468632582029 35.762233219385244 2 20.20182745843767 22.71013050898885 3 8.189267292209044 13.809096926628541 4 3.5667312494696373 8.019168793772941 5 1.7620501102076735 4.952075257612508 6 0.9376597661969367 3.1622449563788972 7 0.5292256588006137 2.082274156473248 8 0.32283171107495634 1.4516608076246653 9 0.2136622972674252 1.0808598378534935 >10 0.6409309576360318 5.249205513256372 >50 0.00652384656923828 0.24512976932041683 >100 0.0034524949036701977 0.32235222764767907 >500 3.836104689511588E-4 0.18306154266794383 >1k 7.672209379023176E-4 0.9705064823891817 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3219 0.34620683572975003 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3070 0.33018172901221887 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1411 0.15175453408346606 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1302 0.14003146943775538 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 963 0.10357166287907713 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0755105179551104E-4 2 0.0 0.0 0.0 0.0 1.0755105179551104E-4 3 0.0 0.0 0.0 0.0 1.0755105179551104E-4 4 0.0 0.0 0.0 0.0 1.0755105179551104E-4 5 0.0 2.1510210359102207E-4 0.0 0.0 1.0755105179551104E-4 6 0.0 2.1510210359102207E-4 0.0 0.0 3.226531553865331E-4 7 0.0 2.1510210359102207E-4 0.0 0.0 3.226531553865331E-4 8 0.0 2.1510210359102207E-4 0.0 0.0 3.226531553865331E-4 9 0.0 2.1510210359102207E-4 0.0 0.0 4.3020420718204415E-4 10-11 0.0 2.1510210359102207E-4 0.0 0.0 4.3020420718204415E-4 12-13 0.0 2.1510210359102207E-4 0.0 0.0 4.3020420718204415E-4 14-15 0.0 2.1510210359102207E-4 0.0 0.0 4.3020420718204415E-4 16-17 0.0 2.1510210359102207E-4 0.0 1.0755105179551104E-4 4.3020420718204415E-4 18-19 0.0 2.1510210359102207E-4 0.0 1.0755105179551104E-4 4.3020420718204415E-4 20-21 0.0 2.1510210359102207E-4 0.0 1.0755105179551104E-4 4.3020420718204415E-4 22-23 0.0 2.1510210359102207E-4 0.0 1.0755105179551104E-4 4.3020420718204415E-4 24-25 0.0 2.1510210359102207E-4 0.0 1.6132657769326656E-4 4.3020420718204415E-4 26-27 0.0 2.1510210359102207E-4 0.0 8.066328884663329E-4 4.3020420718204415E-4 28-29 0.0 2.1510210359102207E-4 0.0 0.001667041302830421 4.3020420718204415E-4 30-31 0.0 2.1510210359102207E-4 0.0 0.002903878398478798 4.3020420718204415E-4 32-33 0.0 2.1510210359102207E-4 0.0 0.004732246279002485 6.453063107730662E-4 34-35 0.0 2.1510210359102207E-4 0.0 0.007636124677481283 6.990818366708218E-4 36-37 0.0 3.226531553865331E-4 0.0 0.010271125446471303 7.528573625685773E-4 38-39 0.0 4.3020420718204415E-4 0.0 0.011830615697506213 7.528573625685773E-4 40-41 0.0 4.3020420718204415E-4 0.0 0.012637248585972547 8.604084143640883E-4 42-43 0.0 4.3020420718204415E-4 0.0 0.015379800406758078 8.604084143640883E-4 44-45 0.0 4.3020420718204415E-4 0.0 0.01952051590088525 9.679594661595993E-4 46-47 0.0 4.3020420718204415E-4 0.0 0.023015925084239358 9.679594661595993E-4 48-49 0.0 4.3020420718204415E-4 0.0 0.030544498709925136 9.679594661595993E-4 50-51 0.0 4.3020420718204415E-4 0.0 0.03656735761047375 9.679594661595993E-4 52-53 0.0 4.3020420718204415E-4 0.0 0.04517144175411464 9.679594661595993E-4 54-55 0.0 4.3020420718204415E-4 0.0 0.06474573318089764 9.679594661595993E-4 56-57 0.0 4.3020420718204415E-4 0.0 0.08362094277100983 9.679594661595993E-4 58-59 0.0 4.3020420718204415E-4 0.0 0.0984092123928926 9.679594661595993E-4 60-61 0.0 4.3020420718204415E-4 0.0 0.11739197303480028 9.679594661595993E-4 62-63 0.0 4.3020420718204415E-4 0.0 0.14540902202753092 9.679594661595993E-4 64-65 0.0 4.3020420718204415E-4 0.0 0.17579219415976277 9.679594661595993E-4 66-67 0.0 4.3020420718204415E-4 0.0 0.22128628906926395 9.679594661595993E-4 68-69 0.0 4.3020420718204415E-4 0.0 0.27785814231370276 9.679594661595993E-4 70-71 0.0 4.3020420718204415E-4 0.0 0.33464509766173256 0.001129286043852866 72-73 0.0 4.3020420718204415E-4 0.0 0.3961642992887649 0.0011830615697506215 74-75 0.0 4.3020420718204415E-4 0.0 0.4675244221550865 0.0011830615697506215 76-77 0.0 4.3020420718204415E-4 0.0 0.5480263844240265 0.0011830615697506215 78-79 0.0 4.3020420718204415E-4 0.0 0.6373475329401984 0.0011830615697506215 80-81 0.0 4.3020420718204415E-4 0.0 0.7280130696038142 0.0011830615697506215 82-83 0.0 4.3020420718204415E-4 0.0 0.8298639156541632 0.0011830615697506215 84-85 0.0 4.3020420718204415E-4 0.0 0.9512890531312952 0.0011830615697506215 86-87 0.0 4.3020420718204415E-4 0.0 1.1071843027088883 0.0011830615697506215 88-89 0.0 4.3020420718204415E-4 0.0 1.2770074134940002 0.0011830615697506215 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2185 0.0 57.406017 1 GTATCAA 3400 0.0 36.752064 2 GTGGTAT 890 0.0 36.301437 1 TGGTATC 890 0.0 36.301437 2 TCAACGC 4050 0.0 31.55577 5 ATCAACG 4040 0.0 31.165167 4 CAACGCA 4095 0.0 30.734993 6 TATCAAC 4145 0.0 30.261074 3 AACGCAG 4340 0.0 28.890522 7 GTTGCGA 35 0.008393409 27.142504 34-35 ACGCAGA 4715 0.0 26.19266 8 CGCAGAG 4900 0.0 25.300692 9 GAGTACT 3240 0.0 23.60881 12-13 CGTCAGA 265 3.092282E-11 23.30786 2 GCGTCAG 250 3.8198777E-10 22.805843 1 CAGAGTA 5085 0.0 21.767643 10-11 GTACTTT 3515 0.0 21.627163 14-15 TGCACCG 115 0.003718382 20.656355 5 GCAGAGT 5505 0.0 20.190472 9 GTACATG 2820 0.0 19.375532 1 >>END_MODULE