FastQCFastQC Report
Fri 10 Feb 2017
SRR2031703_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031703_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931982
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58700.3038330584860522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54990.2846299810246679No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26040.13478386444594204No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT21650.11206108545524752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38750.056.642521
GTATCAA63600.034.435382
TGGTATC17950.032.5538022
GTGGTAT20200.030.3396551
ATCAACG72700.029.9282194
TCAACGC72850.029.8665964
CAACGCA74150.029.33695
TATCAAC76950.028.5846843
AACGCAG77150.028.3192546
CGCAGAG85150.025.379718
ACGCAGA85800.025.353517
CGGAGAT1652.4274832E-623.0344751
GCAGAGT95200.022.4509899
GAGTACT70000.021.95036712-13
CAGAGTA93850.021.71115510-11
GCGGTAT1100.002875042821.5948221
GTACTTT74000.020.5715714-15
AGAGTAC87550.018.87917310-11
AGTACTT75300.017.22000112-13
TACATGG39850.016.8093852
Produced by FastQC (version 0.11.5)