##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031703_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1931982 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.7329271183686 34.0 31.0 34.0 31.0 34.0 2 32.897023885315704 34.0 31.0 34.0 31.0 34.0 3 32.949271266502485 34.0 31.0 34.0 31.0 34.0 4 36.35524192254379 37.0 37.0 37.0 35.0 37.0 5 36.324613272794466 37.0 37.0 37.0 35.0 37.0 6 36.3533971848599 37.0 37.0 37.0 35.0 37.0 7 36.30360634829931 37.0 37.0 37.0 35.0 37.0 8 36.29518805040627 37.0 37.0 37.0 35.0 37.0 9 38.143975979072266 39.0 39.0 39.0 37.0 39.0 10-11 38.10744277120594 39.0 39.0 39.0 36.0 39.0 12-13 38.06047649512263 39.0 39.0 39.0 35.0 39.0 14-15 39.53786655362214 41.0 39.0 41.0 37.0 41.0 16-17 39.51380861726455 41.0 39.0 41.0 37.0 41.0 18-19 39.539595348196826 41.0 39.5 41.0 37.0 41.0 20-21 39.544525518353694 41.0 39.5 41.0 37.0 41.0 22-23 39.457731749053565 41.0 39.0 41.0 36.0 41.0 24-25 39.39782617022311 41.0 39.0 41.0 36.0 41.0 26-27 39.27090521547302 41.0 39.0 41.0 36.0 41.0 28-29 39.16677096370463 41.0 39.0 41.0 36.0 41.0 30-31 38.99896608767577 40.0 39.0 41.0 35.5 41.0 32-33 38.78090712025268 40.0 38.0 41.0 35.0 41.0 34-35 38.63543837364944 40.0 38.0 41.0 35.0 41.0 36-37 38.51971938661954 40.0 38.0 41.0 35.0 41.0 38-39 38.58557119062186 40.0 38.0 41.0 35.0 41.0 40-41 38.50714137088234 40.0 38.0 41.0 35.0 41.0 42-43 38.406318485368914 40.0 38.0 41.0 35.0 41.0 44-45 38.22091587809824 40.0 38.0 41.0 34.0 41.0 46-47 38.0022595966215 40.0 38.0 41.0 33.5 41.0 48-49 37.75614705520031 40.0 38.0 41.0 33.0 41.0 50-51 37.62605034622476 40.0 38.0 41.0 33.0 41.0 52-53 37.70207900487685 40.0 38.0 41.0 33.0 41.0 54-55 37.65916685559182 40.0 38.0 41.0 33.0 41.0 56-57 37.515826752009076 40.0 38.0 41.0 33.0 41.0 58-59 37.375397131029175 40.0 37.5 41.0 33.0 41.0 60-61 37.166506468486766 40.0 37.0 41.0 32.5 41.0 62-63 36.95064576170999 40.0 37.0 41.0 32.0 41.0 64-65 36.68017455649173 39.0 36.0 41.0 31.5 41.0 66-67 36.376397399147606 39.0 36.0 41.0 31.0 41.0 68-69 36.022213975078444 39.0 35.0 40.5 31.0 41.0 70-71 35.68062901207154 38.0 35.0 40.0 31.0 41.0 72-73 35.28945404253248 37.0 35.0 39.5 30.0 41.0 74-75 34.88283043009717 37.0 35.0 39.0 30.0 41.0 76-77 33.90808221815731 36.0 34.0 39.0 28.5 39.0 78-79 34.072446844742856 36.0 34.0 37.5 29.0 39.0 80-81 33.79965522453108 35.5 34.0 37.0 29.0 39.0 82-83 33.47558491745782 35.0 34.0 37.0 29.0 39.0 84-85 33.13850750162268 35.0 34.0 36.0 29.0 37.5 86-87 32.85046884494783 35.0 34.0 36.0 29.0 37.0 88-89 32.58802980566071 35.0 34.0 36.0 29.0 37.0 90-91 32.358960901292036 35.0 34.0 35.0 28.5 36.0 92-93 32.174149138035446 35.0 34.0 35.0 27.5 36.0 94-95 31.986118659490614 35.0 34.0 35.0 27.0 36.0 96-97 31.814926329541372 35.0 34.0 35.0 26.5 36.0 98-99 31.611415893108735 35.0 34.0 35.0 25.5 35.5 100-101 30.92877392232433 34.5 32.5 35.0 22.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20398518696722334 1101 2 0.19005207743003183 1101 3 0.22483850408960393 1101 4 0.15474798076839136 1101 5 0.15096029900013264 1101 6 0.12379860611510196 1101 7 0.15606587999848642 1101 8 0.15422101250472764 1101 9 0.132012886038126 1101 10-11 0.20468924453206228 1101 12-13 0.19147793372038535 1101 14-15 0.15888219841956186 1101 16-17 0.13030336841828216 1101 18-19 0.19312130276164652 1101 20-21 0.1365765831101342 1101 22-23 -0.044759602450120894 1101 24-25 0.026794441783962952 1101 26-27 0.16878574480136166 1101 28-29 0.07804158545151552 1101 30-31 0.236763532773395 1101 32-33 0.16855484195080805 1101 34-35 -0.025160296939155558 1101 36-37 0.2703543888546278 1101 38-39 0.23511492623716634 1101 40-41 -0.1502706006022123 1101 42-43 0.07471149300821622 1101 44-45 0.08654066193168575 1101 46-47 0.11489667757208366 1101 48-49 0.08911003720722022 1101 50-51 0.3109960090281021 1101 52-53 0.24072324439082848 1101 54-55 0.3014517670635186 1101 56-57 0.04606091573541704 1101 58-59 0.039907157206791055 1101 60-61 0.07965476351176193 1101 62-63 0.05952999478137144 1101 64-65 0.1545436327022145 1101 66-67 0.1156346589190207 1101 68-69 -0.0026985278588895767 1101 70-71 -0.08260602447246157 1101 72-73 -0.15507216728200746 1101 74-75 -0.026064065974757966 1101 76-77 -0.12675424524405088 1101 78-79 -0.22559113200880887 1101 80-81 -0.13030155318939052 1101 82-83 -0.05577029656558352 1101 84-85 -0.1577634108638506 1101 86-87 -0.04168694742102019 1101 88-89 -0.05136329866223832 1101 90-91 -0.20593607802690883 1101 92-93 -0.14758576482054053 1101 94-95 -0.20028202041881826 1101 96-97 -0.06758271489939105 1101 98-99 -0.26979031216077587 1101 100-101 -0.4210200978831118 1102 1 0.11118874867882056 1102 2 0.1179056515086856 1102 3 0.17493694584077701 1102 4 0.07222905917551259 1102 5 0.06076193145128883 1102 6 0.10504025835354014 1102 7 0.06382150700813582 1102 8 0.04594300112703564 1102 9 0.14548209207325158 1102 10-11 0.15073327124490277 1102 12-13 0.11908119862268762 1102 14-15 0.1625062399728705 1102 16-17 0.20807136248209446 1102 18-19 0.10134489910099376 1102 20-21 0.1257301819228971 1102 22-23 0.11919191869754542 1102 24-25 0.08648576011194109 1102 26-27 0.21923837898389564 1102 28-29 0.20328026959695222 1102 30-31 0.10464085159605219 1102 32-33 0.27215810685310515 1102 34-35 0.09381274288276131 1102 36-37 0.24549858870621932 1102 38-39 0.11159575730320626 1102 40-41 0.22647735883385423 1102 42-43 0.4238314534336425 1102 44-45 0.3202908474374908 1102 46-47 0.4489189382695926 1102 48-49 0.33125794717506807 1102 50-51 0.42903014209760215 1102 52-53 0.587694305484078 1102 54-55 0.5950871863066496 1102 56-57 0.6791531739595058 1102 58-59 0.6718339442461172 1102 60-61 0.7516517954187663 1102 62-63 0.6840108258474089 1102 64-65 0.7213118741071085 1102 66-67 0.619349721991 1102 68-69 0.67486367842357 1102 70-71 0.6422487863116046 1102 72-73 0.590282272490434 1102 74-75 0.6523806532931076 1102 76-77 0.6132685101356188 1102 78-79 0.605352226883106 1102 80-81 0.4635554836644289 1102 82-83 0.5525967576021102 1102 84-85 0.6568811599993722 1102 86-87 0.6004510689034888 1102 88-89 0.49462037682626203 1102 90-91 0.3921504913522611 1102 92-93 0.4537627387659029 1102 94-95 0.48683645596010905 1102 96-97 0.3969317545539859 1102 98-99 0.3179475062908672 1102 100-101 0.5211367163637348 1103 1 0.14978215527222716 1103 2 0.08010528520831883 1103 3 0.09200471140854205 1103 4 0.05520158664803887 1103 5 0.028498195187552255 1103 6 0.07881315212643614 1103 7 0.06408341177003507 1103 8 0.13229098647502013 1103 9 0.1256432642344265 1103 10-11 0.11374700750863553 1103 12-13 0.04893467847616506 1103 14-15 0.11890459162121658 1103 16-17 0.14485799251873033 1103 18-19 0.13789526540136166 1103 20-21 0.16276131745403433 1103 22-23 0.09518642419204326 1103 24-25 0.05296195058812714 1103 26-27 0.052209990705513576 1103 28-29 0.043106276922955544 1103 30-31 0.06367748163268061 1103 32-33 0.0846425390875396 1103 34-35 0.019451937021955246 1103 36-37 -0.012607638400005783 1103 38-39 -0.19300863830118686 1103 40-41 -0.02368106607457321 1103 42-43 0.02243676478895651 1103 44-45 0.08431282545947738 1103 46-47 -0.003634175283529828 1103 48-49 0.21159128050840081 1103 50-51 0.041847907665371054 1103 52-53 -0.04744855165878192 1103 54-55 0.12096447568394098 1103 56-57 0.10772643403277016 1103 58-59 0.19267185633403017 1103 60-61 0.08672871849568509 1103 62-63 -0.04508166499508093 1103 64-65 0.0872587605539934 1103 66-67 0.10693671832799367 1103 68-69 0.0811860227459178 1103 70-71 0.12801160682442259 1103 72-73 0.22517970838787704 1103 74-75 0.33604548845794113 1103 76-77 0.4237740046411176 1103 78-79 0.3028558898867644 1103 80-81 0.2669748975838431 1103 82-83 0.3771553656607196 1103 84-85 0.24648555560376906 1103 86-87 0.281755098207519 1103 88-89 0.2720672632731507 1103 90-91 0.23714499684677293 1103 92-93 0.25961163986480784 1103 94-95 0.38550403837768954 1103 96-97 0.2571579450301691 1103 98-99 0.2765042828476467 1103 100-101 0.297501185228203 1104 1 0.17129748205071849 1104 2 0.10533422293725891 1104 3 0.1345991783451126 1104 4 0.09563680932010499 1104 5 0.12844035828760525 1104 6 0.1320616580065206 1104 7 0.15591635952403493 1104 8 0.18203554016694312 1104 9 0.10975739571697574 1104 10-11 0.11136485019227393 1104 12-13 0.20242417143933977 1104 14-15 0.283063787688306 1104 16-17 0.2457532595324139 1104 18-19 0.15736340690897777 1104 20-21 0.22529987538732854 1104 22-23 0.14986398347486585 1104 24-25 0.2059212719685064 1104 26-27 0.271557638671581 1104 28-29 0.3755069902447232 1104 30-31 0.3060381910986578 1104 32-33 0.3655623867837008 1104 34-35 0.36204640395852294 1104 36-37 0.3346113706611078 1104 38-39 0.40503936632575943 1104 40-41 0.3277058145886187 1104 42-43 0.392555571420921 1104 44-45 0.45247361204131664 1104 46-47 0.5124493026420609 1104 48-49 0.4707536094075735 1104 50-51 0.434908684607727 1104 52-53 0.42279436332097475 1104 54-55 0.4535001417722313 1104 56-57 0.3693926187252714 1104 58-59 0.4116025966463468 1104 60-61 0.5368733107455412 1104 62-63 0.4999576158994614 1104 64-65 0.5247635802956552 1104 66-67 0.41023462656011134 1104 68-69 0.5329596876774758 1104 70-71 0.494380654443475 1104 72-73 0.41196879649275076 1104 74-75 0.45055532073093474 1104 76-77 0.43782837132706476 1104 78-79 0.6058658185545838 1104 80-81 0.6227272592572604 1104 82-83 0.5741791751845255 1104 84-85 0.6612050466390542 1104 86-87 0.6342902823216505 1104 88-89 0.572043164564839 1104 90-91 0.5464639673499505 1104 92-93 0.6252745953303958 1104 94-95 0.6470099666599367 1104 96-97 0.6260838656008296 1104 98-99 0.6932095074476052 1104 100-101 0.8081696833967023 1105 1 0.08653570195649252 1105 2 0.06428404909262753 1105 3 0.0726285429272906 1105 4 0.057292480742397345 1105 5 0.047947221911400106 1105 6 0.06537424307551731 1105 7 0.09156746543769145 1105 8 0.12574474211122322 1105 9 0.04691787107485368 1105 10-11 0.13432396230903265 1105 12-13 0.20710304975077065 1105 14-15 0.12423905219528564 1105 16-17 0.1404486941573495 1105 18-19 0.08594802576647709 1105 20-21 0.08764721869675185 1105 22-23 0.13719813649822044 1105 24-25 0.20884834671701924 1105 26-27 0.18019236725890408 1105 28-29 0.27980896030577895 1105 30-31 0.1478218784214178 1105 32-33 -0.06258678566776865 1105 34-35 -0.01446563693603764 1105 36-37 0.17284820675561008 1105 38-39 0.12816424193635356 1105 40-41 0.0759264616940456 1105 42-43 0.084841224263279 1105 44-45 0.15881793928329557 1105 46-47 0.2825694978716484 1105 48-49 0.17987457535058837 1105 50-51 0.21243998398781372 1105 52-53 0.1849252523662912 1105 54-55 0.19602358708831247 1105 56-57 0.10999328765643668 1105 58-59 0.07557972502423382 1105 60-61 0.08551624736404762 1105 62-63 0.05165552295169107 1105 64-65 0.043410470660674605 1105 66-67 0.10339484545875166 1105 68-69 0.10381606783554531 1105 70-71 0.02749678773282227 1105 72-73 -0.005286034491270186 1105 74-75 0.1765313017208925 1105 76-77 0.05638700365134497 1105 78-79 0.16381074527002681 1105 80-81 0.06797154335531985 1105 82-83 0.19328178158505693 1105 84-85 0.22906556220225127 1105 86-87 0.03872749455636182 1105 88-89 0.031885255135016166 1105 90-91 0.14272483848319695 1105 92-93 0.1677339592313558 1105 94-95 0.20160639733512653 1105 96-97 0.23442136194428187 1105 98-99 0.27351297084940285 1105 100-101 0.3268135683800786 1106 1 0.07235612918250922 1106 2 0.09296852627311836 1106 3 0.016314023106495767 1106 4 0.06415402062831532 1106 5 0.026223298591943944 1106 6 -0.07537605139462045 1106 7 0.04542952482550788 1106 8 -0.04146285804719696 1106 9 0.054235903962918997 1106 10-11 0.03179306223341882 1106 12-13 0.0668368950420799 1106 14-15 0.11172792919678898 1106 16-17 0.09941640274250574 1106 18-19 0.12454483057264554 1106 20-21 0.15852836592153352 1106 22-23 0.18618626510836833 1106 24-25 0.17634990265091233 1106 26-27 0.06918355630102013 1106 28-29 0.12575157334887166 1106 30-31 0.19244246201235882 1106 32-33 0.2626220438070206 1106 34-35 0.25477779342242 1106 36-37 0.22480353987976542 1106 38-39 0.022207715500911718 1106 40-41 0.16280543832673544 1106 42-43 0.14702778716672782 1106 44-45 0.11580343952246608 1106 46-47 0.19458143612808243 1106 48-49 0.12085260196348457 1106 50-51 0.04011323272634826 1106 52-53 0.13747826984933198 1106 54-55 0.06567361769263158 1106 56-57 0.07369180681885013 1106 58-59 0.12449455757488437 1106 60-61 0.12358840669167392 1106 62-63 0.10848247224110708 1106 64-65 0.10510132074065126 1106 66-67 0.13036908125089042 1106 68-69 0.16314858505369045 1106 70-71 0.11110830318671105 1106 72-73 0.11224355397065011 1106 74-75 0.23686874649166612 1106 76-77 0.10245679178715505 1106 78-79 0.05476356832562601 1106 80-81 0.0676841456483217 1106 82-83 0.13109136099426166 1106 84-85 0.012226885543313415 1106 86-87 0.014118021104962963 1106 88-89 0.0938352132148168 1106 90-91 0.05899116293013407 1106 92-93 0.26310949223515223 1106 94-95 0.23898995947218538 1106 96-97 0.33412329130230844 1106 98-99 0.37894356591053935 1106 100-101 0.324689911498222 1107 1 0.17691903512877616 1107 2 0.12616276677835003 1107 3 0.16461628741969747 1107 4 0.1048205346456541 1107 5 0.09520471042962697 1107 6 0.14763164186542355 1107 7 0.15538396965009582 1107 8 0.1258006497545594 1107 9 0.1687195742563432 1107 10-11 0.11119545341419723 1107 12-13 0.1424891714030352 1107 14-15 0.2404713877957505 1107 16-17 0.1818362252344059 1107 18-19 0.19944761646731024 1107 20-21 0.2181682710304429 1107 22-23 0.23424051863317175 1107 24-25 0.2902203685996909 1107 26-27 0.35677389785832503 1107 28-29 0.3176156413381861 1107 30-31 0.26732860735493347 1107 32-33 0.3744375679777576 1107 34-35 0.3920635130331078 1107 36-37 0.33306381009571595 1107 38-39 0.32457952667191137 1107 40-41 0.3923766645490545 1107 42-43 0.38265916965048774 1107 44-45 0.4159625564815883 1107 46-47 0.48425683886272 1107 48-49 0.3523912406598271 1107 50-51 0.4258798363364136 1107 52-53 0.41749466412125 1107 54-55 0.2914491339824039 1107 56-57 0.41475223598534683 1107 58-59 0.3706642352939795 1107 60-61 0.4313790472464305 1107 62-63 0.32705795445102126 1107 64-65 0.3562839647779441 1107 66-67 0.36430995004745625 1107 68-69 0.34507325127749766 1107 70-71 0.4495510071033664 1107 72-73 0.32397927005354177 1107 74-75 0.2540296486492153 1107 76-77 0.34373814092324295 1107 78-79 0.402599364682537 1107 80-81 0.4959821699488529 1107 82-83 0.37902077709726 1107 84-85 0.4161508275702914 1107 86-87 0.46171126477456426 1107 88-89 0.44851177413188026 1107 90-91 0.4558016017481421 1107 92-93 0.5026966667625956 1107 94-95 0.38293006945957586 1107 96-97 0.5034756876083755 1107 98-99 0.6158447889245551 1107 100-101 0.710280125256098 1108 1 0.06505982867720661 1108 2 0.02186745298968873 1108 3 -0.007483966229791861 1108 4 0.019899677878221667 1108 5 0.020562522233468883 1108 6 0.027827455375351917 1108 7 -0.015185331497917787 1108 8 0.02961507655292195 1108 9 0.04765924579800895 1108 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9 563.0 10 1070.0 11 1881.0 12 2779.0 13 4201.0 14 5635.0 15 6895.0 16 8442.0 17 11859.0 18 11787.0 19 7161.0 20 4924.0 21 4650.0 22 4932.0 23 5335.0 24 6107.0 25 7226.0 26 7618.0 27 9218.0 28 12138.0 29 15640.0 30 20509.0 31 26718.0 32 34418.0 33 46054.0 34 64241.0 35 96503.0 36 161007.0 37 360234.0 38 716186.0 39 265353.0 40 295.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.77603205968792 20.74012487788918 14.99942794930958 23.484415113113315 2 15.598540773154202 22.10087878665536 40.0240271389692 22.276553301221234 3 17.692386528802075 26.24713759819125 32.27210519559573 23.78837067741094 4 12.82273891856157 19.877347174486225 38.59060037864375 28.70931352830846 5 13.037957910580946 37.2548502004677 36.00794417339292 13.699247715558426 6 28.291464413229523 38.023128579872896 19.477976502886673 14.207430504010906 7 24.147067622783236 33.69135944330745 24.28387013957687 17.877702794332453 8 23.146488942443565 35.160783071477894 22.652592001374757 19.04013598470379 9 24.509803921568626 16.933335817828528 22.776661480282943 35.7801987803199 10-11 22.58015928206352 27.170058305423733 30.435591645158095 19.814190767354656 12-13 23.7411952443644 25.778818275433796 29.511100390971652 20.96888608923015 14-15 21.259370563168066 27.6390399284465 27.968623915038503 23.13296559334693 16-17 20.21358614733583 29.794190926781372 29.08605015475213 20.906172771130674 18-19 19.883037152021682 28.426702954118078 31.37872473901141 20.31153515484883 20-21 20.896364458882122 27.72882977170595 31.14423426305214 20.23057150635979 22-23 20.577520908579892 27.93100556837486 30.668375792321044 20.8230977307242 24-25 20.3761732769767 28.042083207814567 31.193303043196053 20.38844047201268 26-27 20.22032410407604 28.205740041781148 31.203583695829963 20.370352158312855 28-29 19.922593014943576 28.19805918316323 31.35021717333543 20.529130628557763 30-31 20.53184774284847 27.941390743130825 31.119164443344825 20.407597070675884 32-33 20.384838352363897 28.04278512199919 30.94133997246348 20.63103655317343 34-35 20.36169136136394 28.234289241791476 30.960097217171413 20.443922179673173 36-37 20.366172818289954 28.262510210145237 30.950586520434264 20.420730451130545 38-39 20.1582105837425 28.26972508025437 31.120709199154028 20.4513551368491 40-41 20.57832319348731 28.066617597886523 30.93279336971048 20.422265838915685 42-43 20.54550715275815 28.261262268489045 30.546609647501892 20.64662093125091 44-45 20.569575699980643 28.42366026184509 30.420081553554844 20.586682484619423 46-47 20.564347908003285 28.43944715841038 30.16298806096537 20.833216872620966 48-49 20.531661268065644 28.506399127942185 30.262833711701248 20.699105892290923 50-51 20.6127438040313 28.586627618683817 30.04676027002322 20.753868307261662 52-53 20.735622795657516 28.673999033117287 29.832420798951542 20.757957372273655 54-55 20.693826339996956 28.672316822827543 29.83583697984764 20.798019857327862 56-57 20.733293581410177 28.922319152041787 29.622791516691148 20.721595749856885 58-59 20.534100008333425 28.93289730374916 29.899238035196035 20.633764652721382 60-61 20.695963775535027 28.965001237089496 29.71654604612739 20.622488941248083 62-63 20.622805970890962 29.03405648239432 29.668048879662056 20.67508866705266 64-65 20.634145445106043 28.886452648466808 29.649882747302776 20.82951915912437 66-67 20.4833627303938 29.07904595646992 29.47024469655893 20.967346616577352 68-69 20.587982572696152 29.01396596427061 29.60144126722194 20.796610195811297 70-71 20.665317962906936 28.74835304844231 29.6474831371053 20.938845851545448 72-73 20.691540107245295 28.711358590519986 29.638069129163856 20.95903217307086 74-75 20.728619154942994 28.820102637580842 29.366108892737824 21.085169314738337 76-77 20.581496433778057 28.92528338647059 29.384949324424873 21.10827085532648 78-79 20.595002985289394 28.93929445719623 29.404518229675507 21.061184327838866 80-81 20.58577267241408 28.927653624850496 29.291894786508816 21.194678916226604 82-83 20.734971722897335 28.875257211631787 29.25376920189981 21.136001863571067 84-85 20.71317006890751 28.845990100611434 29.232855617741254 21.207984212739802 86-87 20.610435526619884 28.88780420389053 29.22409681210446 21.27766345738512 88-89 20.694916004326146 28.824888734297478 29.21653469028706 21.263660571089318 90-91 20.70798278658911 28.80534083650883 29.258295367141102 21.22838100976096 92-93 20.740850587634874 28.71375095627185 29.248279745877547 21.297118710215727 94-95 20.726797661675935 28.780495884537228 29.11010558069382 21.382600873093022 96-97 20.743340451241384 28.677681437425772 29.126107056408152 21.452871054924696 98-99 20.68472723075434 28.65944333821088 29.011465433666274 21.644363997368504 100-101 20.637289307668258 28.821008051909867 28.881309382576486 21.660393257845385 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1086.0 1 634.0 2 191.0 3 209.5 4 171.0 5 154.0 6 264.0 7 525.5 8 853.5 9 1050.5 10 993.5 11 882.0 12 941.5 13 1052.0 14 1183.0 15 1353.0 16 1492.5 17 1677.0 18 1941.0 19 2194.5 20 2537.5 21 2957.0 22 3582.0 23 4633.0 24 5969.5 25 7794.0 26 10725.5 27 14523.0 28 18813.5 29 24648.0 30 31414.0 31 38261.5 32 45515.5 33 52659.0 34 59830.0 35 66557.5 36 72443.0 37 77224.5 38 79843.5 39 81934.0 40 84029.5 41 84415.5 42 84725.0 43 85811.5 44 85949.0 45 84873.0 46 82924.5 47 79738.0 48 75856.5 49 70812.0 50 64344.5 51 57513.5 52 50493.5 53 43616.5 54 36585.0 55 29775.5 56 24125.0 57 19471.5 58 16021.0 59 13191.5 60 10285.5 61 7692.5 62 5494.5 63 3846.0 64 2689.5 65 1894.0 66 1404.0 67 1071.0 68 872.0 69 672.0 70 457.0 71 333.5 72 239.0 73 162.0 74 130.0 75 93.5 76 66.0 77 54.5 78 33.0 79 19.5 80 11.5 81 5.5 82 5.0 83 4.0 84 2.0 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017339706063514048 2 0.0 3 0.0019668920310851757 4 0.018167871129234122 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.5880158303752312E-5 12-13 2.5880158303752312E-5 14-15 5.1760316607504624E-5 16-17 0.0011128468070613494 18-19 5.1760316607504624E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.176031660750462E-4 28-29 0.005952436409863032 30-31 2.5880158303752312E-5 32-33 1.0352063321500925E-4 34-35 0.011620191078384788 36-37 0.00414082532860037 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.5528094982251387E-4 60-61 0.0014492888650101295 62-63 0.009135695881224567 64-65 0.0022515737724264516 66-67 0.0051501515024467105 68-69 0.0036749824791328283 70-71 1.8116110812626618E-4 72-73 0.007712287174518188 74-75 0.007039403058620629 76-77 0.0018374912395664141 78-79 4.399626911637893E-4 80-81 0.008152249865681978 82-83 0.010714385537753458 84-85 0.0023033340890339558 86-87 0.001733970606351405 88-89 3.882023745562847E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 2.5880158303752312E-5 98-99 5.1760316607504624E-5 100-101 9.316856989350832E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1931982.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.65858084624585 #Duplication Level Percentage of deduplicated Percentage of total 1 58.998444934418714 29.887774925130344 2 21.060702336334092 21.33810583967799 3 9.254290550378807 14.064276780630413 4 4.507039528804213 9.13280905388616 5 2.339872947313342 5.926732143570825 6 1.3178245673144842 4.005547343068185 7 0.8046900354678305 2.853511865254087 8 0.4882622284631857 1.9787737259816363 9 0.31850304668250334 1.452142110612709 >10 0.8778869425449115 6.688044237247858 >50 0.02337428759585006 0.8051899362614676 >100 0.007983404586665783 0.7140230662944892 >500 6.137400520686768E-4 0.2273284910051983 >1k 3.0687002603433836E-4 0.33661405916794346 >5k 2.0458001735622558E-4 0.5891264222106449 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5870 0.3038330584860522 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5499 0.2846299810246679 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2604 0.13478386444594204 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 2165 0.11206108545524752 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0352063321500925E-4 0.0 12-13 0.0 0.0 0.0 1.0352063321500925E-4 0.0 14-15 0.0 0.0 0.0 1.0352063321500925E-4 0.0 16-17 0.0 0.0 0.0 1.0352063321500925E-4 0.0 18-19 0.0 0.0 0.0 1.0352063321500925E-4 0.0 20-21 0.0 0.0 0.0 1.5528094982251387E-4 0.0 22-23 0.0 0.0 0.0 2.329214247337708E-4 0.0 24-25 0.0 0.0 0.0 5.434833243787985E-4 0.0 26-27 0.0 0.0 0.0 6.987642742013124E-4 0.0 28-29 0.0 0.0 0.0 0.001501049181617634 0.0 30-31 0.0 0.0 0.0 0.002898577730020259 0.0 32-33 0.0 0.0 0.0 0.0050466308692317 0.0 34-35 0.0 0.0 0.0 0.007246444325050647 0.0 36-37 0.0 0.0 0.0 0.009265096672743329 0.0 38-39 0.0 0.0 0.0 0.010843786329272218 0.0 40-41 0.0 0.0 0.0 0.01208603392785233 0.0 42-43 2.5880158303752312E-5 0.0 0.0 0.015191652924302607 0.0 44-45 5.1760316607504624E-5 0.0 0.0 0.01946187904442174 0.0 46-47 5.1760316607504624E-5 0.0 0.0 0.022878059940517045 0.0 48-49 5.1760316607504624E-5 0.0 0.0 0.030435066165212715 0.0 50-51 5.1760316607504624E-5 0.0 0.0 0.03693098589945455 0.0 52-53 5.1760316607504624E-5 0.0 0.0 0.04702424763791795 0.0 54-55 5.1760316607504624E-5 0.0 0.0 0.06656376715725096 0.0 56-57 5.1760316607504624E-5 0.0 0.0 0.08646560889283647 0.0 58-59 5.1760316607504624E-5 0.0 0.0 0.10494404192171564 0.0 60-61 5.1760316607504624E-5 0.0 0.0 0.12947843199367282 0.0 62-63 5.1760316607504624E-5 0.0 0.0 0.16418372427900468 0.0 64-65 5.1760316607504624E-5 0.0 0.0 0.20414268869999824 0.0 66-67 5.1760316607504624E-5 0.0 0.0 0.26736791543606514 0.0 68-69 5.1760316607504624E-5 0.0 0.0 0.341048726126848 0.0 70-71 5.1760316607504624E-5 0.0 0.0 0.41589414394129964 0.0 72-73 5.1760316607504624E-5 0.0 0.0 0.4992282536793821 0.0 74-75 5.1760316607504624E-5 0.0 0.0 0.5973399338089072 0.0 76-77 5.1760316607504624E-5 0.0 0.0 0.7073306065998545 0.0 78-79 5.1760316607504624E-5 0.0 0.0 0.8207892206035046 0.0 80-81 5.1760316607504624E-5 0.0 0.0 0.9379486972445913 0.0 82-83 5.1760316607504624E-5 0.0 0.0 1.066702484805759 0.0 84-85 5.1760316607504624E-5 0.0 0.0 1.215513395052335 0.0 86-87 5.1760316607504624E-5 0.0 0.0 1.3897386207531954 0.0 88-89 5.1760316607504624E-5 0.0 0.0 1.5828563620157952 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3875 0.0 56.64252 1 GTATCAA 6360 0.0 34.43538 2 TGGTATC 1795 0.0 32.553802 2 GTGGTAT 2020 0.0 30.339655 1 ATCAACG 7270 0.0 29.928219 4 TCAACGC 7285 0.0 29.866596 4 CAACGCA 7415 0.0 29.3369 5 TATCAAC 7695 0.0 28.584684 3 AACGCAG 7715 0.0 28.319254 6 CGCAGAG 8515 0.0 25.37971 8 ACGCAGA 8580 0.0 25.35351 7 CGGAGAT 165 2.4274832E-6 23.034475 1 GCAGAGT 9520 0.0 22.450989 9 GAGTACT 7000 0.0 21.950367 12-13 CAGAGTA 9385 0.0 21.711155 10-11 GCGGTAT 110 0.0028750428 21.594822 1 GTACTTT 7400 0.0 20.57157 14-15 AGAGTAC 8755 0.0 18.879173 10-11 AGTACTT 7530 0.0 17.220001 12-13 TACATGG 3985 0.0 16.809385 2 >>END_MODULE