FastQCFastQC Report
Fri 10 Feb 2017
SRR2031706_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031706_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929791
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40640.4370874744969568No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38150.4103072625998746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19280.20735842786174527No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT14610.15713208667324163No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13550.14573167518291746No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9730.10464717339703225No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9430.1014206418431669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21500.059.011121
GTGGTAT8500.039.6917721
TGGTATC8550.038.3378072
GTATCAA37150.036.582071
TCAACGC44600.030.2501644
ATCAACG45050.029.9479983
CAACGCA45200.029.8437985
TATCAAC45250.029.8156322
AACGCAG47000.028.7008446
ACGCAGA52400.025.7431247
CGCAGAG53500.024.9474838
GAGTACT40700.022.8153212-13
CAGAGTA56150.021.95137810-11
GTACTTT42600.021.5747414-15
GCAGAGT60850.021.1535329
AGAGTAC53100.019.67896810-11
GTACATG25900.018.7137781
AGTACTT42200.018.68412-13
ACATGGG24600.018.3456363
CGCACGG650.0075667918.26841474-75
Produced by FastQC (version 0.11.5)