##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031703_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1931982 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.774057936357586 33.0 31.0 34.0 30.0 34.0 2 32.1052188892029 33.0 31.0 34.0 30.0 34.0 3 32.20918259072807 34.0 31.0 34.0 30.0 34.0 4 35.67260046936255 37.0 35.0 37.0 35.0 37.0 5 35.67713312028787 37.0 35.0 37.0 35.0 37.0 6 35.70219701839872 37.0 36.0 37.0 35.0 37.0 7 35.6808386413538 37.0 36.0 37.0 35.0 37.0 8 35.6727309053604 37.0 36.0 37.0 35.0 37.0 9 37.420354848026534 39.0 38.0 39.0 35.0 39.0 10-11 37.34299569043604 39.0 38.0 39.0 35.0 39.0 12-13 37.295145865748225 39.0 38.0 39.0 34.5 39.0 14-15 38.63960197351736 41.0 38.0 41.0 34.5 41.0 16-17 38.56203965668417 41.0 38.0 41.0 34.0 41.0 18-19 38.5867606426975 40.5 38.5 41.0 34.0 41.0 20-21 38.6017768281485 41.0 39.0 41.0 34.0 41.0 22-23 38.49684158548061 40.0 38.5 41.0 34.0 41.0 24-25 38.42487015924579 40.0 38.0 41.0 34.0 41.0 26-27 38.30746352709291 40.0 38.0 41.0 34.0 41.0 28-29 38.19115680166792 40.0 38.0 41.0 34.0 41.0 30-31 38.023200526712984 40.0 38.0 41.0 33.5 41.0 32-33 37.800278936346196 40.0 38.0 41.0 33.0 41.0 34-35 37.625679742357846 40.0 38.0 41.0 33.0 41.0 36-37 37.446528746127036 40.0 37.5 41.0 32.0 41.0 38-39 37.24874869434601 40.0 37.0 41.0 31.5 41.0 40-41 37.155563302349606 40.0 37.0 41.0 31.0 41.0 42-43 37.12557259850247 40.0 37.0 41.0 31.0 41.0 44-45 36.98638315470848 40.0 37.0 41.0 31.0 41.0 46-47 36.89106549646943 40.0 37.0 41.0 30.5 41.0 48-49 36.91894800262114 40.0 37.5 41.0 31.0 41.0 50-51 36.38326495795509 39.5 36.5 40.5 30.5 41.0 52-53 36.41752692312868 39.5 37.0 40.5 30.0 41.0 54-55 36.67339343741298 40.0 37.0 41.0 30.0 41.0 56-57 36.57565908999152 40.0 37.0 41.0 30.0 41.0 58-59 36.414348580887406 40.0 36.5 41.0 29.5 41.0 60-61 36.20418979058811 40.0 36.0 41.0 29.0 41.0 62-63 35.944105328103475 39.0 35.5 41.0 28.0 41.0 64-65 35.69678780651165 39.0 35.0 41.0 28.0 41.0 66-67 35.42956947839059 39.0 35.0 41.0 28.0 41.0 68-69 35.103035380246816 38.0 35.0 40.5 28.0 41.0 70-71 34.73905502225176 37.0 35.0 40.0 27.5 41.0 72-73 34.365909206193436 37.0 34.5 39.0 26.0 41.0 74-75 33.943195640539095 36.0 34.0 39.0 26.0 41.0 76-77 33.54370045890697 36.0 34.0 39.0 26.0 40.0 78-79 33.13021756931482 35.5 34.0 37.0 26.0 39.0 80-81 32.75518974814465 35.0 34.0 37.0 25.5 39.0 82-83 32.4026354800407 35.0 34.0 36.5 25.0 39.0 84-85 32.055053307950075 35.0 33.5 36.0 24.5 37.0 86-87 31.754868834181686 35.0 33.0 36.0 23.5 37.0 88-89 31.466412471751806 35.0 33.0 35.5 22.0 37.0 90-91 31.214917116205015 35.0 33.0 35.0 20.0 36.0 92-93 30.98945098867381 35.0 33.0 35.0 19.5 36.0 94-95 30.732183063817367 35.0 32.0 35.0 17.5 36.0 96-97 30.458247281806976 35.0 32.0 35.0 6.0 35.5 98-99 30.20553322960566 35.0 32.0 35.0 2.0 35.0 100-101 29.613577403930265 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.12500475043677994 1101 2 -0.08762275988351576 1101 3 0.14446107067502822 1101 4 0.15022827940040884 1101 5 -0.012615025274200775 1101 6 0.15155369952407227 1101 7 0.21534794825630144 1101 8 0.15580701356032733 1101 9 0.2663867392262702 1101 10-11 0.06520007467070599 1101 12-13 0.10717445812353077 1101 14-15 0.07505403242444686 1101 16-17 0.18535913118056868 1101 18-19 0.2259956921095707 1101 20-21 0.20916890323928783 1101 22-23 0.21195216826222918 1101 24-25 0.15271666090935554 1101 26-27 0.11822623088961137 1101 28-29 0.02607122633247627 1101 30-31 0.12307404365051156 1101 32-33 -0.08660155825403848 1101 34-35 -0.017716977344292673 1101 36-37 -0.08866048763329104 1101 38-39 0.14979719118581158 1101 40-41 -0.21428639873634125 1101 42-43 -0.0361735936708385 1101 44-45 -0.12525748408342707 1101 46-47 -0.24192183426755776 1101 48-49 0.08829540406551217 1101 50-51 0.13948234815062932 1101 52-53 0.06470348935497583 1101 54-55 0.2586232899422143 1101 56-57 0.003479272861738991 1101 58-59 -0.14040579866776426 1101 60-61 -0.4450702689081183 1101 62-63 -0.23175769071936259 1101 64-65 0.1848424540752447 1101 66-67 -0.10646297953175576 1101 68-69 -0.41817004489275433 1101 70-71 -0.4746050288871615 1101 72-73 -0.005571897776128765 1101 74-75 0.019959944688928033 1101 76-77 -0.24376410707490948 1101 78-79 -0.45898918898318186 1101 80-81 -0.5922669349678742 1101 82-83 -0.5580616778268492 1101 84-85 -0.4005874873142581 1101 86-87 -0.3469880038844799 1101 88-89 -0.5308808055587448 1101 90-91 -0.8580576671510975 1101 92-93 -0.6919529876396808 1101 94-95 -0.6426937841308877 1101 96-97 -0.6448154992121147 1101 98-99 -0.6723048928643216 1101 100-101 -0.5736728282773917 1102 1 0.1695784798377673 1102 2 0.19245045307715714 1102 3 0.2934336158147772 1102 4 0.35637816809691714 1102 5 0.29858358961573117 1102 6 0.3831737836200446 1102 7 0.29663214316106945 1102 8 0.17440904175721528 1102 9 0.32898777805881707 1102 10-11 0.3888082863946707 1102 12-13 0.38593676334243554 1102 14-15 0.548594472691569 1102 16-17 0.4182822081036477 1102 18-19 0.5066100873606203 1102 20-21 0.4764110501598964 1102 22-23 0.40779288060455343 1102 24-25 0.31182227555194686 1102 26-27 0.31233753437712153 1102 28-29 0.4637501776062791 1102 30-31 0.28097263380633564 1102 32-33 0.4490699727872709 1102 34-35 0.4573668712829715 1102 36-37 0.5643842811027966 1102 38-39 0.3428874706812408 1102 40-41 0.447190722213449 1102 42-43 0.6154168094951231 1102 44-45 0.2143428127258744 1102 46-47 0.340874109338678 1102 48-49 0.21405647257899574 1102 50-51 0.2022832385785307 1102 52-53 0.1617789283853952 1102 54-55 0.270236448487843 1102 56-57 0.3430271333613675 1102 58-59 0.2031806470408597 1102 60-61 0.10590870709979328 1102 62-63 0.16050646955766013 1102 64-65 0.14489785847791126 1102 66-67 0.13959193018875737 1102 68-69 0.2869301282460057 1102 70-71 0.13677859713312301 1102 72-73 0.2766086612147163 1102 74-75 0.0065758239864877055 1102 76-77 0.34503430994215734 1102 78-79 0.15725419464283874 1102 80-81 0.1766858228852115 1102 82-83 0.28557918292012374 1102 84-85 0.3408164206748623 1102 86-87 0.24889821921717825 1102 88-89 0.30562179152262914 1102 90-91 0.23677686166413991 1102 92-93 0.39397924090348013 1102 94-95 6.354268510584404E-4 1102 96-97 -0.37834505647157357 1102 98-99 -0.17028856835282014 1102 100-101 -0.2020735208324247 1103 1 -0.037060714301425435 1103 2 0.23550539813210492 1103 3 0.21108563046679052 1103 4 0.17692212414087294 1103 5 0.3533052013373492 1103 6 0.2320199374661982 1103 7 0.29873104425997354 1103 8 0.39775885860703397 1103 9 0.2987918073628464 1103 10-11 0.24092641826279504 1103 12-13 0.3117755911812594 1103 14-15 0.5051594910065944 1103 16-17 0.5279186549900956 1103 18-19 0.43162748662801675 1103 20-21 0.2192544567532977 1103 22-23 0.1639091809708475 1103 24-25 0.3697517627314326 1103 26-27 0.3163457761353641 1103 28-29 0.13647545233155256 1103 30-31 0.23381512464882803 1103 32-33 0.07184469806200156 1103 34-35 0.23095569912179315 1103 36-37 0.11416450088301389 1103 38-39 0.03463106042482167 1103 40-41 0.22119438521711743 1103 42-43 0.2132464798247966 1103 44-45 0.14492797756103926 1103 46-47 0.25006733278189586 1103 48-49 0.4672725897951153 1103 50-51 0.3589673045125963 1103 52-53 0.5069309430374105 1103 54-55 0.4746430418944385 1103 56-57 0.4513165106507486 1103 58-59 0.3907676433778562 1103 60-61 0.45979881698990255 1103 62-63 0.5219936490448447 1103 64-65 0.6212476753277301 1103 66-67 0.4439188532656715 1103 68-69 0.3612387363046139 1103 70-71 0.2840029561074857 1103 72-73 0.1893934597495175 1103 74-75 0.29854285695351024 1103 76-77 0.3456039070117498 1103 78-79 0.2549941213827651 1103 80-81 0.325088753288135 1103 82-83 0.21901782760876642 1103 84-85 0.14926513862357638 1103 86-87 0.30453741335636764 1103 88-89 0.21411996002079903 1103 90-91 0.19613675177402712 1103 92-93 -0.31346672979249135 1103 94-95 0.04411528033091017 1103 96-97 0.10568882631230991 1103 98-99 -0.04424735956161285 1103 100-101 -0.16392700068590216 1104 1 0.09936448330797987 1104 2 0.16618787355142217 1104 3 0.31570776272049983 1104 4 0.22648111777881752 1104 5 0.16895490251336298 1104 6 0.26396036545925483 1104 7 0.2215043236122014 1104 8 0.1644472141111777 1104 9 0.28000686423580134 1104 10-11 0.35807144428940063 1104 12-13 0.41641218265995406 1104 14-15 0.5095707595625996 1104 16-17 0.5542428790116887 1104 18-19 0.4504666518487639 1104 20-21 0.48810567132819926 1104 22-23 0.5935927649307473 1104 24-25 0.4913526399244148 1104 26-27 0.4858042781596552 1104 28-29 0.5123863835056497 1104 30-31 0.5552051381441103 1104 32-33 0.39021514340613095 1104 34-35 0.41948832877284303 1104 36-37 0.5233316495238469 1104 38-39 0.3747023926014208 1104 40-41 0.5435146088531297 1104 42-43 0.5462207424043228 1104 44-45 0.6004550163117628 1104 46-47 0.6395253528320239 1104 48-49 0.7075463511215077 1104 50-51 0.5013509077514655 1104 52-53 0.5772051863379701 1104 54-55 0.6988333252952472 1104 56-57 0.5844522345759415 1104 58-59 0.6164484318875978 1104 60-61 0.5607820442889206 1104 62-63 0.6612745435888954 1104 64-65 0.4966549434981573 1104 66-67 0.5589094547694344 1104 68-69 0.663182297129751 1104 70-71 0.6780903380238144 1104 72-73 0.49021884050910813 1104 74-75 0.5677302485093279 1104 76-77 0.5953315916078594 1104 78-79 0.41849421777759943 1104 80-81 0.3298217396000709 1104 82-83 0.3613339544643708 1104 84-85 0.4973878492462873 1104 86-87 0.48163159110844234 1104 88-89 0.5934529077748003 1104 90-91 0.5141927089747256 1104 92-93 0.32274037450671855 1104 94-95 0.5223589664693336 1104 96-97 0.5377220343323614 1104 98-99 0.5279439359849469 1104 100-101 0.5559785323775266 1105 1 -0.08639949138269643 1105 2 0.100434464446856 1105 3 0.12017229053497402 1105 4 0.05280879520653059 1105 5 0.17321887119327783 1105 6 0.1790753647179315 1105 7 0.17427541905377097 1105 8 0.21315944147297472 1105 9 0.16786004666781196 1105 10-11 0.20519769836837298 1105 12-13 -0.07046899256447858 1105 14-15 0.10592312787011338 1105 16-17 0.147772526099736 1105 18-19 0.06896183722738414 1105 20-21 0.1298965715668885 1105 22-23 -0.041456897331137554 1105 24-25 0.17153678967522978 1105 26-27 0.10907301137125813 1105 28-29 -0.10554863212902177 1105 30-31 0.1823002230761901 1105 32-33 0.2500985746703819 1105 34-35 0.09514587288182952 1105 36-37 0.26033738112055715 1105 38-39 0.03811450923159754 1105 40-41 0.045688501570353424 1105 42-43 0.16191441120058414 1105 44-45 0.14848084790086347 1105 46-47 0.23413543461848008 1105 48-49 0.19862637842373232 1105 50-51 0.04164422632057807 1105 52-53 0.1946838848607939 1105 54-55 0.12253152754274055 1105 56-57 0.18664038615937528 1105 58-59 0.12013570122455519 1105 60-61 0.1388321393257641 1105 62-63 0.22197440347132158 1105 64-65 0.20529721893270647 1105 66-67 0.29967809114096866 1105 68-69 0.3824026810973109 1105 70-71 0.25242578135767957 1105 72-73 0.09871862746094706 1105 74-75 0.18462005919699465 1105 76-77 0.3511480068687831 1105 78-79 0.5272282575314549 1105 80-81 0.5735985960243042 1105 82-83 0.5883603163363631 1105 84-85 0.559090003904565 1105 86-87 0.5164627955184748 1105 88-89 0.42476551154394926 1105 90-91 0.3844677481448642 1105 92-93 0.5577522023531181 1105 94-95 0.6085027212195051 1105 96-97 0.5097132132033231 1105 98-99 0.5757398558788367 1105 100-101 0.6116806095637379 1106 1 0.024049954910577043 1106 2 0.1557408419755646 1106 3 0.16635395160371047 1106 4 0.05357261730027574 1106 5 0.21661785379253473 1106 6 0.1746145922546134 1106 7 0.1766581839354444 1106 8 0.18351954372740664 1106 9 0.21486853872354317 1106 10-11 0.2312004398981813 1106 12-13 0.21686892027236127 1106 14-15 0.1875800817373161 1106 16-17 0.2728869708242243 1106 18-19 0.18267810175156995 1106 20-21 0.236524978547763 1106 22-23 0.2104815905582953 1106 24-25 0.23899359643596085 1106 26-27 0.15323131542903212 1106 28-29 0.3116345360095707 1106 30-31 0.2927927468781206 1106 32-33 0.5309562157202876 1106 34-35 0.34163087676523674 1106 36-37 0.41412472996351113 1106 38-39 0.48244100845749216 1106 40-41 0.4739650926092054 1106 42-43 0.4870255125960412 1106 44-45 0.6062288843380017 1106 46-47 0.5738112345742437 1106 48-49 0.5905995597813316 1106 50-51 0.46734902579799353 1106 52-53 0.6592761015908124 1106 54-55 0.5931603820153484 1106 56-57 0.6293337977437616 1106 58-59 0.5487768437172349 1106 60-61 0.5500628958954081 1106 62-63 0.7761324760678576 1106 64-65 0.8290016789336292 1106 66-67 0.8045953566064341 1106 68-69 0.88835123497892 1106 70-71 0.8432132382158741 1106 72-73 0.6324328859877184 1106 74-75 0.7943191853022284 1106 76-77 0.7776738508021808 1106 78-79 0.8634520702638753 1106 80-81 0.9187863882441221 1106 82-83 0.8779536903725784 1106 84-85 0.834556526893806 1106 86-87 0.7692062541666367 1106 88-89 0.8753459648993633 1106 90-91 0.9772952786591702 1106 92-93 1.012089400231904 1106 94-95 1.1112538952423527 1106 96-97 1.1372477137871186 1106 98-99 1.12453445717399 1106 100-101 1.2259658829709004 1107 1 0.19153811016559885 1107 2 0.2711001381201541 1107 3 0.3462669414849202 1107 4 0.272670779251456 1107 5 0.23160267095281029 1107 6 0.2229494054877179 1107 7 0.2838326581272739 1107 8 0.3097557703424343 1107 9 0.29721031702612777 1107 10-11 0.2911318874797715 1107 12-13 0.2690878047696188 1107 14-15 0.34431694269019175 1107 16-17 0.45469565239994125 1107 18-19 0.33777786518948716 1107 20-21 0.3011993162870681 1107 22-23 0.4510816754916718 1107 24-25 0.4049965824166293 1107 26-27 0.44912745575281576 1107 28-29 0.5185137069639509 1107 30-31 0.5411173760933394 1107 32-33 0.5451635862867406 1107 34-35 0.5338783430747753 1107 36-37 0.6202314464897114 1107 38-39 0.6552028874820124 1107 40-41 0.6760409682017752 1107 42-43 0.6917954935725632 1107 44-45 0.8114290235861432 1107 46-47 0.7704436527659624 1107 48-49 0.6375455525367002 1107 50-51 0.7213888526297509 1107 52-53 0.7027294088672633 1107 54-55 0.8426197304778285 1107 56-57 0.8591218503598483 1107 58-59 0.9019364519054491 1107 60-61 0.7683463364160801 1107 62-63 0.8291564304754075 1107 64-65 0.7478739953117923 1107 66-67 0.8077375920582668 1107 68-69 0.8790373515666161 1107 70-71 0.7566033247068091 1107 72-73 0.761067348970478 1107 74-75 0.7359158396193877 1107 76-77 0.7653563477369971 1107 78-79 0.6236482357481776 1107 80-81 0.9058518535073077 1107 82-83 0.8991421053533344 1107 84-85 0.8897737060672952 1107 86-87 0.9239810276221583 1107 88-89 0.9402008526288874 1107 90-91 1.0137510673747414 1107 92-93 1.102125270605999 1107 94-95 1.1397618234669942 1107 96-97 1.189372079948491 1107 98-99 1.15549486996618 1107 100-101 1.185256103518121 1108 1 -0.06806162860718246 1108 2 0.041656296155700545 1108 3 0.13180467558088793 1108 4 0.11887466791483092 1108 5 0.2305458766711297 1108 6 0.23492630253721103 1108 7 0.26224085112853857 1108 8 0.3081553291363264 1108 9 0.24353736734381215 1108 10-11 0.139467129918323 1108 12-13 0.2677448805277223 1108 14-15 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-0.3050535253405542 2316 94-95 -0.4202266269687449 2316 96-97 -0.522946385861637 2316 98-99 -0.5008956955233295 2316 100-101 -0.6388640101299536 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9639.0 3 1513.0 4 1167.0 5 1226.0 6 1432.0 7 1770.0 8 2170.0 9 2766.0 10 3424.0 11 4027.0 12 5029.0 13 6367.0 14 7529.0 15 8716.0 16 9764.0 17 12798.0 18 11958.0 19 7657.0 20 6996.0 21 7018.0 22 7326.0 23 8320.0 24 9230.0 25 10949.0 26 12776.0 27 15101.0 28 17684.0 29 21229.0 30 25922.0 31 32364.0 32 40947.0 33 53998.0 34 73994.0 35 111425.0 36 186776.0 37 380306.0 38 606282.0 39 204194.0 40 193.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.5198791627229 20.424568449403537 14.58314975176316 23.472402636110402 2 15.87347993926755 22.097467390521157 39.511393036295885 22.517659633915414 3 18.218959245387076 26.079873360376602 31.51285419130918 24.18831320292714 4 13.126408635414572 19.60162183568909 38.30767811268254 28.964291416213793 5 13.539039002462436 37.257248530754005 35.351984123487675 13.85172834329589 6 28.916569807059812 37.60491540697358 19.317648899946054 14.160865886020554 7 24.71187556195861 33.604852731216006 23.939605158488522 17.74366654833686 8 23.566467084485186 34.81880856951134 22.360216906363917 19.25450743963956 9 24.979079172701105 17.08730267708865 22.457491210661722 35.47612693954852 10-11 23.045379103857442 27.186895529649114 29.962430390790768 19.805294975702672 12-13 24.232166360932332 25.83652022510338 28.954932686627494 20.976380727336792 14-15 21.83960381671081 27.662782923277064 27.38526285237456 23.112350407637567 16-17 20.797013273619537 29.680554529656682 28.44996831592329 21.072463880800484 18-19 20.562038165849774 28.56718935303826 30.40897979747717 20.461792683634794 20-21 21.738052170848384 27.81145208688576 30.129805175621815 20.320690566644036 22-23 21.36058811030437 28.05034282249413 29.60242437434247 20.98664469285903 24-25 21.04372725237736 28.124455922465323 30.098602394355687 20.733214430801628 26-27 20.915173065642442 28.259114472677826 30.135149560469856 20.690562901209873 28-29 20.70610323577964 28.35557223066198 30.348742634501562 20.589581899056817 30-31 21.254829600877354 28.06304797332291 30.05986593574626 20.622256490053477 32-33 21.07018896980509 28.2158016054104 29.80463093773989 20.909378487044613 34-35 21.213603692147913 28.436597371953948 29.712970273503668 20.63682866239447 36-37 21.089240963739904 28.393940438305286 29.808211311938486 20.708607286016317 38-39 20.921436668070083 28.415454869320207 30.059396962140106 20.603711500469604 40-41 21.25165273099952 28.28130744721949 29.819814427748938 20.647225394032056 42-43 21.228720705913197 28.449340343826446 29.467207359670528 20.85473159058983 44-45 21.3672075319838 28.586835716338392 29.272934906737724 20.77302184494008 46-47 21.197373937657968 28.57900238445549 29.16410350226293 21.059520175623618 48-49 21.162875797751447 28.688860220314936 29.275670453302677 20.87259352863094 50-51 21.321069432470676 28.71397731769147 29.046202370430024 20.918750879407824 52-53 21.308410149561382 28.868349865911107 28.838632938428958 20.984607046098546 54-55 21.40078335103157 28.781609100115208 28.838887661223865 20.97871988762936 56-57 21.236283376394077 29.07332124644072 28.760982950951693 20.92941242621351 58-59 21.167297331258897 28.977002009231324 28.99329867592822 20.86240198358156 60-61 21.301205006567816 28.914950009456618 28.939252227487415 20.844592756488147 62-63 21.240202988660066 28.90378402802481 28.94933133802607 20.90668164528905 64-65 21.250193386179696 28.82173201974988 28.857986259668984 21.070088334401436 66-67 21.115364722880678 29.058686713633175 28.725742406983407 21.10020615650274 68-69 21.2233291889026 29.03072666088623 28.815411477706366 20.930532672504803 70-71 21.182934052455202 28.834347880385184 28.923426268848836 21.059291798310774 72-73 21.172812972133762 28.81323434572544 28.89163883255564 21.122313849585165 74-75 21.22972614493138 28.990110213504988 28.626470929581906 21.153692711981726 76-77 21.07632126387756 28.940107403057908 28.719460559279504 21.26411077378503 78-79 20.995926253290598 29.000433002401337 28.840343903929956 21.16329684037811 80-81 21.018168644951913 29.09939866278524 28.6537208744292 21.22871181783364 82-83 21.141025342009584 28.998421793247143 28.726757731010338 21.13379513373294 84-85 21.133615512878816 28.98753512668929 28.652650971886988 21.22619838854491 86-87 21.012188817178107 29.05167711231152 28.68527403783237 21.250860032678002 88-89 21.052540209555566 29.05061037890287 28.660213514722216 21.236635896819354 90-91 21.040110222847815 28.9828615080598 28.813365634549694 21.163662634542693 92-93 20.98939361543595 28.887515307014986 28.90674545324962 21.21634562429944 94-95 20.925861533781724 29.05926589616044 28.771349522497225 21.243523047560615 96-97 20.979594903194634 28.96606663359296 28.796354465533614 21.257983997678796 98-99 20.854088377822773 29.00247544952531 28.70718320787688 21.43625296477504 100-101 20.735406789343326 29.014759351857517 28.80370041294253 21.44613344585663 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2794.0 1 1570.5 2 336.5 3 287.5 4 236.0 5 229.0 6 316.0 7 564.5 8 833.5 9 997.5 10 990.0 11 951.5 12 1011.0 13 1134.0 14 1248.0 15 1374.0 16 1550.0 17 1702.5 18 1891.0 19 2119.5 20 2462.5 21 2947.5 22 3672.5 23 4576.5 24 5829.5 25 7686.0 26 10466.0 27 14208.0 28 18228.5 29 23592.0 30 29816.0 31 36085.0 32 43112.0 33 50088.5 34 56801.5 35 62846.5 36 68460.0 37 73265.0 38 75600.0 39 77097.0 40 78730.0 41 79071.0 42 79082.0 43 80064.0 44 80638.0 45 80331.0 46 79399.5 47 76796.0 48 73972.5 49 70808.0 50 65976.5 51 60579.5 52 55401.0 53 49912.5 54 43453.5 55 37290.5 56 31765.5 57 26408.0 58 22004.0 59 18324.0 60 14557.0 61 10971.5 62 7840.5 63 5393.0 64 3787.0 65 2784.0 66 2041.5 67 1587.5 68 1336.5 69 1000.0 70 718.5 71 552.5 72 433.5 73 337.5 74 254.5 75 188.5 76 135.5 77 108.0 78 71.5 79 54.5 80 52.0 81 39.0 82 27.5 83 20.5 84 19.0 85 18.5 86 12.0 87 9.5 88 9.5 89 7.0 90 5.0 91 5.0 92 3.5 93 2.0 94 2.0 95 4.5 96 3.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14368663890243283 2 0.14829330708050076 3 0.14120214370527262 4 0.145446489667088 5 0.13411098033004448 6 0.11454558065240775 7 0.12148146307781335 8 0.10957659025808729 9 0.10802378075986216 10-11 0.09479901986664473 12-13 0.11397621716972518 14-15 0.1331275343145019 16-17 0.1438678000105591 18-19 0.14068454053919757 20-21 0.1320923279823518 22-23 0.12484588365730116 24-25 0.10921426804183477 26-27 0.11356213463686515 28-29 0.11866052582270435 30-31 0.10799790060155841 32-33 0.10716973553583833 34-35 0.12285311146791222 36-37 0.12611401141418502 38-39 0.11229400687998128 40-41 0.11498554334357153 42-43 0.10936954899165728 44-45 0.12683865584669007 46-47 0.1242765201746186 48-49 0.13732011995970977 50-51 0.12507880508203492 52-53 0.10898134661710097 54-55 0.10044089437686272 56-57 0.1213003019696871 58-59 0.11074119738175615 60-61 0.10957659025808729 62-63 0.10975775136621356 64-65 0.13211820814065556 66-67 0.123189553525861 68-69 0.128624386769649 70-71 0.11495966318526778 72-73 0.11622779094215163 74-75 0.09886220472033383 76-77 0.12567404872302124 78-79 0.12585520983114748 80-81 0.12676101537177883 82-83 0.13338633589753943 84-85 0.12212846703540717 86-87 0.13196292719083305 88-89 0.11796176154850303 90-91 0.12611401141418502 92-93 0.14076218101410884 94-95 0.11671951394992292 96-97 0.10090673722633027 98-99 0.10504756255493063 100-101 0.1501049181617634 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1931982.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.37393463162237 #Duplication Level Percentage of deduplicated Percentage of total 1 61.56985180270874 32.86225245396453 2 20.278470991595096 21.646835702692943 3 8.647804840733214 13.847021108289661 4 4.09110658686178 8.734338221526414 5 2.1456196507304126 5.7260081491204735 6 1.148765825895653 3.6788491259037763 7 0.6803854139737187 2.542039262681981 8 0.4165493060072284 1.7786300343741976 9 0.25598722291812287 1.229674077230619 >10 0.7401894338771784 5.949561014546887 >50 0.018157774186560116 0.660581538325571 >100 0.0064296997294101136 0.5764025229701651 >500 2.9205033551285174E-4 0.10630931102461885 >1k 3.8940044735046356E-4 0.6614974773481954 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4668 0.24161715792383157 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4580 0.2370622500623712 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2053 0.106263929995207 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.1760316607504624E-5 0.0 22-23 0.0 0.0 0.0 1.2940079151876156E-4 0.0 24-25 0.0 0.0 0.0 3.6232221625253237E-4 0.0 26-27 0.0 0.0 0.0 5.434833243787985E-4 0.0 28-29 0.0 0.0 0.0 0.0012940079151876156 0.0 30-31 0.0 0.0 0.0 0.0026915364635902404 0.0 32-33 0.0 0.0 0.0 0.004684308652979169 0.0 34-35 0.0 0.0 0.0 0.006780601475583106 0.0 36-37 0.0 0.0 0.0 0.008773373664972033 0.0 38-39 0.0 0.0 0.0 0.010429703796412181 0.0 40-41 0.0 0.0 0.0 0.011749591869903549 0.0 42-43 0.0 0.0 0.0 0.014803450549746324 0.0 44-45 0.0 0.0 0.0 0.019125436986472958 0.0 46-47 0.0 0.0 0.0 0.02248985756596076 0.0 48-49 0.0 0.0 0.0 0.029995103474048927 0.0 50-51 0.0 0.0 0.0 0.03625810178355699 0.0 52-53 0.0 0.0 0.0 0.04619608257219788 0.0 54-55 0.0 0.0 0.0 0.06552856082510086 0.0 56-57 0.0 0.0 0.0 0.0851974811359526 0.0 58-59 0.0 0.0 0.0 0.10352063321500926 0.0 60-61 0.0 0.0 0.0 0.12795150265375144 0.0 62-63 0.0 0.0 0.0 0.16250151398926077 0.0 64-65 0.0 0.0 0.0 0.20183935461096428 0.0 66-67 0.0 0.0 0.0 0.2645210980226524 0.0 68-69 0.0 0.0 0.0 0.3377101857056639 0.0 70-71 0.0 0.0 0.0 0.41227092177877434 0.0 72-73 0.0 0.0 0.0 0.49573443230837555 0.0 74-75 0.0 0.0 0.0 0.5930179473721805 0.0 76-77 0.0 0.0 0.0 0.7017404924062439 0.0 78-79 0.0 0.0 0.0 0.8140344992862252 0.0 80-81 0.0 0.0 0.0 0.9306763727612369 0.0 82-83 0.0 0.0 0.0 1.0583949539902546 0.0 84-85 0.0 0.0 0.0 1.2063000586961992 0.0 86-87 0.0 0.0 0.0 1.3788430741073157 0.0 88-89 0.0 0.0 0.0 1.5701750844469566 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3690 0.0 56.391193 1 GTATCAA 6155 0.0 36.04561 1 GTGGTAT 1700 0.0 34.37318 1 TGGTATC 1725 0.0 32.222576 2 CGGTCCG 30 0.0039514895 31.667662 44-45 ATCAACG 6990 0.0 31.5342 3 TCAACGC 7030 0.0 31.353962 4 CAACGCA 7200 0.0 30.547682 5 TATCAAC 7265 0.0 30.472902 2 AACGCAG 7495 0.0 29.53012 6 ACGCAGA 8255 0.0 26.640194 7 CGCAGAG 8330 0.0 26.34468 8 GCAGAGT 9205 0.0 22.911587 9 GAGTACT 6170 0.0 22.905365 12-13 CGGAGAT 275 5.2750693E-11 22.458162 1 CAGAGTA 9025 0.0 22.027864 10-11 GTACTTT 6655 0.0 21.093588 14-15 TACATGG 4775 0.0 19.003057 2 AGAGTAC 8335 0.0 18.608084 10-11 GTACATG 5115 0.0 18.482937 1 >>END_MODULE