FastQCFastQC Report
Fri 10 Feb 2017
SRR2031703_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031703_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931982
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46680.24161715792383157No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45800.2370622500623712No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20530.106263929995207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36900.056.3911931
GTATCAA61550.036.045611
GTGGTAT17000.034.373181
TGGTATC17250.032.2225762
CGGTCCG300.003951489531.66766244-45
ATCAACG69900.031.53423
TCAACGC70300.031.3539624
CAACGCA72000.030.5476825
TATCAAC72650.030.4729022
AACGCAG74950.029.530126
ACGCAGA82550.026.6401947
CGCAGAG83300.026.344688
GCAGAGT92050.022.9115879
GAGTACT61700.022.90536512-13
CGGAGAT2755.2750693E-1122.4581621
CAGAGTA90250.022.02786410-11
GTACTTT66550.021.09358814-15
TACATGG47750.019.0030572
AGAGTAC83350.018.60808410-11
GTACATG51150.018.4829371
Produced by FastQC (version 0.11.5)