##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031706_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 929791 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.72108140431559 34.0 31.0 34.0 31.0 34.0 2 32.881702447109085 34.0 31.0 34.0 31.0 34.0 3 32.929516418205814 34.0 31.0 34.0 31.0 34.0 4 36.34061740756794 37.0 37.0 37.0 35.0 37.0 5 36.303827419280246 37.0 37.0 37.0 35.0 37.0 6 36.33482040587616 37.0 37.0 37.0 35.0 37.0 7 36.282887229495664 37.0 37.0 37.0 35.0 37.0 8 36.27212674676352 37.0 37.0 37.0 35.0 37.0 9 38.11667460751932 39.0 39.0 39.0 37.0 39.0 10-11 38.080325040788736 39.0 39.0 39.0 36.0 39.0 12-13 38.02750134169938 39.0 39.0 39.0 35.0 39.0 14-15 39.48462987918791 41.0 39.0 41.0 36.0 41.0 16-17 39.4518106757325 41.0 39.0 41.0 36.0 41.0 18-19 39.47737556074429 41.0 39.0 41.0 36.5 41.0 20-21 39.48633778989041 41.0 39.0 41.0 36.0 41.0 22-23 39.392087576670455 41.0 39.0 41.0 36.0 41.0 24-25 39.320641950717956 41.0 39.0 41.0 36.0 41.0 26-27 39.183646647472386 41.0 39.0 41.0 36.0 41.0 28-29 39.06960811623257 40.5 39.0 41.0 35.0 41.0 30-31 38.88016016502633 40.0 38.5 41.0 35.0 41.0 32-33 38.64100265543547 40.0 38.0 41.0 35.0 41.0 34-35 38.476165611411595 40.0 38.0 41.0 34.5 41.0 36-37 38.35530995675372 40.0 38.0 41.0 34.0 41.0 38-39 38.415887548922285 40.0 38.0 41.0 35.0 41.0 40-41 38.322242848123935 40.0 38.0 41.0 34.0 41.0 42-43 38.196280131771545 40.0 38.0 41.0 34.0 41.0 44-45 37.97966424712651 40.0 38.0 41.0 33.5 41.0 46-47 37.713294170410336 40.0 38.0 41.0 33.0 41.0 48-49 37.41156507215062 40.0 37.5 41.0 33.0 41.0 50-51 37.216766456117554 40.0 37.0 41.0 32.0 41.0 52-53 37.2675730352305 40.0 37.0 41.0 33.0 41.0 54-55 37.18759269556276 40.0 37.0 41.0 32.5 41.0 56-57 37.00415093284405 40.0 36.5 41.0 32.0 41.0 58-59 36.81423782333879 40.0 36.0 41.0 32.0 41.0 60-61 36.554874159891845 39.0 36.0 41.0 31.0 41.0 62-63 36.29285828750763 39.0 35.0 41.0 31.0 41.0 64-65 35.97425873126326 39.0 35.0 41.0 31.0 41.0 66-67 35.623723503454 38.0 35.0 40.5 30.0 41.0 68-69 35.243951597724646 37.0 35.0 40.0 29.5 41.0 70-71 34.890388270052085 37.0 35.0 39.5 29.0 41.0 72-73 34.49783876161417 36.0 35.0 39.0 29.0 41.0 74-75 34.119294013385804 36.0 34.0 39.0 29.0 40.5 76-77 33.190398164748856 35.0 33.5 37.0 27.0 39.0 78-79 33.38213103805049 35.0 34.0 37.0 28.0 39.0 80-81 33.15530371879272 35.0 34.0 37.0 28.0 39.0 82-83 32.87867219622474 35.0 34.0 36.0 28.0 38.0 84-85 32.58453727773231 35.0 34.0 36.0 27.0 37.0 86-87 32.327500481290954 35.0 34.0 35.5 26.5 37.0 88-89 32.09777519894256 35.0 34.0 35.0 26.0 36.5 90-91 31.887244015052843 35.0 34.0 35.0 26.0 36.0 92-93 31.717632779839768 35.0 33.0 35.0 25.5 36.0 94-95 31.528801633915577 35.0 33.0 35.0 25.0 36.0 96-97 31.35515830977069 35.0 33.0 35.0 24.5 35.5 98-99 31.13023625739548 35.0 33.0 35.0 23.5 35.0 100-101 30.41602736528962 34.5 32.0 35.0 17.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15280143598252494 1101 2 0.17737267565106407 1101 3 0.1322276013184691 1101 4 0.1187371286676111 1101 5 0.16825134375716289 1101 6 0.12122678575293833 1101 7 0.09217712519867405 1101 8 0.17335435065719196 1101 9 0.14047857245715534 1101 10-11 0.12382083126958321 1101 12-13 0.16508831862070394 1101 14-15 0.1600001664341093 1101 16-17 0.17584122153910187 1101 18-19 0.12447135216004312 1101 20-21 0.10695799436062003 1101 22-23 -0.12410241348960938 1101 24-25 0.11096408428575444 1101 26-27 0.19237247015425396 1101 28-29 0.17977530421678978 1101 30-31 0.20265573603074216 1101 32-33 0.14513848145985975 1101 34-35 -0.10075894340204883 1101 36-37 0.08008697461417569 1101 38-39 -0.00803928949346755 1101 40-41 -0.4005501041809296 1101 42-43 -0.1922336213260749 1101 44-45 -0.0031713355895490736 1101 46-47 0.15918412921157454 1101 48-49 -0.030812226978135016 1101 50-51 0.20541698759858917 1101 52-53 0.15385043476835136 1101 54-55 0.0495606202330805 1101 56-57 0.029040229882930646 1101 58-59 0.12095099752103522 1101 60-61 0.19244546160274467 1101 62-63 0.16906274980480163 1101 64-65 0.054150592258785935 1101 66-67 -0.0988704323306635 1101 68-69 -0.30097926337948877 1101 70-71 -0.36449822349703 1101 72-73 -0.3663009486084121 1101 74-75 -0.4755949356866367 1101 76-77 -0.5230811558017479 1101 78-79 -0.29077852514848956 1101 80-81 -0.2836736732647225 1101 82-83 -0.16968478760424688 1101 84-85 0.05581155833566953 1101 86-87 0.01552437454321165 1101 88-89 0.11854630604667449 1101 90-91 -0.13467770181381766 1101 92-93 -0.13703565480094326 1101 94-95 -0.20418522046395182 1101 96-97 -0.20897372457388386 1101 98-99 -0.42716271702398245 1101 100-101 -0.7216091312225821 1102 1 0.19761067273316968 1102 2 0.17580834500785159 1102 3 0.14667638748952072 1102 4 0.10475633262622353 1102 5 0.08641525157292307 1102 6 0.11866429434366665 1102 7 0.16067713501664826 1102 8 0.0364557834269732 1102 9 0.14366614159206392 1102 10-11 0.2465373339760717 1102 12-13 0.1939924362793093 1102 14-15 0.3269728266470864 1102 16-17 0.2949708908042581 1102 18-19 0.2227950966047061 1102 20-21 0.27356411684966986 1102 22-23 0.18956060743576586 1102 24-25 0.12195203435808111 1102 26-27 0.22766808937392824 1102 28-29 0.2078350760470542 1102 30-31 0.06777803526951942 1102 32-33 0.2523458567224921 1102 34-35 0.20195539934999118 1102 36-37 0.2012571462978343 1102 38-39 0.08156282071657017 1102 40-41 0.24089745801594376 1102 42-43 0.32630926031496443 1102 44-45 0.35793213931898293 1102 46-47 0.409761753392587 1102 48-49 0.33923542292598086 1102 50-51 0.22631291052094582 1102 52-53 0.32620989045983606 1102 54-55 0.4208404259680876 1102 56-57 0.49289044686018 1102 58-59 0.39060003765037266 1102 60-61 0.45600244840084514 1102 62-63 0.4081353439100823 1102 64-65 0.2979898131043086 1102 66-67 0.27533185722104037 1102 68-69 0.38480136379273944 1102 70-71 0.3179802915015628 1102 72-73 0.29985027438397793 1102 74-75 0.29785529699935864 1102 76-77 0.3732901207313333 1102 78-79 0.3013762584960702 1102 80-81 0.18057908067719808 1102 82-83 0.2117288043999892 1102 84-85 0.25487590534216764 1102 86-87 0.28447470213630055 1102 88-89 0.3684824892108125 1102 90-91 0.29205566162797325 1102 92-93 0.2896748000330973 1102 94-95 0.2738437883787732 1102 96-97 0.04721978116308634 1102 98-99 0.023812862400159673 1102 100-101 0.12748041525243892 1103 1 0.2965697700878209 1103 2 0.28595882664689043 1103 3 0.2503571365971453 1103 4 0.1886672660769051 1103 5 0.19214357781613955 1103 6 0.1693586369186022 1103 7 0.19060232204906669 1103 8 0.22437141102464864 1103 9 0.26737952969044443 1103 10-11 0.19764455367192824 1103 12-13 0.17807176002876446 1103 14-15 0.10927259193973526 1103 16-17 0.29527344324667837 1103 18-19 0.29610752631474213 1103 20-21 0.1871166326190732 1103 22-23 0.1104293913583092 1103 24-25 -0.052305948136663005 1103 26-27 0.1855888511948578 1103 28-29 0.2134521607861899 1103 30-31 0.18083332596435042 1103 32-33 0.21021008096580118 1103 34-35 0.11609009936466208 1103 36-37 0.35574989726355 1103 38-39 0.18787027511977783 1103 40-41 0.22664998845454676 1103 42-43 0.18151193989054093 1103 44-45 0.28928176769900915 1103 46-47 0.2974811803154793 1103 48-49 0.5240598202659683 1103 50-51 0.34619396768196253 1103 52-53 0.3766918351080193 1103 54-55 0.6118579801219184 1103 56-57 0.40766845282565356 1103 58-59 0.38243876191813797 1103 60-61 0.31904245866929415 1103 62-63 0.21173296749817183 1103 64-65 0.3472936368782058 1103 66-67 0.3453070418085957 1103 68-69 0.2639958408354275 1103 70-71 0.40093619830317806 1103 72-73 0.2857418567026926 1103 74-75 0.43428811202063144 1103 76-77 0.632006075941348 1103 78-79 0.6058219457485308 1103 80-81 0.5568244354275791 1103 82-83 0.5478783203160873 1103 84-85 0.36004191189441315 1103 86-87 0.5275862859219345 1103 88-89 0.6004775628013519 1103 90-91 0.6169144704881724 1103 92-93 0.668386415602015 1103 94-95 0.5163258185973483 1103 96-97 0.34106390858962143 1103 98-99 0.5102407876849888 1103 100-101 0.6166068638368571 1104 1 0.14928488526010142 1104 2 0.13726998217706665 1104 3 0.12459566696075086 1104 4 0.03405323599885435 1104 5 0.06457361779329318 1104 6 0.15334397862810079 1104 7 0.09230135117527993 1104 8 0.06761574759422473 1104 9 0.10074855691298268 1104 10-11 0.05758768140846371 1104 12-13 0.13514985825835168 1104 14-15 0.10331171244231285 1104 16-17 0.0693668184608427 1104 18-19 0.13421194283863258 1104 20-21 0.18758065317874895 1104 22-23 0.060350864802060755 1104 24-25 0.031515201682488225 1104 26-27 0.1828166257998518 1104 28-29 0.26616013718062703 1104 30-31 0.14714424353525857 1104 32-33 0.07789878266007833 1104 34-35 0.024124793824974233 1104 36-37 0.1964421207548952 1104 38-39 0.0015898163343166516 1104 40-41 0.05185701301194712 1104 42-43 -0.009283795879497347 1104 44-45 -0.19836219765412721 1104 46-47 -0.057875282658059746 1104 48-49 0.05369169728862033 1104 50-51 0.08675888296846779 1104 52-53 0.10492451167379357 1104 54-55 -0.015866653420822274 1104 56-57 -0.06882117215054961 1104 58-59 -0.1384838061382112 1104 60-61 0.08380188185603998 1104 62-63 0.009919717926500482 1104 64-65 0.1254085236096003 1104 66-67 0.0092252790063867 1104 68-69 0.11081551530715217 1104 70-71 0.16907464300145136 1104 72-73 0.07643883937925722 1104 74-75 0.13620844325992465 1104 76-77 0.09151730761848142 1104 78-79 0.17779867333276655 1104 80-81 0.16682705297944977 1104 82-83 0.11564316897399607 1104 84-85 0.10906627892277498 1104 86-87 -0.011439032943471261 1104 88-89 0.13328619596335045 1104 90-91 0.15906895364065932 1104 92-93 0.1540672170488122 1104 94-95 0.11148572722098748 1104 96-97 0.02989053147178211 1104 98-99 -0.153427592288363 1104 100-101 -0.04651420983532972 1105 1 -0.009336747448770666 1105 2 0.0349749824049681 1105 3 -0.05401320308802582 1105 4 -0.10336186952099524 1105 5 0.016639573397469576 1105 6 0.015740851776378406 1105 7 0.05914692298803459 1105 8 0.018092068301641007 1105 9 -0.1010097274208519 1105 10-11 0.04115290771778035 1105 12-13 0.07137644105610264 1105 14-15 0.045184632812429015 1105 16-17 0.11081127809892877 1105 18-19 -0.018222078766726213 1105 20-21 -0.08023009480429266 1105 22-23 0.12311580395152077 1105 24-25 0.10738750671006159 1105 26-27 -0.02952782928014841 1105 28-29 -0.13755584805115006 1105 30-31 -0.0086257323068466 1105 32-33 -0.12090528771688014 1105 34-35 -0.16443496277286584 1105 36-37 0.1773892924582512 1105 38-39 0.015360954490120093 1105 40-41 0.03195239110575443 1105 42-43 -0.15120001813210848 1105 44-45 -0.1636922035796431 1105 46-47 0.03350023582500228 1105 48-49 0.12543892824718483 1105 50-51 0.27813611073636935 1105 52-53 0.34152371856562524 1105 54-55 0.32505875338897283 1105 56-57 0.21480046055832247 1105 58-59 0.20892833662125554 1105 60-61 0.19225600465106396 1105 62-63 0.08811380152192783 1105 64-65 0.1885495054228059 1105 66-67 0.38264134172574416 1105 68-69 0.4488636259776442 1105 70-71 0.425005861379681 1105 72-73 0.270371447975819 1105 74-75 0.25262213577097725 1105 76-77 0.18965589643292446 1105 78-79 0.3730046991315774 1105 80-81 0.2940776113458341 1105 82-83 0.30964130015590996 1105 84-85 0.41096016652020495 1105 86-87 0.40938689348890733 1105 88-89 0.27378086299466275 1105 90-91 0.1642252962269204 1105 92-93 0.14744088950426004 1105 94-95 0.14526119556503048 1105 96-97 0.1291145161565872 1105 98-99 0.04647042038313742 1105 100-101 0.0011691394513277942 1106 1 0.1775787617166955 1106 2 0.16160048146762307 1106 3 0.12517662710911281 1106 4 0.17582147758889732 1106 5 0.1377368899396103 1106 6 0.061419603998182026 1106 7 0.16010750041500188 1106 8 0.1362193204991371 1106 9 0.030453775499239555 1106 10-11 0.06537129051165635 1106 12-13 0.07522577410851028 1106 14-15 0.17366794156405518 1106 16-17 0.2622190534815658 1106 18-19 0.1776806100937165 1106 20-21 0.2173618434767164 1106 22-23 0.16134575905613957 1106 24-25 0.1351418733394567 1106 26-27 0.12200015334517644 1106 28-29 0.22954288936761458 1106 30-31 0.10254368156804361 1106 32-33 0.12673783994051036 1106 34-35 0.09864061381670552 1106 36-37 0.05111968787254284 1106 38-39 0.007096368894458749 1106 40-41 0.11099135446010422 1106 42-43 -8.060506760898534E-5 1106 44-45 0.05468923966363093 1106 46-47 0.10579138587065984 1106 48-49 0.06636179571667356 1106 50-51 0.132003947600289 1106 52-53 0.0435326652260315 1106 54-55 -0.04255660504471592 1106 56-57 0.020405089482615324 1106 58-59 -0.12852648436449954 1106 60-61 0.039277431254411965 1106 62-63 0.2135183191532235 1106 64-65 0.280238592766203 1106 66-67 0.08691194038483019 1106 68-69 0.05460412960547956 1106 70-71 -0.09494381365556848 1106 72-73 -0.08748618883083026 1106 74-75 -0.08250350105728188 1106 76-77 -0.19994523162425537 1106 78-79 -0.27768385875969415 1106 80-81 -0.1863380625981037 1106 82-83 -0.18084341866720166 1106 84-85 -0.08437808855686058 1106 86-87 -0.07875918667446768 1106 88-89 -0.10610778998188408 1106 90-91 -0.19062715951250198 1106 92-93 -0.22730518957860113 1106 94-95 -0.01692741365067363 1106 96-97 0.030784316315422444 1106 98-99 0.197204900142399 1106 100-101 0.12900278883516236 1107 1 0.10457682246629219 1107 2 0.1137069612989805 1107 3 0.125307559244078 1107 4 0.07205048662176239 1107 5 -0.040640686331997244 1107 6 0.04687158900209454 1107 7 0.053434180456143565 1107 8 0.08659749614896128 1107 9 -0.03688753111617871 1107 10-11 0.11486509232987174 1107 12-13 0.0900014655392809 1107 14-15 0.15206559409634934 1107 16-17 0.030683757814990997 1107 18-19 0.2189977000833636 1107 20-21 0.12271100311063066 1107 22-23 0.10894671864321026 1107 24-25 0.14124403822956566 1107 26-27 0.062028143726031715 1107 28-29 0.1401613400331101 1107 30-31 0.17498552224109432 1107 32-33 0.12923864371833815 1107 34-35 -0.026848381337352123 1107 36-37 0.023297336593266493 1107 38-39 0.03693434765143877 1107 40-41 -0.029791968859349538 1107 42-43 0.12152334268174059 1107 44-45 0.2731575082607236 1107 46-47 0.17293047507763504 1107 48-49 0.18296995511636283 1107 50-51 0.30437392107925376 1107 52-53 0.22813097270996252 1107 54-55 0.3015905103021339 1107 56-57 0.3354001649795393 1107 58-59 0.3254705100156059 1107 60-61 0.3018894691549008 1107 62-63 0.35638419081936235 1107 64-65 0.3502698273544027 1107 66-67 0.263493564205163 1107 68-69 0.3679014517523598 1107 70-71 0.22708159008821838 1107 72-73 0.06539453635861747 1107 74-75 0.2597370969243329 1107 76-77 0.29628683249175225 1107 78-79 0.2222535847758209 1107 80-81 0.2784499219053558 1107 82-83 0.3461627889708012 1107 84-85 0.3813911595580777 1107 86-87 0.28531725441553135 1107 88-89 0.3686732260223877 1107 90-91 0.3929286372168157 1107 92-93 0.4424817838378061 1107 94-95 0.4269936255431297 1107 96-97 0.6956264194346637 1107 98-99 0.5434374734389351 1107 100-101 0.5771463893815891 1108 1 0.08364722428965621 1108 2 0.13215242456799103 1108 3 0.06883156908646271 1108 4 0.06229949552948 1108 5 0.11336321572606778 1108 6 0.03915332368706714 1108 7 -0.009288148925740813 1108 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64-65 -0.628863398969834 2316 66-67 -0.5762910918268815 2316 68-69 -0.7559532905025037 2316 70-71 -0.7425415579678614 2316 72-73 -0.7536085744810208 2316 74-75 -0.7271874332536612 2316 76-77 -1.1728675589058497 2316 78-79 -0.8161489493510814 2316 80-81 -0.6308634206137143 2316 82-83 -0.6795051892675232 2316 84-85 -0.6760421846655618 2316 86-87 -0.5664597594625107 2316 88-89 -0.551734024080595 2316 90-91 -0.6264466380001643 2316 92-93 -0.47432811403005815 2316 94-95 -0.5515856156392722 2316 96-97 -0.5602152828622415 2316 98-99 -0.5814506641766535 2316 100-101 -0.46255232792973544 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 2.0 5 10.0 6 20.0 7 39.0 8 139.0 9 300.0 10 551.0 11 942.0 12 1428.0 13 2007.0 14 2593.0 15 3321.0 16 4280.0 17 6358.0 18 6986.0 19 4125.0 20 3068.0 21 2912.0 22 3194.0 23 3297.0 24 3844.0 25 4387.0 26 4388.0 27 5405.0 28 7035.0 29 8475.0 30 11044.0 31 13921.0 32 18209.0 33 24228.0 34 34253.0 35 54708.0 36 94114.0 37 195870.0 38 305215.0 39 98990.0 40 130.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.06775478503513 19.64744620634089 14.71175763782289 23.573041370801093 2 16.49284624178982 21.63367896656345 38.493489397079564 23.379985394567164 3 18.372070512062123 25.4879163664132 31.60889252180647 24.53112059971821 4 13.710023546723951 19.410288487706545 36.508609237329246 30.37107872824026 5 13.963890809870176 36.38634919030191 34.870094462088794 14.779665537739126 6 29.563095362291097 36.51982004558014 18.617517270010143 15.299567322118627 7 25.155330606555665 33.27543501711675 23.28189883532966 18.287335540997923 8 24.251148914110807 33.97871134480759 21.77198961917248 19.998150121909116 9 25.08337895290447 16.48230623871386 21.699177557106918 36.73513725127474 10-11 23.604928419397478 26.318280129620526 29.20387484929409 20.87291660168791 12-13 24.7756349414196 25.028326273499246 28.472811886118432 21.72322689896272 14-15 22.230653034205016 26.745057085440216 26.987692388769297 24.03659749158547 16-17 21.162717161820176 29.024208978692783 28.06860555035121 21.74446830913583 18-19 20.849439120661657 27.366663440131646 30.541358801449793 21.242538637756915 20-21 21.88604750960162 26.33876860498757 30.56660045106911 21.208583434341698 22-23 21.752899307478778 26.593664597742933 30.221038921650134 21.432397173128155 24-25 21.432289622076357 26.871253862427146 30.51497594620727 21.181480569289228 26-27 21.154188457039915 26.952932793783525 30.73756100184182 21.15531774733474 28-29 21.048105715121583 26.821845583300348 30.804124576227437 21.325924125350635 30-31 21.434237067382384 26.725966763480592 30.587750681470716 21.252045487666308 32-33 21.386495875412727 26.875692360640574 30.312651243829254 21.425160520117444 34-35 21.362441901364896 26.977558679487 30.460895221847778 21.19910419730033 36-37 21.348292249554046 26.975202380433807 30.412322068434783 21.264183301577365 38-39 21.16819801439248 27.027740642789617 30.565578716077052 21.238482626740847 40-41 21.38243970956914 26.986172161270648 30.35139079642629 21.279997332733917 42-43 21.414167269848818 27.044088402662535 30.11031511382666 21.431429213661996 44-45 21.456327282152657 27.188744567327493 29.935759756762543 21.419168393757307 46-47 21.47057779651556 27.283497043959343 29.691833971290322 21.554091188234775 48-49 21.45267054639161 27.46504321939016 29.573151385633977 21.509134848584253 50-51 21.463479427097056 27.714938088237034 29.261038233323404 21.560544251342506 52-53 21.717461235912154 27.825823222638206 28.926930890920648 21.529784650528992 54-55 21.54010955150136 27.880459156950327 28.92633936013577 21.653091931412543 56-57 21.490152087942345 28.04544247040464 28.832017087711108 21.63238835394191 58-59 21.41251554117713 28.114204528344093 29.111044668246954 21.36223526223182 60-61 21.49032235859974 28.092424809523198 29.166881773165894 21.250371058711167 62-63 21.405637738665956 28.198750348230988 28.946573030626045 21.449038882477016 64-65 21.459481580630015 28.17626108004304 28.872348331869706 21.49190900745723 66-67 21.313712931428082 28.319009536470425 28.8909396270919 21.47633790500959 68-69 21.283684041552593 28.21944122229195 28.99899597907635 21.4978787570791 70-71 21.413479703223466 28.07675492489975 28.941842275222214 21.567923096654567 72-73 21.395320571908588 28.121958118978757 28.788872886615348 21.693848422497307 74-75 21.515554365257348 28.2482503154111 28.547581633124995 21.68861368620656 76-77 21.553286433198497 28.323458205569725 28.44235768361661 21.68089767761517 78-79 21.476919701458346 28.298647054995957 28.474655203103076 21.749778040442617 80-81 21.40986761046489 28.19573881771741 28.53476616846927 21.859627403348433 82-83 21.538112373835837 28.238507074658635 28.455299419215947 21.76808113228958 84-85 21.57704508800828 28.245679982318215 28.326506379800175 21.85076854987333 86-87 21.54030731074329 28.16997221372256 28.311242875296237 21.978477600237905 88-89 21.46721697161986 28.167134963634165 28.342927819528658 22.02272024521732 90-91 21.52386934268024 28.094808403178778 28.42563543850177 21.955686815639215 92-93 21.50988770594682 28.11793187931481 28.33556143262303 22.036618982115336 94-95 21.511178318568366 28.115243103019928 28.270277944183153 22.103300634228553 96-97 21.57936568540672 28.020598177439876 28.237851302066808 22.162184835086595 98-99 21.487636193314515 28.027765394462516 28.160375912638386 22.324222499584582 100-101 21.420289917421503 28.19015640225343 28.097446498211408 22.292107182113657 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1502.0 1 881.5 2 277.0 3 296.0 4 234.0 5 211.0 6 370.0 7 754.5 8 1199.5 9 1433.0 10 1250.0 11 958.0 12 900.5 13 958.0 14 1079.0 15 1161.5 16 1232.0 17 1358.0 18 1486.5 19 1632.0 20 1815.0 21 2054.0 22 2564.5 23 3217.0 24 3860.5 25 4767.0 26 6062.0 27 7714.0 28 9477.0 29 11364.0 30 13463.5 31 15708.0 32 17874.0 33 19837.5 34 21442.0 35 22811.0 36 25088.0 37 27004.0 38 28229.0 39 30067.0 40 31836.0 41 32976.0 42 33997.5 43 35067.5 44 35801.5 45 36357.5 46 36616.0 47 36627.0 48 36129.5 49 34878.5 50 32841.0 51 30985.5 52 29478.0 53 27720.0 54 25472.0 55 22678.0 56 20222.5 57 17830.0 58 15816.0 59 13727.0 60 11107.0 61 9089.5 62 7487.5 63 5779.0 64 4103.5 65 2933.5 66 2286.0 67 1854.0 68 1490.5 69 1098.0 70 778.0 71 563.5 72 395.5 73 290.5 74 229.5 75 156.5 76 97.5 77 65.5 78 34.5 79 17.0 80 13.0 81 9.5 82 5.5 83 3.5 84 1.5 85 0.5 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01871388301241892 2 0.0 3 0.002258572087705732 4 0.016132657769326656 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.377552589775552E-5 14-15 5.377552589775552E-5 16-17 0.0012906126215461323 18-19 1.0755105179551104E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.764286812842886E-4 28-29 0.0050548994343890185 30-31 5.377552589775552E-5 32-33 1.0755105179551104E-4 34-35 0.013121228319052346 36-37 0.004786021804900241 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.2265315538653307E-4 60-61 0.0020434699841147094 62-63 0.009894696765187016 64-65 0.0024198986653989982 66-67 0.004786021804900241 68-69 0.0031727560279675754 70-71 5.377552589775552E-5 72-73 0.006668165211321684 74-75 0.005592654693366573 76-77 0.0015057147251371543 78-79 4.8397973307979966E-4 80-81 0.007797451255174549 82-83 0.010701329653653347 84-85 0.002204796561807976 86-87 0.001667041302830421 88-89 3.764286812842886E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 5.377552589775552E-5 100-101 9.679594661595993E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 929791.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.74924614097025 #Duplication Level Percentage of deduplicated Percentage of total 1 61.10901960232498 32.845637360387414 2 21.336636512010713 22.936562554089537 3 8.772444530629757 14.145368410044812 4 3.9370922053666497 8.4646295210379 5 1.8752394186248573 5.039635254245871 6 1.0230275380160632 3.2992175369889716 7 0.5911154676055845 2.22404075362471 8 0.3784575701426121 1.627344727320702 9 0.2346221358793846 1.134968663835109 >10 0.7279111684761683 5.743137668118533 >50 0.006817235427779068 0.24301028528592047 >100 0.006013117711745961 0.6555583824366433 >500 6.013116688906022E-4 0.28096895842549807 >1k 0.0010021861148176703 1.3599199241583828 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4064 0.4370874744969568 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3815 0.4103072625998746 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1928 0.20735842786174527 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 1461 0.15713208667324163 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1355 0.14573167518291746 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 973 0.10464717339703225 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 943 0.1014206418431669 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.0755105179551104E-4 0.0 18-19 0.0 0.0 0.0 1.0755105179551104E-4 0.0 20-21 0.0 0.0 0.0 1.0755105179551104E-4 0.0 22-23 0.0 0.0 0.0 1.0755105179551104E-4 0.0 24-25 0.0 0.0 0.0 1.6132657769326656E-4 0.0 26-27 0.0 0.0 0.0 8.066328884663329E-4 0.0 28-29 0.0 0.0 0.0 0.00155949025103491 0.0 30-31 0.0 0.0 0.0 0.002903878398478798 0.0 32-33 0.0 0.0 0.0 0.004893572856695752 0.0 34-35 0.0 0.0 0.0 0.007689900203379039 0.0 36-37 0.0 0.0 0.0 0.010432452024164571 0.0 38-39 0.0 0.0 0.0 0.012260819904688258 0.0 40-41 0.0 0.0 0.0 0.01295990174135908 0.0 42-43 0.0 0.0 0.0 0.015917555665735633 0.0 44-45 0.0 0.0 0.0 0.02016582221165832 5.377552589775552E-5 46-47 0.0 0.0 0.0 0.023607455869114672 1.0755105179551104E-4 48-49 0.0 0.0 0.0 0.031028478443004937 1.0755105179551104E-4 50-51 0.0 0.0 0.0 0.03737399049894008 1.0755105179551104E-4 52-53 0.0 0.0 0.0 0.0463007277979675 1.0755105179551104E-4 54-55 0.0 0.0 0.0 0.065767468172955 1.0755105179551104E-4 56-57 0.0 0.0 0.0 0.08496533091845372 1.0755105179551104E-4 58-59 0.0 0.0 0.0 0.09953849843674546 1.0755105179551104E-4 60-61 0.0 0.0 0.0 0.11852125907865316 1.0755105179551104E-4 62-63 0.0 0.0 0.0 0.14648453254548602 1.0755105179551104E-4 64-65 0.0 0.0 0.0 0.1768677046777179 1.0755105179551104E-4 66-67 0.0 0.0 0.0 0.22177026880234374 1.0755105179551104E-4 68-69 0.0 0.0 0.0 0.2775354891583162 1.0755105179551104E-4 70-71 0.0 0.0 0.0 0.33491397529122136 1.0755105179551104E-4 72-73 0.0 0.0 0.0 0.39659450349594694 1.0755105179551104E-4 74-75 0.0 0.0 0.0 0.4682235039917573 1.0755105179551104E-4 76-77 0.0 0.0 0.0 0.5489943438901861 1.0755105179551104E-4 78-79 0.0 1.0755105179551104E-4 0.0 0.6388532476653355 1.0755105179551104E-4 80-81 0.0 1.0755105179551104E-4 0.0 0.7300565395879288 2.1510210359102207E-4 82-83 0.0 1.0755105179551104E-4 0.0 0.8328753451044375 2.688776294887776E-4 84-85 0.0 1.0755105179551104E-4 0.0 0.9565590546692752 3.226531553865331E-4 86-87 0.0 1.0755105179551104E-4 0.0 1.1146591008086764 3.226531553865331E-4 88-89 0.0 1.0755105179551104E-4 0.0 1.2871172123627783 3.226531553865331E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2150 0.0 59.01112 1 GTGGTAT 850 0.0 39.691772 1 TGGTATC 855 0.0 38.337807 2 GTATCAA 3715 0.0 36.58207 1 TCAACGC 4460 0.0 30.250164 4 ATCAACG 4505 0.0 29.947998 3 CAACGCA 4520 0.0 29.843798 5 TATCAAC 4525 0.0 29.815632 2 AACGCAG 4700 0.0 28.700844 6 ACGCAGA 5240 0.0 25.743124 7 CGCAGAG 5350 0.0 24.947483 8 GAGTACT 4070 0.0 22.81532 12-13 CAGAGTA 5615 0.0 21.951378 10-11 GTACTTT 4260 0.0 21.57474 14-15 GCAGAGT 6085 0.0 21.153532 9 AGAGTAC 5310 0.0 19.678968 10-11 GTACATG 2590 0.0 18.713778 1 AGTACTT 4220 0.0 18.684 12-13 ACATGGG 2460 0.0 18.345636 3 CGCACGG 65 0.00756679 18.268414 74-75 >>END_MODULE